MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 601–700 (out of 3698)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1063 319.8390 956.4952 956.5113 -16.9 0 17 0.72 +1Score > 39 indicates identity
Score > 28 indicates homology
LCLPQGLR + Deamidated (NQ)
522 364.2136 726.4126 726.4137 -1.38 1 17 0.62 +1Score > 37 indicates identity
Score > 28 indicates homology
LDPARR
2322 523.2767 1566.8083 1566.7851 14.8 0 17 0.33 +1Score > 40 indicates identity
Score > 25 indicates homology
MSVNVASLDFLLIE + Deamidated (NQ); Oxidation (M)
2940 619.3267 1854.9583 1854.9323 14.0 0 17 0.61 +1Score > 39 indicates identity
Score > 27 indicates homology
DSSALQDPVLVNIQAQR + 2 Deamidated (NQ)
1128 496.2838 990.5530 990.5532 -0.13 1 17 0.85 +1Score > 40 indicates identity
Score > 29 indicates homology
ALVKNTMAK + Oxidation (M)
1850 669.8250 1337.6354 1337.6503 -11.1 1 17 0.82 +1Score > 41 indicates identity
Score > 29 indicates homology
NYVVLQPTEFE
1423 566.2832 1130.5518 1130.5642 -10.9 0 17 0.19 +1Score > 41 indicates identity
Score > 22 indicates homology
VVIQDPMGQK + Deamidated (NQ); Oxidation (M)
1391 560.8049 1119.5952 1119.5892 5.37 1 17 0.85 +1Score > 41 indicates identity
Score > 29 indicates homology
QMLMARLNK + Oxidation (M)
1543 589.2989 1176.5832 1176.5809 2.03 1 17 0.57 +1Score > 42 indicates identity
Score > 27 indicates homology
RVQLSTALCE + Deamidated (NQ)
2915 460.7475 1838.9609 1838.9924 -17.1 0 17 0.36 +1Score > 38 indicates identity
Score > 25 indicates homology
TSTIGLGMPTAPAAVLAPR + Oxidation (M)
950 460.2498 918.4850 918.4770 8.74 1 17 0.93 +1Score > 42 indicates identity
Score > 29 indicates homology
KNTNSNLK + Deamidated (NQ)
2841 601.3297 1800.9673 1800.9886 -11.9 1 17 0.85 +1Score > 38 indicates identity
Score > 29 indicates homology
IHSNFINDLLGGKFVK
2979 938.4531 1874.8916 1874.9131 -11.4 1 17 0.91 +1Score > 40 indicates identity
Score > 29 indicates homology
CAYHLMQQRIISNPK + Deamidated (NQ); Oxidation (M)
2365 793.4221 1584.8296 1584.8512 -13.6 0 17 0.88 +1Score > 40 indicates identity
Score > 29 indicates homology
TVILWDVAQGQVVR + 2 Deamidated (NQ)
1361 553.7922 1105.5698 1105.5655 3.90 0 17 0.49 +1Score > 42 indicates identity
Score > 26 indicates homology
FGSGINVLLGE + Deamidated (NQ)
2810 893.9837 1785.9528 1785.9261 15.0 0 17 0.84 +1Score > 39 indicates identity
Score > 29 indicates homology
LPGTYTALSPTPLNNAR + Deamidated (NQ)
463 348.1724 694.3302 694.3286 2.40 1 17 0.72 +1Score > 41 indicates identity
Score > 28 indicates homology
IDEYR
608 385.7300 769.4454 769.4446 1.07 0 17 0.65 +1Score > 37 indicates identity
Score > 28 indicates homology
LPNGTIR
2015 472.5766 1414.7080 1414.7027 3.72 1 17 0.75 +1Score > 41 indicates identity
Score > 28 indicates homology
AVETAVMPQWQR
2366 793.4329 1584.8512 1584.8512 0.052 0 17 0.66 +1Score > 39 indicates identity
Score > 28 indicates homology
TVILWDVAQGQVVR + 2 Deamidated (NQ)
733 414.7229 827.4312 827.4388 -9.18 1 17 0.68 +1Score > 38 indicates identity
Score > 28 indicates homology
IPNNEIK + Deamidated (NQ)
1781 648.3740 1294.7334 1294.7245 6.93 1 17 0.81 +1Score > 35 indicates identity
Score > 29 indicates homology
NTPSPLKNSPIK
1016 314.8367 941.4883 941.4858 2.62 0 17 0.49 +1Score > 40 indicates identity
Score > 26 indicates homology
QDYLLYK
2520 825.9324 1649.8502 1649.8624 -7.39 0 17 0.47 +1Score > 40 indicates identity
Score > 26 indicates homology
ITTVQAAIDFIQSSR + Deamidated (NQ)
861 440.7262 879.4378 879.4450 -8.14 1 17 0.75 +1Score > 42 indicates identity
Score > 28 indicates homology
TEPYRSK
750 418.2263 834.4380 834.4269 13.3 0 17 0.95 +1Score > 42 indicates identity
Score > 29 indicates homology
AAQTMGLK + Oxidation (M)
2573 557.3345 1668.9817 1668.9563 15.2 1 17 0.28 +1Score > 31 indicates identity
Score > 24 indicates homology
SRLPLLDLNIYVPR + Deamidated (NQ)
2749 879.9918 1757.9690 1757.9563 7.24 1 17 0.28 +1Score > 36 indicates identity
Score > 24 indicates homology
KLNSAGINILPQLTFE + Deamidated (NQ)
2892 912.9805 1823.9464 1823.9741 -15.2 0 17 0.47 +1Score > 39 indicates identity
Score > 26 indicates homology
NIPVSAGLAGGSADAAAIIR + Deamidated (NQ)
1218 345.5024 1033.4854 1033.4862 -0.84 0 17 0.96 +1Score > 41 indicates identity
Score > 29 indicates homology
GMADVAQIGR + Deamidated (NQ); Oxidation (M)
354 318.1927 634.3708 634.3690 2.91 0 17 0.94 +1Score > 39 indicates identity
Score > 29 indicates homology
ALGYLV
2174 749.9318 1497.8490 1497.8515 -1.63 1 17 0.74 +1Score > 36 indicates identity
Score > 28 indicates homology
TVLKRPVDLTNDK
1638 408.5609 1222.6609 1222.6531 6.38 1 17 0.45 +1Score > 39 indicates identity
Score > 26 indicates homology
IQVHRSGGDVR
2176 749.9348 1497.8550 1497.8402 9.90 1 17 0.92 +1Score > 36 indicates identity
Score > 29 indicates homology
VVNQLLSKAGNLLE + Deamidated (NQ)
942 457.7738 913.5330 913.5233 10.7 1 17 0.46 +1Score > 40 indicates identity
Score > 26 indicates homology
QNKTVVPK + Deamidated (NQ)
3426 715.0522 2142.1348 2142.1532 -8.59 0 17 0.39 +1Score > 37 indicates identity
Score > 25 indicates homology
IQQQLTTILSTAQQGQLLR + 3 Deamidated (NQ)
3367 706.0647 2115.1723 2115.1398 15.4 1 17 0.46 +1Score > 34 indicates identity
Score > 26 indicates homology
MPNTNPLFIDALASVVLKR + Deamidated (NQ); Oxidation (M)
3629 754.0661 2259.1765 2259.1575 8.38 1 17 0.29 +1Score > 38 indicates identity
Score > 24 indicates homology
NLLSFLRGTFLNNYVPFDK + 2 Deamidated (NQ)
1920 686.3441 1370.6736 1370.6830 -6.82 0 17 0.5 +1Score > 40 indicates identity
Score > 26 indicates homology
NIINLNSGHLFE + Deamidated (NQ)
1477 385.5653 1153.6741 1153.6819 -6.79 0 17 0.59 +1Score > 36 indicates identity
Score > 27 indicates homology
SVAIAVTLQPR
2162 747.8830 1493.7514 1493.7362 10.2 0 17 0.87 +1Score > 41 indicates identity
Score > 29 indicates homology
QVTLLGQNVNSYR + 3 Deamidated (NQ)
500 358.2035 714.3924 714.3912 1.76 0 17 0.61 +1Score > 42 indicates identity
Score > 27 indicates homology
TLPQQK + Deamidated (NQ)
679 400.7429 799.4712 799.4664 6.05 1 17 0.62 +1Score > 40 indicates identity
Score > 27 indicates homology
AVDARIR
710 408.7083 815.4020 815.4137 -14.3 0 17 0.85 +1Score > 42 indicates identity
Score > 28 indicates homology
LSPADGTR
1371 554.7997 1107.5848 1107.5998 -13.5 0 17 0.7 +1Score > 40 indicates identity
Score > 28 indicates homology
NFLMLLVDK + Oxidation (M)
3758 1170.0989 2338.1832 2338.1917 -3.61 1 17 0.22 +1Score > 38 indicates identity
Score > 23 indicates homology
LYTNVRPEASYNQSLSILDR
1583 399.8902 1196.6488 1196.6652 -13.7 0 17 0.91 +1Score > 39 indicates identity
Score > 29 indicates homology
IIQIQIDPQK + 2 Deamidated (NQ)
2163 747.8832 1493.7518 1493.7435 5.56 1 17 0.82 +1Score > 41 indicates identity
Score > 28 indicates homology
YIDNKITMAIPAE + Oxidation (M)
1712 421.5730 1261.6972 1261.6918 4.27 1 17 0.33 +1Score > 39 indicates identity
Score > 24 indicates homology
GLSVEFALLADK
2171 375.2012 1496.7757 1496.7691 4.43 1 17 0.81 +1Score > 39 indicates identity
Score > 28 indicates homology
AMTSGLLLASSMKR + 2 Oxidation (M)
1745 639.3710 1276.7274 1276.7292 -1.36 1 17 0.21 +1Score > 36 indicates identity
Score > 22 indicates homology
KALFAPFQVTR
2231 758.3654 1514.7162 1514.7293 -8.63 1 17 0.68 +1Score > 40 indicates identity
Score > 27 indicates homology
VFSVFALFENDVE
1912 684.3517 1366.6888 1366.6803 6.29 1 17 0.82 +1Score > 40 indicates identity
Score > 28 indicates homology
TSGFEMVQGLLGK + Deamidated (NQ)
2164 747.8834 1493.7522 1493.7362 10.8 0 17 0.88 +1Score > 41 indicates identity
Score > 29 indicates homology
QVTLLGQNVNSYR + 3 Deamidated (NQ)
640 392.7326 783.4506 783.4491 2.02 0 17 0.45 +1Score > 37 indicates identity
Score > 26 indicates homology
GDVPGLVK
1155 501.2961 1000.5776 1000.5917 -14.0 1 17 0.78 +1Score > 39 indicates identity
Score > 28 indicates homology
KQLLSQGVK + Deamidated (NQ)
1420 565.7614 1129.5082 1129.4928 13.7 1 17 0.2 +1Score > 39 indicates identity
Score > 22 indicates homology
IFTGTGNEYE
1475 576.8051 1151.5956 1151.6186 -19.9 1 17 0.72 +1Score > 40 indicates identity
Score > 28 indicates homology
KQGYSTGLIGK + Deamidated (NQ)
2481 545.9634 1634.8684 1634.8848 -10.0 1 17 0.97 +1Score > 39 indicates identity
Score > 29 indicates homology
VKMQVTMSLASLVGR + Oxidation (M)
2271 514.2898 1539.8476 1539.8297 11.6 1 17 0.7 +1Score > 38 indicates identity
Score > 27 indicates homology
LIGWVDQTPLLER + Deamidated (NQ)
2893 608.9906 1823.9500 1823.9529 -1.63 1 16 0.8 +1Score > 39 indicates identity
Score > 28 indicates homology
AIFAGANIIGINHRNLE + 2 Deamidated (NQ)
1355 551.2998 1100.5850 1100.5713 12.5 0 16 0.86 +1Score > 41 indicates identity
Score > 28 indicates homology
ILNNLSSDPK + Deamidated (NQ)
2511 550.9509 1649.8309 1649.8624 -19.1 0 16 0.24 +1Score > 40 indicates identity
Score > 23 indicates homology
ITTVQAAIDFIQSSR + Deamidated (NQ)
1854 671.3788 1340.7430 1340.7551 -9.00 0 16 0.3 +1Score > 39 indicates identity
Score > 24 indicates homology
LNPSLVISLSQGL + Deamidated (NQ)
2682 862.4720 1722.9294 1722.9266 1.66 1 16 0.19 +1Score > 38 indicates identity
Score > 22 indicates homology
YLPLLQGKSLAMIFE + Deamidated (NQ)
1620 608.3609 1214.7072 1214.7234 -13.3 1 16 0.53 +1Score > 36 indicates identity
Score > 26 indicates homology
LTKALTVQVDK
1864 450.5665 1348.6777 1348.6849 -5.37 1 16 0.57 +1Score > 41 indicates identity
Score > 26 indicates homology
WMSWESILLGK
1332 545.2818 1088.5490 1088.5349 13.0 1 16 0.83 +1Score > 42 indicates identity
Score > 28 indicates homology
QNITELQNK + 2 Deamidated (NQ)
3721 578.0319 2308.0985 2308.0933 2.24 1 16 0.095 +1Score > 39 indicates identity
Score > 19 indicates homology
FIILSDILGDEDHLGDMDFK + Oxidation (M)
585 380.2203 758.4260 758.4174 11.4 1 16 0.93 +1Score > 44 indicates identity
Score > 29 indicates homology
VGVDIEK
2402 536.2887 1605.8443 1605.8362 5.03 1 16 0.72 +1Score > 39 indicates identity
Score > 27 indicates homology
VQNYLQIDINTKR + 2 Deamidated (NQ)
3354 1056.0457 2110.0768 2110.0946 -8.42 1 16 0.67 +1Score > 39 indicates identity
Score > 27 indicates homology
AGQDIQEVIDNLPINVPFK + Deamidated (NQ)
1971 699.8881 1397.7616 1397.7377 17.1 0 16 0.83 +1Score > 39 indicates identity
Score > 28 indicates homology
VIAIVPYMAYSR + Oxidation (M)
1346 549.2781 1096.5416 1096.5474 -5.27 0 16 0.49 +1Score > 40 indicates identity
Score > 26 indicates homology
QTLALMFQK + 2 Deamidated (NQ); Oxidation (M)
4562 828.9130 3311.6229 3311.6354 -3.79 1 16 0.095 +1Score > 36 indicates identity
Score > 19 indicates homology
ARGAAAQGQTQTVAAQAQALAAQAAAAAHAAQAHR + 4 Deamidated (NQ)
2348 789.4216 1576.8286 1576.8283 0.23 1 16 0.15 +1Score > 40 indicates identity
Score > 20 indicates homology
LGILGVDMEAAGIYR
1913 684.3520 1366.6894 1366.6803 6.73 1 16 0.93 +1Score > 40 indicates identity
Score > 28 indicates homology
TSGFEMVQGLLGK + Deamidated (NQ)
4047 663.3160 2649.2349 2649.2116 8.79 1 16 0.31 +1Score > 37 indicates identity
Score > 24 indicates homology
GQTIKADQPLTIDPNVGGFGQMDTE + 2 Deamidated (NQ); Oxidation (M)
426 340.7039 679.3932 679.3904 4.13 1 16 0.48 +1Score > 35 indicates identity
Score > 26 indicates homology
KYQIK + Deamidated (NQ)
1905 456.2770 1365.8092 1365.8092 -0.023 1 16 0.63 +1Score > 32 indicates identity
Score > 27 indicates homology
TQLAGPLQRLLR + Deamidated (NQ)
1820 660.3819 1318.7492 1318.7497 -0.32 1 16 0.52 +1Score > 37 indicates identity
Score > 26 indicates homology
FLGTTKDPVTLK
2527 413.9629 1651.8225 1651.8352 -7.67 1 16 0.26 +1Score > 40 indicates identity
Score > 23 indicates homology
EIAAACGQLAAEPPVR
493 356.6752 711.3358 711.3340 2.58 1 16 0.36 +1Score > 39 indicates identity
Score > 24 indicates homology
NEFFR
1131 497.7708 993.5270 993.5165 10.7 1 16 0.79 +1Score > 40 indicates identity
Score > 28 indicates homology
GTISKNMVK + Deamidated (NQ); Oxidation (M)
678 400.7366 799.4586 799.4552 4.31 0 16 0.83 +1Score > 42 indicates identity
Score > 28 indicates homology
VLVGQGAR + Deamidated (NQ)
1899 683.8409 1365.6672 1365.6524 10.9 1 16 0.26 +1Score > 41 indicates identity
Score > 23 indicates homology
TANATALGYEQAR + Deamidated (NQ)
1065 479.2552 956.4958 956.4927 3.30 0 16 0.55 +1Score > 39 indicates identity
Score > 26 indicates homology
TITAHAQSK + Deamidated (NQ)
1225 518.2980 1034.5814 1034.5913 -9.53 0 16 0.42 +1Score > 38 indicates identity
Score > 25 indicates homology
LFGPLSFVR
1228 518.2994 1034.5842 1034.5760 7.97 1 16 0.12 +1Score > 38 indicates identity
Score > 20 indicates homology
IFAKNNISK + Deamidated (NQ)
1683 620.3246 1238.6346 1238.6394 -3.86 0 16 0.37 +1Score > 40 indicates identity
Score > 24 indicates homology
VTASLPTPVQPE + Deamidated (NQ)
2109 490.6092 1468.8058 1468.7998 4.07 0 16 0.43 +1Score > 38 indicates identity
Score > 25 indicates homology
AAAVGNIGVSVADAVR
2582 558.2963 1671.8671 1671.8832 -9.65 0 16 0.36 +1Score > 40 indicates identity
Score > 24 indicates homology
ADVVVTAVGKPNFVQE
2991 629.0074 1884.0004 1883.9993 0.58 0 16 0.45 +1Score > 38 indicates identity
Score > 25 indicates homology
LFNVVVTTAGALDHDIAK + Deamidated (NQ)
519 362.7220 723.4294 723.4279 2.12 0 16 0.79 +1Score > 34 indicates identity
Score > 28 indicates homology
DHVLLK
2388 799.4169 1596.8192 1596.7968 14.0 0 16 0.59 +1Score > 40 indicates identity
Score > 26 indicates homology
QGQQQQQLQQQQK
1541 588.8251 1175.6356 1175.6411 -4.64 1 16 0.51 +1Score > 40 indicates identity
Score > 26 indicates homology
AQDLTRFVAR
3467 720.3555 2158.0447 2158.0728 -13.0 0 16 0.43 +1Score > 39 indicates identity
Score > 25 indicates homology
LVFDAPAGPALNASLIDMGNR + Deamidated (NQ); Oxidation (M)
1330 544.7724 1087.5302 1087.5146 14.4 0 16 0.82 +1Score > 41 indicates identity
Score > 28 indicates homology
LTGQSDDQPK
1250 351.5180 1051.5322 1051.5298 2.27 1 16 0.9 +1Score > 41 indicates identity
Score > 28 indicates homology
QAASYIRDK + Deamidated (NQ)
1494 579.7747 1157.5348 1157.5499 -13.0 1 16 0.082 +1Score > 40 indicates identity
Score > 18 indicates homology
VKVHDMNNGK + Deamidated (NQ); Oxidation (M)
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