MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 179)


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+21

Accession Score Description
1 RPOA_PSEPK 230 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

-22

Accession Score Description
1 OTCC_PSEPK 229 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3
Score Mass Matches Sequences emPAI
22.1 OTCC_PSEPK 229 38115 27 (18) 10 (7) 0.91
Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3

-27 peptide matches (16 non-duplicate, 11 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
218   499.3257 498.3184 498.3166 3.74 0 35 0.042 +1Score > 34 indicates identity U R.IAAPK.A
482   345.6848 689.3550 689.3530 2.91 0 25 0.28 +1Score > 43 indicates identity
Score > 32 indicates homology
K.LGMDVR.I
896 +1 436.2323 870.4500 870.4460 4.63 0 28 0.13 +1Score > 39 indicates identity
Score > 32 indicates homology
M.AFNIHNR.N
916   440.2535 878.4924 878.4861 7.17 0 34 0.14 +5Score > 38 indicates identity U R.YLLDLSR.D
918   879.5005 878.4932 878.4861 8.06 0 41 0.024 +1Score > 37 indicates identity U R.YLLDLSR.D
1005 +1 458.7545 915.4944 915.4913 3.44 1 41 0.022 +1Score > 43 indicates identity
Score > 37 indicates homology
U R.ITLTEDPK.A
1283 +2 1015.6138 1014.6065 1014.5961 10.3 0 13 0.71 +1Score > 37 indicates identity
Score > 24 indicates homology
U K.AILVSTLADL.-
1285   508.3112 1014.6078 1014.5961 11.6 0 11 0.82 +1Score > 37 indicates identity
Score > 23 indicates homology
U K.AILVSTLADL.-
1479 +1 559.8199 1117.6252 1117.6131 10.8 1 51 0.0012 +1Score > 39 indicates identity
Score > 34 indicates homology
U K.GNNIALIFEK.T
1682   402.2050 1203.5932 1203.5884 3.97 1 43 0.00051 +1Score > 42 indicates identity
Score > 23 indicates homology
U K.YTGTEQQHLK.G
1683   602.8041 1203.5936 1203.5884 4.37 1 45 0.0027 +1Score > 42 indicates identity
Score > 32 indicates homology
U K.YTGTEQQHLK.G
1923   660.8563 1319.6980 1319.6946 2.63 1 39 0.0019 +1Score > 41 indicates identity
Score > 24 indicates homology
U R.NLLSLEHHTTR.E
1924 +1 330.9319 1319.6985 1319.6946 2.97 1 44 0.0035 +1Score > 41 indicates identity
Score > 32 indicates homology
U R.NLLSLEHHTTR.E
1926 +2 440.9074 1319.7004 1319.6946 4.39 1 38 0.0025 +1Score > 40 indicates identity
Score > 24 indicates homology
U R.NLLSLEHHTTR.E
1987 +2 450.9001 1349.6785 1349.6728 4.21 1 44 0.001 +1Score > 41 indicates identity
Score > 26 indicates homology
U K.ALWPHDDLVER.C
1988 +1 675.8475 1349.6804 1349.6728 5.67 1 38 0.0038 +1Score > 41 indicates identity
Score > 26 indicates homology
U K.ALWPHDDLVER.C

4 subsets and intersections (27 subset proteins in total)

Score Mass Subset of
OTCC_PSEAE 75 38255 22.1
Ornithine carbamoyltransferase, catabolic OS=Pseudomonas aeruginosa GN=arcB PE=1 SV=3
1 sameset of OTCC_PSEAE
OTCC_PSEME 75 38189
Ornithine carbamoyltransferase, catabolic OS=Pseudomonas mendocina GN=arcB PE=3 SV=3
OTCC_CHRVO 45 38025 22.1
Ornithine carbamoyltransferase, catabolic OS=Chromobacterium violaceum GN=arcB PE=3 SV=1
OTCC_HAEIN 41 37908 22.1
Ornithine carbamoyltransferase, catabolic OS=Haemophilus influenzae GN=arcB PE=3 SV=1
3 samesets of OTCC_HAEIN
OTC_HAEIG 41 37881
Ornithine carbamoyltransferase OS=Haemophilus influenzae (strain PittGG) GN=arcB PE=3 SV=1
OTCC_MYCCC 41 35232
Ornithine carbamoyltransferase, catabolic OS=Mycoplasma capricolum subsp. capripneumoniae GN=arcB PE=3 SV=1
THI22_YEAST 41 64521
Thiamine biosynthesis protein THI22 OS=Saccharomyces cerevisiae GN=THI22 PE=2 SV=1
AMN1_PICAN 35 0 22.1
description
19 samesets of AMN1_PICAN
SYA_IGNH4 35 0
description
CIPKB_ORYSJ 35 0
description
YHC3_SCHPO 35 0
description
UNC53_CAEEL 35 0
description
DNAE2_RALPJ 35 0
description
CHDM_DROME 35 0
description
FAS_RAT 35 0
description
GB_MCMVS 35 0
description
MURI_LACAC 35 0
description
OTC_CLOAB 35 0
description
THEG_HUMAN 35 0
description
BOP1_DICDI 35 0
description
DOP1_EMENI 35 0
description
IML1_NEUCR 35 0
description
DNAE2_RALSO 35 0
description
IMPA3_MOUSE 35 0
description
RL3_SPHWW 35 0
description
RS4_DROME 35 0
description
RL32_CARHZ 35 0
description

+23

Accession Score Description
1 ADHP_ECOLI 207 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

+24

Accession Score Description
1 RL3_PSEE4 193 50S ribosomal protein L3 OS=Pseudomonas entomophila (strain L48) GN=rplC PE=3 SV=1

+25

Accession Score Description
1 PORF_PSEAE 190 Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1

+26

Accession Score Description
1 RL10_PSEPG 183 50S ribosomal protein L10 OS=Pseudomonas putida (strain GB-1) GN=rplJ PE=3 SV=1

+27

Accession Score Description
1 RS16_PSEP1 180 30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1

+28

Accession Score Description
1 SUCC_PSEPK 176 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1

+29

Accession Score Description
1 TIG_PSEPG 172 Trigger factor OS=Pseudomonas putida (strain GB-1) GN=tig PE=3 SV=1

+30

Accession Score Description
1 RL5_PSEE4 166 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1
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