MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 179)

Page: Previous 1 2 3 4 5 6 7 … 18 Next 

+11

Accession Score Description
1 RL11_PSEP1 307 50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1

+12

Accession Score Description
1 PORF_PSESY 300 Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1

+13

Accession Score Description
1 DBHB_PSEAE 292 DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3

-14

Accession Score Description
Family member distances as a dendrogram 1 RPOC_PSEPG 283 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain GB-1) GN=rpoC PE=3 SV=1
3 RPOC_SYNAS 112 DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1
4 FTSZ_PSEPK 96 Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3
2 RPOB_PSEE4 207 DNA-directed RNA polymerase subunit beta OS=Pseudomonas entomophila (strain L48) GN=rpoB PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
14.1 RPOC_PSEPG 283 155435 24 (13) 18 (10) 0.16
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain GB-1) GN=rpoC PE=3 SV=1
11 samesets of RPOC_PSEPG
RPOC_PSEPK 283 155386 24 (13) 18 (10) 0.16
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1
RPOC_PSEPW 283 155341 24 (13) 18 (10) 0.16
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain W619) GN=rpoC PE=3 SV=1
RPOC_PSEP1 283 155358 24 (13) 18 (10) 0.16
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoC PE=3 SV=1
RPOC_PSEE4 283 155376 24 (13) 18 (10) 0.16
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas entomophila (strain L48) GN=rpoC PE=3 SV=1
RPOC_PSEFS 283 155212 23 (13) 17 (10) 0.16
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas fluorescens (strain SBW25) GN=rpoC PE=3 SV=1
RPOC_PSESM 283 155321 23 (13) 17 (10) 0.16
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas syringae pv. tomato GN=rpoC PE=3 SV=1
RPOC_PSEU2 283 155367 23 (13) 17 (10) 0.16
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpoC PE=3 SV=1
RPOC_PSE14 283 155339 23 (13) 17 (10) 0.16
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpoC PE=3 SV=1
RPOC_PSEPF 283 155342 23 (13) 17 (10) 0.16
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpoC PE=3 SV=1
RPOC_PSEF5 283 155130 23 (13) 17 (10) 0.16
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpoC PE=3 SV=1
RPOC_PSEMY 283 155270 21 (13) 15 (10) 0.16
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas mendocina (strain ymp) GN=rpoC PE=3 SV=1
14.2 RPOB_PSEE4 207 151230 23 (13) 18 (8) 0.14
DNA-directed RNA polymerase subunit beta OS=Pseudomonas entomophila (strain L48) GN=rpoB PE=3 SV=1
4 samesets of RPOB_PSEE4
RPOB_PSEPG 207 151555 26 (13) 20 (8) 0.14
DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1
RPOB_PSEPK 207 151468 26 (13) 20 (8) 0.14
DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain KT2440) GN=rpoB PE=3 SV=1
RPOB_PSEP1 207 151468 26 (13) 20 (8) 0.14
DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoB PE=3 SV=1
RPOB_PSEPW 207 151379 25 (13) 19 (8) 0.14
DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain W619) GN=rpoB PE=3 SV=1
14.3 RPOC_SYNAS 112 154698 7 (5) 6 (4) 0.06
DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1
14.4 FTSZ_PSEPK 96 41918 5 (4) 5 (4) 0.24
Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3

+55 peptide matches (45 non-duplicate, 10 duplicate)

+38 subsets and intersections (890 subset proteins in total)

+15

Accession Score Description
1 ODO2_PSEAE 268 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1

+16

Accession Score Description
1 DHSA_ECOLI 267 Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1

+17

Accession Score Description
1 ARCA_PSEPK 262 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

+18

Accession Score Description
1 ACON2_PSEAE 261 Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1

+19

Accession Score Description
1 HTPG_PSEPK 255 Chaperone protein htpG OS=Pseudomonas putida (strain KT2440) GN=htpG PE=3 SV=1

+20

Accession Score Description
1 RL2_PSEP1 235 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1
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