MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 179)

Page: 1 2 3 4 5 6 … 18 Next 

+1

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1121 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
6 EFTU_CYAP7 40 Elongation factor Tu OS=Cyanothece sp. (strain PCC 7424) GN=tuf PE=3 SV=1
5 EFTU_ANATD 50 Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1
3 EFTU_PSEFS 701 Elongation factor Tu OS=Pseudomonas fluorescens (strain SBW25) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1002 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1
4 EFTU_CARRP 145 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1

+2

Accession Score Description
1 ATPB_PSEPG 972 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPK 749 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
2 ATPA_RICAH 179 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
3 ATPA_VEREI 174 ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1

-4

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 584 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
3 CH602_SORC5 80 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1
2 CH601_ECOK1 123 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
CH60_PSEPK 584 56765 57 (27) 21 (11) 0.96
60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
CH601_ECOK1 123 57464 27 (9) 10 (4) 0.26
60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1
+27 samesets of CH601_ECOK1
CH602_SORC5 80 58067 7 (4) 3 (2) 0.12
60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1
1 sameset of CH602_SORC5
CH60_GEOLS 80 58655 11 (4) 4 (2) 0.12
60 kDa chaperonin OS=Geobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ) GN=groL PE=3 SV=1

-67 peptide matches (38 non-duplicate, 29 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 Peptide
212 +1 487.3283 486.3210 486.3166 9.15 0 33 0.85 +2Score > 46 indicates identity
Score > 45 indicates homology		 X X K.VAAVK.A
269   559.3836 558.3763 558.3741 4.01 0 20 0.19 +1Score > 34 indicates identity
Score > 25 indicates homology		 X R.VILSK.E
290 +1 586.3577 585.3504 585.3486 3.11 0 35 0.12 +1Score > 39 indicates identity X K.ATLGPK.G
310   602.3640 601.3567 601.3547 3.31 0 16 1.2 +5Score > 43 indicates identity
Score > 29 indicates homology		 X X K.ISNIR.E
311   301.6857 601.3568 601.3547 3.51 0 23 1.4 +4Score > 43 indicates identity
Score > 37 indicates homology		 X X K.ISNIR.E
380   322.2115 642.4084 642.4064 3.11 0 24 0.26 +1Score > 41 indicates identity
Score > 30 indicates homology		 U X R.NVVLAK.S
382   643.4160 642.4087 642.4064 3.54 0 39 0.086 +3Score > 41 indicates identity U X R.NVVLAK.S
395   652.3063 651.2990 651.2976 2.13 0 14 1.3 +4Score > 41 indicates identity
Score > 28 indicates homology		 X K.FGDSAR.K
396 +1 326.6573 651.3000 651.2976 3.70 0 32 0.094 +1Score > 40 indicates identity
Score > 35 indicates homology		 X K.FGDSAR.K
450 -1 675.3686 674.3613 674.3599 2.10 0 32 0.79 +1Score > 43 indicates identity
Score > 43 indicates homology		 X K.DGVSVAK.E
454   675.3792 674.3719 674.3599 17.8 0 (30) 0.94 +3Score > 42 indicates identity X K.DGVSVAK.E
451 +1 338.1882 674.3618 674.3599 2.88 0 23 0.19 +3Score > 44 indicates identity
Score > 28 indicates homology		 X K.DGVSVAK.E
552 +1 360.1785 718.3424 718.3398 3.63 0 29 0.11 +1Score > 43 indicates identity
Score > 32 indicates homology		 X X X K.APGFGDR.R
582   365.7353 729.4560 729.4497 8.71 1 16 1 +2Score > 36 indicates identity
Score > 28 indicates homology		 X X K.KISNIR.E
664 +1 386.2226 770.4306 770.4286 2.61 1 41 0.041 +1Score > 39 indicates identity U X R.AVESPLR.Q
665   771.4381 770.4308 770.4286 2.84 1 20 1.4 +2Score > 39 indicates identity
Score > 34 indicates homology		 U X R.AVESPLR.Q
778 +2 414.2726 826.5306 826.5276 3.65 0 38 0.037 +1Score > 36 indicates identity U X X K.LAGGVAVIK.V
779 +1 827.5380 826.5307 826.5276 3.74 0 46 0.005 +1Score > 36 indicates identity U X X K.LAGGVAVIK.V
1017   921.5058 920.4985 920.4967 1.94 0 46 0.016 +1Score > 40 indicates identity U X X K.SFGAPTITK.D
1019 +2 461.2573 920.5000 920.4967 3.60 0 32 0.14 +1Score > 40 indicates identity
Score > 37 indicates homology		 U X X K.SFGAPTITK.D
1039   927.5951 926.5878 926.5800 8.41 0 57 0.00028 +1Score > 34 indicates identity U X R.ALAAIIDLK.G
1040 +2 464.3022 926.5898 926.5800 10.6 0 55 0.00049 +1Score > 34 indicates identity U X R.ALAAIIDLK.G
1175   972.5756 971.5683 971.5651 3.29 1 66 0.00013 +1Score > 40 indicates identity U X K.ATAAVVAELK.N
1176 +3 486.7922 971.5698 971.5651 4.86 1 53 0.0014 +1Score > 40 indicates identity
Score > 37 indicates homology		 U X K.ATAAVVAELK.N
1268 +1 337.8465 1010.5177 1010.5145 3.14 1 44 0.0037 +1Score > 40 indicates identity
Score > 32 indicates homology		 U X X X R.VEDALHATR.A
1270   506.2671 1010.5196 1010.5145 5.10 1 61 0.00042 +1Score > 40 indicates identity U X X X R.VEDALHATR.A
1932   661.8289 1321.6432 1321.6336 7.28 1 16 0.31 +1Score > 41 indicates identity
Score > 23 indicates homology		 X K.DAFENMGAQLVK.E
1944 +1 664.8908 1327.7670 1327.7534 10.3 0 56 1.6e-005 +1Score > 36 indicates identity
Score > 21 indicates homology		 U X K.MLVGVNVLADAVK.A
2064 +1 693.4017 1384.7888 1384.7860 2.03 1 15 0.29 +1Score > 37 indicates identity
Score > 22 indicates homology		 U X K.MLRGVNVLADAVK.V
2130 +1 707.3122 1412.6098 1412.6055 3.05 1 48 0.0056 +1Score > 38 indicates identity U X R.AQIENTTSDYDR.E + Deamidated (NQ)
2323 +2 757.3914 1512.7682 1512.7532 9.93 1 99 6.8e-008 +1Score > 40 indicates identity U X K.GDNEDQNVGIALLR.R
2324 +1 505.2641 1512.7705 1512.7532 11.4 1 42 0.033 +1Score > 40 indicates identity U X K.GDNEDQNVGIALLR.R
2471 +2 790.9652 1579.9158 1579.9297 -8.80 1 8 18 +6Score > 33 indicates identity X R.GVNVLADAVKVTLGPK.G
2553   807.9029 1613.7912 1613.7897 0.98 0 71 2.1e-006 +1Score > 40 indicates identity
Score > 26 indicates homology		 U X R.QITANAGDEPSVVADK.V
2562   808.4050 1614.7954 1614.7737 13.5 0 73 7.6e-007 +1Score > 40 indicates identity
Score > 25 indicates homology		 U X R.QITANAGDEPSVVADK.V + Deamidated (NQ)
2982   614.9951 1841.9635 1841.9371 14.3 1 56 3.3e-005 +1Score > 39 indicates identity
Score > 23 indicates homology		 U X R.QITANAGDEPSVVADKVK.Q + Deamidated (NQ)
2983   614.9954 1841.9644 1841.9371 14.8 1 30 0.03 +1Score > 39 indicates identity
Score > 27 indicates homology		 U X R.QITANAGDEPSVVADKVK.Q + Deamidated (NQ)
3831 +1 801.4220 2401.2442 2401.1973 19.5 1 77 6.2e-006 +1Score > 37 indicates identity U X K.ANDAAGDGTTTATVLAQAIVNEGLK.A + Deamidated (NQ)
3831 +2 801.4220 2401.2442 2401.2336 4.38 1 63 0.00014 +4Score > 37 indicates identity U X K.ANDAAGDGTTTATVLAQAIITEGLK.A

+32 subsets and intersections (564 subset proteins in total)

+5

Accession Score Description
1 DLDH2_PSEPU 541 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4

+6

Accession Score Description
1 RL1_PSEE4 419 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1

+7

Accession Score Description
1 EFG1_PSEPK 337 Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1

+8

Accession Score Description
1 RS5_PSEE4 331 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1

+9

Accession Score Description
Family member distances as a dendrogram 1 RL4_PSE14 327 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1
2 RL4_PSEE4 318 50S ribosomal protein L4 OS=Pseudomonas entomophila (strain L48) GN=rplD PE=3 SV=1

+10

Accession Score Description
1 RL18_PSEP1 310 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1
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