MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 801–900 (out of 3698)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1812 439.8826 1316.6260 1316.6217 3.26 0 15 0.88 +1Score > 41 indicates identity
Score > 27 indicates homology
DLLGTNMMLHR + Deamidated (NQ); Oxidation (M)
4335 749.3744 2993.4685 2993.4294 13.1 1 15 0.054 +1Score > 37 indicates identity
Score > 15 indicates homology
YNVSVINGTSPFASDYDLTRIVAAYQE + Deamidated (NQ)
1940 692.8197 1383.6248 1383.6227 1.52 1 15 0.58 +1Score > 40 indicates identity
Score > 25 indicates homology
NAINENTAFLMK + 3 Deamidated (NQ); Oxidation (M)
3541 733.3655 2197.0747 2197.0434 14.2 0 15 0.54 +1Score > 39 indicates identity
Score > 25 indicates homology
DHTKPVQQVLADMTNGGVDR + Deamidated (NQ); Oxidation (M)
3928 835.0794 2502.2164 2502.2446 -11.3 1 15 0.15 +1Score > 38 indicates identity
Score > 19 indicates homology
TAQMGWSLARLIAQMGWGAPAMR
2844 901.9124 1801.8102 1801.8094 0.49 1 15 0.82 +1Score > 39 indicates identity
Score > 27 indicates homology
NGSSAKWMGPFYIGDR + Deamidated (NQ); Oxidation (M)
3371 706.3593 2116.0561 2116.0146 19.6 0 15 0.26 +1Score > 39 indicates identity
Score > 22 indicates homology
SVILWNIQDHITMAGSDSK + 2 Deamidated (NQ)
251 545.3220 544.3147 544.3081 12.1 1 15 0.94 +1Score > 44 indicates identity
Score > 27 indicates homology
KGNAR
3021 636.3368 1905.9886 1905.9605 14.7 1 15 0.64 +1Score > 39 indicates identity
Score > 26 indicates homology
VVLSDDDMALLKSIQNK + 2 Deamidated (NQ); Oxidation (M)
4226 955.1301 2862.3685 2862.3456 8.01 1 15 0.71 +1Score > 37 indicates identity
Score > 26 indicates homology
MAQLPPGDIATMPNQKVVFNAPFDNK + 4 Deamidated (NQ); Oxidation (M)
1082 482.2677 962.5208 962.5185 2.40 0 15 0.58 +1Score > 41 indicates identity
Score > 25 indicates homology
SPFVTSGIR
3038 638.9912 1913.9518 1913.9516 0.066 0 15 0.3 +1Score > 40 indicates identity
Score > 22 indicates homology
LLGDDNLAGQADQAAMLAK
670 795.4384 794.4311 794.4286 3.15 1 15 0.98 +1Score > 39 indicates identity
Score > 27 indicates homology
KQSYIR + Deamidated (NQ)
2159 498.5883 1492.7431 1492.7521 -6.07 1 15 0.99 +1Score > 41 indicates identity
Score > 27 indicates homology
QGTNVNHIEIPLR + 3 Deamidated (NQ)
969 463.2642 924.5138 924.5103 3.89 1 15 0.61 +1Score > 37 indicates identity
Score > 25 indicates homology
KMYSLGVK
3263 1030.5278 2059.0410 2059.0545 -6.54 1 15 0.33 +1Score > 39 indicates identity
Score > 23 indicates homology
IVSSQINSGNALSADVQKAR + 2 Deamidated (NQ)
1269 354.2078 1059.6016 1059.5938 7.38 0 15 0.26 +1Score > 39 indicates identity
Score > 22 indicates homology
GINIHLHTR
1717 422.9207 1265.7403 1265.7343 4.71 1 15 0.32 +1Score > 34 indicates identity
Score > 22 indicates homology
DLLPILEQVAR
3887 617.5836 2466.3053 2466.2867 7.56 1 15 0.13 +1Score > 36 indicates identity
Score > 18 indicates homology
QRVLSGVQPTGNLHLGNYLGAIR + 4 Deamidated (NQ)
1272 354.8474 1061.5204 1061.5175 2.68 1 15 0.84 +1Score > 42 indicates identity
Score > 27 indicates homology
VTCLENGLR + Deamidated (NQ)
1324 543.3165 1084.6184 1084.6240 -5.15 1 15 0.27 +1Score > 40 indicates identity
Score > 22 indicates homology
LLLLGAGESGR
1141 498.7800 995.5454 995.5400 5.49 0 15 0.47 +1Score > 38 indicates identity
Score > 24 indicates homology
VSDAPPALAR
1183 339.4989 1015.4749 1015.4934 -18.3 1 15 0.81 +1Score > 40 indicates identity
Score > 26 indicates homology
QVEDALQGR + Deamidated (NQ)
1064 319.8391 956.4955 956.4927 2.89 1 15 0.72 +1Score > 39 indicates identity
Score > 26 indicates homology
TPVAEDVAR
2909 919.9460 1837.8774 1837.8734 2.21 1 15 0.27 +1Score > 40 indicates identity
Score > 22 indicates homology
LENAGVNFDYLGAVNIE + Deamidated (NQ)
3264 1030.5303 2059.0460 2059.0545 -4.11 1 15 0.25 +1Score > 39 indicates identity
Score > 21 indicates homology
IVSSQINSGNALSADVQKAR + 2 Deamidated (NQ)
2379 796.4164 1590.8182 1590.8188 -0.37 1 15 0.2 +1Score > 40 indicates identity
Score > 20 indicates homology
DVGLRFDMTVPVAR + Oxidation (M)
849 437.7466 873.4786 873.4742 5.10 0 15 0.33 +1Score > 42 indicates identity
Score > 22 indicates homology
LQCLAAAK
1551 591.3495 1180.6844 1180.7067 -18.8 1 15 0.79 +1Score > 35 indicates identity
Score > 26 indicates homology
LLLNPAIKAVE + Deamidated (NQ)
1607 606.3555 1210.6964 1210.6809 12.8 1 15 0.74 +1Score > 36 indicates identity
Score > 26 indicates homology
VIVPEAITIQE
3836 802.7262 2405.1568 2405.1097 19.6 1 15 0.73 +1Score > 39 indicates identity
Score > 26 indicates homology
FSNSFLMDGNTNFGIQILQKE + 3 Deamidated (NQ)
1259 528.7951 1055.5756 1055.5723 3.15 1 15 0.2 +1Score > 39 indicates identity
Score > 20 indicates homology
KLAAQPSDAR
3271 1030.5413 2059.0680 2059.0545 6.57 1 15 0.36 +1Score > 38 indicates identity
Score > 23 indicates homology
IVSSQINSGNALSADVQKAR + 2 Deamidated (NQ)
1956 463.5959 1387.7659 1387.7605 3.84 1 15 0.61 +1Score > 38 indicates identity
Score > 25 indicates homology
MLIQRATNVSVR + Deamidated (NQ)
1989 468.9308 1403.7706 1403.7772 -4.74 1 15 0.38 +1Score > 38 indicates identity
Score > 23 indicates homology
LLYNNIKDNGLK
2701 866.8888 1731.7630 1731.7807 -10.2 0 15 0.24 +1Score > 38 indicates identity
Score > 21 indicates homology
NDNPLVDMQAMSPTAK + Deamidated (NQ)
1430 566.7999 1131.5852 1131.6070 -19.2 1 15 0.27 +1Score > 42 indicates identity
Score > 21 indicates homology
NLGRVMAQVK + Deamidated (NQ); Oxidation (M)
1714 632.8160 1263.6174 1263.6207 -2.61 1 15 0.96 +1Score > 41 indicates identity
Score > 27 indicates homology
SGQALRYTDPR + Deamidated (NQ)
2529 551.9561 1652.8465 1652.8668 -12.3 1 15 0.74 +1Score > 40 indicates identity
Score > 26 indicates homology
AHADRNIVIMLTGNK + Deamidated (NQ)
2145 742.4144 1482.8142 1482.8195 -3.51 1 15 0.14 +1Score > 38 indicates identity
Score > 19 indicates homology
LVPKGQTPTSWLR + Deamidated (NQ)
2181 750.4451 1498.8756 1498.8541 14.4 1 15 0.98 +1Score > 33 indicates identity
Score > 27 indicates homology
NKLLIIDGMNLVR + Deamidated (NQ)
2414 537.5947 1609.7623 1609.7624 -0.073 1 14 0.43 +1Score > 40 indicates identity
Score > 23 indicates homology
LVGANNFYVGVEQAE + Deamidated (NQ)
2411 537.3190 1608.9352 1608.9199 9.49 0 14 0.094 +1Score > 33 indicates identity
Score > 17 indicates homology
VQPQVVTILADSAIR
3355 704.3667 2110.0783 2110.0921 -6.55 1 14 0.74 +1Score > 39 indicates identity
Score > 26 indicates homology
LLFNDIIFRMPNPNYVK + Deamidated (NQ); Oxidation (M)
1090 485.7657 969.5168 969.5019 15.5 1 14 0.41 +1Score > 39 indicates identity
Score > 23 indicates homology
TLPAPTEIE
1222 518.2962 1034.5778 1034.5760 1.78 1 14 0.81 +1Score > 38 indicates identity
Score > 26 indicates homology
IFAKNNISK + Deamidated (NQ)
2281 515.9508 1544.8306 1544.8106 12.9 1 14 0.49 +1Score > 39 indicates identity
Score > 24 indicates homology
HVCRHAAVALGQAR
857 440.2381 878.4616 878.4497 13.6 1 14 0.99 +1Score > 41 indicates identity
Score > 27 indicates homology
YNAEAIAK
2972 936.9677 1871.9208 1871.9523 -16.8 1 14 0.89 +1Score > 40 indicates identity
Score > 26 indicates homology
DRLMTLQQPISAAANAR + Deamidated (NQ); Oxidation (M)
2399 536.2835 1605.8287 1605.7998 18.0 1 14 0.5 +1Score > 40 indicates identity
Score > 24 indicates homology
TLFNGTIALAGRDQE + Deamidated (NQ)
2953 932.9931 1863.9716 1863.9941 -12.1 1 14 0.82 +1Score > 39 indicates identity
Score > 26 indicates homology
INTTSLNLYQLRDAIK + 2 Deamidated (NQ)
2254 765.4324 1528.8502 1528.8712 -13.7 1 14 0.67 +1Score > 37 indicates identity
Score > 25 indicates homology
LQTAITLIDELTAK
2614 846.4664 1690.9182 1690.9002 10.7 1 14 0.69 +1Score > 37 indicates identity
Score > 25 indicates homology
GVRVNIIHQAVGAINE + 2 Deamidated (NQ)
1857 672.3625 1342.7104 1342.7092 0.90 1 14 0.53 +1Score > 41 indicates identity
Score > 24 indicates homology
VVDLVQQATNEK
1617 405.8919 1214.6539 1214.6619 -6.59 1 14 0.99 +1Score > 41 indicates identity
Score > 27 indicates homology
GSKILVQQGQR + 2 Deamidated (NQ)
1136 498.3195 994.6244 994.6175 6.99 1 14 0.45 +1Score > 30 indicates identity
Score > 23 indicates homology
IKKPSTPPK
1746 639.8353 1277.6560 1277.6438 9.59 0 14 1 +1Score > 41 indicates identity
Score > 27 indicates homology
LLDIYPMQGGR + Oxidation (M)
3473 721.7141 2162.1205 2162.1214 -0.44 1 14 0.9 +1Score > 38 indicates identity
Score > 26 indicates homology
LISSSLCLLTDQVIEMVIE
3759 780.4036 2338.1890 2338.2290 -17.1 1 14 0.28 +1Score > 38 indicates identity
Score > 21 indicates homology
HIHFIGMGGIGMSALALILAER + 2 Oxidation (M)
2677 574.2905 1719.8497 1719.8726 -13.3 0 14 0.84 +1Score > 41 indicates identity
Score > 26 indicates homology
GQIPQHIAIIMDGNGR + Deamidated (NQ)
1180 507.8122 1013.6098 1013.6233 -13.3 0 14 0.76 +1Score > 37 indicates identity
Score > 26 indicates homology
LTATINILR
3063 967.5350 1933.0554 1933.0884 -17.1 0 14 0.58 +1Score > 36 indicates identity
Score > 24 indicates homology
QASAVIQIDLLDHVIIGK + Deamidated (NQ)
3290 692.7137 2075.1193 2075.0899 14.2 1 14 0.084 +1Score > 36 indicates identity
Score > 16 indicates homology
ATDLDVFLKGGAALDINSVR + Deamidated (NQ)
3103 985.9932 1969.9718 1969.9381 17.1 1 14 0.24 +1Score > 40 indicates identity
Score > 20 indicates homology
APEPALEPESLDFSGAGQR
3659 761.7550 2282.2432 2282.2053 16.6 1 14 0.2 +1Score > 35 indicates identity
Score > 20 indicates homology
GQSPLKMTSSPVLPVGSAINGSR
2037 475.5775 1423.7107 1423.7381 -19.3 0 14 0.83 +1Score > 41 indicates identity
Score > 26 indicates homology
MISVAINGYGTIGK + Deamidated (NQ)
2613 564.6375 1690.8907 1690.9123 -12.8 1 14 0.61 +1Score > 39 indicates identity
Score > 24 indicates homology
HLRMMHLAATLIQR + Deamidated (NQ)
2019 474.2579 1419.7519 1419.7432 6.12 0 14 0.35 +1Score > 40 indicates identity
Score > 22 indicates homology
GIMLQTLDSLWK + Oxidation (M)
2449 540.6334 1618.8784 1618.8687 5.97 0 14 0.5 +1Score > 38 indicates identity
Score > 24 indicates homology
VGLHNMPPLMLAGLR + Deamidated (NQ)
700 406.7358 811.4570 811.4664 -11.6 1 14 0.99 +1Score > 37 indicates identity
Score > 27 indicates homology
IRVDGPR
3272 515.7759 2059.0745 2059.0983 -11.6 1 14 0.42 +1Score > 38 indicates identity
Score > 23 indicates homology
ALAQTLLSTAVNNMLVRDK + 2 Deamidated (NQ)
1532 586.8530 1171.6914 1171.6924 -0.85 1 14 0.65 +1Score > 36 indicates identity
Score > 25 indicates homology
QATKLTAGIAAK
3714 769.7081 2306.1025 2306.1464 -19.0 1 14 0.18 +1Score > 39 indicates identity
Score > 19 indicates homology
FQSDLKIINDCLDGLIQNAK + 2 Deamidated (NQ)
1980 701.3933 1400.7720 1400.7736 -1.08 1 14 0.56 +1Score > 39 indicates identity
Score > 24 indicates homology
QSLSSGGAKVALQR
3308 696.0094 2085.0064 2085.0010 2.60 1 14 0.097 +1Score > 39 indicates identity
Score > 16 indicates homology
ILCAYASLVSQDVEMLQK + 2 Deamidated (NQ); Oxidation (M)
1456 571.8615 1141.7084 1141.7070 1.23 0 14 0.93 +1Score > 31 indicates identity
Score > 26 indicates homology
ISIQVLQTLK
1109 488.7802 975.5458 975.5641 -18.7 0 14 0.66 +1Score > 40 indicates identity
Score > 25 indicates homology
ILVYLVQE
3068 646.6987 1937.0743 1937.0768 -1.32 0 14 0.4 +1Score > 35 indicates identity
Score > 22 indicates homology
DAIIVGCAQVLALVPGVSR
859 440.2526 878.4906 878.4862 5.11 1 14 0.65 +1Score > 38 indicates identity
Score > 25 indicates homology
IETVVYR
1777 647.9079 1293.8012 1293.7908 8.10 1 14 0.8 +1Score > 29 indicates identity
Score > 25 indicates homology
EVVNVPIIGLIK + Deamidated (NQ)
1274 354.8584 1061.5534 1061.5539 -0.49 0 14 0.3 +1Score > 41 indicates identity
Score > 21 indicates homology
MVALSNALSR + Deamidated (NQ)
2278 772.4261 1542.8376 1542.8439 -4.06 0 14 0.78 +1Score > 39 indicates identity
Score > 25 indicates homology
NMVVSNILLNNIAK + Deamidated (NQ)
1471 1151.5632 1150.5559 1150.5731 -14.9 0 14 0.48 +1Score > 41 indicates identity
Score > 23 indicates homology
GAPTGHDINIR + Deamidated (NQ)
3545 735.0603 2202.1591 2202.1354 10.7 1 14 0.13 +1Score > 37 indicates identity
Score > 18 indicates homology
IMDYSLLLGIHVLNSNVRE + Deamidated (NQ); Oxidation (M)
2098 732.3906 1462.7666 1462.7569 6.70 0 14 0.56 +1Score > 40 indicates identity
Score > 24 indicates homology
TDALQGYQWLLR
2691 864.4684 1726.9222 1726.9326 -5.97 1 14 0.81 +1Score > 38 indicates identity
Score > 25 indicates homology
SVIAGDAKPSQAIRASR + Deamidated (NQ)
2217 755.9464 1509.8782 1509.8654 8.51 0 14 0.43 +1Score > 33 indicates identity
Score > 23 indicates homology
LIVLTDTPGILLNE
2548 417.2343 1664.9081 1664.9138 -3.42 1 14 0.31 +1Score > 37 indicates identity
Score > 21 indicates homology
TVLGFFGITDIEVVR
358 321.2032 640.3918 640.3908 1.65 0 14 0.87 +1Score > 36 indicates identity
Score > 26 indicates homology
KPATPK
2070 722.4033 1442.7920 1442.7841 5.50 1 14 0.24 +1Score > 38 indicates identity
Score > 20 indicates homology
GNQAIQRSIDTIK
2722 870.4727 1738.9308 1738.9011 17.1 1 14 0.53 +1Score > 38 indicates identity
Score > 24 indicates homology
INAGMYIFSPAMLRR
2755 587.3571 1759.0495 1759.0145 19.9 1 14 0.31 +1Score > 26 indicates identity
Score > 21 indicates homology
AGFVIVAIPPAHRVAIE
3287 692.6864 2075.0374 2075.0034 16.4 1 14 0.55 +1Score > 40 indicates identity
Score > 24 indicates homology
WNLIWIDLEMTGLDPSR + Deamidated (NQ); Oxidation (M)
929 454.2021 906.3896 906.4018 -13.4 0 14 0.56 +1Score > 39 indicates identity
Score > 24 indicates homology
DGYIHMR + Oxidation (M)
2878 907.4584 1812.9022 1812.8853 9.33 0 14 0.35 +1Score > 40 indicates identity
Score > 22 indicates homology
TNNNVSLGLADLAGAEPR + 2 Deamidated (NQ)
2690 576.6255 1726.8547 1726.8672 -7.27 0 14 0.38 +1Score > 40 indicates identity
Score > 22 indicates homology
LPVSMPSGNQQPSLTR + Oxidation (M)
1467 384.1887 1149.5443 1149.5410 2.89 1 14 0.3 +1Score > 42 indicates identity
Score > 21 indicates homology
KAGMIPLQME + Deamidated (NQ); 2 Oxidation (M)
2912 920.0046 1837.9946 1837.9897 2.68 1 14 0.076 +1Score > 37 indicates identity
Score > 15 indicates homology
AQLVAEALSPGANVSAIAR + Deamidated (NQ)
1533 586.8536 1171.6926 1171.6812 9.76 1 14 0.87 +1Score > 36 indicates identity
Score > 26 indicates homology
KLQQSVDLLK + Deamidated (NQ)
3856 812.4413 2434.3021 2434.3431 -16.9 1 14 0.69 +1Score > 36 indicates identity
Score > 25 indicates homology
VSDAVQRAILADLPDIIISTPAR + Deamidated (NQ)
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