MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita2 sp |
| MS data file | : | PRT1270_T-BRSC_2_20250714115216.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:39:15 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,778 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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11| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL11_PSEP1 | 307 | 50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1 |
12| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PORF_PSESY | 300 | Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1 |
13| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DBHB_PSEAE | 292 | DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3 |
14| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOC_PSEPG | 283 | DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain GB-1) GN=rpoC PE=3 SV=1 | |
| 3 | RPOC_SYNAS | 112 | DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1 | |
| 4 | FTSZ_PSEPK | 96 | Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3 | |
| 2 | RPOB_PSEE4 | 207 | DNA-directed RNA polymerase subunit beta OS=Pseudomonas entomophila (strain L48) GN=rpoB PE=3 SV=1 | |
15| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODO2_PSEAE | 268 | Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1 |
16| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DHSA_ECOLI | 267 | Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1 |
17| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ARCA_PSEPK | 262 | Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1 |
18| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ACON2_PSEAE | 261 | Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1 |
19| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HTPG_PSEPK | 255 | Chaperone protein htpG OS=Pseudomonas putida (strain KT2440) GN=htpG PE=3 SV=1 |
20| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL2_PSEP1 | 235 | 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 20.1 | RL2_PSEP1 |
235 | 29822 | 17 (7) | 10 (4) | 0.35 | |
| 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1 | |||||||
| 4 samesets of RL2_PSEP1 | |||||||
| RL2_PSEPK |
235 | 29822 | 17 (7) | 10 (4) | 0.35 | ||
| 50S ribosomal protein L2 OS=Pseudomonas putida (strain KT2440) GN=rplB PE=3 SV=1 | |||||||
| RL2_PSEPW |
235 | 29822 | 17 (7) | 10 (4) | 0.35 | ||
| 50S ribosomal protein L2 OS=Pseudomonas putida (strain W619) GN=rplB PE=3 SV=1 | |||||||
| RL2_PSEE4 |
235 | 29808 | 14 (7) | 9 (4) | 0.35 | ||
| 50S ribosomal protein L2 OS=Pseudomonas entomophila (strain L48) GN=rplB PE=3 SV=1 | |||||||
| RL2_PSEPG |
235 | 29794 | 12 (7) | 8 (4) | 0.35 | ||
| 50S ribosomal protein L2 OS=Pseudomonas putida (strain GB-1) GN=rplB PE=3 SV=1 | |||||||
17 peptide matches (12 non-duplicate, 5 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 389 |
1 |
325.1882 | 648.3618 | 648.3595 | 3.60 | 0 | 22 | 0.087 | 1Score > 40 indicates identity Score > 24 indicates homology |
R.LVDFR.R | |
| 523 |
352.7217 | 703.4288 | 703.4268 | 2.86 | 0 | 28 | 0.12 | 1Score > 35 indicates identity Score > 31 indicates homology |
R.YIIAPK.G | ||
| 698 |
392.7447 | 783.4748 | 783.4715 | 4.24 | 0 | 25 | 0.15 | 1Score > 36 indicates identity Score > 29 indicates homology |
R.GVRPTVR.G | ||
| 868 |
429.7434 | 857.4722 | 857.4719 | 0.41 | 0 | 47 | 0.019 | 1Score > 43 indicates identity |
U | K.AGNSLQLR.N | |
| 977 |
453.7192 | 905.4238 | 905.4243 | -0.47 | 1 | 38 | 0.064 | 1Score > 42 indicates identity Score > 39 indicates homology |
R.IEYDPNR.T | ||
| 1068 |
1 |
468.7631 | 935.5116 | 935.5076 | 4.30 | 1 | 27 | 0.093 | 1Score > 40 indicates identity Score > 29 indicates homology |
U | R.EGVYVTLR.L |
| 1348 |
1 |
522.7973 | 1043.5800 | 1043.5723 | 7.40 | 0 | 64 | 0.00027 | 1Score > 41 indicates identity |
U | R.SAGASAQLIAR.E |
| 1795 |
626.8312 | 1251.6478 | 1251.6401 | 6.22 | 0 | 42 | 0.0062 | 1Score > 40 indicates identity Score > 33 indicates homology |
U | R.HPVSPWGFPTK.G | |
| 2470 |
2 |
790.9636 | 1579.9126 | 1579.8933 | 12.2 | 0 | 92 | 9.4e-008 | 1Score > 34 indicates identity |
U | K.GVSAGDQLIAGALAPIK.A |
| 2839 |
587.3417 | 1759.0033 | 1758.9992 | 2.31 | 1 | 6 | 0.61 | 1Score > 33 indicates identity Score > 16 indicates homology |
U | R.NIPVGSTIHGIELKPGK.G | |
| 2840 |
587.6769 | 1760.0089 | 1759.9832 | 14.6 | 1 | 2 | 1.1 | 2Score > 32 indicates identity Score > 14 indicates homology |
U | R.NIPVGSTIHGIELKPGK.G + Deamidated (NQ) | |
| 2841 |
441.0098 | 1760.0101 | 1759.9832 | 15.3 | 1 | 10 | 0.68 | 1Score > 32 indicates identity Score > 21 indicates homology |
U | R.NIPVGSTIHGIELKPGK.G + Deamidated (NQ) |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| RL2_PSE14 |
90 | 29789 | 20.1 | ||
| 50S ribosomal protein L2 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplB PE=3 SV=1 | |||||
| 5 samesets of RL2_PSE14 | |||||
| RL2_PSEFS |
90 | 29803 | |||
| 50S ribosomal protein L2 OS=Pseudomonas fluorescens (strain SBW25) GN=rplB PE=3 SV=1 | |||||
| RL2_PSEPF |
90 | 29773 | |||
| 50S ribosomal protein L2 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplB PE=3 SV=1 | |||||
| RL2_PSESM |
90 | 29833 | |||
| 50S ribosomal protein L2 OS=Pseudomonas syringae pv. tomato GN=rplB PE=3 SV=1 | |||||
| RL2_PSEU2 |
90 | 29789 | |||
| 50S ribosomal protein L2 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplB PE=3 SV=1 | |||||
| RL2_PSEMY |
90 | 29847 | |||
| 50S ribosomal protein L2 OS=Pseudomonas mendocina (strain ymp) GN=rplB PE=3 SV=1 | |||||
| RL2_AZOVD |
42 | 29689 | 20.1 | ||
| 50S ribosomal protein L2 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rplB PE=3 SV=1 | |||||
| 3 samesets of RL2_AZOVD | |||||
| RL2_PSEF5 |
42 | 29745 | |||
| 50S ribosomal protein L2 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplB PE=3 SV=1 | |||||
| RL2_PSEU5 |
42 | 29761 | |||
| 50S ribosomal protein L2 OS=Pseudomonas stutzeri (strain A1501) GN=rplB PE=3 SV=1 | |||||
| RL2_NEOSM |
42 | 30311 | |||
| 50S ribosomal protein L2 OS=Neorickettsia sennetsu (strain Miyayama) GN=rplB PE=3 SV=1 | |||||
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