MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 301–400 (out of 3698)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
391 333.1855 664.3564 664.3544 3.06 1 23 0.074 +1Score > 41 indicates identity
Score > 24 indicates homology		 VEVYR
993 467.7550 933.4954 933.4920 3.72 1 23 0.27 +1Score > 41 indicates identity
Score > 29 indicates homology		 TPVEAVYR
1176 337.8706 1010.5900 1010.5873 2.66 0 23 0.22 +1Score > 36 indicates identity
Score > 29 indicates homology		 VGATPIGLGAR
611 386.7142 771.4138 771.4239 -13.0 0 23 0.14 +1Score > 41 indicates identity
Score > 26 indicates homology		 NVVDLGR
1775 432.2297 1293.6673 1293.6677 -0.34 0 23 0.12 +1Score > 41 indicates identity
Score > 26 indicates homology		 ADHQGGLDGILAK
1882 678.8549 1355.6952 1355.6820 9.77 1 23 0.84 +1Score > 40 indicates identity
Score > 34 indicates homology		 AAVIVPQTLDEAE + Deamidated (NQ)
711 408.7467 815.4788 815.4865 -9.40 0 22 0.95 +1Score > 42 indicates identity
Score > 35 indicates homology		 VTLQTVR
1722 635.3802 1268.7458 1268.7704 -19.3 0 22 0.94 +1Score > 35 indicates identity
Score > 35 indicates homology		 LIVIDLLSVQR + Deamidated (NQ)
1057 478.2878 954.5610 954.5750 -14.6 1 22 0.63 +1Score > 36 indicates identity
Score > 33 indicates homology		 ILKDIQPK + Deamidated (NQ)
1362 553.7981 1105.5816 1105.5689 11.5 1 22 0.32 +1Score > 41 indicates identity
Score > 30 indicates homology		 AMLIVADKTE + Oxidation (M)
483 352.2225 702.4304 702.4276 4.08 0 22 0.43 +1Score > 38 indicates identity
Score > 31 indicates homology		 VAATTIK
977 464.7126 927.4106 927.3974 14.3 1 22 0.16 +1Score > 38 indicates identity
Score > 27 indicates homology		 EFFDQNK + Deamidated (NQ)
1392 560.8060 1119.5974 1119.5924 4.52 1 22 0.55 +1Score > 41 indicates identity
Score > 32 indicates homology		 VAQLYLEQR + Deamidated (NQ)
1509 387.8897 1160.6473 1160.6587 -9.85 0 22 0.17 +1Score > 39 indicates identity
Score > 27 indicates homology		 MLLITANTLR + Oxidation (M)
1859 672.8509 1343.6872 1343.6681 14.2 0 22 0.48 +1Score > 41 indicates identity
Score > 32 indicates homology		 QDVSLALDVDNR
1020 471.7731 941.5316 941.5182 14.3 0 22 0.98 +1Score > 39 indicates identity
Score > 35 indicates homology		 LTIPDNLR + Deamidated (NQ)
917 451.2555 900.4964 900.4916 5.36 0 22 0.58 +1Score > 42 indicates identity
Score > 32 indicates homology		 DINVAALGK + Deamidated (NQ)
1697 417.9066 1250.6980 1250.7095 -9.22 1 22 0.15 +1Score > 37 indicates identity
Score > 26 indicates homology		 KAGADGITIHLR
1839 665.3462 1328.6778 1328.6936 -11.8 1 22 0.44 +1Score > 41 indicates identity
Score > 31 indicates homology		 QVVVNLLGSRGGE + 2 Deamidated (NQ)
2282 774.3989 1546.7832 1546.8103 -17.5 1 22 0.53 +1Score > 41 indicates identity
Score > 32 indicates homology		 EAAAPGAAPAAAAAAPAAK
1344 365.9021 1094.6845 1094.7063 -19.9 1 22 0.26 +1Score > 30 indicates identity
Score > 29 indicates homology		 AIVLPKNLVK + Deamidated (NQ)
2307 520.9501 1559.8285 1559.8096 12.1 1 22 0.27 +1Score > 40 indicates identity
Score > 29 indicates homology		 IFEQLNGAWDVLR
1570 595.3398 1188.6650 1188.6602 4.09 0 22 0.86 +1Score > 39 indicates identity
Score > 34 indicates homology		 TAVLVTAVSVTE
1512 581.8094 1161.6042 1161.5877 14.2 0 22 0.9 +1Score > 42 indicates identity
Score > 34 indicates homology		 TSGTVDVQNLK + Deamidated (NQ)
1249 524.7772 1047.5398 1047.5237 15.4 0 22 0.82 +1Score > 42 indicates identity
Score > 34 indicates homology		 IGSGFDLPDK
1201 514.2891 1026.5636 1026.5651 -1.41 0 22 0.14 +1Score > 39 indicates identity
Score > 26 indicates homology		 LGFGFLFAR
1146 333.1951 996.5635 996.5465 17.1 1 22 0.43 +1Score > 37 indicates identity
Score > 31 indicates homology		 QARNGVPVR + Deamidated (NQ)
1589 599.3412 1196.6678 1196.6877 -16.6 0 22 0.63 +1Score > 38 indicates identity
Score > 32 indicates homology		 NLSAPVTLNLR
979 310.5132 928.5178 928.5342 -17.7 1 22 0.76 +1Score > 41 indicates identity
Score > 33 indicates homology		 KVVTPQTR + Deamidated (NQ)
641 392.7331 783.4516 783.4490 3.35 1 22 0.91 +1Score > 37 indicates identity
Score > 34 indicates homology		 KNPLPSK + Deamidated (NQ)
3338 1051.5105 2101.0064 2101.0188 -5.89 1 22 0.071 +1Score > 39 indicates identity
Score > 23 indicates homology		 GSRSVDHGYLQQIAQVADR + 2 Deamidated (NQ)
819 429.7629 857.5112 857.5083 3.47 1 22 0.62 +1Score > 41 indicates identity
Score > 32 indicates homology		 NASGLLKR
2568 835.0080 1668.0014 1667.9974 2.42 1 22 0.17 +1Score > 28 indicates identity
Score > 27 indicates homology		 DPKIHGILVQLPLPK + Deamidated (NQ)
1566 594.3203 1186.6260 1186.6346 -7.19 1 22 0.74 +1Score > 41 indicates identity
Score > 33 indicates homology		 AKASLIAQAWE
2071 722.4244 1442.8342 1442.8133 14.5 1 22 0.93 +1Score > 35 indicates identity
Score > 34 indicates homology		 EAIIVTLPPATYR
2658 856.9705 1711.9264 1711.9581 -18.5 1 22 0.2 +1Score > 38 indicates identity
Score > 27 indicates homology		 DNITVTVLGVKGNQVR
242 531.3263 530.3190 530.3177 2.58 0 22 0.66 +1Score > 46 indicates identity
Score > 32 indicates homology		 VVTGR
1119 328.5351 982.5835 982.5672 16.6 1 22 0.24 +1Score > 36 indicates identity
Score > 28 indicates homology		 AGGRPQLKR + Deamidated (NQ)
2895 609.2986 1824.8740 1824.8968 -12.5 0 22 0.52 +1Score > 40 indicates identity
Score > 31 indicates homology		 DVVEPYLIQQGFLMR + 2 Deamidated (NQ); Oxidation (M)
1165 503.2589 1004.5032 1004.5139 -10.6 0 22 0.59 +1Score > 42 indicates identity
Score > 32 indicates homology		 TDVVTDLSR
549 736.4011 735.3938 735.3915 3.13 0 22 0.87 +1Score > 41 indicates identity
Score > 34 indicates homology		 DILGYR
607 385.2511 768.4876 768.4858 2.45 0 22 0.92 +1Score > 34 indicates identity TIGIPLR
2577 557.3368 1668.9886 1668.9563 19.4 1 22 0.11 +1Score > 30 indicates identity
Score > 24 indicates homology		 SRLPLLDLNIYVPR + Deamidated (NQ)
3249 512.7687 2047.0457 2047.0078 18.5 1 22 0.72 +1Score > 39 indicates identity
Score > 33 indicates homology		 IKQAMVIAGADQSCDALVR + 2 Deamidated (NQ)
2785 884.9376 1767.8606 1767.8468 7.85 1 22 0.23 +1Score > 40 indicates identity
Score > 28 indicates homology		 TENAQLYPVGIYGWR + 2 Deamidated (NQ)
446 344.7050 687.3954 687.4027 -10.6 1 22 0.51 +1Score > 42 indicates identity
Score > 31 indicates homology		 AIKSNR
2257 766.9372 1531.8598 1531.8320 18.2 1 22 0.23 +1Score > 36 indicates identity
Score > 28 indicates homology		 AGDVIPYVVMVIKE
1671 413.2118 1236.6136 1236.6350 -17.3 1 22 0.64 +1Score > 41 indicates identity
Score > 32 indicates homology		 KTFTGNVAITGE
403 336.1896 670.3646 670.3762 -17.3 0 22 0.65 +1Score > 37 indicates identity
Score > 32 indicates homology		 AGPTVAR
3022 954.0017 1905.9888 1905.9870 0.98 1 22 0.62 +1Score > 39 indicates identity
Score > 32 indicates homology		 LSMIGSGVALDPYALVRE + Oxidation (M)
440 343.7139 685.4132 685.4235 -14.9 1 22 0.66 +1Score > 42 indicates identity
Score > 32 indicates homology		 NAVVKR
2650 855.4508 1708.8870 1708.8957 -5.06 1 21 0.76 +1Score > 39 indicates identity
Score > 33 indicates homology		 IEIPEPVIDMAIEPK + Oxidation (M)
1517 584.8218 1167.6290 1167.6387 -8.24 1 21 0.36 +1Score > 39 indicates identity
Score > 30 indicates homology		 EIIKPTLPQE + Deamidated (NQ)
2400 803.9254 1605.8362 1605.8436 -4.59 1 21 0.16 +1Score > 40 indicates identity
Score > 26 indicates homology		 YQLVIEGMVLAGQGK + Deamidated (NQ)
846 437.2159 872.4172 872.4174 -0.21 0 21 0.53 +1Score > 39 indicates identity
Score > 31 indicates homology		 SVLDHMR + Oxidation (M)
1305 538.3165 1074.6184 1074.6046 12.8 1 21 0.2 +1Score > 37 indicates identity
Score > 27 indicates homology		 NGHLIRHTK
1563 593.8217 1185.6288 1185.6353 -5.45 0 21 0.78 +1Score > 41 indicates identity
Score > 33 indicates homology		 QQLINLAATGR + 2 Deamidated (NQ)
1967 696.3300 1390.6454 1390.6688 -16.8 1 21 0.6 +1Score > 40 indicates identity
Score > 32 indicates homology		 SVSNEGLTLNNSR + Deamidated (NQ)
589 380.2542 758.4938 758.4902 4.85 0 21 0.39 +1Score > 30 indicates identity VSISLLK
2799 592.6525 1774.9357 1774.9505 -8.36 1 21 0.084 +1Score > 39 indicates identity
Score > 23 indicates homology		 KSLDPLYNQVLLFPE
1765 645.3506 1288.6866 1288.6775 7.08 1 21 0.89 +1Score > 41 indicates identity
Score > 33 indicates homology		 DPQAIDIYAKR
2014 707.9116 1413.8086 1413.8191 -7.41 0 21 0.67 +1Score > 36 indicates identity
Score > 32 indicates homology		 LLQQGITLGITTR + Deamidated (NQ)
1019 314.8510 941.5312 941.5294 1.86 1 21 0.34 +1Score > 39 indicates identity
Score > 29 indicates homology		 EAVQGLAVR
1022 471.7757 941.5368 941.5546 -18.8 1 21 0.55 +1Score > 40 indicates identity
Score > 31 indicates homology		 GVENVILAK
1826 331.1997 1320.7697 1320.7765 -5.17 1 21 0.18 +1Score > 33 indicates identity
Score > 26 indicates homology		 KPTSHILVEVAK
1261 353.5280 1057.5622 1057.5768 -13.8 1 21 0.63 +1Score > 42 indicates identity
Score > 32 indicates homology		 NTVDVEILR
305 603.3480 602.3407 602.3500 -15.4 1 21 0.35 +1Score > 43 indicates identity
Score > 29 indicates homology		 KGGSVR
1891 682.3718 1362.7290 1362.7064 16.6 1 21 0.43 +1Score > 40 indicates identity
Score > 30 indicates homology		 KQQLMTQISGVK + 3 Deamidated (NQ)
1519 585.3398 1168.6650 1168.6816 -14.1 1 21 0.78 +1Score > 38 indicates identity
Score > 33 indicates homology		 KIVIVGGGAGGLE
2670 859.4598 1716.9050 1716.9331 -16.4 1 21 0.5 +1Score > 39 indicates identity
Score > 31 indicates homology		 ALMQLTTKATTQIAPK + 2 Deamidated (NQ)
330 311.6823 621.3500 621.3486 2.30 0 21 0.47 +1Score > 38 indicates identity
Score > 30 indicates homology		 ATGFVK
1707 629.8671 1257.7196 1257.7081 9.16 0 21 0.55 +1Score > 37 indicates identity
Score > 31 indicates homology		 LTPFLGVLQDR
2401 803.9275 1605.8404 1605.8436 -1.97 1 21 0.3 +1Score > 39 indicates identity
Score > 28 indicates homology		 YQLVIEGMVLAGQGK + Deamidated (NQ)
538 732.4019 731.3946 731.3926 2.76 0 21 0.78 +1Score > 44 indicates identity
Score > 32 indicates homology		 TTGQGIR
1062 478.7829 955.5512 955.5451 6.48 1 21 0.59 +1Score > 40 indicates identity
Score > 31 indicates homology		 GIQKIPSGR + Deamidated (NQ)
1843 667.8602 1333.7058 1333.6990 5.14 1 21 0.45 +1Score > 40 indicates identity
Score > 30 indicates homology		 SFLVNLDRNQK + Deamidated (NQ)
1197 342.8571 1025.5495 1025.5368 12.3 1 21 0.29 +1Score > 39 indicates identity
Score > 28 indicates homology		 AFKFPTMGK
2594 839.4216 1676.8286 1676.8477 -11.4 0 21 0.18 +1Score > 41 indicates identity
Score > 26 indicates homology		 AITQSLTPSMVIMPR + Deamidated (NQ); 2 Oxidation (M)
2476 545.6111 1633.8115 1633.7981 8.16 0 21 0.26 +1Score > 41 indicates identity
Score > 28 indicates homology		 TLDSGMAGDNVGVLLR + Deamidated (NQ); Oxidation (M)
3236 680.6786 2039.0140 2038.9901 11.7 1 21 0.12 +1Score > 40 indicates identity
Score > 24 indicates homology		 GFGFITPESGNDLFVHFR
3352 1056.0449 2110.0752 2110.0946 -9.18 1 21 0.63 +1Score > 39 indicates identity
Score > 31 indicates homology		 AGQDIQEVIDNLPINVPFK + Deamidated (NQ)
793 426.2128 850.4110 850.3967 16.9 1 21 0.39 +1Score > 42 indicates identity
Score > 29 indicates homology		 SAMDKGAR + Oxidation (M)
715 411.2490 820.4834 820.4807 3.41 1 21 0.42 +1Score > 37 indicates identity
Score > 30 indicates homology		 IKQLYR + Deamidated (NQ)
1545 589.8140 1177.6134 1177.6091 3.65 1 21 0.12 +1Score > 41 indicates identity
Score > 24 indicates homology		 RGTAVTFLGQE
1911 456.5702 1366.6888 1366.6915 -1.98 0 21 0.32 +1Score > 40 indicates identity
Score > 28 indicates homology		 GTYVCIATNALGK
4352 756.3755 3021.4729 3021.4616 3.73 1 21 0.19 +1Score > 37 indicates identity
Score > 26 indicates homology		 AHNIFCPAYDCFQLVPVDIIESVVSK + Deamidated (NQ)
1319 362.1936 1083.5590 1083.5560 2.72 0 21 0.6 +1Score > 39 indicates identity
Score > 31 indicates homology		 GSAPGQPVTAAK + Deamidated (NQ)
1382 558.3036 1114.5926 1114.5982 -5.00 0 21 0.7 +1Score > 41 indicates identity
Score > 32 indicates homology		 AIDLSIDNVR
1031 316.1793 945.5161 945.5131 3.14 0 21 0.32 +1Score > 41 indicates identity
Score > 28 indicates homology		 QITATSPTK
1472 384.8498 1151.5276 1151.5241 3.05 1 21 0.27 +1Score > 41 indicates identity
Score > 28 indicates homology		 AEINSGTAMSR + Oxidation (M)
1659 411.5733 1231.6981 1231.6999 -1.45 1 21 0.18 +1Score > 38 indicates identity
Score > 26 indicates homology		 VLFELMPVIR + Oxidation (M)
1450 380.8907 1139.6503 1139.6299 17.9 1 21 0.52 +1Score > 37 indicates identity
Score > 30 indicates homology		 SSPQLDPLRK
1819 660.3499 1318.6852 1318.6802 3.80 1 21 0.82 +1Score > 41 indicates identity
Score > 32 indicates homology		 QLVSQIDLMKE + Oxidation (M)
2394 801.4277 1600.8408 1600.8474 -4.09 1 21 0.056 +1Score > 40 indicates identity
Score > 21 indicates homology		 HRWVIYTQGSNLK
1655 410.9017 1229.6833 1229.6802 2.53 1 21 0.2 +1Score > 40 indicates identity
Score > 26 indicates homology		 AMSLEKPSIVR
829 430.7487 859.4828 859.4949 -14.0 1 21 0.96 +1Score > 41 indicates identity
Score > 33 indicates homology		 LKMNLNK
762 421.2674 840.5202 840.5181 2.54 1 20 0.97 +1Score > 36 indicates identity
Score > 33 indicates homology		 KLSPLQR
2026 711.9091 1421.8036 1421.8282 -17.3 1 20 0.21 +1Score > 36 indicates identity
Score > 26 indicates homology		 KPKSDLLAFLYK
2351 790.4482 1578.8818 1578.8729 5.65 1 20 0.4 +1Score > 36 indicates identity
Score > 29 indicates homology		 ELQANVVHLSLLSR + Deamidated (NQ)
802 427.2022 852.3898 852.3865 3.94 1 20 0.92 +1Score > 41 indicates identity
Score > 33 indicates homology		 DADYLEK
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