MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita2 sp |
| MS data file | : | PRT1270_T-BRSC_2_20250714115216.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:39:15 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,778 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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21| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOA_PSEPK | 230 | DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1 |
22| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | OTCC_PSEPK | 229 | Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3 |
23| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ADHP_ECOLI | 207 | Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1 |
24| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL3_PSEE4 | 193 | 50S ribosomal protein L3 OS=Pseudomonas entomophila (strain L48) GN=rplC PE=3 SV=1 |
25| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PORF_PSEAE | 190 | Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 25.1 | PORF_PSEAE |
190 | 37844 | 17 (7) | 4 (2) | 0.13 | |
| Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1 | |||||||
17 peptide matches (6 non-duplicate, 11 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 422 |
2 |
336.1564 | 670.2982 | 670.2962 | 2.98 | 0 | 20 | 0.87 | 1Score > 37 indicates identity Score > 32 indicates homology |
K.FDFDK.S | |
| 424 |
1 |
671.3060 | 670.2987 | 670.2962 | 3.69 | 0 | 27 | 0.32 | 1Score > 37 indicates identity Score > 35 indicates homology |
K.FDFDK.S | |
| 508 |
1 |
702.4052 | 701.3979 | 701.3959 | 2.81 | 0 | 42 | 0.07 | 1Score > 43 indicates identity |
U | R.VQLDVK.F + Deamidated (NQ) |
| 509 |
1 |
351.7063 | 701.3980 | 701.3959 | 2.99 | 0 | 34 | 0.3 | 1Score > 43 indicates identity Score > 41 indicates homology |
U | R.VQLDVK.F + Deamidated (NQ) |
| 808 |
2 |
419.7118 | 837.4090 | 837.4055 | 4.29 | 0 | 37 | 0.019 | 1Score > 40 indicates identity Score > 33 indicates homology |
U | K.NLADFMK.Q |
| 2001 |
4 |
452.2394 | 1353.6964 | 1353.6816 | 10.9 | 1 | 76 | 3.4e-006 | 1Score > 40 indicates identity Score > 34 indicates homology |
U | R.VQLDVKFDFDK.S + Deamidated (NQ) |
26| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL10_PSEPG | 183 | 50S ribosomal protein L10 OS=Pseudomonas putida (strain GB-1) GN=rplJ PE=3 SV=1 |
27| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS16_PSEP1 | 180 | 30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1 |
28| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SUCC_PSEPK | 176 | Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1 |
29| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TIG_PSEPG | 172 | Trigger factor OS=Pseudomonas putida (strain GB-1) GN=tig PE=3 SV=1 |
30| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL5_PSEE4 | 166 | 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1 |
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