MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 179)

Page: Previous 1 2 3 4 5 6 7 8 … 18 Next 

+21

Accession Score Description
1 RPOA_PSEPK 230 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

+22

Accession Score Description
1 OTCC_PSEPK 229 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3

+23

Accession Score Description
1 ADHP_ECOLI 207 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

+24

Accession Score Description
1 RL3_PSEE4 193 50S ribosomal protein L3 OS=Pseudomonas entomophila (strain L48) GN=rplC PE=3 SV=1

+25

Accession Score Description
1 PORF_PSEAE 190 Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1

+26

Accession Score Description
1 RL10_PSEPG 183 50S ribosomal protein L10 OS=Pseudomonas putida (strain GB-1) GN=rplJ PE=3 SV=1

+27

Accession Score Description
1 RS16_PSEP1 180 30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1

-28

Accession Score Description
1 SUCC_PSEPK 176 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1
Score Mass Matches Sequences emPAI
28.1 SUCC_PSEPK 176 41499 13 (6) 8 (4) 0.24
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1
3 samesets of SUCC_PSEPK
SUCC_PSEPW 176 41472 13 (6) 8 (4) 0.24
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain W619) GN=sucC PE=3 SV=1
SUCC_PSEP1 176 41499 13 (6) 8 (4) 0.24
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=sucC PE=3 SV=1
SUCC_PSEE4 176 41472 13 (6) 8 (4) 0.24
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas entomophila (strain L48) GN=sucC PE=3 SV=1

-13 peptide matches (8 non-duplicate, 5 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
416 +1 334.7274 667.4402 667.4381 3.20 0 24 0.16 +1Score > 28 indicates identity K.VPVVVR.L
609 +1 374.2436 746.4726 746.4691 4.81 0 30 0.11 +1Score > 33 indicates identity K.IFVGLAK.L
1212 +1 496.2753 990.5360 990.5287 7.43 0 35 0.01 +1Score > 41 indicates identity
Score > 28 indicates homology		 U K.AFAAQWLGK.N
1530 +1 567.8163 1133.6180 1133.6081 8.81 1 24 0.13 +1Score > 40 indicates identity
Score > 27 indicates homology		 K.ELYLGAVVDR.S
1860   430.1916 1287.5530 1287.5480 3.86 0 9 0.36 +1Score > 37 indicates identity
Score > 17 indicates homology		 U K.TFHDPSQDDAR.E
1992   676.3745 1350.7344 1350.7183 11.9 1 59 3.6e-005 +1Score > 38 indicates identity
Score > 27 indicates homology		 U K.QLFAEYGLPVSK.G
2443 +1 785.4326 1568.8506 1568.8311 12.5 0 84 4.3e-007 +1Score > 38 indicates identity
Score > 33 indicates homology		 U K.ATIDPLVGAQPFQGR.E
2817   868.4384 1734.8622 1734.8424 11.4 0 64 1.6e-005 +1Score > 40 indicates identity
Score > 29 indicates homology		 U K.NLVTYQTDANGQPVSK.I + Deamidated (NQ)

6 subsets and intersections (19 subset proteins in total)

Score Mass Subset of
SUCC_PSEPG 141 41413 28.1
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain GB-1) GN=sucC PE=3 SV=1
SUCC_PSE14 131 41494 28.1
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=sucC PE=3 SV=1
4 samesets of SUCC_PSE14
SUCC_PSEFS 131 41547
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas fluorescens (strain SBW25) GN=sucC PE=3 SV=1
SUCC_PSEF5 131 41599
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=sucC PE=3 SV=1
SUCC_PSESM 131 41472
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas syringae pv. tomato GN=sucC PE=3 SV=1
SUCC_PSEU2 131 41442
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas syringae pv. syringae (strain B728a) GN=sucC PE=3 SV=1
SUCC_PSEPF 99 41527 28.1
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas fluorescens (strain Pf0-1) GN=sucC PE=3 SV=1
SUCC_PSEA7 95 41772 28.1
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas aeruginosa (strain PA7) GN=sucC PE=3 SV=1
3 samesets of SUCC_PSEA7
SUCC_PSEAB 95 41830
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=sucC PE=3 SV=1
SUCC_PSEAE 95 41802
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas aeruginosa GN=sucC PE=3 SV=2
SUCC_PSEA8 95 41802
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas aeruginosa (strain LESB58) GN=sucC PE=3 SV=1
SUCC_VIBC3 64 41658 28.1
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) GN=sucC PE=3 SV=1
2 samesets of SUCC_VIBC3
SUCC_VIBCH 64 41658
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Vibrio cholerae GN=sucC PE=3 SV=1
SUCC_VIBCM 64 41658
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Vibrio cholerae serotype O1 (strain M66-2) GN=sucC PE=3 SV=1
SUCC_MARAV 59 41495 28.1
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Marinobacter aquaeolei (strain ATCC 700491 / DSM 11845 / VT8) GN=sucC PE=3 SV=1
4 samesets of SUCC_MARAV
SUCC_PSEU5 59 41761
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas stutzeri (strain A1501) GN=sucC PE=3 SV=1
SUCC_SACD2 59 41524
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=sucC PE=3 SV=1
SUCC_PSEMY 59 41759
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas mendocina (strain ymp) GN=sucC PE=3 SV=1
SUCC_TERTT 59 41703
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Teredinibacter turnerae (strain ATCC 39867 / T7901) GN=sucC PE=3 SV=1

+29

Accession Score Description
1 TIG_PSEPG 172 Trigger factor OS=Pseudomonas putida (strain GB-1) GN=tig PE=3 SV=1

+30

Accession Score Description
1 RL5_PSEE4 166 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1
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