MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3401–3500 (out of 3698)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4440 788.4030 3149.5829
4441 788.4115 3149.6169
4442 1051.5511 3151.6315
4452 1057.9523 3170.8351
4461 796.3914 3181.5365
4465 1061.5240 3181.5502
4466 1061.5267 3181.5583
4467 1061.5272 3181.5598
4468 1061.5337 3181.5793
4470 1061.5640 3181.6702
4472 1061.5747 3181.7023
4475 1062.5675 3184.6807
4477 797.6564 3186.5965
4480 1064.6134 3190.8184
4482 1066.2329 3195.6769
4489 641.5587 3202.7571
4491 802.1664 3204.6365
4493 1070.2273 3207.6601
4494 1070.2281 3207.6625
4495 1070.5560 3208.6462
4497 803.4055 3209.5929
4499 803.4112 3209.6157
4501 1071.9098 3212.7076
4502 1071.9098 3212.7076
4504 1074.8865 3221.6377
4505 1075.1943 3222.5611
4506 1075.5283 3223.5631
4508 1077.5480 3229.6222
4509 1077.5481 3229.6225
4511 1078.2479 3231.7219
4512 1078.8297 3233.4673
4513 1079.5284 3235.5634
4524 1082.5406 3244.6000
4525 1082.9309 3245.7709
4527 1084.2646 3249.7720
4528 813.4586 3249.8053
4529 813.4595 3249.8089
4530 813.6555 3250.5929
4531 813.7029 3250.7825
4532 1085.2589 3252.7549
4533 652.5506 3257.7166
4535 1087.5525 3259.6357
4536 1088.2054 3261.5944
4537 816.4181 3261.6433
4538 1088.5361 3262.5865
4540 1089.6284 3265.8634
4543 819.6535 3274.5849
4546 821.1533 3280.5841
4549 1099.7833 3296.3281
4550 1100.5691 3298.6855
4551 826.4068 3301.5981
4552 826.6583 3302.6041
4553 1103.2681 3306.7825
4554 827.7043 3306.7881
4556 827.7092 3306.8077
4558 827.9650 3307.8309
4561 1104.5682 3310.6828
4566 1107.5970 3319.7692
4567 1108.6008 3322.7806
4569 1112.5851 3334.7335
4570 1113.5947 3337.7623
4574 836.2150 3340.8309
4577 669.3598 3341.7626
4580 836.6664 3342.6365
4581 838.2224 3348.8605
4582 1118.3098 3351.9076
4583 838.9875 3351.9209
4584 840.2131 3356.8233
4587 841.9534 3363.7845
4589 842.4598 3365.8101
4590 1124.5945 3370.7617
4591 1125.2468 3372.7186
4592 844.4373 3373.7201
4593 676.7547 3378.7371
4595 1128.5736 3382.6990
4596 1130.9240 3389.7502
4602 683.5933 3412.9301
4604 1143.9191 3428.7355
4605 1143.9207 3428.7403
4612 1148.2855 3441.8347
4613 1149.5753 3445.7041
4619 1158.1885 3471.5437
4622 1159.8760 3476.6062
4623 1163.2449 3486.7129
4626 872.7066 3486.7973
4630 874.7388 3494.9261
4636 1171.6158 3511.8256
4637 878.9661 3511.8353
4638 706.1694 3525.8106
4639 882.4924 3525.9405
4640 882.4990 3525.9669
4641 883.9794 3531.8885
4642 1178.6362 3532.8868
4643 1178.6376 3532.8910
4644 1180.2622 3537.7648
4645 1181.6423 3541.9051
4647 887.7294 3546.8885
4650 711.9365 3554.6461
4652 890.4907 3557.9337
4653 891.4431 3561.7433
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