MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita2 sp |
| MS data file | : | PRT1270_T-BRSC_2_20250714115216.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:39:15 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,778 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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11| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL11_PSEP1 | 307 | 50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1 |
12| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PORF_PSESY | 300 | Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 12.1 | PORF_PSESY |
300 | 36773 | 17 (7) | 4 (2) | 0.28 | |
| Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1 | |||||||
17 peptide matches (8 non-duplicate, 9 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 422 |
2 |
336.1564 | 670.2982 | 670.2962 | 2.98 | 0 | 20 | 0.87 | 1Score > 37 indicates identity Score > 32 indicates homology |
K.FDFDK.S | |
| 424 |
1 |
671.3060 | 670.2987 | 670.2962 | 3.69 | 0 | 27 | 0.32 | 1Score > 37 indicates identity Score > 35 indicates homology |
K.FDFDK.S | |
| 508 |
1 |
702.4052 | 701.3979 | 701.3959 | 2.81 | 1 | 42 | 0.07 | 1Score > 43 indicates identity |
R.VELDVK.F | |
| 510 |
2 |
351.7064 | 701.3982 | 701.3959 | 3.28 | 1 | 35 | 0.37 | 5Score > 43 indicates identity |
R.VELDVK.F | |
| 1893 |
1 |
436.2394 | 1305.6964 | 1305.6929 | 2.69 | 0 | 48 | 0.00062 | 1Score > 40 indicates identity Score > 28 indicates homology |
U | K.SVVKPNSYGDIK.N |
| 1895 |
1 |
653.8560 | 1305.6974 | 1305.6929 | 3.52 | 0 | 65 | 9.9e-005 | 1Score > 40 indicates identity Score > 37 indicates homology |
U | K.SVVKPNSYGDIK.N |
| 3707 |
1 |
1152.0657 | 2302.1168 | 2302.0753 | 18.0 | 1 | 126 | 4.5e-011 | 1Score > 39 indicates identity Score > 35 indicates homology |
U | K.NNGNLFGGSIGYFLTDDVELR.L + 2 Deamidated (NQ) |
| 3708 |
768.3802 | 2302.1188 | 2302.0753 | 18.9 | 1 | 25 | 0.026 | 1Score > 39 indicates identity Score > 22 indicates homology |
U | K.NNGNLFGGSIGYFLTDDVELR.L + 2 Deamidated (NQ) |
13| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DBHB_PSEAE | 292 | DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3 |
14| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOC_PSEPG | 283 | DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain GB-1) GN=rpoC PE=3 SV=1 | |
| 3 | RPOC_SYNAS | 112 | DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1 | |
| 4 | FTSZ_PSEPK | 96 | Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3 | |
| 2 | RPOB_PSEE4 | 207 | DNA-directed RNA polymerase subunit beta OS=Pseudomonas entomophila (strain L48) GN=rpoB PE=3 SV=1 | |
15| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODO2_PSEAE | 268 | Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1 |
16| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DHSA_ECOLI | 267 | Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1 |
17| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ARCA_PSEPK | 262 | Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1 |
18| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ACON2_PSEAE | 261 | Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1 |
19| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HTPG_PSEPK | 255 | Chaperone protein htpG OS=Pseudomonas putida (strain KT2440) GN=htpG PE=3 SV=1 |
20| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL2_PSEP1 | 235 | 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1 |
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