MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 179)

Page: Previous 1 2 3 4 5 6 7 … 18 Next 

+11

Accession Score Description
1 RL11_PSEP1 307 50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1

+12

Accession Score Description
1 PORF_PSESY 300 Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1

+13

Accession Score Description
1 DBHB_PSEAE 292 DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3

-14

Accession Score Description
Family member distances as a dendrogram 1 RPOC_PSEPG 283 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain GB-1) GN=rpoC PE=3 SV=1
3 RPOC_SYNAS 112 DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1
4 FTSZ_PSEPK 96 Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3
2 RPOB_PSEE4 207 DNA-directed RNA polymerase subunit beta OS=Pseudomonas entomophila (strain L48) GN=rpoB PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
RPOC_PSEPG 283 155435 24 (13) 18 (10) 0.16
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain GB-1) GN=rpoC PE=3 SV=1
11 samesets of RPOC_PSEPG
RPOC_PSEPK 283 155386 24 (13) 18 (10) 0.16
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1
RPOC_PSEPW 283 155341 24 (13) 18 (10) 0.16
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain W619) GN=rpoC PE=3 SV=1
RPOC_PSEP1 283 155358 24 (13) 18 (10) 0.16
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoC PE=3 SV=1
RPOC_PSEE4 283 155376 24 (13) 18 (10) 0.16
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas entomophila (strain L48) GN=rpoC PE=3 SV=1
RPOC_PSEFS 283 155212 23 (13) 17 (10) 0.16
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas fluorescens (strain SBW25) GN=rpoC PE=3 SV=1
RPOC_PSESM 283 155321 23 (13) 17 (10) 0.16
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas syringae pv. tomato GN=rpoC PE=3 SV=1
RPOC_PSEU2 283 155367 23 (13) 17 (10) 0.16
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpoC PE=3 SV=1
RPOC_PSE14 283 155339 23 (13) 17 (10) 0.16
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpoC PE=3 SV=1
RPOC_PSEPF 283 155342 23 (13) 17 (10) 0.16
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpoC PE=3 SV=1
RPOC_PSEF5 283 155130 23 (13) 17 (10) 0.16
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpoC PE=3 SV=1
RPOC_PSEMY 283 155270 21 (13) 15 (10) 0.16
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas mendocina (strain ymp) GN=rpoC PE=3 SV=1
RPOB_PSEE4 207 151230 23 (13) 18 (8) 0.14
DNA-directed RNA polymerase subunit beta OS=Pseudomonas entomophila (strain L48) GN=rpoB PE=3 SV=1
4 samesets of RPOB_PSEE4
RPOB_PSEPG 207 151555 26 (13) 20 (8) 0.14
DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1
RPOB_PSEPK 207 151468 26 (13) 20 (8) 0.14
DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain KT2440) GN=rpoB PE=3 SV=1
RPOB_PSEP1 207 151468 26 (13) 20 (8) 0.14
DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoB PE=3 SV=1
RPOB_PSEPW 207 151379 25 (13) 19 (8) 0.14
DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain W619) GN=rpoB PE=3 SV=1
RPOC_SYNAS 112 154698 7 (5) 6 (4) 0.06
DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1
FTSZ_PSEPK 96 41918 5 (4) 5 (4) 0.24
Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3

-55 peptide matches (45 non-duplicate, 10 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 4 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 4 Peptide
237   516.3044 515.2971 515.2955 3.18 0 13 5 +6Score > 47 indicates identity
Score > 32 indicates homology		 X R.ALAIE.K
239   517.3022 516.2949 516.2907 8.13 0 23 0.7 +1Score > 48 indicates identity
Score > 34 indicates homology		 X E.AINAK.G + Deamidated (NQ)
248   531.2910 530.2837 530.2812 4.69 0 19 1 +4Score > 46 indicates identity
Score > 32 indicates homology		 X K.NIGAR.T + Deamidated (NQ)
282   572.3541 571.3468 571.3442 4.60 0 12 3 +8Score > 42 indicates identity
Score > 29 indicates homology		 X K.AQVVR.I
369   316.6914 631.3682 631.3653 4.63 0 20 3.3 +7Score > 41 indicates identity
Score > 37 indicates homology		 U X E.VTGLSR.K
385   645.3600 644.3527 644.3493 5.31 1 22 3.9 +10Score > 45 indicates identity
Score > 41 indicates homology		 X R.EAINAK.G
547   358.7200 715.4254 715.4228 3.65 0 21 1.4 +5Score > 43 indicates identity
Score > 35 indicates homology		 U X K.TLVDIR.N
590   367.7332 733.4518 733.4486 4.38 0 14 0.52 +1Score > 35 indicates identity
Score > 24 indicates homology		 U X K.VYLAIR.R
604 +1 372.2568 742.4990 742.4953 5.09 0 21 0.44 +1Score > 32 indicates identity
Score > 30 indicates homology		 U X R.VLLGITK.A
625   379.2655 756.5164 756.5109 7.32 0 33 0.011 +1Score > 26 indicates identity U X K.LLTILGK.D
697   392.7379 783.4612 783.4603 1.23 0 28 0.15 +1Score > 37 indicates identity
Score > 33 indicates homology		 X R.QAVPTLR.A
733   400.7371 799.4596 799.4552 5.56 1 12 0.81 +1Score > 42 indicates identity
Score > 23 indicates homology		 U X K.VIVEQGR.R
881 +1 430.7508 859.4870 859.4803 7.80 0 38 0.052 +1Score > 41 indicates identity
Score > 38 indicates homology		 U X K.VIDLWSK.A
898 +1 436.2872 870.5598 870.5538 6.91 0 37 0.014 +1Score > 33 indicates identity
Score > 31 indicates homology		 U X R.LLGVSALAK.Y
921   441.2478 880.4810 880.4766 5.01 1 16 0.58 +2Score > 38 indicates identity
Score > 26 indicates homology		 U X R.HINQLEK.A
1006   458.7784 915.5422 915.5389 3.65 1 40 0.034 +1Score > 39 indicates identity
Score > 38 indicates homology		 U X X R.KGLADTALK.T
1123   317.8633 950.5681 950.5661 2.03 0 26 0.32 +1Score > 34 indicates identity X R.VIVSQLHR.S
1124   318.5078 952.5016 952.4978 3.97 0 16 0.4 +1Score > 39 indicates identity
Score > 24 indicates homology		 X K.LNHLVDDK.M
1138 +1 480.2917 958.5688 958.5600 9.22 0 36 0.036 +1Score > 37 indicates identity
Score > 34 indicates homology		 U X R.GVTFAVPLR.V
1206   494.7901 987.5656 987.5601 5.66 1 36 0.12 +1Score > 39 indicates identity U X R.VLTEAAVTGK.R
1206   494.7901 987.5656 987.5600 5.68 1 19 5.7 +4Score > 39 indicates identity U X R.VLTEASLAGK.V
1215   496.7628 991.5110 991.5087 2.39 0 28 0.67 +1Score > 41 indicates identity
Score > 39 indicates homology		 U X K.AQQYIVDR.R
1290   339.5150 1015.5232 1015.5199 3.19 0 24 0.71 +1Score > 42 indicates identity
Score > 35 indicates homology		 U X R.TFHIGGAASR.T
1307 +1 514.3013 1026.5880 1026.5822 5.70 0 50 0.0029 +1Score > 39 indicates identity
Score > 37 indicates homology		 U X R.VSALGPGGLTR.E
1342   521.8204 1041.6262 1041.6183 7.67 0 39 0.037 +1Score > 38 indicates identity U X R.SVITVGPTLR.L
1362   527.8227 1053.6308 1053.6182 12.0 1 46 0.0041 +1Score > 35 indicates identity U X K.LSLELVPQR.L
1512   565.8450 1129.6754 1129.6707 4.22 0 26 0.012 +1Score > 34 indicates identity
Score > 19 indicates homology		 U X R.TILQLGTGVTK.G
1568 +1 578.3472 1154.6798 1154.6659 12.1 1 56 0.00062 +1Score > 36 indicates identity U X K.QLIDELVAVR.H
1576 +1 578.8228 1155.6310 1155.6499 -16.3 1 48 0.0026 +1Score > 40 indicates identity
Score > 35 indicates homology		 U X K.QLIDELVAVR.H + Deamidated (NQ)
1619   392.8542 1175.5408 1175.5360 4.06 0 24 0.03 +1Score > 41 indicates identity
Score > 22 indicates homology		 U X R.SPGVFFDHDR.G
1654   596.3071 1190.5996 1190.5932 5.45 1 26 0.16 +1Score > 42 indicates identity
Score > 30 indicates homology		 U X R.VFADLQEVDR.V
1762   617.8525 1233.6904 1233.6718 15.1 0 53 9.6e-005 +1Score > 38 indicates identity
Score > 25 indicates homology		 U X K.GTVIDVQVFTR.D
1904 +1 657.8247 1313.6348 1313.6252 7.37 0 41 0.0013 +1Score > 40 indicates identity
Score > 25 indicates homology		 U X X R.FATSDLNDLYR.R
1935 +1 662.9039 1323.7932 1323.7762 12.9 0 62 4.9e-005 +1Score > 32 indicates identity U X R.LLDLSAPDIIVR.N
2128 +1 706.3709 1410.7272 1410.7143 9.17 1 87 1.2e-006 +1Score > 40 indicates identity U X K.YIVNEIQDVYR.L
2262   495.6019 1483.7839 1483.7783 3.75 1 57 7e-005 +1Score > 40 indicates identity
Score > 28 indicates homology		 U X R.LIPAGTGLAYHSER.K
2448   393.9610 1571.8149 1571.8096 3.35 1 13 0.16 +1Score > 40 indicates identity
Score > 17 indicates homology		 U X K.WDPHTHPIVTELK.G
2578   811.9265 1621.8384 1621.8312 4.49 0 85 6.2e-007 +1Score > 40 indicates identity
Score > 36 indicates homology		 U X R.STGSYSLVTQQPLGGK.A
2749   569.6187 1705.8343 1705.8271 4.19 1 74 4.8e-006 +1Score > 41 indicates identity
Score > 33 indicates homology		 U X R.GEVISDGPSDPHDILR.L
2877   593.3237 1776.9493 1776.9159 18.8 1 45 0.0013 +1Score > 38 indicates identity
Score > 28 indicates homology		 U X R.EGLSVLQYFISTHGAR.K
3231   1009.5312 2017.0478 2017.0156 16.0 0 78 6.8e-006 +1Score > 38 indicates identity U X K.LNPQDDLDYLDIPAFLR.R
3257   1017.0081 2032.0016 2031.9749 13.2 1 65 4.2e-006 +1Score > 40 indicates identity
Score > 24 indicates homology		 U X K.ASLSTQSFISAASFQETTR.V + Deamidated (NQ)
3571   736.3868 2206.1386 2206.0946 19.9 1 21 0.032 +1Score > 38 indicates identity
Score > 19 indicates homology		 U X K.SVFPIISYSGNAALEYVGYR.L + Deamidated (NQ)
3791   791.4053 2371.1941 2371.1768 7.29 1 36 0.00098 +1Score > 38 indicates identity
Score > 18 indicates homology		 U X K.VVDNTLQTAQQAYEASNPAPVR.Q

+38 subsets and intersections (890 subset proteins in total)

+15

Accession Score Description
1 ODO2_PSEAE 268 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1

+16

Accession Score Description
1 DHSA_ECOLI 267 Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1

+17

Accession Score Description
1 ARCA_PSEPK 262 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

+18

Accession Score Description
1 ACON2_PSEAE 261 Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1

+19

Accession Score Description
1 HTPG_PSEPK 255 Chaperone protein htpG OS=Pseudomonas putida (strain KT2440) GN=htpG PE=3 SV=1

+20

Accession Score Description
1 RL2_PSEP1 235 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1
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