MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3101–3200 (out of 3698)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
183 443.2492 442.2419
184 444.2941 443.2868
185 444.2943 443.2870
186 444.3190 443.3117
188 446.2740 445.2667
189 446.2988 445.2915
190 450.2472 449.2399
191 450.2724 449.2651
192 451.2676 450.2603
193 451.2679 450.2606
195 466.2427 465.2354
196 467.7737 466.7664
198 472.3268 471.3195
206 476.2114 475.2041
207 480.7645 479.7572
208 481.7763 480.7690
209 484.3284 483.3211
210 484.3287 483.3214
211 484.3288 483.3215
216 493.2787 492.2714
223 506.3101 505.3028
224 508.2890 507.2817
225 510.2376 509.2303
230 516.3135 515.3062
233 522.7798 521.7725
234 524.2370 523.2297
235 524.3098 523.3025
245 533.1208 532.1135
248 538.3157 537.3084
254 550.3007 549.2934
255 552.8260 551.8187
256 555.1038 554.0965
258 557.0791 556.0718
262 559.8188 558.8115
263 566.2949 565.2876
265 569.8051 568.7978
266 571.0677 570.0604
267 571.8585 570.8512
274 581.8284 580.8211
285 586.8321 585.8248
294 599.6761 598.6688
295 600.0101 599.0028
319 614.8373 613.8300
324 618.3572 617.3499
335 626.3162 625.3089
336 626.4210 625.4137
453 692.3840 691.3767
494 357.2140 712.4134
545 734.7263 733.7190
571 750.0923 749.0850
575 752.9005 751.8932
695 807.9429 806.9356
696 808.1221 807.1148
748 833.9116 832.9043
753 837.1236 836.1163
810 855.0719 854.0646
843 872.4432 871.4359
862 880.9544 879.9471
872 443.2939 884.5732
921 903.4798 902.4725
938 912.8011 911.7938
965 923.1498 922.1425
967 924.4796 923.4723
1003 939.9734 938.9661
1072 480.3279 958.6412
1215 1033.2139 1032.2066
1234 1040.5527 1039.5454
1252 1053.5184 1052.5111
1404 1124.1279 1123.1206
1406 1125.1339 1124.1266
1445 569.3566 1136.6986
1464 382.9259 1145.7559
1591 600.0679 1198.1212
1656 615.9116 1229.8086
1834 443.2664 1326.7774
2022 711.8265 1421.6384
2040 713.1464 1424.2782
2072 481.9954 1442.9644
2101 489.6330 1465.8772
2143 495.0081 1482.0025
2160 498.6290 1492.8652
2219 756.6498 1511.2850
2303 779.9257 1557.8368
2334 525.3208 1572.9406
2335 787.4779 1572.9412
2336 787.4786 1572.9426
2362 791.9148 1581.8150
2471 408.7465 1630.9569
2474 327.3990 1631.9586
2586 419.0092 1672.0077
2592 335.6094 1673.0106
2605 843.9462 1685.8778
2622 848.4898 1694.9650
2664 572.2838 1713.8296
2715 578.6557 1732.9453
2734 873.0016 1743.9886
2746 586.2486 1755.7240
2769 589.2958 1764.8656
2789 885.8638 1769.7130
2791 443.6861 1770.7153
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