MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 121–130 (out of 179)


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+121

Accession Score Description
1 TCPI_VIBCH 40 Toxin coregulated pilus biosynthesis protein I OS=Vibrio cholerae GN=tcpI PE=3 SV=1

+122

Accession Score Description
1 MREB_BACSU 40 Rod shape-determining protein mreB OS=Bacillus subtilis GN=mreB PE=3 SV=3

-123

Accession Score Description
1 RS13_PSEE4 40 30S ribosomal protein S13 OS=Pseudomonas entomophila (strain L48) GN=rpsM PE=3 SV=1
Score Mass Matches Sequences emPAI
123.1 RS13_PSEE4 40 13362 4 (2) 3 (2) 0.39
30S ribosomal protein S13 OS=Pseudomonas entomophila (strain L48) GN=rpsM PE=3 SV=1
10 samesets of RS13_PSEE4
RS13_PSEP1 40 13362 4 (2) 3 (2) 0.39
30S ribosomal protein S13 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsM PE=3 SV=1
RS13_PSEPG 40 13362 4 (2) 3 (2) 0.39
30S ribosomal protein S13 OS=Pseudomonas putida (strain GB-1) GN=rpsM PE=3 SV=1
RS13_PSEPK 40 13362 4 (2) 3 (2) 0.39
30S ribosomal protein S13 OS=Pseudomonas putida (strain KT2440) GN=rpsM PE=3 SV=1
RS13_PSEPW 40 13348 4 (2) 3 (2) 0.39
30S ribosomal protein S13 OS=Pseudomonas putida (strain W619) GN=rpsM PE=3 SV=1
RS13_PSE14 40 13475 3 (2) 2 (2) 0.39
30S ribosomal protein S13 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsM PE=3 SV=1
RS13_PSEF5 40 13445 3 (2) 2 (2) 0.39
30S ribosomal protein S13 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsM PE=3 SV=1
RS13_PSEFS 40 13446 3 (2) 2 (2) 0.39
30S ribosomal protein S13 OS=Pseudomonas fluorescens (strain SBW25) GN=rpsM PE=3 SV=1
RS13_PSEPF 40 13475 3 (2) 2 (2) 0.39
30S ribosomal protein S13 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsM PE=3 SV=1
RS13_PSESM 40 13475 3 (2) 2 (2) 0.39
30S ribosomal protein S13 OS=Pseudomonas syringae pv. tomato GN=rpsM PE=3 SV=1
RS13_PSEU2 40 13475 3 (2) 2 (2) 0.39
30S ribosomal protein S13 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpsM PE=3 SV=1

-4 peptide matches (3 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
419   335.2252 668.4358 668.4333 3.76 1 14 0.16 +1Score > 31 indicates identity
Score > 19 indicates homology
R.KGLPVR.G
1071   469.7340 937.4534 937.4505 3.13 1 36 0.044 +1Score > 41 indicates identity
Score > 35 indicates homology
U K.FTTEGDLR.R
1339 +1 520.7933 1039.5720 1039.5662 5.63 0 37 0.02 +1Score > 39 indicates identity
Score > 32 indicates homology
U R.IAGVNIPDNK.H

2 subsets and intersections (25 subset proteins in total)

Score Mass Subset of
RS13_ACIAD 37 13198 123.1
30S ribosomal protein S13 OS=Acinetobacter sp. (strain ADP1) GN=rpsM PE=3 SV=1
+23 samesets of RS13_ACIAD
RS13_MYCMO 36 14001 123.1
30S ribosomal protein S13 OS=Mycoplasma mobile GN=rpsM PE=3 SV=1

+124

Accession Score Description
1 APT_CAMJD 39 Adenine phosphoribosyltransferase OS=Campylobacter jejuni subsp. doylei (strain ATCC BAA-1458 / RM4099 / 269.97) GN=apt PE=3 SV=1

+125

Accession Score Description
1 RS17_PSE14 39 30S ribosomal protein S17 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsQ PE=3 SV=1

+126

Accession Score Description
1 SUCC_COXBN 39 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Coxiella burnetii (strain Dugway 5J108-111) GN=sucC PE=3 SV=1

+127

Accession Score Description
1 RECA_PSEFL 38 Protein recA OS=Pseudomonas fluorescens GN=recA PE=3 SV=1

+128

Accession Score Description
1 RS1_PSEAE 38 30S ribosomal protein S1 OS=Pseudomonas aeruginosa GN=rpsA PE=3 SV=1

+129

Accession Score Description
1 PROD_CAEEL 37 Proline dehydrogenase, mitochondrial OS=Caenorhabditis elegans GN=B0513.5 PE=3 SV=2

+130

Accession Score Description
1 GLPD_PSETO 37 Glycerol-3-phosphate dehydrogenase OS=Pseudomonas tolaasii GN=glpD PE=3 SV=1
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