MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 179)

Page: Previous 1 2 3 4 5 6 7 8 … 18 Next 

+21

Accession Score Description
1 RPOA_PSEPK 230 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

+22

Accession Score Description
1 OTCC_PSEPK 229 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3

+23

Accession Score Description
1 ADHP_ECOLI 207 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

+24

Accession Score Description
1 RL3_PSEE4 193 50S ribosomal protein L3 OS=Pseudomonas entomophila (strain L48) GN=rplC PE=3 SV=1

+25

Accession Score Description
1 PORF_PSEAE 190 Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1

+26

Accession Score Description
1 RL10_PSEPG 183 50S ribosomal protein L10 OS=Pseudomonas putida (strain GB-1) GN=rplJ PE=3 SV=1

-27

Accession Score Description
1 RS16_PSEP1 180 30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1
Score Mass Matches Sequences emPAI
27.1 RS16_PSEP1 180 9183 11 (7) 5 (3) 1.56
30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1
2 samesets of RS16_PSEP1
RS16_PSEPG 180 9183 11 (7) 5 (3) 1.56
30S ribosomal protein S16 OS=Pseudomonas putida (strain GB-1) GN=rpsP PE=3 SV=1
RS16_PSEPK 180 9183 11 (7) 5 (3) 1.56
30S ribosomal protein S16 OS=Pseudomonas putida (strain KT2440) GN=rpsP PE=3 SV=1

-11 peptide matches (7 non-duplicate, 4 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
432   336.2271 670.4396 670.4377 2.84 0 13 1.2 +5Score > 37 indicates identity
Score > 26 indicates homology		 R.VAQLLK.E
433   671.4498 670.4425 670.4377 7.13 0 19 0.17 +1Score > 37 indicates identity
Score > 24 indicates homology		 R.VAQLLK.E
826 +1 423.2286 844.4426 844.4403 2.83 1 50 0.013 +1Score > 43 indicates identity U K.LSVNQER.V
2143 +2 710.3969 1418.7792 1418.7558 16.5 1 59 4.1e-005 +1Score > 39 indicates identity
Score > 27 indicates homology		 U R.VGFFNPIAAGAEVK.L
2572   405.7283 1618.8841 1618.8580 16.1 1 6 0.98 +1Score > 38 indicates identity
Score > 18 indicates homology		 K.KRPFYHLTVTNSR.N + Deamidated (NQ)
2575   541.2725 1620.7957 1620.7896 3.74 1 37 0.017 +1Score > 41 indicates identity
Score > 31 indicates homology		 U R.VTYWLSQGAQPSER.V
2577 +1 811.4077 1620.8008 1620.7896 6.93 1 75 1e-005 +1Score > 41 indicates identity
Score > 38 indicates homology		 U R.VTYWLSQGAQPSER.V

3 subsets and intersections (6 subset proteins in total)

Score Mass Subset of
RS16_PSEE4 114 9199 27.1
30S ribosomal protein S16 OS=Pseudomonas entomophila (strain L48) GN=rpsP PE=3 SV=1
RS16_PSEPW 108 9155 27.1
30S ribosomal protein S16 OS=Pseudomonas putida (strain W619) GN=rpsP PE=3 SV=1
RS16_PSEF5 50 9230 27.1
30S ribosomal protein S16 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsP PE=3 SV=1
3 samesets of RS16_PSEF5
RS16_PSEPF 50 9230
30S ribosomal protein S16 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsP PE=3 SV=1
RS16_PSEU5 50 9195
30S ribosomal protein S16 OS=Pseudomonas stutzeri (strain A1501) GN=rpsP PE=3 SV=1
RS16_PSEFS 50 9202
30S ribosomal protein S16 OS=Pseudomonas fluorescens (strain SBW25) GN=rpsP PE=3 SV=1

+28

Accession Score Description
1 SUCC_PSEPK 176 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1

+29

Accession Score Description
1 TIG_PSEPG 172 Trigger factor OS=Pseudomonas putida (strain GB-1) GN=tig PE=3 SV=1

+30

Accession Score Description
1 RL5_PSEE4 166 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1
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