MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 179)

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+21

Accession Score Description
1 RPOA_PSEPK 230 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

+22

Accession Score Description
1 OTCC_PSEPK 229 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3

+23

Accession Score Description
1 ADHP_ECOLI 207 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

+24

Accession Score Description
1 RL3_PSEE4 193 50S ribosomal protein L3 OS=Pseudomonas entomophila (strain L48) GN=rplC PE=3 SV=1

+25

Accession Score Description
1 PORF_PSEAE 190 Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1

-26

Accession Score Description
1 RL10_PSEPG 183 50S ribosomal protein L10 OS=Pseudomonas putida (strain GB-1) GN=rplJ PE=3 SV=1
Score Mass Matches Sequences emPAI
26.1 RL10_PSEPG 183 17676 7 (4) 4 (2) 0.28
50S ribosomal protein L10 OS=Pseudomonas putida (strain GB-1) GN=rplJ PE=3 SV=1
3 samesets of RL10_PSEPG
RL10_PSEPK 183 17676 7 (4) 4 (2) 0.28
50S ribosomal protein L10 OS=Pseudomonas putida (strain KT2440) GN=rplJ PE=3 SV=1
RL10_PSEE4 183 17602 6 (4) 3 (2) 0.28
50S ribosomal protein L10 OS=Pseudomonas entomophila (strain L48) GN=rplJ PE=3 SV=1
RL10_PSEPW 183 17648 6 (4) 3 (2) 0.28
50S ribosomal protein L10 OS=Pseudomonas putida (strain W619) GN=rplJ PE=3 SV=1

-7 peptide matches (4 non-duplicate, 3 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
384   322.7095 643.4044 643.4017 4.29 0 16 0.85 +6Score > 42 indicates identity
Score > 28 indicates homology		 R.TLAAIR.D
719   397.2150 792.4154 792.4130 3.10 1 34 0.093 +1Score > 42 indicates identity
Score > 36 indicates homology		 R.EAGVYVR.V
1397 +2 536.3147 1070.6148 1070.6084 6.02 0 83 1.4e-006 +1Score > 40 indicates identity
Score > 37 indicates homology		 U K.VALSAVVADAR.G
2754 +1 570.2999 1707.8779 1707.8692 5.05 1 64 4.8e-006 +1Score > 40 indicates identity
Score > 23 indicates homology		 U K.GPTLIAFSNEHPGAAAR.L

1 subset or intersection (7 subset proteins in total)

Score Mass Subset of
RL10_AZOVD 97 17534 26.1
50S ribosomal protein L10 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rplJ PE=3 SV=1
6 samesets of RL10_AZOVD
RL10_PSEA7 97 17623
50S ribosomal protein L10 OS=Pseudomonas aeruginosa (strain PA7) GN=rplJ PE=3 SV=1
RL10_PSEA8 97 17623
50S ribosomal protein L10 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplJ PE=3 SV=1
RL10_PSEAB 97 17623
50S ribosomal protein L10 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplJ PE=3 SV=1
RL10_PSEAE 97 17623
50S ribosomal protein L10 OS=Pseudomonas aeruginosa GN=rplJ PE=3 SV=1
RL10_PSEMY 97 17574
50S ribosomal protein L10 OS=Pseudomonas mendocina (strain ymp) GN=rplJ PE=3 SV=1
RL10_PSEU5 97 17514
50S ribosomal protein L10 OS=Pseudomonas stutzeri (strain A1501) GN=rplJ PE=3 SV=1

+27

Accession Score Description
1 RS16_PSEP1 180 30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1

+28

Accession Score Description
1 SUCC_PSEPK 176 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1

+29

Accession Score Description
1 TIG_PSEPG 172 Trigger factor OS=Pseudomonas putida (strain GB-1) GN=tig PE=3 SV=1

+30

Accession Score Description
1 RL5_PSEE4 166 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1
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