MASCOT Search Results

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User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 179)

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-11

Accession Score Description
1 RL11_PSEP1 307 50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1
Score Mass Matches Sequences emPAI
11.1 RL11_PSEP1 307 14914 16 (7) 8 (4) 0.81
50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1
3 samesets of RL11_PSEP1
RL11_PSEPG 307 14914 16 (7) 8 (4) 0.81
50S ribosomal protein L11 OS=Pseudomonas putida (strain GB-1) GN=rplK PE=3 SV=1
RL11_PSEPK 307 14914 16 (7) 8 (4) 0.81
50S ribosomal protein L11 OS=Pseudomonas putida (strain KT2440) GN=rplK PE=3 SV=1
RL11_PSEPW 307 14914 16 (7) 8 (4) 0.81
50S ribosomal protein L11 OS=Pseudomonas putida (strain W619) GN=rplK PE=3 SV=1

-16 peptide matches (9 non-duplicate, 7 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
369 +1 316.6914 631.3682 631.3653 4.60 0 29 0.37 +1Score > 41 indicates identity
Score > 37 indicates homology		 K.VGTVTR.A
456 +1 338.1944 674.3742 674.3711 4.63 0 21 0.44 +1Score > 40 indicates identity
Score > 30 indicates homology		 R.TIAGSAR.S
871   430.2147 858.4148 858.4123 2.92 1 28 0.074 +1Score > 40 indicates identity
Score > 29 indicates homology		 R.SFTFETK.S
929 +1 444.2471 886.4796 886.4760 4.15 1 48 0.018 +1Score > 43 indicates identity U R.AQLEDIAK.A
1000 +2 458.2816 914.5486 914.5437 5.44 0 23 0.086 +1Score > 39 indicates identity
Score > 25 indicates homology		 K.STPASVLLK.K
2166 +1 715.3716 1428.7286 1428.7209 5.45 0 93 9e-008 +1Score > 40 indicates identity
Score > 35 indicates homology		 U K.QADLTAADLDAAVR.T
2167   715.3946 1428.7746 1428.7685 4.32 0 49 0.0035 +1Score > 40 indicates identity
Score > 37 indicates homology		 U K.AAGLTSGSARPNTVK.V
2168   477.2655 1428.7747 1428.7685 4.34 0 18 0.15 +1Score > 40 indicates identity
Score > 23 indicates homology		 U K.AAGLTSGSARPNTVK.V
3453 +1 1073.5747 2145.1348 2145.0954 18.4 1 111 7e-010 +1Score > 37 indicates identity
Score > 32 indicates homology		 U R.TQGQEAGLPTPVIITVYSDR.S + Deamidated (NQ)

6 subsets and intersections (16 subset proteins in total)

Score Mass Subset of
RL11_PSEE4 164 14940 11.1
50S ribosomal protein L11 OS=Pseudomonas entomophila (strain L48) GN=rplK PE=3 SV=1
RL11_PSEMY 153 14959 11.1
50S ribosomal protein L11 OS=Pseudomonas mendocina (strain ymp) GN=rplK PE=3 SV=1
RL11_CHRVO 141 15002 11.1
50S ribosomal protein L11 OS=Chromobacterium violaceum GN=rplK PE=3 SV=1
4 samesets of RL11_CHRVO
RL11_PSEA7 141 14955
50S ribosomal protein L11 OS=Pseudomonas aeruginosa (strain PA7) GN=rplK PE=3 SV=1
RL11_PSEA8 141 14955
50S ribosomal protein L11 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplK PE=3 SV=1
RL11_PSEAB 141 14955
50S ribosomal protein L11 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplK PE=3 SV=1
RL11_PSEAE 141 14955
50S ribosomal protein L11 OS=Pseudomonas aeruginosa GN=rplK PE=3 SV=1
RL11_PSESM 61 14930 11.1
50S ribosomal protein L11 OS=Pseudomonas syringae pv. tomato GN=rplK PE=3 SV=1
1 sameset of RL11_PSESM
RL11_PSEU2 61 14930
50S ribosomal protein L11 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplK PE=3 SV=1
RL11_ACIF2 50 15022 11.1
50S ribosomal protein L11 OS=Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) GN=rplK PE=3 SV=1
3 samesets of RL11_ACIF2
RL11_PSE14 50 14911
50S ribosomal protein L11 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplK PE=3 SV=1
RL11_ACIF5 50 15022
50S ribosomal protein L11 OS=Acidithiobacillus ferrooxidans (strain ATCC 53993) GN=rplK PE=3 SV=1
RL11_PRB01 50 14968
50S ribosomal protein L11 OS=Gamma-proteobacterium EBAC31A08 GN=rplK PE=3 SV=1
RL11_PSEF5 49 14960 11.1
50S ribosomal protein L11 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplK PE=3 SV=1
2 samesets of RL11_PSEF5
RL11_PSEFS 49 14960
50S ribosomal protein L11 OS=Pseudomonas fluorescens (strain SBW25) GN=rplK PE=3 SV=1
RL11_PSEPF 49 14934
50S ribosomal protein L11 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplK PE=3 SV=1

+12

Accession Score Description
1 PORF_PSESY 300 Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1

+13

Accession Score Description
1 DBHB_PSEAE 292 DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3

+14

Accession Score Description
Family member distances as a dendrogram 1 RPOC_PSEPG 283 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain GB-1) GN=rpoC PE=3 SV=1
3 RPOC_SYNAS 112 DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1
4 FTSZ_PSEPK 96 Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3
2 RPOB_PSEE4 207 DNA-directed RNA polymerase subunit beta OS=Pseudomonas entomophila (strain L48) GN=rpoB PE=3 SV=1

+15

Accession Score Description
1 ODO2_PSEAE 268 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1

+16

Accession Score Description
1 DHSA_ECOLI 267 Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1

+17

Accession Score Description
1 ARCA_PSEPK 262 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

+18

Accession Score Description
1 ACON2_PSEAE 261 Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1

+19

Accession Score Description
1 HTPG_PSEPK 255 Chaperone protein htpG OS=Pseudomonas putida (strain KT2440) GN=htpG PE=3 SV=1

+20

Accession Score Description
1 RL2_PSEP1 235 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1
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