MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 179)

Page: Previous 1 2 3 4 5 6 7 … 18 Next 

+11

Accession Score Description
1 RL11_PSEP1 307 50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1

+12

Accession Score Description
1 PORF_PSESY 300 Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1

+13

Accession Score Description
1 DBHB_PSEAE 292 DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3

+14

Accession Score Description
Family member distances as a dendrogram 1 RPOC_PSEPG 283 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain GB-1) GN=rpoC PE=3 SV=1
3 RPOC_SYNAS 112 DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1
4 FTSZ_PSEPK 96 Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3
2 RPOB_PSEE4 207 DNA-directed RNA polymerase subunit beta OS=Pseudomonas entomophila (strain L48) GN=rpoB PE=3 SV=1

+15

Accession Score Description
1 ODO2_PSEAE 268 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1

+16

Accession Score Description
1 DHSA_ECOLI 267 Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1

-17

Accession Score Description
1 ARCA_PSEPK 262 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1
Score Mass Matches Sequences emPAI
17.1 ARCA_PSEPK 262 46775 18 (10) 10 (5) 0.27
Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

-18 peptide matches (11 non-duplicate, 7 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1035 +1 463.2919 924.5692 924.5644 5.24 1 29 0.16 +1Score > 34 indicates identity U K.EVIVAGLPK.S
1256   504.7441 1007.4736 1007.4713 2.36 0 17 1.3 +2Score > 42 indicates identity
Score > 31 indicates homology		 U R.DHFDFVTK.M
1426 +1 543.7871 1085.5596 1085.5505 8.39 1 36 0.072 +1Score > 41 indicates identity
Score > 37 indicates homology		 U R.SWLEGLEPR.H
1443 +1 548.2963 1094.5780 1094.5720 5.52 0 53 0.0024 +1Score > 39 indicates identity U R.NTYTNTLLR.K
1789   623.3633 1244.7120 1244.7017 8.35 1 20 0.19 +1Score > 37 indicates identity
Score > 25 indicates homology		 U R.DLVTVFPEVVK.E
1846   640.8598 1279.7050 1279.7023 2.11 1 13 0.59 +1Score > 38 indicates identity
Score > 23 indicates homology		 U R.QETLLTTAIYK.F
2109 +1 701.4066 1400.7986 1400.7776 15.0 0 73 4.8e-006 +1Score > 36 indicates identity
Score > 32 indicates homology		 U R.QAIGQLAQNLFAK.G
2445 +1 785.9304 1569.8462 1569.8363 6.36 1 62 1.5e-005 +1Score > 39 indicates identity
Score > 26 indicates homology		 U K.ITPDTVGVGLTNEVR.S
3269 +1 680.0366 2037.0880 2037.0565 15.5 1 56 2.9e-005 +1Score > 37 indicates identity
Score > 23 indicates homology		 U R.GVDVLEMHNLLTDIVQNK.E
3272   680.3460 2038.0162 2038.0405 -11.9 1 18 0.16 +1Score > 40 indicates identity
Score > 23 indicates homology		 U R.GVDVLEMHNLLTDIVQNK.E + Deamidated (NQ)
3878 +1 816.0691 2445.1855 2445.1448 16.6 1 59 0.00058 +1Score > 39 indicates identity U R.EQWDDGNNVVAIEPGVVIGYDR.N + Deamidated (NQ)

3 subsets and intersections (12 subset proteins in total)

Score Mass Subset of
ARCA_PSEPU 87 46933 17.1
Arginine deiminase OS=Pseudomonas putida GN=arcA PE=1 SV=1
ARCA1_RHIME 71 46140 17.1
Arginine deiminase 1 OS=Rhizobium meliloti GN=arcA1 PE=3 SV=1
2 samesets of ARCA1_RHIME
ARCA_RHIET 71 46140
Arginine deiminase OS=Rhizobium etli GN=arcA PE=3 SV=1
ARCA_RHIE6 71 46196
Arginine deiminase OS=Rhizobium etli (strain CIAT 652) GN=arcA PE=3 SV=1
ARCA2_RHIME 65 46880 17.1
Arginine deiminase 2 OS=Rhizobium meliloti GN=arcA2 PE=3 SV=1
7 samesets of ARCA2_RHIME
ARCA_PSEAE 65 46806
Arginine deiminase OS=Pseudomonas aeruginosa GN=arcA PE=1 SV=2
ARCA_BURMA 65 46422
Arginine deiminase OS=Burkholderia mallei GN=arcA PE=3 SV=1
ARCA_BURPS 65 46422
Arginine deiminase OS=Burkholderia pseudomallei GN=arcA PE=3 SV=1
ARCA_BRAJA 65 46780
Arginine deiminase OS=Bradyrhizobium japonicum GN=arcA PE=3 SV=1
ARCA_MARMS 65 46613
Arginine deiminase OS=Marinomonas sp. (strain MWYL1) GN=arcA PE=3 SV=1
ARCA_PSEMY 65 46334
Arginine deiminase OS=Pseudomonas mendocina (strain ymp) GN=arcA PE=3 SV=1
ARCA_RHILO 65 46031
Arginine deiminase OS=Rhizobium loti GN=arcA PE=3 SV=1

+18

Accession Score Description
1 ACON2_PSEAE 261 Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1

+19

Accession Score Description
1 HTPG_PSEPK 255 Chaperone protein htpG OS=Pseudomonas putida (strain KT2440) GN=htpG PE=3 SV=1

+20

Accession Score Description
1 RL2_PSEP1 235 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1
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