MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 179)

Page: Previous 1 2 3 4 5 6 7 … 18 Next 

+11

Accession Score Description
1 RL11_PSEP1 307 50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1

+12

Accession Score Description
1 PORF_PSESY 300 Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1

+13

Accession Score Description
1 DBHB_PSEAE 292 DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3

+14

Accession Score Description
Family member distances as a dendrogram 1 RPOC_PSEPG 283 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain GB-1) GN=rpoC PE=3 SV=1
3 RPOC_SYNAS 112 DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1
4 FTSZ_PSEPK 96 Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3
2 RPOB_PSEE4 207 DNA-directed RNA polymerase subunit beta OS=Pseudomonas entomophila (strain L48) GN=rpoB PE=3 SV=1

+15

Accession Score Description
1 ODO2_PSEAE 268 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1

+16

Accession Score Description
1 DHSA_ECOLI 267 Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1

+17

Accession Score Description
1 ARCA_PSEPK 262 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

-18

Accession Score Description
1 ACON2_PSEAE 261 Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1
Score Mass Matches Sequences emPAI
18.1 ACON2_PSEAE 261 94196 15 (8) 10 (5) 0.13
Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1

-15 peptide matches (10 non-duplicate, 5 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
351   626.3888 625.3815 625.3799 2.58 0 18 0.7 +1Score > 32 indicates identity
Score > 29 indicates homology		 U K.IPVVQA.-
649   382.7202 763.4258 763.4228 3.95 0 10 0.38 +1Score > 41 indicates identity
Score > 18 indicates homology		 U K.GFTLAQK.M
660 +2 384.2628 766.5110 766.5065 5.93 0 41 0.0021 +1Score > 27 indicates identity U R.IPLIVGR.G
955   449.2812 896.5478 896.5443 3.91 0 13 0.94 +3Score > 34 indicates identity
Score > 25 indicates homology		 U K.LQPGITLR.D
1033 +1 463.2825 924.5504 924.5433 7.76 0 36 0.053 +1Score > 36 indicates identity U R.LWLAPPTK.M
1785 +1 622.8327 1243.6508 1243.6449 4.82 1 59 3e-005 +1Score > 41 indicates identity
Score > 26 indicates homology		 U R.VPPGVDEAAYVK.A
1933   661.8517 1321.6888 1321.6838 3.83 0 78 1e-006 +1Score > 40 indicates identity
Score > 31 indicates homology		 U R.VQTGSTVVSTSTR.N
2076 +1 694.8542 1387.6938 1387.6772 12.0 1 76 1.8e-006 +1Score > 41 indicates identity
Score > 31 indicates homology		 U R.YLSFDQIAEFR.E
2663   834.4318 1666.8490 1666.8315 10.5 0 73 2.7e-006 +1Score > 40 indicates identity
Score > 29 indicates homology		 U K.GFPVAYVGDVVGTGSSR.K
3115   645.3605 1933.0597 1933.0282 16.3 0 14 0.15 +1Score > 35 indicates identity
Score > 19 indicates homology		 U R.GGVSLRPGDGIIHSWLNR.M

2 subsets and intersections (3 subset proteins in total)

Score Mass Subset of
ACON2_ECOLI 64 94009 18.1
Aconitate hydratase 2 OS=Escherichia coli (strain K12) GN=acnB PE=1 SV=3
1 sameset of ACON2_ECOLI
ACON2_SYNY3 64 94290
Aconitate hydratase 2 OS=Synechocystis sp. (strain PCC 6803) GN=acnB PE=3 SV=1
RIBA_BAUCH 50 22461 18.1
GTP cyclohydrolase-2 OS=Baumannia cicadellinicola subsp. Homalodisca coagulata GN=ribA PE=3 SV=1

+19

Accession Score Description
1 HTPG_PSEPK 255 Chaperone protein htpG OS=Pseudomonas putida (strain KT2440) GN=htpG PE=3 SV=1

+20

Accession Score Description
1 RL2_PSEP1 235 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1
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