MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 201–300 (out of 3698)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1822 660.8284 1319.6422 1319.6622 -15.1 1 26 0.37 +1Score > 41 indicates identity
Score > 34 indicates homology
INQQAFQWKR + 2 Deamidated (NQ)
709 408.2548 814.4950 814.4800 18.5 1 26 0.46 +1Score > 40 indicates identity
Score > 35 indicates homology
IELIAQK + Deamidated (NQ)
3780 787.7946 2360.3620 2360.3427 8.17 1 26 0.091 +1Score > 28 indicates identity RPLVVAGTSLADPALIEAAANIAK
2238 506.9532 1517.8378 1517.8202 11.6 1 26 0.26 +1Score > 38 indicates identity
Score > 32 indicates homology
VKFNVINTAVQGAR + 2 Deamidated (NQ)
388 331.6856 661.3566 661.3581 -2.21 1 26 0.29 +1Score > 42 indicates identity
Score > 33 indicates homology
AAGMKGK
236 529.3123 528.3050 528.3020 5.72 0 26 0.13 +1Score > 38 indicates identity
Score > 29 indicates homology
GPVTR
983 465.2774 928.5402 928.5454 -5.53 1 26 0.49 +1Score > 41 indicates identity
Score > 35 indicates homology
IINSARQK
1026 472.2664 942.5182 942.5134 5.11 1 26 0.71 +1Score > 41 indicates identity
Score > 37 indicates homology
GLGIEDALR
924 452.7679 903.5212 903.5178 3.84 0 26 0.16 +1Score > 38 indicates identity
Score > 30 indicates homology
AHPLPLQK + Deamidated (NQ)
442 687.3805 686.3732 686.3711 3.04 0 26 1 +1Score > 44 indicates identity
Score > 38 indicates homology
GVDVAAR
1803 656.3928 1310.7710 1310.7922 -16.1 1 26 0.48 +1Score > 35 indicates identity VDKDILAGVIIR
572 375.7247 749.4348 749.4323 3.36 1 25 0.36 +1Score > 36 indicates identity
Score > 34 indicates homology
FITLEK
1414 565.2885 1128.5624 1128.5559 5.82 1 25 0.7 +1Score > 40 indicates identity
Score > 36 indicates homology
MSYKLMLVE + Oxidation (M)
227 516.2921 515.2848 515.2816 6.28 0 25 0.63 +1Score > 40 indicates identity
Score > 36 indicates homology
AGVNR
1995 469.5993 1405.7761 1405.7929 -12.0 0 25 0.068 +1Score > 38 indicates identity
Score > 26 indicates homology
ITPLVPVAASGQPR + Deamidated (NQ)
413 673.3647 672.3574 672.3555 2.91 1 25 0.57 +1Score > 44 indicates identity
Score > 35 indicates homology
EQGAIR
988 466.2597 930.5048 930.5022 2.87 1 25 0.78 +1Score > 42 indicates identity
Score > 37 indicates homology
IISEDINK
2705 866.9415 1731.8684 1731.8753 -3.96 0 25 0.5 +1Score > 41 indicates identity
Score > 35 indicates homology
IDIAILPIGGNFTMGAE + Deamidated (NQ)
911 450.2908 898.5670 898.5600 7.86 0 25 0.46 +1Score > 34 indicates identity QLGSIIIR
1185 508.7740 1015.5334 1015.5338 -0.39 0 25 0.61 +1Score > 42 indicates identity
Score > 36 indicates homology
IGGIPLFNGE
2232 758.9332 1515.8518 1515.8344 11.5 1 25 0.27 +1Score > 36 indicates identity
Score > 32 indicates homology
LGLMIAIRYSHSR
197 471.2692 470.2619 470.2601 3.84 0 25 0.19 +1Score > 30 indicates identity APGAR
635 390.2069 778.3992 778.4007 -1.86 1 25 0.91 +1Score > 43 indicates identity
Score > 37 indicates homology
KITNMR + Deamidated (NQ); Oxidation (M)
1296 536.7878 1071.5610 1071.5560 4.71 1 25 0.78 +1Score > 42 indicates identity
Score > 36 indicates homology
AGASNPKQAVK + 2 Deamidated (NQ)
1513 388.2095 1161.6067 1161.6281 -18.5 1 25 0.16 +1Score > 41 indicates identity
Score > 30 indicates homology
FLQLAVDKVE + Deamidated (NQ)
368 323.6927 645.3708 645.3632 11.8 0 25 0.49 +1Score > 43 indicates identity
Score > 34 indicates homology
AGMIVR
990 466.2658 930.5170 930.5069 10.9 1 25 0.81 +1Score > 42 indicates identity
Score > 37 indicates homology
QAAMVQKR
2135 739.8992 1477.7838 1477.7777 4.18 1 25 0.57 +1Score > 39 indicates identity
Score > 35 indicates homology
QGFLSVATLRVGTE + Deamidated (NQ)
1730 425.5435 1273.6087 1273.6262 -13.8 1 25 0.38 +1Score > 41 indicates identity
Score > 33 indicates homology
QATQRNAVNIR + 4 Deamidated (NQ)
576 377.2171 752.4196 752.4181 2.09 0 25 0.15 +1Score > 38 indicates identity
Score > 29 indicates homology
ALDVHAK
309 303.1460 604.2774 604.2704 11.7 0 25 0.82 +1Score > 44 indicates identity
Score > 37 indicates homology
ANSAIE + Deamidated (NQ)
411 336.7121 671.4096 671.3966 19.4 1 25 0.78 +1Score > 44 indicates identity
Score > 36 indicates homology
GVVEIR
543 367.7147 733.4148 733.4123 3.53 0 25 0.8 +1Score > 40 indicates identity
Score > 36 indicates homology
FADILR
871 884.4401 883.4328 883.4300 3.15 0 25 0.18 +1Score > 38 indicates identity
Score > 30 indicates homology
AHAFAPDR
2166 748.4516 1494.8886 1494.8770 7.80 0 25 0.06 +1Score > 32 indicates identity
Score > 25 indicates homology
LAADVTGVPTLLVAR
1167 503.2969 1004.5792 1004.5767 2.55 1 25 0.67 +1Score > 37 indicates identity
Score > 36 indicates homology
RLFITAQR + Deamidated (NQ)
1223 1035.5863 1034.5790 1034.5913 -11.9 1 25 0.21 +1Score > 38 indicates identity
Score > 31 indicates homology
FAKGLPGFAK
412 336.7136 671.4126 671.4078 7.17 1 25 0.91 +1Score > 42 indicates identity
Score > 37 indicates homology
RVQIR + Deamidated (NQ)
1526 586.3070 1170.5994 1170.5993 0.16 1 25 0.17 +1Score > 40 indicates identity
Score > 29 indicates homology
DQLNAAEIAAR
2420 806.9015 1611.7884 1611.7933 -2.98 0 25 0.056 +1Score > 40 indicates identity
Score > 25 indicates homology
ANFLAISGYIWNNK + 2 Deamidated (NQ)
707 408.2351 814.4556 814.4548 0.98 1 25 0.71 +1Score > 43 indicates identity
Score > 36 indicates homology
EIIIDGR
645 393.7277 785.4408 785.4396 1.64 0 24 0.85 +1Score > 43 indicates identity
Score > 36 indicates homology
VAGLGDVR
1325 543.3235 1084.6324 1084.6240 7.77 0 24 0.45 +1Score > 39 indicates identity
Score > 33 indicates homology
LDAATLAAALR
2111 490.9283 1469.7631 1469.7627 0.27 1 24 0.51 +1Score > 40 indicates identity
Score > 34 indicates homology
HSVYGGVVPELASR
1208 1031.5146 1030.5073 1030.4944 12.5 1 24 0.87 +1Score > 41 indicates identity
Score > 36 indicates homology
NFKNGHASR + Deamidated (NQ)
441 344.1936 686.3726 686.3711 2.24 1 24 0.83 +1Score > 44 indicates identity
Score > 36 indicates homology
AIAEGAR
781 848.3920 847.3847 847.3937 -10.5 1 24 0.6 +1Score > 41 indicates identity
Score > 34 indicates homology
DDTWRR
892 447.7388 893.4630 893.4607 2.67 1 24 0.38 +1Score > 41 indicates identity
Score > 32 indicates homology
LSGELYGR
1387 373.8545 1118.5417 1118.5455 -3.44 0 24 0.94 +1Score > 41 indicates identity
Score > 36 indicates homology
NTVTGDQAAIK + 2 Deamidated (NQ)
301 301.6854 601.3562 601.3548 2.49 0 24 0.76 +1Score > 43 indicates identity
Score > 35 indicates homology
IGGSIR
2595 839.9553 1677.8960 1677.8937 1.38 0 24 0.096 +1Score > 38 indicates identity
Score > 27 indicates homology
LQDIGNGLQPAVPGGIK + 2 Deamidated (NQ)
1759 642.8668 1283.7190 1283.6946 19.0 1 24 0.63 +1Score > 38 indicates identity
Score > 35 indicates homology
DNVVAALVRGDR
395 666.3594 665.3521 665.3497 3.69 0 24 0.79 +1Score > 39 indicates identity
Score > 36 indicates homology
QYVTR
352 317.2085 632.4024 632.4010 2.31 0 24 0.092 +1Score > 28 indicates identity
Score > 26 indicates homology
FIVVR
308 302.6877 603.3608 603.3592 2.80 0 24 0.64 +1Score > 40 indicates identity
Score > 35 indicates homology
ISSGLK
891 894.4703 893.4630 893.4607 2.64 1 24 0.99 +1Score > 41 indicates identity
Score > 36 indicates homology
GISEGLYR
407 336.2271 670.4396 670.4377 2.84 0 24 0.86 +1Score > 37 indicates identity
Score > 36 indicates homology
AVAAIVK
1221 518.2792 1034.5438 1034.5284 14.9 1 24 0.82 +1Score > 41 indicates identity
Score > 36 indicates homology
AIIQGKQFE + 2 Deamidated (NQ)
714 410.7335 819.4524 819.4490 4.16 0 24 0.56 +1Score > 40 indicates identity
Score > 34 indicates homology
AGNILFGK + Deamidated (NQ)
780 424.6996 847.3846 847.3937 -10.6 1 24 0.25 +1Score > 41 indicates identity
Score > 30 indicates homology
DDTWRR
985 465.7681 929.5216 929.5083 14.4 1 24 0.62 +1Score > 42 indicates identity
Score > 34 indicates homology
LGAAWQKR + Deamidated (NQ)
1841 665.3465 1328.6784 1328.6936 -11.4 1 24 0.49 +1Score > 41 indicates identity
Score > 33 indicates homology
QVVVNLLGSRGGE + 2 Deamidated (NQ)
1320 542.7869 1083.5592 1083.5560 2.97 0 24 0.15 +1Score > 39 indicates identity
Score > 28 indicates homology
GSAPGQPVTAAK + Deamidated (NQ)
795 426.7097 851.4048 851.4025 2.78 1 24 0.16 +1Score > 42 indicates identity
Score > 28 indicates homology
YGQEVQK + Deamidated (NQ)
332 622.3580 621.3507 621.3486 3.39 0 24 0.77 +1Score > 38 indicates identity
Score > 35 indicates homology
AGFTVK
1646 614.8380 1227.6614 1227.6459 12.7 1 24 0.95 +1Score > 40 indicates identity
Score > 36 indicates homology
QAQQLLELQR + 2 Deamidated (NQ)
2894 456.9959 1823.9545 1823.9279 14.6 0 24 0.91 +1Score > 38 indicates identity
Score > 36 indicates homology
DKPHVNIGTIGHVDHGK + Deamidated (NQ)
991 932.4822 931.4749 931.4797 -5.08 1 24 0.48 +1Score > 43 indicates identity
Score > 33 indicates homology
LAMQEAIR + Deamidated (NQ)
691 801.4975 800.4902 800.4868 4.26 1 24 0.65 +1Score > 40 indicates identity
Score > 34 indicates homology
KSIGIQR
1217 517.7491 1033.4836 1033.5040 -19.7 0 24 0.66 +1Score > 41 indicates identity
Score > 34 indicates homology
QSQNTIVSR + 2 Deamidated (NQ)
2653 856.4456 1710.8766 1710.8788 -1.25 1 24 0.54 +1Score > 39 indicates identity
Score > 33 indicates homology
SLQAELSHITDIINR + 2 Deamidated (NQ)
2534 828.4808 1654.9470 1654.9215 15.4 1 23 0.21 +1Score > 34 indicates identity
Score > 29 indicates homology
QMLLEGLDPIALTLK + Deamidated (NQ)
918 451.7517 901.4888 901.4869 2.17 0 23 0.86 +1Score > 43 indicates identity
Score > 35 indicates homology
VTLADLDR
838 434.7204 867.4262 867.4239 2.70 0 23 0.089 +1Score > 39 indicates identity
Score > 25 indicates homology
FYPTQGR
1103 487.7723 973.5300 973.5233 6.95 0 23 0.88 +1Score > 41 indicates identity
Score > 35 indicates homology
DLDIPFVR
1307 539.2655 1076.5164 1076.5363 -18.4 1 23 0.19 +1Score > 42 indicates identity
Score > 29 indicates homology
AGQSGRTFPR + Deamidated (NQ)
3438 716.0701 2145.1885 2145.1470 19.3 1 23 0.41 +1Score > 34 indicates identity
Score > 32 indicates homology
GFDVVSGGTENHLFLLSLIK
228 516.3041 515.2968 515.2955 2.60 0 23 0.075 +1Score > 47 indicates identity
Score > 25 indicates homology
ALAQL + Deamidated (NQ)
1898 683.8281 1365.6416 1365.6452 -2.61 0 23 0.29 +1Score > 41 indicates identity
Score > 30 indicates homology
LASDLPAANFFAE + Deamidated (NQ)
788 425.7198 849.4250 849.4232 2.17 1 23 0.92 +1Score > 42 indicates identity
Score > 35 indicates homology
FLEQNAK + Deamidated (NQ)
495 357.2200 712.4254 712.4232 3.22 0 23 0.2 +1Score > 37 indicates identity
Score > 29 indicates homology
SPIIQR
1138 332.8546 995.5420 995.5440 -2.04 1 23 0.17 +1Score > 38 indicates identity
Score > 28 indicates homology
KGFAFDAIK
3079 488.7485 1950.9649 1950.9660 -0.57 1 23 0.13 +1Score > 40 indicates identity
Score > 27 indicates homology
SNRHIYAQVIDDVHQR + Deamidated (NQ)
315 307.7038 613.3930 613.3911 3.10 0 23 0.7 +1Score > 40 indicates identity
Score > 34 indicates homology
VAGVLR
792 851.4180 850.4107 850.3967 16.5 0 23 0.38 +1Score > 42 indicates identity
Score > 32 indicates homology
QASGSMVR + Oxidation (M)
590 380.7202 759.4258 759.4239 2.58 0 23 0.81 +1Score > 42 indicates identity
Score > 35 indicates homology
IGSISQR
1358 552.8276 1103.6406 1103.6186 19.9 0 23 0.19 +1Score > 37 indicates identity
Score > 28 indicates homology
INVLTTTNTK
531 729.4426 728.4353 728.4293 8.26 1 23 0.97 +1Score > 41 indicates identity
Score > 36 indicates homology
DRVIAR
2010 707.3730 1412.7314 1412.7147 11.9 0 23 0.6 +1Score > 40 indicates identity
Score > 33 indicates homology
NAVVLLNTQGEPR + 3 Deamidated (NQ)
689 401.2518 800.4890 800.4868 2.79 1 23 0.92 +1Score > 40 indicates identity
Score > 35 indicates homology
KSGIGALR
465 349.7017 697.3888 697.3759 18.6 1 23 0.63 +1Score > 37 indicates identity
Score > 34 indicates homology
TLHKAE
582 379.2332 756.4518 756.4494 3.29 1 23 0.7 +1Score > 41 indicates identity
Score > 34 indicates homology
KNASLPK
1567 594.3204 1186.6262 1186.6346 -7.02 1 23 0.79 +1Score > 41 indicates identity
Score > 34 indicates homology
AKASLIAQAWE
229 516.3046 515.2973 515.2955 3.57 0 23 0.14 +1Score > 47 indicates identity
Score > 27 indicates homology
ALAQL + Deamidated (NQ)
580 754.3537 753.3464 753.3327 18.2 1 23 0.95 +1Score > 39 indicates identity
Score > 35 indicates homology
TETTMR + Oxidation (M)
996 468.7626 935.5106 935.4964 15.2 1 23 0.74 +1Score > 40 indicates identity
Score > 34 indicates homology
DVAYVLKE
813 428.7305 855.4464 855.4450 1.67 0 23 0.18 +1Score > 38 indicates identity
Score > 28 indicates homology
LADPDVAR
1802 656.3427 1310.6708 1310.6942 -17.9 0 23 0.099 +1Score > 40 indicates identity
Score > 25 indicates homology
QNLPQIDQISR
3155 668.0444 2001.1114 2001.1106 0.38 1 23 0.055 +1Score > 36 indicates identity
Score > 23 indicates homology
GAAGSDTKILAVTILTSLDR
360 322.2114 642.4082 642.4177 -14.7 1 23 0.37 +1Score > 41 indicates identity
Score > 31 indicates homology
KGAVIR
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