MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 401–500 (out of 3698)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2350 790.4476 1578.8806 1578.8729 4.89 1 20 0.4 +1Score > 36 indicates identity
Score > 29 indicates homology		 ELQANVVHLSLLSR + Deamidated (NQ)
3435 716.0629 2145.1669 2145.1331 15.8 0 20 0.79 +1Score > 35 indicates identity
Score > 32 indicates homology		 INLALHVTGQRPDGYHLLE
2046 715.8342 1429.6538 1429.6797 -18.1 1 20 0.2 +1Score > 39 indicates identity
Score > 26 indicates homology		 QALNGNDNVQRAK + 3 Deamidated (NQ)
1231 519.2873 1036.5600 1036.5553 4.60 0 20 0.13 +1Score > 39 indicates identity
Score > 24 indicates homology		 LSVNNISYK
1572 595.8287 1189.6428 1189.6554 -10.5 1 20 0.2 +1Score > 40 indicates identity
Score > 26 indicates homology		 GITLASASTLEK
275 583.3983 582.3910 582.3853 9.78 0 20 0.2 +1Score > 26 indicates identity VPVIR
3379 530.2791 2117.0873 2117.0905 -1.54 0 20 0.072 +1Score > 39 indicates identity
Score > 21 indicates homology		 QYIAQHFVAISTNANDVVK
1568 594.8212 1187.6278 1187.6397 -10.0 1 20 0.88 +1Score > 41 indicates identity
Score > 32 indicates homology		 LAAQVDLSKNK + 2 Deamidated (NQ)
2668 572.3165 1713.9277 1713.9148 7.49 0 20 0.41 +1Score > 38 indicates identity
Score > 29 indicates homology		 ISLLLTDDAAVQALNR + 2 Deamidated (NQ)
1571 397.5548 1189.6426 1189.6190 19.8 1 20 0.92 +1Score > 40 indicates identity
Score > 32 indicates homology		 AAIKDSDTIQK + Deamidated (NQ)
316 307.7089 613.4032 613.3911 19.7 0 20 0.97 +1Score > 40 indicates identity
Score > 33 indicates homology		 AVVGLR
1785 434.2072 1299.5998 1299.6208 -16.1 1 20 0.27 +1Score > 40 indicates identity
Score > 27 indicates homology		 QRHDTDTIWK + Deamidated (NQ)
237 529.3471 528.3398 528.3384 2.76 0 20 0.87 +1Score > 41 indicates identity
Score > 32 indicates homology		 AVIAR
1002 470.3005 938.5864 938.5800 6.82 0 20 0.85 +1Score > 32 indicates identity
Score > 32 indicates homology		 LLAVAPQVK + Deamidated (NQ)
1339 547.7845 1093.5544 1093.5516 2.60 0 20 0.94 +1Score > 41 indicates identity
Score > 32 indicates homology		 AATNGAPGVAHK + Deamidated (NQ)
2349 789.4244 1576.8342 1576.8395 -3.34 1 20 0.25 +1Score > 39 indicates identity
Score > 27 indicates homology		 FLGNLMQLLNNKR + Deamidated (NQ); Oxidation (M)
1120 492.3311 982.6476 982.6427 5.08 1 20 0.6 +1Score > 30 indicates identity VLVAVELLK
1896 683.3221 1364.6296 1364.6077 16.1 1 20 0.2 +1Score > 40 indicates identity
Score > 26 indicates homology		 NRLNMNNWMR + Deamidated (NQ); Oxidation (M)
1984 468.9139 1403.7199 1403.6946 18.0 0 20 0.16 +1Score > 41 indicates identity
Score > 25 indicates homology		 NFHQHQITHLK + 2 Deamidated (NQ)
1455 571.8614 1141.7082 1141.7070 1.05 0 20 0.69 +1Score > 31 indicates identity ISIQVLQTLK
300 602.3524 601.3451 601.3435 2.69 0 20 0.84 +1Score > 45 indicates identity
Score > 32 indicates homology		 LSAQIA
1336 547.3093 1092.6040 1092.6040 0.069 1 20 0.86 +1Score > 38 indicates identity
Score > 32 indicates homology		 AAIQRGHLVE
1468 575.7801 1149.5456 1149.5336 10.5 1 20 0.59 +1Score > 41 indicates identity
Score > 30 indicates homology		 SMDEISAQAAK
2406 804.8942 1607.7738 1607.7732 0.38 1 20 0.27 +1Score > 41 indicates identity
Score > 27 indicates homology		 LGWAYQGPEQGFVR + Deamidated (NQ)
597 382.2098 762.4050 762.4024 3.45 0 20 0.95 +1Score > 43 indicates identity
Score > 32 indicates homology		 FANGLNK
884 891.5003 890.4930 890.5081 -17.0 1 20 0.42 +1Score > 40 indicates identity
Score > 29 indicates homology		 KMALLGMK
1633 611.8096 1221.6046 1221.6023 1.91 1 20 0.51 +1Score > 42 indicates identity
Score > 29 indicates homology		 QKGLSSAMLDR + Deamidated (NQ); Oxidation (M)
1959 463.9209 1388.7409 1388.7333 5.45 1 20 0.25 +1Score > 40 indicates identity
Score > 26 indicates homology		 MLEISQQLSLAR + Deamidated (NQ)
461 347.7042 693.3938 693.3810 18.6 0 20 0.17 +1Score > 39 indicates identity
Score > 25 indicates homology		 AVSVYR
722 412.7543 823.4940 823.5028 -10.6 0 20 0.59 +1Score > 34 indicates identity
Score > 30 indicates homology		 VRPSLPR
1888 682.3266 1362.6386 1362.6627 -17.6 1 20 0.78 +1Score > 41 indicates identity
Score > 31 indicates homology		 SSKADLQGSASPSK + Deamidated (NQ)
1964 695.8277 1389.6408 1389.6273 9.75 1 20 0.33 +1Score > 40 indicates identity
Score > 28 indicates homology		 ESTPGGAPGYQGNR
1191 510.2806 1018.5466 1018.5335 12.9 0 20 0.17 +1Score > 41 indicates identity
Score > 25 indicates homology		 AGFNLLLAAE + Deamidated (NQ)
1647 410.2278 1227.6616 1227.6571 3.64 1 20 0.71 +1Score > 40 indicates identity
Score > 31 indicates homology		 QNGRLLQQLR + 3 Deamidated (NQ)
840 435.2595 868.5044 868.5018 3.05 1 20 0.69 +1Score > 37 indicates identity
Score > 31 indicates homology		 EGINPLVK
1073 480.7735 959.5324 959.5287 3.87 1 20 0.77 +1Score > 41 indicates identity
Score > 31 indicates homology		 LIKTGDNAK + Deamidated (NQ)
3179 1006.5244 2011.0342 2011.0473 -6.51 1 20 0.21 +1Score > 39 indicates identity
Score > 26 indicates homology		 GQIVDTVVQEQRPSVLLE + 2 Deamidated (NQ)
1800 437.2230 1308.6472 1308.6561 -6.84 1 20 0.27 +1Score > 41 indicates identity
Score > 27 indicates homology		 QNITETSFVLR + 2 Deamidated (NQ)
3108 658.0025 1970.9857 1970.9659 10.0 0 20 0.9 +1Score > 40 indicates identity
Score > 32 indicates homology		 IGQMAQQPAAFIPDAALVE + 2 Deamidated (NQ)
894 447.7389 893.4632 893.4607 2.89 1 20 0.74 +1Score > 41 indicates identity
Score > 31 indicates homology		 LSGELYGR
1593 600.3391 1198.6636 1198.6418 18.2 1 20 0.7 +1Score > 40 indicates identity
Score > 31 indicates homology		 DVLALARDGNR
425 340.6805 679.3464 679.3442 3.32 0 20 0.77 +1Score > 40 indicates identity
Score > 31 indicates homology		 FPNFR
777 423.2414 844.4682 844.4654 3.36 0 20 0.81 +1Score > 43 indicates identity
Score > 31 indicates homology		 LSGINVNK + Deamidated (NQ)
745 417.2203 832.4260 832.4331 -8.44 0 20 0.48 +1Score > 43 indicates identity
Score > 29 indicates homology		 VDPQVFK + Deamidated (NQ)
2579 836.4310 1670.8474 1670.8475 -0.042 1 20 0.19 +1Score > 40 indicates identity
Score > 25 indicates homology		 AVQTLDDLLNAGVEGR + Deamidated (NQ)
1040 316.8561 947.5465 947.5328 14.5 1 20 0.23 +1Score > 38 indicates identity
Score > 26 indicates homology		 YSLPEVIK
2572 835.4965 1668.9784 1668.9661 7.37 1 20 0.62 +1Score > 31 indicates identity
Score > 30 indicates homology		 LNIILQGNIILSTEK + Deamidated (NQ)
3417 712.7344 2135.1814 2135.2201 -18.2 1 20 0.49 +1Score > 34 indicates identity
Score > 29 indicates homology		 DLALVTRQLATLVQAALPIE + Deamidated (NQ)
3080 488.7493 1950.9681 1950.9660 1.07 1 20 0.17 +1Score > 40 indicates identity
Score > 24 indicates homology		 SNRHIYAQVIDDVHQR + Deamidated (NQ)
550 368.7280 735.4414 735.4391 3.13 0 20 0.17 +1Score > 34 indicates identity
Score > 24 indicates homology		 PLTHIR
2169 748.9106 1495.8066 1495.8147 -5.38 0 19 0.77 +1Score > 38 indicates identity
Score > 31 indicates homology		 WIGEPQTILLNGR
1462 573.8173 1145.6200 1145.6180 1.83 0 19 0.27 +1Score > 40 indicates identity
Score > 26 indicates homology		 LTALAVSQLTE + Deamidated (NQ)
551 369.1959 736.3772 736.3755 2.35 1 19 0.73 +1Score > 42 indicates identity
Score > 31 indicates homology		 ENLAYK
1842 444.5918 1330.7536 1330.7344 14.4 1 19 0.15 +1Score > 37 indicates identity
Score > 24 indicates homology		 TTVQIKGDLLDK + Deamidated (NQ)
2130 492.8982 1475.6728 1475.6932 -13.9 1 19 0.14 +1Score > 40 indicates identity
Score > 23 indicates homology		 IAEGYSVFANGGYK + Deamidated (NQ)
1688 414.2372 1239.6898 1239.6823 6.03 1 19 0.5 +1Score > 38 indicates identity
Score > 29 indicates homology		 RGADILLVGTPE
895 447.7389 893.4632 893.4607 2.89 1 19 0.89 +1Score > 41 indicates identity
Score > 31 indicates homology		 LSGELYGR
2424 538.2911 1611.8515 1611.8580 -4.05 1 19 0.53 +1Score > 39 indicates identity
Score > 29 indicates homology		 LSARPGNEVNLLDSK
3471 721.0480 2160.1222 2160.1063 7.37 0 19 0.69 +1Score > 38 indicates identity
Score > 30 indicates homology		 TDPYVTPGSVINNLSINTVR + Deamidated (NQ)
655 395.2360 788.4574 788.4432 18.0 0 19 0.24 +1Score > 39 indicates identity
Score > 26 indicates homology		 LFGPDLK
2517 825.9296 1649.8446 1649.8157 17.6 1 19 0.76 +1Score > 40 indicates identity
Score > 31 indicates homology		 ILMVAWMNAEALQK + Deamidated (NQ); 2 Oxidation (M)
779 424.6996 847.3846 847.3937 -10.6 1 19 0.28 +1Score > 41 indicates identity
Score > 26 indicates homology		 DDTWRR
1965 695.8746 1389.7346 1389.7616 -19.4 0 19 0.77 +1Score > 41 indicates identity
Score > 31 indicates homology		 LQYAGLLSQLQR + Deamidated (NQ)
2043 714.8936 1427.7726 1427.7806 -5.57 0 19 0.64 +1Score > 40 indicates identity
Score > 30 indicates homology		 AAAQGLMAGINAVLK + Deamidated (NQ)
2405 804.4260 1606.8374 1606.8467 -5.76 1 19 0.5 +1Score > 40 indicates identity
Score > 29 indicates homology		 VNYKIANTGNSLWK
2813 596.6558 1786.9456 1786.9101 19.9 0 19 0.49 +1Score > 39 indicates identity
Score > 29 indicates homology		 LPGTYTALSPTPLNNAR + 2 Deamidated (NQ)
880 888.4854 887.4781 887.4713 7.74 0 19 0.99 +1Score > 42 indicates identity
Score > 32 indicates homology		 TVDGQVLR + Deamidated (NQ)
2318 783.9034 1565.7922 1565.8236 -20.0 1 19 0.24 +1Score > 41 indicates identity
Score > 25 indicates homology		 TAPKTTALSVAGMFR + Oxidation (M)
435 343.2101 684.4056 684.4170 -16.6 0 19 0.42 +1Score > 35 indicates identity
Score > 28 indicates homology		 GVALTPK
220 503.3190 502.3117 502.3115 0.46 0 19 0.96 +1Score > 44 indicates identity
Score > 31 indicates homology		 ASVVK
434 343.2048 684.3950 684.4031 -11.8 1 19 0.28 +1Score > 36 indicates identity
Score > 26 indicates homology		 AVSPRR
957 460.7469 919.4792 919.4797 -0.50 0 19 0.46 +1Score > 42 indicates identity
Score > 28 indicates homology		 LSCQVSVK
2113 736.8670 1471.7194 1471.7381 -12.7 0 19 0.35 +1Score > 40 indicates identity
Score > 27 indicates homology		 WALMIDPQGQALK + 2 Deamidated (NQ)
2828 599.6656 1795.9750 1795.9832 -4.60 1 19 0.36 +1Score > 37 indicates identity
Score > 27 indicates homology		 TDGVVLKLNDVALWPR + Deamidated (NQ)
2346 788.9165 1575.8184 1575.8079 6.72 1 19 0.45 +1Score > 40 indicates identity
Score > 28 indicates homology		 NMLLYPSPRLNNK + Deamidated (NQ); Oxidation (M)
1831 663.4136 1324.8126 1324.8078 3.65 1 19 0.15 +1Score > 30 indicates identity
Score > 23 indicates homology		 NVVVVAANGKILK + Deamidated (NQ)
1884 453.5820 1357.7242 1357.7024 16.1 0 19 0.3 +1Score > 40 indicates identity
Score > 26 indicates homology		 QEPMLAGVIAGTR + Oxidation (M)
3397 710.0456 2127.1150 2127.0749 18.8 1 19 0.64 +1Score > 38 indicates identity
Score > 29 indicates homology		 IHSTFTIQVEYGTGPHAIR + Deamidated (NQ)
3315 1044.5276 2087.0406 2087.0357 2.36 0 19 0.7 +1Score > 39 indicates identity
Score > 30 indicates homology		 INITANNMPVGLGEPIFDR + Deamidated (NQ); Oxidation (M)
282 586.3851 585.3778 585.3737 6.96 0 19 0.48 +1Score > 39 indicates identity
Score > 28 indicates homology		 ILDIL
596 382.2096 762.4046 762.4058 -1.52 0 19 0.56 +1Score > 43 indicates identity
Score > 29 indicates homology		 MIQTVR + Oxidation (M)
1156 501.7493 1001.4840 1001.5029 -18.8 0 19 0.23 +1Score > 42 indicates identity
Score > 25 indicates homology		 VANLGNLGDK + 2 Deamidated (NQ)
1470 575.8069 1149.5992 1149.6030 -3.22 0 19 0.98 +1Score > 41 indicates identity
Score > 31 indicates homology		 NPNIVNVHIK + 3 Deamidated (NQ)
1619 304.6740 1214.6669 1214.6619 4.14 1 19 0.4 +1Score > 40 indicates identity
Score > 27 indicates homology		 VASLNKQIGQR + 2 Deamidated (NQ)
3230 510.5147 2038.0297 2038.0232 3.19 1 19 0.074 +1Score > 39 indicates identity
Score > 20 indicates homology		 IFILHADHEQNASTSTVR
512 361.2170 720.4194 720.4283 -12.2 0 19 0.59 +1Score > 38 indicates identity
Score > 29 indicates homology		 IGHAVPK
1230 519.2740 1036.5334 1036.5488 -14.8 1 19 0.28 +1Score > 40 indicates identity
Score > 26 indicates homology		 LAAAFKMNR + Oxidation (M)
491 354.6884 707.3622 707.3602 2.85 0 19 0.6 +1Score > 43 indicates identity
Score > 29 indicates homology		 FIADSR
1610 405.2047 1212.5923 1212.5696 18.7 0 19 0.35 +1Score > 40 indicates identity
Score > 27 indicates homology		 MLYQQIASNK + 2 Deamidated (NQ); Oxidation (M)
2256 766.9337 1531.8528 1531.8722 -12.6 0 19 0.19 +1Score > 37 indicates identity
Score > 24 indicates homology		 INLGHPLVAALADTK
3175 1006.4918 2010.9690 2010.9660 1.51 0 19 0.23 +1Score > 40 indicates identity
Score > 25 indicates homology		 QLTHQQIYPHFADGSNR
1175 337.8669 1010.5789 1010.5621 16.6 0 19 0.78 +1Score > 36 indicates identity
Score > 30 indicates homology		 AGDLQVRPR
2557 834.9461 1667.8776 1667.8981 -12.3 1 19 0.32 +1Score > 39 indicates identity
Score > 26 indicates homology		 ANLNTVLDVLEPLKE + Deamidated (NQ)
2209 502.6058 1504.7956 1504.7773 12.1 1 19 0.86 +1Score > 40 indicates identity
Score > 31 indicates homology		 LYDLKAQGLDLQE
1107 488.7600 975.5054 975.4947 11.1 1 19 0.86 +1Score > 42 indicates identity
Score > 31 indicates homology		 LEMDDLLK
1280 534.2745 1066.5344 1066.5481 -12.8 0 19 0.94 +1Score > 40 indicates identity
Score > 31 indicates homology		 IVFSMSNIR + Deamidated (NQ)
1083 482.7522 963.4898 963.4947 -4.99 1 19 0.83 +1Score > 42 indicates identity
Score > 30 indicates homology		 ELMVLTNK + Deamidated (NQ); Oxidation (M)
1723 635.3870 1268.7594 1268.7751 -12.3 1 19 0.9 +1Score > 34 indicates identity
Score > 31 indicates homology		 LLVAMAAVVARR
739 415.6942 829.3738 829.3817 -9.52 1 19 0.66 +1Score > 37 indicates identity
Score > 29 indicates homology		 NQDPEVK + Deamidated (NQ)
470 351.2038 700.3930 700.3868 8.95 1 19 0.77 +1Score > 42 indicates identity
Score > 30 indicates homology		 IGGAVER
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