MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 121–130 (out of 179)


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+121

Accession Score Description
1 TCPI_VIBCH 40 Toxin coregulated pilus biosynthesis protein I OS=Vibrio cholerae GN=tcpI PE=3 SV=1

+122

Accession Score Description
1 MREB_BACSU 40 Rod shape-determining protein mreB OS=Bacillus subtilis GN=mreB PE=3 SV=3

+123

Accession Score Description
1 RS13_PSEE4 40 30S ribosomal protein S13 OS=Pseudomonas entomophila (strain L48) GN=rpsM PE=3 SV=1

+124

Accession Score Description
1 APT_CAMJD 39 Adenine phosphoribosyltransferase OS=Campylobacter jejuni subsp. doylei (strain ATCC BAA-1458 / RM4099 / 269.97) GN=apt PE=3 SV=1

+125

Accession Score Description
1 RS17_PSE14 39 30S ribosomal protein S17 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsQ PE=3 SV=1

+126

Accession Score Description
1 SUCC_COXBN 39 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Coxiella burnetii (strain Dugway 5J108-111) GN=sucC PE=3 SV=1

+127

Accession Score Description
1 RECA_PSEFL 38 Protein recA OS=Pseudomonas fluorescens GN=recA PE=3 SV=1

+128

Accession Score Description
1 RS1_PSEAE 38 30S ribosomal protein S1 OS=Pseudomonas aeruginosa GN=rpsA PE=3 SV=1

-129

Accession Score Description
1 PROD_CAEEL 37 Proline dehydrogenase, mitochondrial OS=Caenorhabditis elegans GN=B0513.5 PE=3 SV=2
Score Mass Matches Sequences emPAI
129.1 PROD_CAEEL 37 70292 2 (1) 2 (1) 0.03
Proline dehydrogenase, mitochondrial OS=Caenorhabditis elegans GN=B0513.5 PE=3 SV=2
8 samesets of PROD_CAEEL
AP1B1_BOVIN 37 106551 3 (1) 3 (1) 0.02
AP-1 complex subunit beta-1 OS=Bos taurus GN=AP2B1 PE=2 SV=1
AP2B1_BOVIN 37 105412 3 (1) 3 (1) 0.02
AP-2 complex subunit beta OS=Bos taurus GN=AP2B1 PE=1 SV=2
AP2B1_HUMAN 37 105398 3 (1) 3 (1) 0.02
AP-2 complex subunit beta OS=Homo sapiens GN=AP2B1 PE=1 SV=1
AP2B1_MOUSE 37 105428 3 (1) 3 (1) 0.02
AP-2 complex subunit beta OS=Mus musculus GN=Ap2b1 PE=1 SV=1
AP2B1_RAT 37 105398 3 (1) 3 (1) 0.02
AP-2 complex subunit beta OS=Rattus norvegicus GN=Ap2b1 PE=1 SV=1
RSMG_DESPS 37 24588 1 (1) 1 (1) 0.09
Ribosomal RNA small subunit methyltransferase G OS=Desulfotalea psychrophila GN=rsmG PE=3 SV=1
YNW6_SCHPO 37 54918 1 (1) 1 (1) 0.04
Uncharacterized RNA-binding protein P16F5.06 OS=Schizosaccharomyces pombe GN=SPBP16F5.06 PE=1 SV=1
S26A8_BOVIN 37 109758 1 (1) 1 (1) 0.02
Testis anion transporter 1 OS=Bos taurus GN=SLC26A8 PE=2 SV=1

-2 peptide matches (2 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
421   335.7357 669.4568 669.4537 4.65 0 37 0.02 +1Score > 33 indicates identity U R.ALVVLR.L
1763   618.3079 1234.6012 1234.5976 2.97 1 3 1.1 +2Score > 41 indicates identity
Score > 16 indicates homology
U K.ALTGGMSLQEGR.L + Oxidation (M)

+130

Accession Score Description
1 GLPD_PSETO 37 Glycerol-3-phosphate dehydrogenase OS=Pseudomonas tolaasii GN=glpD PE=3 SV=1
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