MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3201–3300 (out of 3698)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2865 905.5193 1809.0240
2868 604.3557 1810.0453
2884 606.9581 1817.8525
2945 465.4628 1857.8221
3044 959.5078 1917.0010
3126 992.5800 1983.1454
3139 998.0913 1994.1680
3146 999.4920 1996.9694
3163 1004.5831 2007.1516
3169 1005.5583 2009.1020
3181 671.6833 2012.0281
3202 1012.5275 2023.0404
3216 678.6965 2033.0677
3235 1020.4979 2038.9812
3238 681.0325 2040.0757
3280 1037.1250 2072.2354
3282 692.0687 2073.1843
3296 693.4114 2077.2124
3297 520.3114 2077.2165
3309 696.0140 2085.0202
3325 1047.5509 2093.0872
3347 703.6845 2108.0317
3350 1055.5214 2109.0282
3387 708.4030 2122.1872
3400 710.4200 2128.2382
3407 712.0502 2133.1288
3427 715.0845 2142.2317
3441 716.7697 2147.2873
3442 1074.6541 2147.2936
3465 1079.8126 2157.6106
3502 726.4125 2176.2157
3603 748.4179 2242.2319
3604 748.4185 2242.2337
3612 1124.6361 2247.2576
3617 751.4256 2251.2550
3626 1128.6073 2255.2000
3632 754.3933 2260.1581
3633 754.3949 2260.1629
3636 1131.5808 2261.1470
3672 574.3120 2293.2189
3675 765.4245 2293.2517
3679 766.0562 2295.1468
3722 770.4502 2308.3288
3726 771.0570 2310.1492
3732 773.7590 2318.2552
3737 774.1092 2319.3058
3749 777.4464 2329.3174
3750 777.6920 2330.0542
3752 779.4146 2335.2220
3753 584.8132 2335.2237
3754 585.0368 2336.1181
3755 779.7151 2336.1235
3764 781.1096 2340.3070
3770 782.7947 2345.3623
3772 784.4603 2350.3591
3778 591.0112 2360.0157
3808 794.7578 2381.2516
3810 795.4430 2383.3072
3817 1195.2095 2388.4044
3827 600.3173 2397.2401
3830 800.4542 2398.3408
3844 604.3298 2413.2901
3845 805.8047 2414.3923
3847 806.4099 2416.2079
3860 813.1025 2436.2857
3875 817.7676 2450.2810
3877 820.1124 2457.3154
3878 820.7579 2459.2519
3888 823.1304 2466.3694
3889 823.1313 2466.3721
3911 830.7974 2489.3704
3917 832.4284 2494.2634
3923 833.7295 2498.1667
3931 837.0901 2508.2485
3935 837.4507 2509.3303
3941 839.1366 2514.3880 2514.3880 0.00080 1     +0Score > 32 indicates identity
Score > 13 indicates homology		 VFLSLPCVLNGNGLTSVINQKLK + Deamidated (NQ)
3984 858.7125 2573.1157
3986 859.8315 2576.4727
3992 861.1161 2580.3265
3993 861.1428 2580.4066
3994 861.1487 2580.4243
3997 861.4868 2581.4386
4013 652.5994 2606.3685
4033 879.4168 2635.2286
4034 879.4174 2635.2304
4036 880.4657 2638.3753
4048 884.4899 2650.4479
4053 887.1788 2658.5146
4057 889.7972 2666.3698
4058 890.1556 2667.4450
4059 890.8245 2669.4517
4061 669.3015 2673.1769
4064 893.1479 2676.4219
4066 894.4796 2680.4170
4068 895.4687 2683.3843
4069 895.4780 2683.4122
4071 672.0678 2684.2421
4077 898.4244 2692.2514
4102 903.8071 2708.3995
4106 904.4767 2710.4083
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