MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3501–3600 (out of 3698)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4654 713.9463 3564.6951
4657 893.4874 3569.9205
4659 715.1985 3570.9561
4660 715.2057 3570.9921
4663 894.4990 3573.9669
4664 894.7453 3574.9521
4666 903.4754 3609.8725
4667 903.4769 3609.8785
4668 603.3320 3613.9483
4672 908.4950 3629.9509
4673 909.4631 3633.8233
4674 911.7346 3642.9093
4675 913.2242 3648.8677
4676 914.0192 3652.0477
4677 914.7175 3654.8409
4678 917.4800 3665.8909
4680 919.4629 3673.8225
4683 921.5012 3681.9757
4684 737.5754 3682.8406
4686 927.7234 3706.8645
4687 931.7571 3722.9993
4689 934.4782 3733.8837
4691 937.5129 3746.0225
4692 938.5241 3750.0673
4693 939.5184 3754.0445
4695 942.4879 3765.9225
4699 949.4962 3793.9557
4701 953.0334 3808.1045
4702 953.4409 3809.7345
4705 766.1923 3825.9251
4707 957.7599 3827.0105
4709 958.5220 3830.0589
4714 971.4965 3881.9569
4717 973.5054 3889.9925
4718 976.0057 3899.9937
4720 979.7126 3914.8213
4722 981.5264 3922.0765
4723 984.7545 3934.9889
4724 788.3998 3936.9626
4727 1000.9996 3999.9693
4729 1002.0098 4004.0101
4730 1002.0291 4004.0873
4731 801.8277 4004.1021
4732 1002.2548 4004.9901
4733 1002.2558 4004.9941
4734 1002.2755 4005.0729
4735 1002.2781 4005.0833
4736 1002.2798 4005.0901
4737 1002.2807 4005.0937
4738 1002.2811 4005.0953
4739 802.0269 4005.0981
4740 1002.2894 4005.1285
4741 1002.3018 4005.1781
4742 1002.5407 4006.1337
4743 1002.5420 4006.1389
4744 1003.7405 4010.9329
4745 803.3950 4011.9386
4746 1003.9928 4011.9421
4747 803.3967 4011.9471
4748 803.3969 4011.9481
4749 1005.7200 4018.8509
4750 804.7839 4018.8831
4752 1008.0624 4028.2205
4753 1011.4960 4041.9549
4755 1015.5287 4058.0857
4757 1016.5273 4062.0801
4758 1016.5289 4062.0865
4759 1016.5364 4062.1165
4760 1016.5371 4062.1193
4761 1016.5380 4062.1229
4762 813.4329 4062.1281
4763 1016.5407 4062.1337
4764 1016.7781 4063.0833
4765 1016.7819 4063.0985
4766 1016.7822 4063.0997
4767 814.5995 4067.9611
4768 1018.2463 4068.9561
4770 1018.2505 4068.9729
4771 1022.3127 4085.2217
4773 820.2214 4096.0706
4775 1030.5336 4118.1053
4776 1030.5446 4118.1493
4777 1030.7826 4119.1013
4778 1030.7855 4119.1129
4779 826.2051 4125.9891
4780 1034.5546 4134.1893
4783 1037.0330 4144.1029
4784 831.4387 4152.1571
4788 1040.5413 4158.1361
4789 1044.0564 4172.1965
4790 1045.5676 4178.2413
4791 1047.0071 4183.9993
4792 839.0447 4190.1871
4795 1051.5314 4202.0965
4796 1052.8120 4207.2189
4797 1064.0154 4252.0325
4799 1070.5475 4278.1609
4801 1082.5854 4326.3125
4804 870.2288 4346.1076
4805 1094.3154 4373.2325
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