MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 501–600 (out of 3698)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2283 774.8882 1547.7618 1547.7831 -13.7 1 19 0.13 +1Score > 41 indicates identity
Score > 22 indicates homology		 NVVEVTFNDLAAQK + Deamidated (NQ)
2726 871.9930 1741.9714 1741.9475 13.7 0 18 0.33 +1Score > 36 indicates identity
Score > 26 indicates homology		 QLVQQGLGIFGGAIADR
3496 725.3649 2173.0729 2173.0611 5.43 1 18 0.3 +1Score > 39 indicates identity
Score > 26 indicates homology		 QNTRPVSEVNASLDLNTASR + 2 Deamidated (NQ)
1767 430.8857 1289.6353 1289.6099 19.7 0 18 0.29 +1Score > 42 indicates identity
Score > 26 indicates homology		 NGLLSNQLQSSE + Deamidated (NQ)
833 434.2046 866.3946 866.4021 -8.64 1 18 0.91 +1Score > 40 indicates identity
Score > 31 indicates homology		 VENYVNK + 2 Deamidated (NQ)
1220 345.8501 1034.5285 1034.5145 13.5 1 18 0.5 +1Score > 41 indicates identity
Score > 28 indicates homology		 GNEFGVVTGR
1253 527.7217 1053.4288 1053.4219 6.59 1 18 0.31 +1Score > 37 indicates identity
Score > 26 indicates homology		 SNGMLCSER + Deamidated (NQ)
1179 507.7924 1013.5702 1013.5869 -16.4 1 18 0.92 +1Score > 41 indicates identity
Score > 31 indicates homology		 KIDNLINGK
529 365.1988 728.3830 728.3817 1.87 0 18 0.95 +1Score > 39 indicates identity
Score > 31 indicates homology		 LAADPSR
345 315.1856 628.3566 628.3544 3.55 0 18 0.65 +1Score > 37 indicates identity
Score > 29 indicates homology		 AVNPTK
423 338.2064 674.3982 674.3963 2.91 0 18 0.99 +1Score > 40 indicates identity
Score > 31 indicates homology		 TAGVLSK
1879 452.5628 1354.6666 1354.6742 -5.62 1 18 0.18 +1Score > 40 indicates identity
Score > 23 indicates homology		 GAAVWGPRAQADR + Deamidated (NQ)
662 793.4011 792.3938 792.3800 17.5 1 18 0.39 +1Score > 42 indicates identity
Score > 27 indicates homology		 ASIAEMR + Oxidation (M)
888 446.7436 891.4726 891.4702 2.78 1 18 0.76 +1Score > 42 indicates identity
Score > 30 indicates homology		 VAEFIADK
1778 432.2748 1293.8026 1293.7768 19.9 1 18 0.68 +1Score > 29 indicates identity
Score > 29 indicates homology		 VVGNPIRNAILK + Deamidated (NQ)
464 348.7063 695.3980 695.3966 2.10 0 18 0.75 +1Score > 36 indicates identity
Score > 30 indicates homology		 APAAAPAK
2522 550.9617 1649.8633 1649.8737 -6.30 0 18 0.45 +1Score > 39 indicates identity
Score > 27 indicates homology		 DSGAPLQAPVGQAILGR + Deamidated (NQ)
724 825.5263 824.5190 824.5344 -18.7 1 18 0.44 +1Score > 31 indicates identity
Score > 27 indicates homology		 KVRPGIR
1609 607.3030 1212.5914 1212.5696 18.0 1 18 0.15 +1Score > 40 indicates identity
Score > 22 indicates homology		 MVTAQELDYK + Oxidation (M)
2877 907.4578 1812.9010 1812.9357 -19.1 1 18 0.13 +1Score > 40 indicates identity
Score > 22 indicates homology		 DDLLNLIDAPSVVAKTE + Deamidated (NQ)
881 444.7464 887.4782 887.4712 7.92 1 18 0.41 +1Score > 42 indicates identity
Score > 27 indicates homology		 SLNEVIGR + Deamidated (NQ)
276 585.3362 584.3289 584.3282 1.26 0 18 0.41 +1Score > 37 indicates identity
Score > 27 indicates homology		 QAIPR + Deamidated (NQ)
1542 589.2950 1176.5754 1176.5662 7.84 1 18 0.57 +1Score > 41 indicates identity
Score > 28 indicates homology		 AIAQAYDPEAK + Deamidated (NQ)
1604 604.8496 1207.6846 1207.6924 -6.46 1 18 0.31 +1Score > 36 indicates identity
Score > 26 indicates homology		 IIYNTLTRSK
1760 644.3268 1286.6390 1286.6579 -14.6 1 18 0.45 +1Score > 41 indicates identity
Score > 27 indicates homology		 ASNADDVLARVR + Deamidated (NQ)
3292 693.0538 2076.1396 2076.1004 18.9 1 18 1 +1Score > 35 indicates identity
Score > 31 indicates homology		 LNSAVFPGAQGGPLEHVIAAK + Deamidated (NQ)
1622 608.8138 1215.6130 1215.5983 12.1 1 18 0.8 +1Score > 41 indicates identity
Score > 30 indicates homology		 ESLATLNSVPGE
1592 600.3380 1198.6614 1198.6418 16.4 1 18 0.85 +1Score > 40 indicates identity
Score > 30 indicates homology		 DVLALARDGNR
2252 510.6223 1528.8451 1528.8249 13.2 0 18 0.62 +1Score > 38 indicates identity
Score > 29 indicates homology		 FNHQLTDLTSIIK
2381 797.3989 1592.7832 1592.7716 7.33 1 18 0.55 +1Score > 41 indicates identity
Score > 28 indicates homology		 LSMKAVATDVADNAAT + Oxidation (M)
1528 586.8320 1171.6494 1171.6448 3.95 1 18 0.65 +1Score > 40 indicates identity
Score > 29 indicates homology		 QLGLINEAVSK + Deamidated (NQ)
2431 538.3185 1611.9337 1611.9097 14.9 1 18 0.11 +1Score > 33 indicates identity
Score > 21 indicates homology		 QVAVNKLHILSFSR + Deamidated (NQ)
3110 986.5309 1971.0472 1971.0425 2.40 1 18 0.23 +1Score > 38 indicates identity
Score > 24 indicates homology		 ADVLSDTRALLSALDFHK
3398 710.0457 2127.1153 2127.0960 9.05 1 18 0.19 +1Score > 38 indicates identity
Score > 23 indicates homology		 AADTAGDLAGLEIAGPGFINVR
882 444.7578 887.5010 887.5076 -7.38 1 18 0.95 +1Score > 41 indicates identity
Score > 30 indicates homology		 KNASGLLGK + Deamidated (NQ)
943 457.7759 913.5372 913.5233 15.3 0 18 0.25 +1Score > 40 indicates identity
Score > 25 indicates homology		 GNLNVVAVK + Deamidated (NQ)
1629 611.3808 1220.7470 1220.7492 -1.79 0 18 0.1 +1Score > 29 indicates identity
Score > 21 indicates homology		 QLIPPLLTGLR + Deamidated (NQ)
2827 898.9930 1795.9714 1795.9540 9.70 1 18 0.31 +1Score > 37 indicates identity
Score > 25 indicates homology		 LNIASGSAVRLEPGQQR + Deamidated (NQ)
1990 703.3412 1404.6678 1404.6779 -7.17 1 18 0.18 +1Score > 41 indicates identity
Score > 23 indicates homology		 AAARMADDLASAAR + Oxidation (M)
2802 889.4603 1776.9060 1776.8716 19.4 0 18 0.17 +1Score > 40 indicates identity
Score > 23 indicates homology		 MVASNNITGATVFVPQE
2144 742.3710 1482.7274 1482.7255 1.29 1 18 0.26 +1Score > 40 indicates identity
Score > 25 indicates homology		 WNYLFGGNKQQK + Deamidated (NQ)
280 586.3845 585.3772 585.3737 5.94 0 18 0.69 +1Score > 39 indicates identity
Score > 29 indicates homology		 ILDIL
2823 598.3003 1791.8791 1791.8686 5.85 1 18 0.23 +1Score > 40 indicates identity
Score > 24 indicates homology		 MNWAISNLTGGNRVSR + Deamidated (NQ); Oxidation (M)
2862 904.9625 1807.9104 1807.9203 -5.47 0 18 0.42 +1Score > 40 indicates identity
Score > 27 indicates homology		 AQVDALLNSPNVDIPLE + Deamidated (NQ)
1278 356.2059 1065.5959 1065.5818 13.2 1 18 0.18 +1Score > 37 indicates identity
Score > 23 indicates homology		 LEDIPGIGPR
1412 564.7870 1127.5594 1127.5458 12.1 1 18 0.47 +1Score > 40 indicates identity
Score > 27 indicates homology		 QAAEALQPTAE
557 372.1884 742.3622 742.3610 1.73 1 18 0.87 +1Score > 38 indicates identity
Score > 30 indicates homology		 GPEDGLR
1798 655.3302 1308.6458 1308.6422 2.79 1 18 0.18 +1Score > 42 indicates identity
Score > 23 indicates homology		 KSANNAHQVPDK + Deamidated (NQ)
1673 619.7977 1237.5808 1237.5869 -4.86 1 18 0.42 +1Score > 41 indicates identity
Score > 27 indicates homology		 AMQICEMLVK + Oxidation (M)
1651 410.5762 1228.7068 1228.7027 3.34 1 18 0.68 +1Score > 38 indicates identity
Score > 29 indicates homology		 QLSEGLLALSAK
653 395.2068 788.3990 788.4140 -19.0 0 18 0.91 +1Score > 44 indicates identity
Score > 30 indicates homology		 SSQAGAIR
1601 602.8370 1203.6594 1203.6764 -14.1 1 18 0.64 +1Score > 40 indicates identity
Score > 28 indicates homology		 QGKFKPVSWK
2087 730.4050 1458.7954 1458.8194 -16.4 1 18 0.19 +1Score > 39 indicates identity
Score > 23 indicates homology		 LFEALQLAASQIR
2569 835.0170 1668.0194 1667.9974 13.2 1 18 0.16 +1Score > 25 indicates identity
Score > 22 indicates homology		 DPKIHGILVQLPLPK + Deamidated (NQ)
1518 585.3210 1168.6274 1168.6200 6.38 1 18 0.72 +1Score > 39 indicates identity
Score > 29 indicates homology		 LENAAAAASPVR
1700 627.3293 1252.6440 1252.6564 -9.89 0 18 0.48 +1Score > 40 indicates identity
Score > 27 indicates homology		 THFHLVDSIGK
3149 1000.0304 1998.0462 1998.0171 14.6 0 18 0.27 +1Score > 38 indicates identity
Score > 25 indicates homology		 LVIDAGNTNIVFAVHDGSR + Deamidated (NQ)
1552 1181.6923 1180.6850 1180.7067 -18.3 1 18 0.99 +1Score > 35 indicates identity
Score > 30 indicates homology		 LINLIEQIPK + Deamidated (NQ)
726 413.7564 825.4982 825.4960 2.72 0 18 0.99 +1Score > 36 indicates identity
Score > 30 indicates homology		 IPPVVSSK
405 336.2198 670.4250 670.4126 18.6 0 18 0.6 +1Score > 40 indicates identity
Score > 28 indicates homology		 KPSLAR
672 399.2309 796.4472 796.4555 -10.4 1 18 0.32 +1Score > 36 indicates identity
Score > 25 indicates homology		 KGHGISAK
805 427.7416 853.4686 853.4657 3.42 0 18 0.12 +1Score > 38 indicates identity
Score > 21 indicates homology		 APAAAPAASK
1495 1158.5989 1157.5916 1157.5928 -1.03 0 18 0.41 +1Score > 41 indicates identity
Score > 26 indicates homology		 GQDIGSLLGLGE
790 425.7255 849.4364 849.4344 2.35 1 18 0.14 +1Score > 42 indicates identity
Score > 22 indicates homology		 TANLEFR
279 586.3684 585.3611 585.3598 2.21 0 18 0.41 +1Score > 39 indicates identity
Score > 26 indicates homology		 AVAGIR
1466 383.8694 1148.5864 1148.6077 -18.6 1 18 0.99 +1Score > 42 indicates identity
Score > 30 indicates homology		 KQFIIDVASE
3905 621.5631 2482.2233 2482.2638 -16.3 0 18 0.31 +1Score > 38 indicates identity
Score > 25 indicates homology		 HCTFNLPDVQASKPSIAINLTR + Deamidated (NQ)
2870 604.3577 1810.0513 1810.0352 8.86 0 18 0.5 +1Score > 31 indicates identity
Score > 27 indicates homology		 QNQTIILALALQWAVK + Deamidated (NQ)
3010 948.0170 1894.0194 1893.9908 15.1 1 18 0.46 +1Score > 37 indicates identity
Score > 27 indicates homology		 INLLNQTIRHLASDQR + 3 Deamidated (NQ)
542 367.6849 733.3552 733.3653 -13.7 1 17 0.8 +1Score > 43 indicates identity
Score > 29 indicates homology		 TGRMNR
804 854.4753 853.4680 853.4657 2.69 0 17 0.87 +1Score > 38 indicates identity
Score > 29 indicates homology		 APAAAPAASK
889 894.4421 893.4348 893.4178 19.1 1 17 0.93 +1Score > 42 indicates identity
Score > 30 indicates homology		 FRMPDGR + Oxidation (M)
2987 627.6393 1879.8961 1879.8774 9.91 0 17 0.25 +1Score > 40 indicates identity
Score > 24 indicates homology		 AWQCGTVQLDYALPTR + 2 Deamidated (NQ)
1490 578.8528 1155.6910 1155.6863 4.11 1 17 0.92 +1Score > 36 indicates identity
Score > 30 indicates homology		 QLIDAAAKVVK + Deamidated (NQ)
2114 736.8712 1471.7278 1471.7493 -14.6 0 17 0.38 +1Score > 41 indicates identity
Score > 26 indicates homology		 GQVIEPLPNAMFR + Deamidated (NQ)
3281 519.2674 2073.0405 2073.0630 -10.8 0 17 0.11 +1Score > 39 indicates identity
Score > 20 indicates homology		 DITADALQQYQPDVLLIR + 2 Deamidated (NQ)
2826 599.3305 1794.9697 1794.9615 4.58 0 17 0.67 +1Score > 37 indicates identity
Score > 28 indicates homology		 DTLNALLAQGIVPIINE + 2 Deamidated (NQ)
2910 919.9548 1837.8950 1837.9172 -12.0 1 17 0.31 +1Score > 40 indicates identity
Score > 25 indicates homology		 VYSMFKSVILATFTSE + Oxidation (M)
982 465.2763 928.5380 928.5341 4.20 1 17 0.79 +1Score > 41 indicates identity
Score > 29 indicates homology		 AILNETLR
1630 611.3815 1220.7484 1220.7492 -0.65 0 17 0.082 +1Score > 29 indicates identity
Score > 19 indicates homology		 QLIPPLLTGLR + Deamidated (NQ)
337 314.1957 626.3768 626.3864 -15.2 1 17 0.38 +1Score > 37 indicates identity
Score > 26 indicates homology		 APGVKR
2539 829.9375 1657.8604 1657.8675 -4.28 0 17 0.29 +1Score > 40 indicates identity
Score > 25 indicates homology		 VQSQNGIVLSWSVIE
664 397.2092 792.4038 792.4017 2.65 1 17 0.83 +1Score > 42 indicates identity
Score > 29 indicates homology		 FQEAGIK + Deamidated (NQ)
759 419.7445 837.4744 837.4708 4.33 1 17 0.85 +1Score > 37 indicates identity
Score > 29 indicates homology		 AAIHGIEK
1914 684.3613 1366.7080 1366.7092 -0.85 1 17 0.54 +1Score > 40 indicates identity
Score > 27 indicates homology		 LASKTDINYNTK
3004 947.4995 1892.9844 1892.9731 6.00 1 17 0.66 +1Score > 39 indicates identity
Score > 28 indicates homology		 NFNVSVNILQGKISQTK + 4 Deamidated (NQ)
713 409.2200 816.4254 816.4202 6.43 1 17 0.77 +1Score > 43 indicates identity
Score > 29 indicates homology		 NSQRGVR + Deamidated (NQ)
1001 313.8354 938.4844 938.4821 2.39 0 17 0.94 +1Score > 39 indicates identity
Score > 30 indicates homology		 GPLNPDGLR + Deamidated (NQ)
2083 485.9560 1454.8462 1454.8318 9.91 1 17 0.5 +1Score > 34 indicates identity
Score > 27 indicates homology		 TAVVVGAGRSGLAAAR
296 601.3311 600.3238 600.3343 -17.5 0 17 0.83 +1Score > 42 indicates identity
Score > 29 indicates homology		 GAANLR
598 382.2271 762.4396 762.4527 -17.1 0 17 0.98 +1Score > 40 indicates identity
Score > 30 indicates homology		 IIYLLE
1910 456.5701 1366.6885 1366.7133 -18.1 0 17 0.66 +1Score > 40 indicates identity
Score > 28 indicates homology		 IYAVGDIATYPGK
2382 532.2674 1593.7804 1593.8072 -16.8 0 17 0.9 +1Score > 41 indicates identity
Score > 29 indicates homology		 NAALAMFNVIIDTGK + Deamidated (NQ); Oxidation (M)
2513 825.9270 1649.8394 1649.8624 -13.9 0 17 0.59 +1Score > 40 indicates identity
Score > 27 indicates homology		 ITTVQAAIDFIQSSR + Deamidated (NQ)
637 390.2277 778.4408 778.4378 3.97 0 17 0.74 +1Score > 37 indicates identity
Score > 28 indicates homology		 IPFGFAK
1774 431.9079 1292.7019 1292.7128 -8.49 1 17 0.21 +1Score > 39 indicates identity
Score > 23 indicates homology		 QLPFDKYLLR + Deamidated (NQ)
2298 519.2970 1554.8692 1554.8617 4.80 0 17 0.25 +1Score > 37 indicates identity
Score > 24 indicates homology		 VTQLLSLLANVQQE
1952 693.9056 1385.7966 1385.8064 -7.07 0 17 0.48 +1Score > 36 indicates identity
Score > 27 indicates homology		 VSGIMVAAILAGLR + Oxidation (M)
1058 319.5149 955.5229 955.5273 -4.65 0 17 0.94 +1Score > 39 indicates identity
Score > 29 indicates homology		 AACALPVVR
1923 458.2264 1371.6574 1371.6816 -17.7 1 17 0.72 +1Score > 40 indicates identity
Score > 28 indicates homology		 SRMGLSVLLDHE + Oxidation (M)
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