MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 179)

Page: Previous 1 2 3 4 5 6 7 8 … 18 Next 

-21

Accession Score Description
1 RPOA_PSEPK 230 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1
Score Mass Matches Sequences emPAI
21.1 RPOA_PSEPK 230 36721 16 (10) 9 (6) 0.44
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1
2 samesets of RPOA_PSEPK
RPOA_PSEP1 230 36721 16 (10) 9 (6) 0.44
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoA PE=3 SV=1
RPOA_PSEE4 230 36721 16 (10) 9 (6) 0.44
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas entomophila (strain L48) GN=rpoA PE=3 SV=1

-16 peptide matches (9 non-duplicate, 7 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
358   315.1856 628.3566 628.3544 3.55 0 15 1.2 +3Score > 37 indicates identity
Score > 28 indicates homology		 K.TPNLGK.K
1163 +1 485.7843 969.5540 969.5495 4.72 1 39 0.061 +1Score > 39 indicates identity U K.ITLEPLER.G
1323 +1 517.7875 1033.5604 1033.5556 4.67 1 44 0.0039 +1Score > 41 indicates identity
Score > 33 indicates homology		 U R.IAYVVENAR.V
1543 +1 570.8104 1139.6062 1139.5975 7.69 0 39 0.0042 +1Score > 39 indicates identity
Score > 27 indicates homology		 U R.LDNWPPASLK.K
1545 +1 381.5412 1141.6018 1141.5992 2.22 0 34 0.01 +1Score > 40 indicates identity
Score > 27 indicates homology		 U R.GFGHTLGNALR.R
1754 +1 616.8415 1231.6684 1231.6561 10.0 0 57 0.00026 +1Score > 40 indicates identity
Score > 33 indicates homology		 U R.LQLDASFSPVR.R
1793 +1 625.8492 1249.6838 1249.6779 4.76 0 56 8.4e-005 +1Score > 39 indicates identity
Score > 28 indicates homology		 U R.HIDVQVVSPTR.A
2177   717.8654 1433.7162 1433.7337 -12.2 1 10 2.7 +10Score > 42 indicates identity
Score > 27 indicates homology		 U -.MQISVNEFLTPR.H
2441 +1 784.4194 1566.8242 1566.8042 12.8 1 64 6.5e-006 +1Score > 39 indicates identity
Score > 25 indicates homology		 U K.AENIYYIGDLIQR.T

7 subsets and intersections (186 subset proteins in total)

Score Mass Subset of
RPOA_PSE14 189 36765 21.1
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpoA PE=3 SV=1
5 samesets of RPOA_PSE14
RPOA_PSEPF 189 36723
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpoA PE=3 SV=1
RPOA_PSESM 189 36765
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas syringae pv. tomato GN=rpoA PE=3 SV=1
RPOA_PSEU2 189 36765
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpoA PE=3 SV=1
RPOA_PSEF5 189 36721
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpoA PE=3 SV=1
RPOA_PSEPU 189 36695
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida GN=rpoA PE=3 SV=1
RPOA_PSEA7 168 36741 21.1
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas aeruginosa (strain PA7) GN=rpoA PE=3 SV=1
3 samesets of RPOA_PSEA7
RPOA_PSEMY 168 36661
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas mendocina (strain ymp) GN=rpoA PE=3 SV=1
RPOA_PSEAB 168 36741
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rpoA PE=3 SV=1
RPOA_PSEAE 168 36741
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas aeruginosa GN=rpoA PE=3 SV=2
RPOA_PSEU5 166 36620 21.1
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas stutzeri (strain A1501) GN=rpoA PE=3 SV=1
RPOA_SACD2 156 36391 21.1
DNA-directed RNA polymerase subunit alpha OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=rpoA PE=3 SV=1
RPOA_AZOSB 113 35767 21.1
DNA-directed RNA polymerase subunit alpha OS=Azoarcus sp. (strain BH72) GN=rpoA PE=3 SV=1
5 samesets of RPOA_AZOSB
RPOA_CELJU 113 36326
DNA-directed RNA polymerase subunit alpha OS=Cellvibrio japonicus (strain Ueda107) GN=rpoA PE=3 SV=1
RPOA_CHRSD 113 37113
DNA-directed RNA polymerase subunit alpha OS=Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768) GN=rpoA PE=3 SV=1
RPOA_THISH 113 36890
DNA-directed RNA polymerase subunit alpha OS=Thioalkalivibrio sp. (strain HL-EbGR7) GN=rpoA PE=3 SV=1
RPOA_AZOSE 113 35666
DNA-directed RNA polymerase subunit alpha OS=Azoarcus sp. (strain EbN1) GN=rpoA PE=3 SV=1
RPOA_LEPCP 113 36343
DNA-directed RNA polymerase subunit alpha OS=Leptothrix cholodnii (strain ATCC 51168 / LMG 8142 / SP-6) GN=rpoA PE=3 SV=1
RPOA_ACIAC 105 36282 21.1
DNA-directed RNA polymerase subunit alpha OS=Acidovorax avenae subsp. citrulli (strain AAC00-1) GN=rpoA PE=3 SV=1
+52 samesets of RPOA_ACIAC
RPOA1_PSYIN 34 36427 21.1
DNA-directed RNA polymerase subunit alpha 1 OS=Psychromonas ingrahamii (strain 37) GN=rpoA1 PE=3 SV=1
+114 samesets of RPOA1_PSYIN

+22

Accession Score Description
1 OTCC_PSEPK 229 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3

+23

Accession Score Description
1 ADHP_ECOLI 207 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

+24

Accession Score Description
1 RL3_PSEE4 193 50S ribosomal protein L3 OS=Pseudomonas entomophila (strain L48) GN=rplC PE=3 SV=1

+25

Accession Score Description
1 PORF_PSEAE 190 Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1

+26

Accession Score Description
1 RL10_PSEPG 183 50S ribosomal protein L10 OS=Pseudomonas putida (strain GB-1) GN=rplJ PE=3 SV=1

+27

Accession Score Description
1 RS16_PSEP1 180 30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1

+28

Accession Score Description
1 SUCC_PSEPK 176 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1

+29

Accession Score Description
1 TIG_PSEPG 172 Trigger factor OS=Pseudomonas putida (strain GB-1) GN=tig PE=3 SV=1

+30

Accession Score Description
1 RL5_PSEE4 166 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1
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