MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 151–160 (out of 179)


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-151

Accession Score Description
1 CCA_AERPE 30 CCA-adding enzyme OS=Aeropyrum pernix GN=cca PE=3 SV=2
Score Mass Matches Sequences emPAI
151.1 CCA_AERPE 30 52088 2 (1) 2 (1) 0.04
CCA-adding enzyme OS=Aeropyrum pernix GN=cca PE=3 SV=2

-2 peptide matches (2 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
935   444.7470 887.4794 887.4712 9.25 0 30 0.021 +1Score > 42 indicates identity
Score > 25 indicates homology
U R.NTAASVTPK.R
1348   522.7973 1043.5800 1043.5723 7.38 1 26 1.9 +2Score > 41 indicates identity U R.NTAASVTPKR.L

+152

Accession Score Description
1 RL24_PSEE4 30 50S ribosomal protein L24 OS=Pseudomonas entomophila (strain L48) GN=rplX PE=3 SV=1

+153

Accession Score Description
1 FKBY_HAEIN 30 Probable FKBP-type peptidyl-prolyl cis-trans isomerase OS=Haemophilus influenzae GN=HI0574 PE=1 SV=1

+154

Accession Score Description
1 GLYA1_PSEF5 30 Serine hydroxymethyltransferase 1 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=glyA1 PE=3 SV=1

+155

Accession Score Description
1 MDH_MYCLE 29 Malate dehydrogenase OS=Mycobacterium leprae GN=mdh PE=3 SV=1

+156

Accession Score Description
1 HYDA_PSEAE 28 D-hydantoinase/dihydropyrimidinase OS=Pseudomonas aeruginosa GN=dht PE=3 SV=1

+157

Accession Score Description
1 HEAT1_DICDI 28 HEAT repeat-containing protein 1 homolog OS=Dictyostelium discoideum GN=heatr1 PE=3 SV=1

+158

Accession Score Description
1 OXAA_RUTMC 28 Inner membrane protein oxaA OS=Ruthia magnifica subsp. Calyptogena magnifica GN=oxaA PE=3 SV=1

+159

Accession Score Description
1 APT_METJA 28 Adenine phosphoribosyltransferase OS=Methanocaldococcus jannaschii GN=apt PE=3 SV=1

+160

Accession Score Description
1 HYDA_PSEPU 27 D-hydantoinase/dihydropyrimidinase OS=Pseudomonas putida GN=dht PE=1 SV=2
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