MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 171–179 (out of 179)


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+171

Accession Score Description
1 UGPI6_ARATH 23 Uncharacterized GPI-anchored protein At1g61900 OS=Arabidopsis thaliana GN=At1g61900 PE=1 SV=1

+172

Accession Score Description
1 HPPD_PSEUJ 22 4-hydroxyphenylpyruvate dioxygenase OS=Pseudomonas sp. (strain P.J. 874) GN=hpd PE=1 SV=1

-173

Accession Score Description
1 MUTL_ECOBW 22 DNA mismatch repair protein mutL OS=Escherichia coli (strain K12 / BW2952) GN=mutL PE=3 SV=1
Score Mass Matches Sequences emPAI
173.1 MUTL_ECOBW 22 68281 4 (2) 2 (1) 0.03
DNA mismatch repair protein mutL OS=Escherichia coli (strain K12 / BW2952) GN=mutL PE=3 SV=1
+24 samesets of MUTL_ECOBW

-4 peptide matches (3 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1823   636.8405 1271.6664 1271.6431 18.4 0 6 1.3 +6Score > 41 indicates identity
Score > 20 indicates homology
U R.QLLQTPAPMQK.L + 2 Deamidated (NQ); Oxidation (M)
2738   851.4205 1700.8264 1700.8370 -6.18 0 11 0.57 +1Score > 40 indicates identity
Score > 21 indicates homology
U E.LGIDFQSDAQHVTIR.A + 2 Deamidated (NQ)
2740 +1 567.9528 1700.8366 1700.8370 -0.23 0 21 0.024 +1Score > 40 indicates identity
Score > 17 indicates homology
U E.LGIDFQSDAQHVTIR.A + 2 Deamidated (NQ)

+174

Accession Score Description
1 ATM_ASHGO 21 Serine/threonine-protein kinase TEL1 OS=Ashbya gossypii GN=TEL1 PE=3 SV=1

+175

Accession Score Description
1 SIN1_BOVIN 20 Target of rapamycin complex 2 subunit MAPKAP1 OS=Bos taurus GN=MAPKAP1 PE=2 SV=1

+176

Accession Score Description
1 RS19_HAHCH 18 30S ribosomal protein S19 OS=Hahella chejuensis (strain KCTC 2396) GN=rpsS PE=3 SV=1

+177

Accession Score Description
1 YA95_SCHPO 17 Uncharacterized protein C13G6.05c OS=Schizosaccharomyces pombe GN=SPAC13G6.05c PE=1 SV=2

+178

Accession Score Description
1 CATA_BOTFU 17 Catalase A OS=Botryotinia fuckeliana GN=catA PE=2 SV=1

+179

Accession Score Description
1 KCNRG_XENTR 16 Putative potassium channel regulatory protein OS=Xenopus tropicalis GN=kcnrg PE=2 SV=1
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