MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 121–130 (out of 179)

Page: Previous 1 …8 9 10 11 12 13 14 15 16 17 18 Next 

+121

Accession Score Description
1 TCPI_VIBCH 40 Toxin coregulated pilus biosynthesis protein I OS=Vibrio cholerae GN=tcpI PE=3 SV=1

+122

Accession Score Description
1 MREB_BACSU 40 Rod shape-determining protein mreB OS=Bacillus subtilis GN=mreB PE=3 SV=3

+123

Accession Score Description
1 RS13_PSEE4 40 30S ribosomal protein S13 OS=Pseudomonas entomophila (strain L48) GN=rpsM PE=3 SV=1

+124

Accession Score Description
1 APT_CAMJD 39 Adenine phosphoribosyltransferase OS=Campylobacter jejuni subsp. doylei (strain ATCC BAA-1458 / RM4099 / 269.97) GN=apt PE=3 SV=1

+125

Accession Score Description
1 RS17_PSE14 39 30S ribosomal protein S17 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsQ PE=3 SV=1

+126

Accession Score Description
1 SUCC_COXBN 39 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Coxiella burnetii (strain Dugway 5J108-111) GN=sucC PE=3 SV=1

+127

Accession Score Description
1 RECA_PSEFL 38 Protein recA OS=Pseudomonas fluorescens GN=recA PE=3 SV=1

-128

Accession Score Description
1 RS1_PSEAE 38 30S ribosomal protein S1 OS=Pseudomonas aeruginosa GN=rpsA PE=3 SV=1
Score Mass Matches Sequences emPAI
128.1 RS1_PSEAE 38 61946 12 (3) 7 (1) 0.08
30S ribosomal protein S1 OS=Pseudomonas aeruginosa GN=rpsA PE=3 SV=1
7 samesets of RS1_PSEAE
RS1_PROSP 38 41702 10 (3) 5 (1) 0.11
30S ribosomal protein S1 (Fragment) OS=Providencia sp. GN=rpsA PE=3 SV=1
RS1_BUCAI 38 62849 8 (3) 4 (1) 0.07
30S ribosomal protein S1 OS=Buchnera aphidicola subsp. Acyrthosiphon pisum GN=rpsA PE=3 SV=1
RS1_BUCAP 38 62605 8 (3) 4 (1) 0.07
30S ribosomal protein S1 OS=Buchnera aphidicola subsp. Schizaphis graminum GN=rpsA PE=3 SV=1
RS1_ECO57 38 61235 8 (3) 4 (1) 0.08
30S ribosomal protein S1 OS=Escherichia coli O157:H7 GN=rpsA PE=3 SV=1
RS1_ECOL6 38 61235 8 (3) 4 (1) 0.08
30S ribosomal protein S1 OS=Escherichia coli O6 GN=rpsA PE=3 SV=1
RS1_ECOLI 38 61235 8 (3) 4 (1) 0.08
30S ribosomal protein S1 OS=Escherichia coli (strain K12) GN=rpsA PE=1 SV=1
RS1_SHIFL 38 61235 8 (3) 4 (1) 0.08
30S ribosomal protein S1 OS=Shigella flexneri GN=rpsA PE=3 SV=1

-12 peptide matches (9 non-duplicate, 3 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
338   308.7062 615.3978 615.3956 3.73 0 27 0.15 +1Score > 42 indicates identity
Score > 31 indicates homology		 R.VSLGLK.Q
362 +2 315.7141 629.4136 629.4112 3.90 0 29 0.32 +1Score > 38 indicates identity
Score > 36 indicates homology		 R.ISLGIK.Q
410   331.6784 661.3422 661.3395 4.19 1 29 0.24 +1Score > 41 indicates identity
Score > 35 indicates homology		 K.ASEISR.D
707   394.2264 786.4382 786.4348 4.39 0 21 0.9 +1Score > 44 indicates identity
Score > 33 indicates homology		 R.NNVVVSR.R
962   300.8199 899.4379 899.4349 3.35 1 16 1.3 +2Score > 39 indicates identity
Score > 29 indicates homology		 U R.DTTHLEGK.E
963   450.7268 899.4390 899.4349 4.66 1 17 0.19 +1Score > 39 indicates identity
Score > 22 indicates homology		 U R.DTTHLEGK.E
1527   567.7982 1133.5818 1133.5829 -0.97 0 26 0.11 +1Score > 42 indicates identity
Score > 29 indicates homology		 U K.GGFTVDVNGIR.A
2352   763.4542 1524.8938 1524.8777 10.6 0 24 0.039 +1Score > 33 indicates identity
Score > 23 indicates homology		 U R.AFLPGSLVDVRPVR.D
2354 +1 509.3056 1524.8950 1524.8777 11.4 0 36 0.0017 +1Score > 33 indicates identity
Score > 20 indicates homology		 U R.AFLPGSLVDVRPVR.D

+129

Accession Score Description
1 PROD_CAEEL 37 Proline dehydrogenase, mitochondrial OS=Caenorhabditis elegans GN=B0513.5 PE=3 SV=2

+130

Accession Score Description
1 GLPD_PSETO 37 Glycerol-3-phosphate dehydrogenase OS=Pseudomonas tolaasii GN=glpD PE=3 SV=1
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