MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita2 sp |
| MS data file | : | PRT1270_T-BRSC_2_20250714115216.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:39:15 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,778 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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21| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOA_PSEPK | 230 | DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1 |
22| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | OTCC_PSEPK | 229 | Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3 |
23| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ADHP_ECOLI | 207 | Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1 |
24| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL3_PSEE4 | 193 | 50S ribosomal protein L3 OS=Pseudomonas entomophila (strain L48) GN=rplC PE=3 SV=1 |
25| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PORF_PSEAE | 190 | Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1 |
26| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL10_PSEPG | 183 | 50S ribosomal protein L10 OS=Pseudomonas putida (strain GB-1) GN=rplJ PE=3 SV=1 |
27| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS16_PSEP1 | 180 | 30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1 |
28| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SUCC_PSEPK | 176 | Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1 |
29| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TIG_PSEPG | 172 | Trigger factor OS=Pseudomonas putida (strain GB-1) GN=tig PE=3 SV=1 |
30| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL5_PSEE4 | 166 | 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 30.1 | RL5_PSEE4 |
166 | 20385 | 17 (7) | 9 (3) | 0.54 | |
| 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1 | |||||||
| 4 samesets of RL5_PSEE4 | |||||||
| RL5_PSEP1 |
166 | 20316 | 17 (7) | 9 (3) | 0.55 | ||
| 50S ribosomal protein L5 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplE PE=3 SV=1 | |||||||
| RL5_PSEPG |
166 | 20316 | 17 (7) | 9 (3) | 0.55 | ||
| 50S ribosomal protein L5 OS=Pseudomonas putida (strain GB-1) GN=rplE PE=3 SV=1 | |||||||
| RL5_PSEPK |
166 | 20316 | 17 (7) | 9 (3) | 0.55 | ||
| 50S ribosomal protein L5 OS=Pseudomonas putida (strain KT2440) GN=rplE PE=3 SV=1 | |||||||
| RL5_PSEPW |
166 | 20399 | 17 (7) | 9 (3) | 0.54 | ||
| 50S ribosomal protein L5 OS=Pseudomonas putida (strain W619) GN=rplE PE=3 SV=1 | |||||||
17 peptide matches (10 non-duplicate, 7 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 286 |
581.2701 | 580.2628 | 580.2605 | 3.96 | 0 | 11 | 3.7 | 9Score > 40 indicates identity Score > 29 indicates homology |
K.SFDGR.G | ||
| 460 |
1 |
340.6807 | 679.3468 | 679.3442 | 3.91 | 1 | 15 | 3.3 | 7Score > 40 indicates identity Score > 33 indicates homology |
K.FPFRN.- | |
| 617 |
375.7302 | 749.4458 | 749.4436 | 3.06 | 1 | 21 | 0.46 | 1Score > 36 indicates identity Score > 30 indicates homology |
R.KSIAGFK.V | ||
| 922 |
2 |
441.7963 | 881.5780 | 881.5698 | 9.35 | 0 | 52 | 0.00023 | 1Score > 28 indicates identity |
U | R.LLAISLPR.V |
| 928 |
1 |
443.2482 | 884.4818 | 884.4756 | 7.06 | 1 | 34 | 0.21 | 1Score > 39 indicates identity |
R.EGWPIGVK.V | |
| 991 |
304.8429 | 911.5069 | 911.5018 | 5.61 | 1 | 28 | 0.079 | 1Score > 38 indicates identity Score > 30 indicates homology |
R.AFKFPFR.N | ||
| 1180 |
487.2288 | 972.4430 | 972.4375 | 5.72 | 1 | 22 | 0.83 | 1Score > 39 indicates identity Score > 34 indicates homology |
K.MYEFLDR.L | ||
| 1591 |
2 |
581.3330 | 1160.6514 | 1160.6401 | 9.77 | 0 | 52 | 0.003 | 1Score > 39 indicates identity |
U | R.GLDITLTTTAR.S |
| 1911 |
1 |
439.5962 | 1315.7668 | 1315.7612 | 4.21 | 0 | 49 | 0.00014 | 1Score > 34 indicates identity Score > 23 indicates homology |
U | K.ITGQKPVVTFAR.K |
| 1912 |
658.8914 | 1315.7682 | 1315.7612 | 5.33 | 0 | 38 | 0.0018 | 1Score > 34 indicates identity Score > 23 indicates homology |
U | K.ITGQKPVVTFAR.K |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| RL5_AZOVD |
52 | 20433 | 30.1 | ||
| 50S ribosomal protein L5 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rplE PE=3 SV=1 | |||||
| 6 samesets of RL5_AZOVD | |||||
| RL5_PSEA7 |
52 | 20393 | |||
| 50S ribosomal protein L5 OS=Pseudomonas aeruginosa (strain PA7) GN=rplE PE=3 SV=1 | |||||
| RL5_PSEA8 |
52 | 20379 | |||
| 50S ribosomal protein L5 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplE PE=3 SV=1 | |||||
| RL5_PSEAB |
52 | 20379 | |||
| 50S ribosomal protein L5 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplE PE=3 SV=1 | |||||
| RL5_PSEAE |
52 | 20379 | |||
| 50S ribosomal protein L5 OS=Pseudomonas aeruginosa GN=rplE PE=3 SV=1 | |||||
| RL5_PSEMY |
52 | 20437 | |||
| 50S ribosomal protein L5 OS=Pseudomonas mendocina (strain ymp) GN=rplE PE=3 SV=1 | |||||
| RL5_PSEU5 |
52 | 20384 | |||
| 50S ribosomal protein L5 OS=Pseudomonas stutzeri (strain A1501) GN=rplE PE=3 SV=1 | |||||
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