MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 701–800 (out of 3698)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3140 499.7463 1994.9561 1994.9561 0.021 1 16 0.77 +1Score > 40 indicates identity
Score > 27 indicates homology
FMSQPFHVAEIFTGSPGK + Oxidation (M)
365 322.7088 643.4030 643.4017 2.11 1 16 0.33 +1Score > 44 indicates identity
Score > 24 indicates homology
VQIKR + Deamidated (NQ)
1050 317.8356 950.4850 950.4709 14.8 1 16 0.95 +1Score > 41 indicates identity
Score > 28 indicates homology
FENSGVLGK + Deamidated (NQ)
2421 538.2713 1611.7921 1611.7933 -0.74 0 16 0.23 +1Score > 40 indicates identity
Score > 22 indicates homology
ANFLAISGYIWNNK + 2 Deamidated (NQ)
2301 519.6237 1555.8493 1555.8392 6.49 1 16 0.56 +1Score > 38 indicates identity
Score > 26 indicates homology
AMALLENIDIQIGR
2371 794.4307 1586.8468 1586.8529 -3.80 1 16 0.68 +1Score > 40 indicates identity
Score > 27 indicates homology
LSLTVLGGNRQWSR + Deamidated (NQ)
2732 582.0013 1742.9821 1742.9778 2.45 1 16 0.13 +1Score > 35 indicates identity
Score > 20 indicates homology
TGITAIDALNTLVKGQK + Deamidated (NQ)
1236 521.7788 1041.5430 1041.5342 8.47 1 16 0.4 +1Score > 40 indicates identity
Score > 24 indicates homology
VSLSPGPEQK + Deamidated (NQ)
1983 702.8665 1403.7184 1403.6946 17.0 1 16 0.76 +1Score > 41 indicates identity
Score > 27 indicates homology
LHNIRDFAFDR + Deamidated (NQ)
1908 456.5699 1366.6879 1366.6915 -2.64 0 16 0.84 +1Score > 40 indicates identity
Score > 28 indicates homology
GTYVCIATNALGK
2409 804.8983 1607.7820 1607.7613 12.9 1 16 0.29 +1Score > 41 indicates identity
Score > 23 indicates homology
LQNNGYIAKVQCAE + Deamidated (NQ)
415 337.2037 672.3928 672.3919 1.47 0 16 0.71 +1Score > 43 indicates identity
Score > 27 indicates homology
AVSLQR
2955 622.6379 1864.8919 1864.8724 10.4 1 16 0.22 +1Score > 40 indicates identity
Score > 22 indicates homology
NTDLMATVDSILNDEAK + Oxidation (M)
3053 963.4724 1924.9302 1924.8949 18.4 1 16 0.11 +1Score > 40 indicates identity
Score > 19 indicates homology
DQLKMNNNDYIQSVAR + Deamidated (NQ); Oxidation (M)
3787 788.7228 2363.1466 2363.1129 14.3 1 16 0.16 +1Score > 39 indicates identity
Score > 20 indicates homology
TVAGPGEGTVAPTALQGPSPGSPGQE + 2 Deamidated (NQ)
971 464.2495 926.4844 926.4709 14.6 0 16 0.66 +1Score > 39 indicates identity
Score > 27 indicates homology
ALQGVPVNE + Deamidated (NQ)
1270 530.8192 1059.6238 1059.6077 15.3 0 16 0.26 +1Score > 36 indicates identity
Score > 23 indicates homology
GLDIFNLIR
668 398.1992 794.3838 794.3810 3.57 1 16 0.59 +1Score > 41 indicates identity
Score > 26 indicates homology
AVFENSK + Deamidated (NQ)
890 447.7255 893.4364 893.4243 13.6 0 16 0.87 +1Score > 42 indicates identity
Score > 28 indicates homology
NVAFNTAR + 2 Deamidated (NQ)
1821 660.3836 1318.7526 1318.7497 2.26 1 16 0.76 +1Score > 35 indicates identity
Score > 27 indicates homology
FLGTTKDPVTLK
2233 759.3983 1516.7820 1516.7773 3.12 0 16 0.25 +1Score > 41 indicates identity
Score > 22 indicates homology
YIAQALPQDVLGTE
1248 349.8416 1046.5030 1046.5107 -7.34 1 16 0.47 +1Score > 41 indicates identity
Score > 25 indicates homology
KMTYVDYK
2859 904.4568 1806.8990 1806.9220 -12.7 0 16 0.45 +1Score > 40 indicates identity
Score > 25 indicates homology
INIVVLGSMNTDLVMR + Deamidated (NQ); 2 Oxidation (M)
2275 772.4072 1542.7998 1542.8140 -9.20 0 16 0.3 +1Score > 40 indicates identity
Score > 23 indicates homology
LAIQNSQIASAIALE + 2 Deamidated (NQ)
3205 1012.5289 2023.0432 2023.0698 -13.1 1 16 0.11 +1Score > 39 indicates identity
Score > 19 indicates homology
SAALQSIQENLGLQDAPLR
3762 586.0218 2340.0581 2340.0811 -9.83 1 16 0.51 +1Score > 37 indicates identity
Score > 25 indicates homology
ADYVKAFWNVVNWDDVQNR + 2 Deamidated (NQ)
1491 386.5325 1156.5757 1156.5836 -6.88 1 16 0.58 +1Score > 40 indicates identity
Score > 26 indicates homology
GAELQQAGIDR
2519 825.9310 1649.8474 1649.8334 8.49 1 16 0.23 +1Score > 40 indicates identity
Score > 22 indicates homology
AVLLMAAFELTQQAE + Oxidation (M)
520 363.7015 725.3884 725.3894 -1.32 1 16 0.9 +1Score > 37 indicates identity
Score > 28 indicates homology
YIMKR + Oxidation (M)
1640 409.8801 1226.6185 1226.6044 11.5 0 16 0.55 +1Score > 40 indicates identity
Score > 26 indicates homology
NEPDIWNAIR
1862 449.8711 1346.5915 1346.5772 10.6 1 16 0.24 +1Score > 38 indicates identity
Score > 22 indicates homology
QSQENHISQMK + 2 Deamidated (NQ); Oxidation (M)
2167 748.8729 1495.7312 1495.7129 12.2 0 16 0.6 +1Score > 41 indicates identity
Score > 26 indicates homology
FMIAANGFEPDLR + Oxidation (M)
513 361.7017 721.3888 721.3793 13.3 0 16 0.64 +1Score > 42 indicates identity
Score > 26 indicates homology
MSTIVR + Oxidation (M)
3248 682.7134 2045.1184 2045.1044 6.82 1 16 0.29 +1Score > 35 indicates identity
Score > 23 indicates homology
LAKNGAAFISVVSSNPDLLK + 2 Deamidated (NQ)
936 456.7625 911.5104 911.5116 -1.31 1 16 0.93 +1Score > 38 indicates identity
Score > 28 indicates homology
KIVDYFK
1276 532.2771 1062.5396 1062.5458 -5.78 1 16 0.61 +1Score > 42 indicates identity
Score > 26 indicates homology
ALFGAARDDK
328 311.6593 621.3040 621.2970 11.4 0 16 0.97 +1Score > 40 indicates identity
Score > 28 indicates homology
ADSTTK
2180 500.6306 1498.8700 1498.8541 10.6 1 16 0.42 +1Score > 33 indicates identity
Score > 24 indicates homology
NKLLIIDGMNLVR + Deamidated (NQ)
3686 1150.0837 2298.1528 2298.1300 9.93 1 16 0.18 +1Score > 39 indicates identity
Score > 21 indicates homology
NPENLINDAPIKPTLIGMSNK + 4 Deamidated (NQ); Oxidation (M)
2556 556.9429 1667.8069 1667.8076 -0.43 1 16 0.52 +1Score > 40 indicates identity
Score > 25 indicates homology
IMQLADKANQYIDK + 2 Deamidated (NQ); Oxidation (M)
1895 683.3215 1364.6284 1364.6030 18.6 1 16 0.68 +1Score > 40 indicates identity
Score > 26 indicates homology
ANEQLGFPADMR + Deamidated (NQ); Oxidation (M)
284 586.3864 585.3791 585.3737 9.18 0 16 0.6 +1Score > 39 indicates identity
Score > 26 indicates homology
ILDIL
1157 501.7843 1001.5540 1001.5506 3.49 0 16 0.96 +1Score > 41 indicates identity
Score > 28 indicates homology
TQDAVSLLR
614 387.7410 773.4674 773.4647 3.58 1 16 0.95 +1Score > 38 indicates identity
Score > 28 indicates homology
ISADKIK
618 388.2324 774.4502 774.4487 2.00 0 16 0.82 +1Score > 40 indicates identity
Score > 27 indicates homology
ISDSILK
2938 928.4540 1854.8934 1854.9199 -14.3 1 16 0.29 +1Score > 40 indicates identity
Score > 23 indicates homology
AFAQAGWKTICAFQTR
552 369.1961 736.3776 736.3868 -12.4 1 16 0.97 +1Score > 42 indicates identity
Score > 28 indicates homology
KNHPLE
1257 528.2866 1054.5586 1054.5447 13.2 0 16 0.35 +1Score > 39 indicates identity
Score > 23 indicates homology
LGGYLYDVR
3422 714.3981 2140.1725 2140.1582 6.68 1 16 0.16 +1Score > 35 indicates identity
Score > 20 indicates homology
LHWYRSPLPFATDGVILR
2515 825.9286 1649.8426 1649.8426 0.0073 1 15 0.77 +1Score > 40 indicates identity
Score > 27 indicates homology
ITWWHKLDPANNR
841 435.7198 869.4250 869.4243 0.86 0 15 0.63 +1Score > 39 indicates identity
Score > 26 indicates homology
GLVSHADAT
1198 343.1928 1026.5566 1026.5498 6.56 1 15 0.76 +1Score > 39 indicates identity
Score > 27 indicates homology
RVYDTFVK
2186 752.3769 1502.7392 1502.7154 15.9 0 15 0.84 +1Score > 41 indicates identity
Score > 27 indicates homology
HYWNIGIAADTDK
1669 618.3076 1234.6006 1234.5975 2.51 1 15 0.25 +1Score > 42 indicates identity
Score > 22 indicates homology
KSAMQAGSDLAR + Deamidated (NQ)
4362 1016.8969 3047.6689 3047.6179 16.7 1 15 1 +1Score > 31 indicates identity
Score > 28 indicates homology
ATPIAVVPFGLQGGSVLPEDIADIIGNDLR + Deamidated (NQ)
1631 611.3817 1220.7488 1220.7492 -0.32 0 15 0.089 +1Score > 27 indicates identity
Score > 17 indicates homology
QLIPPLLTGLR + Deamidated (NQ)
259 557.3675 556.3602 556.3585 3.17 0 15 0.31 +1Score > 37 indicates identity
Score > 23 indicates homology
VVVVAA
553 369.2024 736.3902 736.3901 0.15 1 15 0.71 +1Score > 41 indicates identity
Score > 26 indicates homology
LSKSMR + Oxidation (M)
1211 516.7448 1031.4750 1031.4746 0.43 1 15 0.68 +1Score > 40 indicates identity
Score > 26 indicates homology
MKFQDGYK + Oxidation (M)
3436 1073.5919 2145.1692 2145.1317 17.5 1 15 0.3 +1Score > 35 indicates identity
Score > 23 indicates homology
DLSGSIASPDVKLNLGGDFIK
1139 332.8548 995.5426 995.5512 -8.69 1 15 0.73 +1Score > 37 indicates identity
Score > 27 indicates homology
DASVPPVRR
1531 586.8517 1171.6888 1171.6812 6.52 1 15 0.55 +1Score > 36 indicates identity
Score > 25 indicates homology
LTLKLQTLNE
3267 1030.5325 2059.0504 2059.0884 -18.5 1 15 0.91 +1Score > 39 indicates identity
Score > 27 indicates homology
ANLVFGGTNLQIVMVPGRR + 2 Deamidated (NQ); Oxidation (M)
1961 463.9343 1388.7811 1388.7697 8.18 1 15 0.55 +1Score > 37 indicates identity
Score > 25 indicates homology
AIKQSDVVMLLR + Deamidated (NQ); Oxidation (M)
1871 676.8508 1351.6870 1351.6884 -1.01 1 15 0.68 +1Score > 41 indicates identity
Score > 26 indicates homology
LYQQRDPIYR + Deamidated (NQ)
541 366.7067 731.3988 731.3926 8.57 1 15 0.87 +1Score > 41 indicates identity
Score > 27 indicates homology
KDAATAR
1367 554.2518 1106.4890 1106.4914 -2.09 1 15 0.86 +1Score > 40 indicates identity
Score > 27 indicates homology
VLDQMNENK + Deamidated (NQ); Oxidation (M)
2714 578.6542 1732.9408 1732.9216 11.1 1 15 0.49 +1Score > 38 indicates identity
Score > 25 indicates homology
VLDLGCGAGVIGSMIKK + Oxidation (M)
3077 650.9963 1949.9671 1949.9735 -3.27 1 15 0.22 +1Score > 40 indicates identity
Score > 21 indicates homology
VLADIQQGRFALDFVQE + 2 Deamidated (NQ)
3505 545.7712 2179.0557 2179.0983 -19.6 1 15 0.8 +1Score > 39 indicates identity
Score > 27 indicates homology
YINPGDMLIVNNTKVFPAR + 2 Deamidated (NQ); Oxidation (M)
1056 478.2596 954.5046 954.5208 -16.9 0 15 0.76 +1Score > 38 indicates identity
Score > 27 indicates homology
MLGLAEPPK
3333 1050.0485 2098.0824 2098.0841 -0.78 1 15 0.35 +1Score > 38 indicates identity
Score > 23 indicates homology
KTVQNALSDLGMNIVSVHR + Deamidated (NQ); Oxidation (M)
1340 365.5255 1093.5547 1093.5503 4.00 1 15 0.72 +1Score > 41 indicates identity
Score > 26 indicates homology
TDKVLSTTTE
2182 750.4489 1498.8832 1498.8541 19.5 1 15 0.23 +1Score > 32 indicates identity
Score > 21 indicates homology
NKLLIIDGMNLVR + Deamidated (NQ)
2466 816.4126 1630.8106 1630.8355 -15.2 1 15 0.75 +1Score > 41 indicates identity
Score > 27 indicates homology
YFTPALTTVHQPKE
3507 728.3957 2182.1653 2182.1773 -5.50 0 15 0.17 +1Score > 36 indicates identity
Score > 20 indicates homology
FALSLLVLLAGLPTLDANDPE + Deamidated (NQ)
2279 515.2868 1542.8386 1542.8504 -7.70 1 15 0.85 +1Score > 39 indicates identity
Score > 27 indicates homology
LAATTSQAELLALIE
3665 763.4335 2287.2787 2287.2827 -1.78 0 15 0.073 +1Score > 32 indicates identity
Score > 16 indicates homology
VLFLQGGASLQFSAIPLNLLGK + 2 Deamidated (NQ)
2031 712.3643 1422.7140 1422.7242 -7.11 1 15 0.84 +1Score > 41 indicates identity
Score > 27 indicates homology
LNLINSTYKNLE + 2 Deamidated (NQ)
2574 557.3348 1668.9826 1668.9563 15.8 1 15 0.48 +1Score > 31 indicates identity
Score > 24 indicates homology
SRLPLLDLNIYVPR + Deamidated (NQ)
2496 820.9165 1639.8184 1639.8279 -5.79 0 15 0.32 +1Score > 40 indicates identity
Score > 23 indicates homology
WIAYAQSLTSKPMK + Deamidated (NQ); Oxidation (M)
3284 692.3541 2074.0405 2074.0378 1.29 1 15 0.76 +1Score > 39 indicates identity
Score > 27 indicates homology
NLGQAMPGVSNLDVRFSNR
2115 491.9185 1472.7337 1472.7293 2.97 0 15 0.67 +1Score > 41 indicates identity
Score > 26 indicates homology
NNLLSCALPDSIR + Deamidated (NQ)
3180 671.3525 2011.0357 2011.0262 4.71 1 15 0.61 +1Score > 39 indicates identity
Score > 25 indicates homology
QTLSPSVLIEHLNLFGNE + Deamidated (NQ)
973 464.2735 926.5324 926.5185 15.0 0 15 0.99 +1Score > 38 indicates identity
Score > 28 indicates homology
LASSPSPLR
3178 671.3513 2011.0321 2011.0262 2.92 1 15 0.4 +1Score > 39 indicates identity
Score > 24 indicates homology
QTLSPSVLIEHLNLFGNE + Deamidated (NQ)
2806 593.6108 1777.8106 1777.7842 14.8 0 15 0.83 +1Score > 39 indicates identity
Score > 27 indicates homology
VQIWSCSGGANQQWR + 2 Deamidated (NQ)
3071 972.5194 1943.0242 1942.9863 19.5 1 15 0.13 +1Score > 38 indicates identity
Score > 19 indicates homology
VFVMDQAESFLPLYLR + Oxidation (M)
2526 826.4458 1650.8770 1650.8464 18.6 1 15 0.28 +1Score > 39 indicates identity
Score > 22 indicates homology
QIQEQHQAAIIIQK + 4 Deamidated (NQ)
3662 762.3912 2284.1518 2284.1587 -3.02 1 15 0.2 +1Score > 39 indicates identity
Score > 21 indicates homology
DFSINIISKSGTTTEPSIAFR + Deamidated (NQ)
1088 322.8513 965.5321 965.5294 2.76 0 15 0.44 +1Score > 38 indicates identity
Score > 24 indicates homology
QPILAGNPR + Deamidated (NQ)
1966 695.8781 1389.7416 1389.7173 17.5 0 15 0.71 +1Score > 40 indicates identity
Score > 26 indicates homology
NVVMTGNLLLNGK + 2 Deamidated (NQ); Oxidation (M)
3051 642.3169 1923.9289 1923.9361 -3.73 1 15 0.31 +1Score > 40 indicates identity
Score > 22 indicates homology
DGTVRYDMTDLPVTAVR + Oxidation (M)
2831 600.3364 1797.9874 1797.9948 -4.13 1 15 0.44 +1Score > 36 indicates identity
Score > 24 indicates homology
ADTAAKNQALSLIAAAIR + Deamidated (NQ)
2510 550.9413 1649.8021 1649.7831 11.5 1 15 0.16 +1Score > 41 indicates identity
Score > 20 indicates homology
QVLENHPDAPMDLR + Oxidation (M)
569 749.4335 748.4262 748.4232 4.08 0 15 0.85 +1Score > 40 indicates identity
Score > 27 indicates homology
AVFSGIR
1386 373.1953 1116.5641 1116.5775 -12.0 0 15 0.4 +1Score > 42 indicates identity
Score > 24 indicates homology
INSLGTLQDR + Deamidated (NQ)
1626 610.3284 1218.6422 1218.6390 2.66 1 15 0.88 +1Score > 41 indicates identity
Score > 27 indicates homology
AALASMQRLNK + Deamidated (NQ); Oxidation (M)
2269 769.9129 1537.8112 1537.8174 -3.99 0 15 0.69 +1Score > 39 indicates identity
Score > 26 indicates homology
LSNLSISLMIQFR + Deamidated (NQ); Oxidation (M)
1359 552.8299 1103.6452 1103.6260 17.4 1 15 0.6 +1Score > 36 indicates identity
Score > 25 indicates homology
KVVLLGMSLE + Oxidation (M)
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