MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 179)


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+21

Accession Score Description
1 RPOA_PSEPK 230 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

-22

Accession Score Description
1 OTCC_PSEPK 229 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3
Score Mass Matches Sequences emPAI
22.1 OTCC_PSEPK 229 38115 27 (18) 10 (7) 0.91
Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3

+27 peptide matches (16 non-duplicate, 11 duplicate)

4 subsets and intersections (27 subset proteins in total)

Score Mass Subset of
OTCC_PSEAE 75 38255 22.1
Ornithine carbamoyltransferase, catabolic OS=Pseudomonas aeruginosa GN=arcB PE=1 SV=3
1 sameset of OTCC_PSEAE
OTCC_PSEME 75 38189
Ornithine carbamoyltransferase, catabolic OS=Pseudomonas mendocina GN=arcB PE=3 SV=3
OTCC_CHRVO 45 38025 22.1
Ornithine carbamoyltransferase, catabolic OS=Chromobacterium violaceum GN=arcB PE=3 SV=1
OTCC_HAEIN 41 37908 22.1
Ornithine carbamoyltransferase, catabolic OS=Haemophilus influenzae GN=arcB PE=3 SV=1
3 samesets of OTCC_HAEIN
OTC_HAEIG 41 37881
Ornithine carbamoyltransferase OS=Haemophilus influenzae (strain PittGG) GN=arcB PE=3 SV=1
OTCC_MYCCC 41 35232
Ornithine carbamoyltransferase, catabolic OS=Mycoplasma capricolum subsp. capripneumoniae GN=arcB PE=3 SV=1
THI22_YEAST 41 64521
Thiamine biosynthesis protein THI22 OS=Saccharomyces cerevisiae GN=THI22 PE=2 SV=1
AMN1_PICAN 35 0 22.1
description
19 samesets of AMN1_PICAN
SYA_IGNH4 35 0
description
CIPKB_ORYSJ 35 0
description
YHC3_SCHPO 35 0
description
UNC53_CAEEL 35 0
description
DNAE2_RALPJ 35 0
description
CHDM_DROME 35 0
description
FAS_RAT 35 0
description
GB_MCMVS 35 0
description
MURI_LACAC 35 0
description
OTC_CLOAB 35 0
description
THEG_HUMAN 35 0
description
BOP1_DICDI 35 0
description
DOP1_EMENI 35 0
description
IML1_NEUCR 35 0
description
DNAE2_RALSO 35 0
description
IMPA3_MOUSE 35 0
description
RL3_SPHWW 35 0
description
RS4_DROME 35 0
description
RL32_CARHZ 35 0
description

+23

Accession Score Description
1 ADHP_ECOLI 207 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

+24

Accession Score Description
1 RL3_PSEE4 193 50S ribosomal protein L3 OS=Pseudomonas entomophila (strain L48) GN=rplC PE=3 SV=1

+25

Accession Score Description
1 PORF_PSEAE 190 Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1

+26

Accession Score Description
1 RL10_PSEPG 183 50S ribosomal protein L10 OS=Pseudomonas putida (strain GB-1) GN=rplJ PE=3 SV=1

+27

Accession Score Description
1 RS16_PSEP1 180 30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1

+28

Accession Score Description
1 SUCC_PSEPK 176 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1

+29

Accession Score Description
1 TIG_PSEPG 172 Trigger factor OS=Pseudomonas putida (strain GB-1) GN=tig PE=3 SV=1

+30

Accession Score Description
1 RL5_PSEE4 166 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1
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