MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 179)

Page: 1 2 3 4 5 6 … 18 Next 

+1

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1121 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
6 EFTU_CYAP7 40 Elongation factor Tu OS=Cyanothece sp. (strain PCC 7424) GN=tuf PE=3 SV=1
5 EFTU_ANATD 50 Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1
3 EFTU_PSEFS 701 Elongation factor Tu OS=Pseudomonas fluorescens (strain SBW25) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1002 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1
4 EFTU_CARRP 145 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1

+2

Accession Score Description
1 ATPB_PSEPG 972 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1

-3

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPK 749 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
2 ATPA_RICAH 179 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
3 ATPA_VEREI 174 ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
ATPA_PSEPK 749 55489 57 (33) 24 (15) 0.99
ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
1 sameset of ATPA_PSEPK
ATPA_PSEP1 749 55458 57 (33) 24 (15) 0.99
ATP synthase subunit alpha OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=atpA PE=3 SV=1
ATPA_RICAH 179 56389 14 (11) 5 (4) 0.27
ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
7 samesets of ATPA_RICAH
ATPA_RICCN 179 56128 14 (11) 5 (4) 0.27
ATP synthase subunit alpha OS=Rickettsia conorii GN=atpA PE=3 SV=2
ATPA_RICFE 179 56172 14 (11) 5 (4) 0.27
ATP synthase subunit alpha OS=Rickettsia felis GN=atpA PE=3 SV=1
ATPA_RICPU 179 56160 14 (11) 5 (4) 0.27
ATP synthase subunit alpha OS=Rickettsia peacockii (strain Rustic) GN=atpA PE=3 SV=1
ATPA_RICRO 179 56195 14 (11) 5 (4) 0.27
ATP synthase subunit alpha OS=Rickettsia rickettsii (strain Iowa) GN=atpA PE=3 SV=2
ATPA_RICRS 179 56195 14 (11) 5 (4) 0.27
ATP synthase subunit alpha OS=Rickettsia rickettsii (strain Sheila Smith) GN=atpA PE=3 SV=1
ATPA_RICM5 179 56127 14 (11) 5 (4) 0.27
ATP synthase subunit alpha OS=Rickettsia massiliae (strain Mtu5) GN=atpA PE=3 SV=2
ATPA_RICB8 179 56616 14 (11) 5 (4) 0.27
ATP synthase subunit alpha OS=Rickettsia bellii (strain OSU 85-389) GN=atpA PE=3 SV=1
ATPA_VEREI 174 57757 12 (5) 5 (2) 0.17
ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1

-63 peptide matches (37 non-duplicate, 26 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 Peptide
240   517.3123 516.3050 516.3020 5.91 1 2 2.4 +5Score > 43 indicates identity
Score > 18 indicates homology		 X E.KNGAK.W
310 +1 602.3640 601.3567 601.3548 3.28 0 17 0.98 +1Score > 43 indicates identity
Score > 29 indicates homology		 X K.LSGGIR.T
311 +1 301.6857 601.3568 601.3548 3.49 0 25 0.89 +1Score > 43 indicates identity
Score > 37 indicates homology		 X K.LSGGIR.T
314   606.2905 605.2832 605.2809 3.79 0 16 0.12 +1Score > 40 indicates identity
Score > 19 indicates homology		 U X K.ATQTW.-
372 +1 318.1803 634.3460 634.3438 3.48 0 31 0.043 +1Score > 41 indicates identity
Score > 30 indicates homology		 U X E.LAQFR.E + Deamidated (NQ)
528 +1 354.1966 706.3786 706.3762 3.46 0 35 0.19 +1Score > 41 indicates identity X X K.QAVAYR.Q
529   707.3874 706.3801 706.3762 5.55 0 23 0.4 +1Score > 39 indicates identity
Score > 31 indicates homology		 X X K.QAVAYR.Q
582   365.7353 729.4560 729.4497 8.69 1 17 0.83 +1Score > 36 indicates identity
Score > 28 indicates homology		 X K.KLSGGIR.T
763 +1 408.2360 814.4574 814.4548 3.19 1 32 0.68 +4Score > 43 indicates identity X X X R.ELIIGDR.Q
769 +1 411.7281 821.4416 821.4395 2.58 0 32 0.23 +1Score > 40 indicates identity
Score > 38 indicates homology		 X R.TALAQYR.E
770   822.4496 821.4423 821.4395 3.40 0 36 0.07 +1Score > 40 indicates identity
Score > 37 indicates homology		 X R.TALAQYR.E
820   842.5526 841.5453 841.5385 8.09 0 47 0.0036 +1Score > 35 indicates identity U X X R.QISLLLR.R
821 +2 421.7801 841.5456 841.5385 8.48 0 41 0.013 +1Score > 35 indicates identity U X X R.QISLLLR.R
858 +2 427.7731 853.5316 853.5273 5.10 1 43 0.0053 +1Score > 33 indicates identity U X R.ILEVPVGK.E
874   859.5043 858.4970 858.4923 5.49 0 34 0.31 +1Score > 42 indicates identity U X R.STVANIVR.K
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_2_20250714115216.raw

Score > 42 indicates identity
875   430.2559 858.4972 858.4923 5.75 0 38 0.12 -1Score > 42 indicates identity U X R.STVANIVR.K
5.75 0 38 0.12 1 STVANLVR  
5.73 0 30 0.87 3 STVAQVVR  
18.9 1 23 4.1 4 EQLTVIR   + Deamidated (NQ)
18.9 1 23 4.1 4 EQLTVLR   + Deamidated (NQ)
5.77 0 19 11 6 ITASQALR  
5.75 0 17 15 7 ISGTVNIR  
5.73 0 16 20 8 LSGTQVVR  
5.73 0 16 21 9 STAVGVGIR  
5.77 1 16 21 10 SVAKDAIR  
949   448.2269 894.4392 894.4447 -6.10 0 35 0.088 +1Score > 41 indicates identity
Score > 37 indicates homology		 U X K.FTNGAVTGK.T + Deamidated (NQ)
952   897.5338 896.5265 896.5232 3.68 0 16 2.3 +4Score > 36 indicates identity
Score > 32 indicates homology		 U X K.VAPGVIWR.K
953 +2 449.2711 896.5276 896.5232 4.93 0 46 0.005 +1Score > 36 indicates identity U X K.VAPGVIWR.K
1463 +2 553.8154 1105.6162 1105.6019 13.0 1 43 0.00072 +1Score > 39 indicates identity
Score > 24 indicates homology		 U X R.GFLIDVEVSK.I
1663 +2 599.3306 1196.6466 1196.6401 5.47 0 64 0.00015 +1Score > 39 indicates identity U X X R.VVDALGNPIDGK.G
1711 +2 609.3122 1216.6098 1216.6048 4.18 0 69 0.00011 +1Score > 42 indicates identity U X R.IDNLDVSSQAR.N
1720 +1 611.3112 1220.6078 1220.6037 3.37 0 43 0.00091 +1Score > 42 indicates identity
Score > 25 indicates homology		 U X K.SVDQPVQTGYK.S
1861 +1 644.8575 1287.7004 1287.6856 11.5 0 43 0.025 +1Score > 40 indicates identity U X K.TAMAIDAIINQK.D
1869 +1 647.3052 1292.5958 1292.5885 5.72 1 102 3.9e-008 +1Score > 40 indicates identity U X K.GDFNDEIDAGLK.A
1947 +1 665.3380 1328.6614 1328.6572 3.19 1 94 2.6e-008 +1Score > 41 indicates identity
Score > 31 indicates homology		 U X K.GPLGNTQTDAVEK.V
1985   450.5759 1348.7059 1348.6987 5.33 1 14 0.81 +1Score > 40 indicates identity
Score > 26 indicates homology		 U X R.KSVDQPVQTGYK.S
2172   477.5848 1429.7326 1429.7273 3.66 1 26 0.12 +1Score > 41 indicates identity
Score > 29 indicates homology		 U X K.GRIDNLDVSSQAR.N
2173   715.8745 1429.7344 1429.7273 4.97 1 60 0.00015 +1Score > 41 indicates identity
Score > 34 indicates homology		 U X K.GRIDNLDVSSQAR.N
2175 +1 716.3793 1430.7440 1430.7365 5.25 1 81 4.6e-006 +1Score > 40 indicates identity U X R.NEGTVVSVSDGIVR.I
2408   777.3773 1552.7400 1552.7310 5.81 1 86 1.7e-006 +1Score > 41 indicates identity U X X X R.EAYPGDVFYLHSR.L
2410 +1 518.5886 1552.7440 1552.7310 8.33 1 71 4.9e-005 +1Score > 41 indicates identity U X X X R.EAYPGDVFYLHSR.L
2542   806.9408 1611.8670 1611.8409 16.2 1 11 0.92 +1Score > 39 indicates identity
Score > 23 indicates homology		 U X K.IGSFEQALIAFFNR.D
2543 +1 538.2965 1611.8677 1611.8409 16.6 1 78 7e-006 +1Score > 39 indicates identity U X K.IGSFEQALIAFFNR.D
2751   854.9291 1707.8436 1707.8315 7.10 1 81 3.3e-006 +1Score > 41 indicates identity
Score > 39 indicates homology		 U X R.DRGQDALIVYDDLSK.Q + Deamidated (NQ)
2752 +1 570.2894 1707.8464 1707.8315 8.69 1 46 0.0027 +1Score > 41 indicates identity
Score > 33 indicates homology		 U X R.DRGQDALIVYDDLSK.Q + Deamidated (NQ)
3371   701.0168 2100.0286 2100.0019 12.7 1 44 0.00024 +1Score > 39 indicates identity
Score > 21 indicates homology		 U X K.QYAPMSIADMALSLYAAER.G

+36 subsets and intersections (858 subset proteins in total)

+4

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 584 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
3 CH602_SORC5 80 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1
2 CH601_ECOK1 123 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1

+5

Accession Score Description
1 DLDH2_PSEPU 541 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4

+6

Accession Score Description
1 RL1_PSEE4 419 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1

+7

Accession Score Description
1 EFG1_PSEPK 337 Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1

+8

Accession Score Description
1 RS5_PSEE4 331 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1

+9

Accession Score Description
Family member distances as a dendrogram 1 RL4_PSE14 327 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1
2 RL4_PSEE4 318 50S ribosomal protein L4 OS=Pseudomonas entomophila (strain L48) GN=rplD PE=3 SV=1

+10

Accession Score Description
1 RL18_PSEP1 310 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1
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