MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
Export

Not what you expected? Try the select summary.

Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

Filters

[help]

Show

Protein families 1–10 (out of 179)


Page: 1 2 3 4 5 6  18 Next 

+1

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1121 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
6 EFTU_CYAP7 40 Elongation factor Tu OS=Cyanothece sp. (strain PCC 7424) GN=tuf PE=3 SV=1
5 EFTU_ANATD 50 Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1
3 EFTU_PSEFS 701 Elongation factor Tu OS=Pseudomonas fluorescens (strain SBW25) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1002 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1
4 EFTU_CARRP 145 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1

+2

Accession Score Description
1 ATPB_PSEPG 972 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPK 749 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
2 ATPA_RICAH 179 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
3 ATPA_VEREI 174 ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1

+4

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 584 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
3 CH602_SORC5 80 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1
2 CH601_ECOK1 123 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1

+5

Accession Score Description
1 DLDH2_PSEPU 541 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4

-6

Accession Score Description
1 RL1_PSEE4 419 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1
Score Mass Matches Sequences emPAI
6.1 RL1_PSEE4 419 24208 13 (10) 6 (4) 0.73
50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1
2 samesets of RL1_PSEE4
RL1_PSEP1 419 24236 13 (10) 6 (4) 0.73
50S ribosomal protein L1 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplA PE=3 SV=1
RL1_PSEPK 419 24236 13 (10) 6 (4) 0.73
50S ribosomal protein L1 OS=Pseudomonas putida (strain KT2440) GN=rplA PE=3 SV=1

-13 peptide matches (9 non-duplicate, 4 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1300   342.5306 1024.5700 1024.5665 3.35 0 23 0.12 +1Score > 37 indicates identity
Score > 26 indicates homology
U K.NGIIHTSVGK.V
1386   356.5343 1066.5811 1066.5771 3.71 0 24 0.28 +1Score > 38 indicates identity
Score > 31 indicates homology
U R.SATVLPHGTGK.T
1725 -2 611.8713 1221.7280 1221.7194 7.10 0 62 1.1e-005 +1Score > 32 indicates identity
Score > 25 indicates homology
U R.VVGQLGQVLGPR.G
1724   611.8704 1221.7262 1221.7194 5.62 0 (50) 9.1e-005 +1Score > 32 indicates identity
Score > 23 indicates homology
U R.VVGQLGQVLGPR.G
1726   611.8724 1221.7302 1221.7194 8.90 0 (46) 0.00021 +1Score > 32 indicates identity
Score > 22 indicates homology
U R.VVGQLGQVLGPR.G
1799   629.3596 1256.7046 1256.6976 5.57 0 65 4.4e-005 +1Score > 39 indicates identity
Score > 34 indicates homology
U K.VGTVTPDVATAVK.N
2899 +1 897.4658 1792.9170 1792.8996 9.75 1 45 0.00078 +1Score > 40 indicates identity
Score > 26 indicates homology
U K.FVESYDIAVNLGVDPR.K
2900   598.6487 1792.9243 1792.8996 13.8 1 12 0.44 +1Score > 40 indicates identity
Score > 21 indicates homology
U K.FVESYDIAVNLGVDPR.K
3058   943.5013 1884.9880 1884.9694 9.91 1 128 1.3e-011 +1Score > 39 indicates identity
Score > 32 indicates homology
U R.VAVFTQGPAAEAALAAGADR.V
3060 +1 629.3385 1884.9937 1884.9694 12.9 1 84 6.2e-007 +1Score > 38 indicates identity
Score > 34 indicates homology
U R.VAVFTQGPAAEAALAAGADR.V
3061   944.0016 1885.9886 1885.9534 18.7 1 92 4.5e-008 +1Score > 39 indicates identity
Score > 31 indicates homology
U R.VAVFTQGPAAEAALAAGADR.V + Deamidated (NQ)

8 subsets and intersections (117 subset proteins in total)

Score Mass Subset of
RL1_PSEPW 394 24112 6.1
50S ribosomal protein L1 OS=Pseudomonas putida (strain W619) GN=rplA PE=3 SV=1
1 sameset of RL1_PSEPW
RL1_PSESM 394 24163
50S ribosomal protein L1 OS=Pseudomonas syringae pv. tomato GN=rplA PE=3 SV=1
RL1_PSEPG 388 24180 6.1
50S ribosomal protein L1 OS=Pseudomonas putida (strain GB-1) GN=rplA PE=3 SV=1
RL1_PSE14 363 24120 6.1
50S ribosomal protein L1 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplA PE=3 SV=1
2 samesets of RL1_PSE14
RL1_PSEF5 363 24047
50S ribosomal protein L1 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplA PE=3 SV=1
RL1_PSEU2 363 24176
50S ribosomal protein L1 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplA PE=3 SV=1
RL1_PSEFS 309 24061 6.1
50S ribosomal protein L1 OS=Pseudomonas fluorescens (strain SBW25) GN=rplA PE=3 SV=1
1 sameset of RL1_PSEFS
RL1_PSEPF 309 24193
50S ribosomal protein L1 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplA PE=3 SV=1
RL1_AZOVD 277 24230 6.1
50S ribosomal protein L1 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rplA PE=3 SV=1
RL1_PSEMY 145 23957 6.1
50S ribosomal protein L1 OS=Pseudomonas mendocina (strain ymp) GN=rplA PE=3 SV=1
1 sameset of RL1_PSEMY
RL1_PSEU5 145 24129
50S ribosomal protein L1 OS=Pseudomonas stutzeri (strain A1501) GN=rplA PE=3 SV=1
RL1_ACTP2 112 23932 6.1
50S ribosomal protein L1 OS=Actinobacillus pleuropneumoniae serotype 5b (strain L20) GN=rplA PE=3 SV=1
+58 samesets of RL1_ACTP2
RL1_ACIAC 65 23909 6.1
50S ribosomal protein L1 OS=Acidovorax avenae subsp. citrulli (strain AAC00-1) GN=rplA PE=3 SV=1
+46 samesets of RL1_ACIAC

+7

Accession Score Description
1 EFG1_PSEPK 337 Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1

+8

Accession Score Description
1 RS5_PSEE4 331 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1

+9

Accession Score Description
Family member distances as a dendrogram 1 RL4_PSE14 327 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1
2 RL4_PSEE4 318 50S ribosomal protein L4 OS=Pseudomonas entomophila (strain L48) GN=rplD PE=3 SV=1

+10

Accession Score Description
1 RL18_PSEP1 310 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1
Page: 1 2 3 4 5 6  18 Next 

Not what you expected? Try the select summary.