MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 179)

Page: 1 2 3 4 5 6 … 18 Next 

-1

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1121 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
6 EFTU_CYAP7 40 Elongation factor Tu OS=Cyanothece sp. (strain PCC 7424) GN=tuf PE=3 SV=1
5 EFTU_ANATD 50 Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1
3 EFTU_PSEFS 701 Elongation factor Tu OS=Pseudomonas fluorescens (strain SBW25) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1002 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1
4 EFTU_CARRP 145 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
EFTU2_PSEPK 1121 43793 87 (56) 18 (16) 3.17
Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
1 sameset of EFTU2_PSEPK
EFTU_PSEE4 1121 43793 87 (56) 18 (16) 3.17
Elongation factor Tu OS=Pseudomonas entomophila (strain L48) GN=tuf1 PE=3 SV=1
EFTU1_PSEPK 1002 43810 83 (52) 18 (16) 2.97
Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1
3 samesets of EFTU1_PSEPK
EFTU_PSEPG 1002 43810 83 (52) 18 (16) 2.97
Elongation factor Tu OS=Pseudomonas putida (strain GB-1) GN=tuf1 PE=3 SV=1
EFTU_PSEPW 1002 43810 83 (52) 18 (16) 2.97
Elongation factor Tu OS=Pseudomonas putida (strain W619) GN=tuf1 PE=3 SV=1
EFTU_PSEP1 1002 43810 83 (52) 18 (16) 2.97
Elongation factor Tu OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=tuf PE=3 SV=1
EFTU_PSEFS 701 43878 67 (40) 14 (12) 2.07
Elongation factor Tu OS=Pseudomonas fluorescens (strain SBW25) GN=tuf PE=3 SV=1
EFTU_CARRP 145 44439 14 (7) 2 (2) 0.16
Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
EFTU_ANATD 50 44208 10 (2) 4 (2) 0.11
Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1
1 sameset of EFTU_ANATD
EFTU_CALS8 50 44218 10 (2) 4 (2) 0.11
Elongation factor Tu OS=Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903) GN=tuf PE=3 SV=1
EFTU_CYAP7 40 44885 8 (3) 3 (3) 0.16
Elongation factor Tu OS=Cyanothece sp. (strain PCC 7424) GN=tuf PE=3 SV=1

-113 peptide matches (53 non-duplicate, 60 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 4 5 6 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 4 5 6 Peptide
478 +2 345.1998 688.3850 688.3868 -2.56 0 31 0.11 +1Score > 43 indicates identity
Score > 34 indicates homology		 X X X X R.GTVVTGR  
480   689.3965 688.3892 688.3868 3.51 0 28 0.64 +1Score > 43 indicates identity
Score > 39 indicates homology		 X X X X R.GTVVTGR  
503 +2 351.6938 701.3730 701.3708 3.22 1 28 0.34 +1Score > 45 indicates identity
Score > 35 indicates homology		 U X X X K.LLDEGR.A
504 +1 702.3806 701.3733 701.3708 3.61 1 45 0.05 +1Score > 45 indicates identity
Score > 44 indicates homology		 U X X X K.LLDEGR.A
677   776.4066 775.3993 775.4017 -3.09 0 21 0.24 +1Score > 42 indicates identity
Score > 28 indicates homology		 U X X X X X R.HTPFFK  
678 +3 388.7094 775.4042 775.4017 3.26 0 29 0.0095 +1Score > 42 indicates identity
Score > 21 indicates homology		 U X X X X X R.HTPFFK  
735 +2 401.2463 800.4780 800.4756 3.04 0 42 0.0049 +1Score > 42 indicates identity
Score > 32 indicates homology		 U X X X R.TVGAGVVAK.I
737 +2 801.4860 800.4787 800.4756 3.88 0 54 0.0031 +1Score > 42 indicates identity U X X X R.TVGAGVVAK.I
890   867.5077 866.5004 866.4974 3.52 1 54 0.00097 +1Score > 37 indicates identity U X X X R.EHILLSR.Q
891 +2 434.2578 866.5010 866.4974 4.24 1 39 0.033 +1Score > 36 indicates identity U X X X R.EHILLSR.Q
1102 +2 947.5558 946.5485 946.5447 4.00 0 60 8.3e-005 +1Score > 38 indicates identity
Score > 31 indicates homology		 U X X X K.TTLTAALTR.V
1104 +3 474.2818 946.5490 946.5447 4.55 0 75 2.4e-006 +1Score > 38 indicates identity
Score > 31 indicates homology		 U X X X K.TTLTAALTR.V
1250 +1 503.2568 1004.4990 1004.4961 2.97 1 32 0.26 +1Score > 42 indicates identity
Score > 39 indicates homology		 U X X X K.TIAMEDGLR.F
1433   544.7827 1087.5508 1087.5444 5.91 1 57 0.0001 +1Score > 42 indicates identity
Score > 30 indicates homology		 U X X X R.AGENCGVLLR.G
1571   1156.6322 1155.6249 1155.6176 6.36 1 55 0.0015 +1Score > 40 indicates identity U X X K.FTAEVYVLSK.E
1572 +5 578.8209 1155.6272 1155.6176 8.38 1 48 0.00086 +1Score > 40 indicates identity
Score > 30 indicates homology		 U X X K.FTAEVYVLSK.E
1756 +1 617.3130 1232.6114 1232.6091 1.93 0 15 0.61 +1Score > 42 indicates identity
Score > 25 indicates homology		 U X X X K.GYRPQFYFR.T
1757 +2 411.8787 1232.6143 1232.6091 4.22 0 30 0.019 +1Score > 42 indicates identity
Score > 25 indicates homology		 U X X X K.GYRPQFYFR.T
1768   413.5790 1237.7152 1237.7030 9.81 1 31 0.037 +2Score > 36 indicates identity
Score > 30 indicates homology		 U X X X VQDPLEIVGLR.D
1769 +3 619.8663 1237.7180 1237.7030 12.1 1 69 2.3e-005 +1Score > 35 indicates identity U X X X VQDPLEIVGLR.D
2243 +1 492.6312 1474.8718 1474.8548 11.5 0 46 0.0021 +1Score > 31 indicates identity U X X R.QVGVPYIVVFLNK.A
2244 +3 738.4448 1474.8750 1474.8548 13.7 0 66 1.9e-005 +1Score > 31 indicates identity U X X R.QVGVPYIVVFLNK.A
2371   766.9556 1531.8966 1531.8763 13.3 0 40 0.00038 +1Score > 32 indicates identity
Score > 19 indicates homology		 U X R.QVNVPYIVVFLNK.V
2455   787.9745 1573.9344 1573.9304 2.56 0 60 4.8e-005 +1Score > 30 indicates identity U X X X R.GQVLVKPGSVKPHTK.F
2455   787.9745 1573.9344 1573.9304 2.57 0 24 0.18 +2Score > 30 indicates identity U X R.GQVLAKPGTIKPHTK.F
2457   315.7943 1573.9351 1573.9304 2.99 0 9 0.21 +1Score > 30 indicates identity
Score > 15 indicates homology		 U X X X R.GQVLVKPGSVKPHTK.F
2459 +1 394.4913 1573.9361 1573.9304 3.61 0 18 0.048 +1Score > 30 indicates identity
Score > 17 indicates homology		 U X X X R.GQVLVKPGSVKPHTK.F
2460 +1 525.6527 1573.9363 1573.9304 3.72 0 39 0.0043 +1Score > 30 indicates identity
Score > 28 indicates homology		 U X X X R.GQVLVKPGSVKPHTK.F
2460 +1 525.6527 1573.9363 1573.9304 3.73 0 13 1.7 +2Score > 30 indicates identity
Score > 28 indicates homology		 U X R.GQVLAKPGTIKPHTK.F
2463   315.9949 1574.9381 1574.9144 15.0 0 7 0.24 +1Score > 29 indicates identity
Score > 13 indicates homology		 U X X X R.GQVLVKPGSVKPHTK.F + Deamidated (NQ)
2559 +4 807.9503 1613.8860 1613.8665 12.1 1 91 9e-008 +1Score > 38 indicates identity
Score > 33 indicates homology		 U X K.LVETLDAYIPEPVR.A
2561   538.9731 1613.8975 1613.8665 19.2 1 18 0.26 +1Score > 37 indicates identity
Score > 24 indicates homology		 U X K.LVETLDAYIPEPVR.A
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_2_20250714115216.raw

Score > 39 indicates identity

Score > 38 indicates homology
2590 +2 815.9476 1629.8806 1629.8614 11.8 1 93 1.9e-007 -1Score > 39 indicates identity
Score > 38 indicates homology		 U X K.LVETLDSYIPEPVR.A
11.8 0 20 3.8 2 IIDITNIHFTDTVK   + Deamidated (NQ)
-13.4 1 17 7.7 3 NKLSLFLGCIVPNR  
7.43 1 11 28 4 LQSLNLNSINIRSR   + 3 Deamidated (NQ)
7.43 1 10 35 5 LQSLNLNSINTRVR   + 3 Deamidated (NQ)
17.9 1 10 39 6 LVRQVNYASAFSFK   + Deamidated (NQ)
11.8 0 10 39 7 LNSAGINILPQLTFE   + Deamidated (NQ)
6.88 1 9 53 8 IMNMNQRLLLPVR   + Deamidated (NQ); 2 Oxidation (M)
6.88 1 9 53 8 IMNMNQRLLLPVR   + Deamidated (NQ); 2 Oxidation (M)
6.88 1 9 53 8 IMNMNQRLLLPVR   + Deamidated (NQ); 2 Oxidation (M)
2591   544.3023 1629.8851 1629.8614 14.5 1 13 0.5 +1Score > 38 indicates identity
Score > 22 indicates homology		 U X K.LVETLDSYIPEPVR.A
2852 +1 354.1932 1765.9296 1765.9224 4.09 0 30 0.4 +1Score > 38 indicates identity U X X X R.SLPHVNVGTIGHVDHGK.T
2855   354.3935 1766.9311 1766.9064 14.0 0 22 0.27 +1Score > 38 indicates identity
Score > 29 indicates homology		 U X X X R.SLPHVNVGTIGHVDHGK.T + Deamidated (NQ)
2856   884.4730 1766.9314 1766.9064 14.2 0 72 4.3e-007 +1Score > 39 indicates identity
Score > 21 indicates homology		 U X X X R.SLPHVNVGTIGHVDHGK.T + Deamidated (NQ)
2857 +2 442.7405 1766.9329 1766.9064 15.0 0 36 0.0067 +1Score > 39 indicates identity
Score > 27 indicates homology		 U X X X R.SLPHVNVGTIGHVDHGK.T + Deamidated (NQ)
2860 +1 589.9860 1766.9362 1766.9064 16.8 0 33 0.0016 +1Score > 38 indicates identity
Score > 17 indicates homology		 U X X X R.SLPHVNVGTIGHVDHGK.T + Deamidated (NQ)
2862   590.2687 1767.7843 1767.7787 3.12 0 28 0.0056 +1Score > 39 indicates identity
Score > 18 indicates homology		 U X X X X X R.HYAHVDCPGHADYVK.N
2863   442.9535 1767.7849 1767.7787 3.48 0 7 1.1 +2Score > 39 indicates identity
Score > 20 indicates homology		 U X X X X X R.HYAHVDCPGHADYVK.N
2890   894.9758 1787.9370 1787.9166 11.4 1 46 0.00029 +1Score > 40 indicates identity
Score > 23 indicates homology		 U X R.GITINTAHVEYNSTIR.H
2894 +1 597.3160 1788.9262 1788.9006 14.3 1 55 0.0016 +1Score > 40 indicates identity U X R.GITINTAHVEYNSTIR.H + Deamidated (NQ)
2909   601.3134 1800.9184 1800.9370 -10.3 1 71 4.4e-005 +1Score > 40 indicates identity U X R.GITINTAHVEYNSLIR.H + Deamidated (NQ)
2909   601.3134 1800.9184 1800.9118 3.63 1 50 0.0056 +2Score > 40 indicates identity U X R.GITINTAHVEYNSNIR.H
2917 +1 601.6481 1801.9225 1801.8958 14.8 1 60 0.00058 +1Score > 40 indicates identity U X R.GITINTAHVEYNSNIR.H + Deamidated (NQ)
2919 +1 901.9708 1801.9270 1801.8958 17.3 1 86 1.3e-006 +1Score > 40 indicates identity U X R.GITINTAHVEYNSNIR.H + Deamidated (NQ)
2919 +1 901.9708 1801.9270 1801.9210 3.36 1 64 0.00019 +2Score > 40 indicates identity U X R.GITINTAHVEYNSLIR.H + 2 Deamidated (NQ)
2920 +1 601.6498 1801.9276 1801.9210 3.65 1 47 0.012 +2Score > 40 indicates identity U X R.GITINTAHVEYNSLIR.H + 2 Deamidated (NQ)
3432   712.3736 2134.0990 2134.0769 10.4 1 43 0.019 +1Score > 39 indicates identity U X X R.AIDQPFLMPIEDVFSISGR.G
3472   718.3835 2152.1287 2152.0874 19.2 1 24 0.033 +1Score > 38 indicates identity
Score > 22 indicates homology		 U X R.DIDKPFLMAIEDVFSISGR.G
3541 +1 1097.0704 2192.1262 2192.1551 -13.2 1 43 0.0036 +1Score > 38 indicates identity
Score > 31 indicates homology		 U X R.IIDKPFLMPIEDVFSISGR.G + Oxidation (M)
3547 +6 731.7192 2192.1358 2192.1551 -8.83 1 48 0.00015 +1Score > 38 indicates identity
Score > 22 indicates homology		 U X R.IIDKPFLMPIEDVFSISGR.G + Oxidation (M)

+61 subsets and intersections (678 subset proteins in total)

+2

Accession Score Description
1 ATPB_PSEPG 972 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPK 749 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
2 ATPA_RICAH 179 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
3 ATPA_VEREI 174 ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1

+4

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 584 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
3 CH602_SORC5 80 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1
2 CH601_ECOK1 123 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1

+5

Accession Score Description
1 DLDH2_PSEPU 541 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4

+6

Accession Score Description
1 RL1_PSEE4 419 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1

+7

Accession Score Description
1 EFG1_PSEPK 337 Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1

+8

Accession Score Description
1 RS5_PSEE4 331 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1

+9

Accession Score Description
Family member distances as a dendrogram 1 RL4_PSE14 327 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1
2 RL4_PSEE4 318 50S ribosomal protein L4 OS=Pseudomonas entomophila (strain L48) GN=rplD PE=3 SV=1

+10

Accession Score Description
1 RL18_PSEP1 310 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1
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