MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3301–3400 (out of 3698)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4113 910.8527 2729.5363
4114 910.8561 2729.5465
4123 913.5065 2737.4977
4124 915.1472 2742.4198
4125 916.4874 2746.4404
4127 916.8456 2747.5150
4128 917.5217 2749.5433
4130 917.8458 2750.5156
4131 917.8470 2750.5192
4132 917.8476 2750.5210
4133 917.8496 2750.5270
4134 918.1844 2751.5314
4138 690.3711 2757.4553
4141 921.1531 2760.4375
4148 924.5293 2770.5661
4159 929.4454 2785.3144
4160 929.4958 2785.4656
4165 930.5074 2788.5004
4169 931.1963 2790.5671
4172 931.5334 2791.5784
4174 932.1823 2793.5251
4181 936.8448 2807.5126
4194 939.5167 2815.5283
4198 941.1249 2820.3529
4200 941.5058 2821.4956
4205 943.1615 2826.4627
4212 948.5457 2842.6153
4219 951.4517 2851.3333
4222 951.5549 2851.6429
4223 952.5309 2854.5709
4230 956.1602 2865.4588
4231 956.2175 2865.6307
4235 958.1912 2871.5518
4239 720.4006 2877.5733
4243 721.1530 2880.5829
4259 967.8511 2900.5315
4262 968.5178 2902.5316
4265 970.1446 2907.4120
4267 970.4768 2908.4086
4268 970.4866 2908.4380
4269 728.1322 2908.4997
4270 971.4388 2911.2946
4273 974.2103 2919.6091
4275 731.3937 2921.5457
4278 976.4991 2926.4755
4279 976.5253 2926.5541
4280 976.8395 2927.4967
4285 737.8721 2947.4593
4288 984.5313 2950.5721
4290 985.5287 2953.5643
4291 739.8844 2955.5085
4292 739.8844 2955.5085
4294 739.8882 2955.5237
4296 739.8910 2955.5349
4297 740.1359 2956.5145
4308 990.1912 2967.5518
4309 990.5190 2968.5352
4311 990.5367 2968.5883
4312 991.5163 2971.5271
4314 995.5177 2983.5313
4321 998.4896 2992.4470
4331 998.5012 2992.4818
4340 1003.5107 3007.5103
4342 1004.1912 3009.5518
4345 1005.5493 3013.6261
4347 1006.2322 3015.6748
4348 1006.2422 3015.7048
4350 755.1810 3016.6949
4351 756.0955 3020.3529
4356 1010.5577 3028.6513
4357 1010.5587 3028.6543
4360 760.1841 3036.7073
4361 1015.8708 3044.5906
4363 1017.5001 3049.4785
4364 1017.5013 3049.4821
4365 1017.5424 3049.6054
4366 1017.5645 3049.6717
4368 1018.5445 3052.6117
4374 1021.5234 3061.5484
4380 767.8548 3067.3901
4383 1023.8484 3068.5234
4385 1024.2352 3069.6838
4386 770.1865 3076.7169
4392 1030.8467 3089.5183
4394 773.6691 3090.6473
4395 773.9198 3091.6501
4406 1034.2371 3099.6895
4408 1035.8340 3104.4802
4416 1040.8894 3119.6464
4420 1041.5619 3121.6639
4422 1043.2520 3126.7342
4426 1045.8585 3134.5537
4431 1047.5620 3139.6642
4433 788.1466 3148.5573
4434 788.1552 3148.5917
4435 788.1645 3148.6289
4436 1050.5544 3148.6414
4437 788.3934 3149.5445
4438 788.3969 3149.5585
4439 788.4027 3149.5817
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