MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 179)


Page: Previous 1 2 3 4 5 6 7 8  18 Next 

+21

Accession Score Description
1 RPOA_PSEPK 230 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

+22

Accession Score Description
1 OTCC_PSEPK 229 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3

+23

Accession Score Description
1 ADHP_ECOLI 207 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

-24

Accession Score Description
1 RL3_PSEE4 193 50S ribosomal protein L3 OS=Pseudomonas entomophila (strain L48) GN=rplC PE=3 SV=1
Score Mass Matches Sequences emPAI
24.1 RL3_PSEE4 193 22600 13 (7) 7 (4) 0.80
50S ribosomal protein L3 OS=Pseudomonas entomophila (strain L48) GN=rplC PE=3 SV=1
4 samesets of RL3_PSEE4
RL3_PSEP1 193 22703 13 (7) 7 (4) 0.80
50S ribosomal protein L3 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplC PE=3 SV=1
RL3_PSEPG 193 22703 13 (7) 7 (4) 0.80
50S ribosomal protein L3 OS=Pseudomonas putida (strain GB-1) GN=rplC PE=3 SV=1
RL3_PSEPK 193 22703 13 (7) 7 (4) 0.80
50S ribosomal protein L3 OS=Pseudomonas putida (strain KT2440) GN=rplC PE=3 SV=2
RL3_PSEPW 193 22663 13 (7) 7 (4) 0.80
50S ribosomal protein L3 OS=Pseudomonas putida (strain W619) GN=rplC PE=3 SV=1

-13 peptide matches (10 non-duplicate, 3 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
485   347.2015 692.3884 692.3857 3.93 0 14 1.6 +2Score > 39 indicates identity
Score > 28 indicates homology
K.GFAGTIK.R
717   397.2050 792.3954 792.3919 4.51 1 12 1.2 +2Score > 42 indicates identity
Score > 25 indicates homology
R.GVWEFR.L
1135 +1 479.7721 957.5296 957.5244 5.53 1 40 0.055 +1Score > 41 indicates identity
Score > 40 indicates homology
R.AVQVTVGER.R
1451 +1 367.5343 1099.5811 1099.5774 3.30 0 30 0.22 +1Score > 41 indicates identity
Score > 36 indicates homology
U R.VTAAQAGHFAK.A
1452   551.2951 1100.5756 1100.5614 12.9 0 39 0.0097 +1Score > 41 indicates identity
Score > 32 indicates homology
U R.VTAAQAGHFAK.A + Deamidated (NQ)
1511 +1 565.3362 1128.6578 1128.6503 6.70 1 51 0.0028 +1Score > 38 indicates identity U R.VTVQSLEVVR.V
2630   825.4730 1648.9314 1648.9261 3.25 0 75 5.4e-006 +1Score > 34 indicates identity U K.GAVPGATGGDVVVRPAVK.A
2631   550.6519 1648.9339 1648.9261 4.72 0 47 0.0032 +1Score > 34 indicates identity U K.GAVPGATGGDVVVRPAVK.A
2844   588.6498 1762.9276 1762.9101 9.89 1 24 0.032 +1Score > 39 indicates identity
Score > 21 indicates homology
U K.AELFTAGQLVDVTGQSK.G
2845   882.4714 1762.9282 1762.9101 10.3 1 105 1.9e-009 +1Score > 39 indicates identity
Score > 30 indicates homology
U K.AELFTAGQLVDVTGQSK.G

3 subsets and intersections (23 subset proteins in total)

Score Mass Subset of
RL3_PSEF5 66 22646 24.1
50S ribosomal protein L3 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplC PE=3 SV=1
4 samesets of RL3_PSEF5
RL3_PSEFS 66 22664
50S ribosomal protein L3 OS=Pseudomonas fluorescens (strain SBW25) GN=rplC PE=3 SV=1
RL3_PSEPF 66 22603
50S ribosomal protein L3 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplC PE=3 SV=1
RL3_PSESM 66 22707
50S ribosomal protein L3 OS=Pseudomonas syringae pv. tomato GN=rplC PE=3 SV=1
RL3_PSEU2 66 22693
50S ribosomal protein L3 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplC PE=3 SV=1
RL3_ACTP2 61 22369 24.1
50S ribosomal protein L3 OS=Actinobacillus pleuropneumoniae serotype 5b (strain L20) GN=rplC PE=3 SV=1
16 samesets of RL3_ACTP2
RL3_PSEMY 61 22536
50S ribosomal protein L3 OS=Pseudomonas mendocina (strain ymp) GN=rplC PE=3 SV=1
RL3_ACTP7 61 22369
50S ribosomal protein L3 OS=Actinobacillus pleuropneumoniae serotype 7 (strain AP76) GN=rplC PE=3 SV=1
RL3_ACTPJ 61 22369
50S ribosomal protein L3 OS=Actinobacillus pleuropneumoniae serotype 3 (strain JL03) GN=rplC PE=3 SV=1
RL3_ACTSZ 61 22312
50S ribosomal protein L3 OS=Actinobacillus succinogenes (strain ATCC 55618 / 130Z) GN=rplC PE=3 SV=1
RL3_AGGAC 61 12929
50S ribosomal protein L3 (Fragment) OS=Aggregatibacter actinomycetemcomitans GN=rplC PE=3 SV=1
RL3_BUCA5 61 22903
50S ribosomal protein L3 OS=Buchnera aphidicola subsp. Acyrthosiphon pisum (strain 5A) GN=rplC PE=3 SV=1
RL3_BUCAI 61 22884
50S ribosomal protein L3 OS=Buchnera aphidicola subsp. Acyrthosiphon pisum GN=rplC PE=3 SV=1
RL3_BUCAT 61 22903
50S ribosomal protein L3 OS=Buchnera aphidicola subsp. Acyrthosiphon pisum (strain Tuc7) GN=rplC PE=3 SV=1
RL3_HAEDU 61 22295
50S ribosomal protein L3 OS=Haemophilus ducreyi GN=rplC PE=3 SV=1
RL3_HAEI8 61 22350
50S ribosomal protein L3 OS=Haemophilus influenzae (strain 86-028NP) GN=rplC PE=3 SV=1
RL3_HAEIG 61 22349
50S ribosomal protein L3 OS=Haemophilus influenzae (strain PittGG) GN=rplC PE=3 SV=1
RL3_HAEIN 61 22350
50S ribosomal protein L3 OS=Haemophilus influenzae GN=rplC PE=3 SV=1
RL3_HAEPS 61 22385
50S ribosomal protein L3 OS=Haemophilus parasuis serovar 5 (strain SH0165) GN=rplC PE=3 SV=1
RL3_MANSM 61 22326
50S ribosomal protein L3 OS=Mannheimia succiniciproducens (strain MBEL55E) GN=rplC PE=3 SV=1
RL3_PASMU 61 22369
50S ribosomal protein L3 OS=Pasteurella multocida GN=rplC PE=3 SV=1
RL3_TOLAT 61 22710
50S ribosomal protein L3 OS=Tolumonas auensis (strain DSM 9187 / TA4) GN=rplC PE=3 SV=1
RL3_PSE14 39 22707 24.1
50S ribosomal protein L3 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplC PE=3 SV=1

+25

Accession Score Description
1 PORF_PSEAE 190 Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1

+26

Accession Score Description
1 RL10_PSEPG 183 50S ribosomal protein L10 OS=Pseudomonas putida (strain GB-1) GN=rplJ PE=3 SV=1

+27

Accession Score Description
1 RS16_PSEP1 180 30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1

+28

Accession Score Description
1 SUCC_PSEPK 176 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1

+29

Accession Score Description
1 TIG_PSEPG 172 Trigger factor OS=Pseudomonas putida (strain GB-1) GN=tig PE=3 SV=1

+30

Accession Score Description
1 RL5_PSEE4 166 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1
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