MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 101–200 (out of 3698)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
746 417.2328 832.4510 832.4476 4.09 1 30 0.61 +1Score > 41 indicates identity
Score > 40 indicates homology		 LTEMLAR
1653 615.3650 1228.7154 1228.7027 10.4 1 30 0.29 +1Score > 37 indicates identity QLSEGLLALSAK
1124 494.7852 987.5558 987.5726 -17.0 1 30 0.6 +1Score > 40 indicates identity LPHPAARAR
1030 473.2491 944.4836 944.4814 2.33 1 30 0.77 +1Score > 42 indicates identity
Score > 41 indicates homology		 LDQLAAEGK + Deamidated (NQ)
1399 374.5374 1120.5904 1120.5764 12.5 0 30 0.18 +1Score > 40 indicates identity
Score > 35 indicates homology		 KPSYNVGINK + 2 Deamidated (NQ)
1424 566.2872 1130.5598 1130.5567 2.74 1 30 0.12 +1Score > 42 indicates identity
Score > 33 indicates homology		 VVELNASDQR + Deamidated (NQ)
592 381.2077 760.4008 760.4079 -9.27 0 30 0.98 +1Score > 44 indicates identity
Score > 42 indicates homology		 ATNQSIK
1705 629.8559 1257.6972 1257.6816 12.4 0 30 0.54 +1Score > 39 indicates identity LVIAVANDSNIK + 2 Deamidated (NQ)
389 331.6962 661.3778 661.3833 -8.18 0 29 0.51 +1Score > 39 indicates identity ISLMAK
667 795.3905 794.3832 794.3810 2.78 1 29 0.25 +1Score > 41 indicates identity
Score > 36 indicates homology		 GFELNSK + Deamidated (NQ)
1023 471.7919 941.5692 941.5658 3.67 0 29 0.45 +1Score > 38 indicates identity LIVGNSALR
1209 516.2616 1030.5086 1030.4931 15.1 0 29 0.11 +1Score > 41 indicates identity
Score > 32 indicates homology		 LTQNLAGGQE + Deamidated (NQ)
1564 593.8225 1185.6304 1185.6353 -4.12 0 29 0.12 +1Score > 41 indicates identity
Score > 33 indicates homology		 DALAIIDAGATR
2853 902.9692 1803.9238 1803.8924 17.4 1 29 0.061 +1Score > 40 indicates identity
Score > 30 indicates homology		 LTGSDVNLVAKNLMSPE + Deamidated (NQ); Oxidation (M)
787 425.7160 849.4174 849.4014 18.9 0 29 0.094 +1Score > 42 indicates identity
Score > 32 indicates homology		 LSMANAAR + Deamidated (NQ); Oxidation (M)
534 365.7331 729.4516 729.4497 2.68 1 29 0.081 +1Score > 41 indicates identity
Score > 31 indicates homology		 KISINR
896 894.4710 893.4637 893.4606 3.44 0 29 0.68 +1Score > 41 indicates identity
Score > 40 indicates homology		 NALSLGYR + Deamidated (NQ)
321 615.3600 614.3527 614.3500 4.45 0 29 0.66 +1Score > 40 indicates identity QAGIAR
1748 640.3138 1278.6130 1278.5979 11.8 1 29 0.077 +1Score > 41 indicates identity
Score > 30 indicates homology		 QLTIDELFNGE + Deamidated (NQ)
410 336.7115 671.4084 671.3966 17.6 1 29 0.87 +1Score > 44 indicates identity
Score > 41 indicates homology		 VGVEIR
478 352.2034 702.3922 702.3912 1.48 1 29 0.4 +1Score > 44 indicates identity
Score > 37 indicates homology		 AGEVTVK
1586 399.8959 1196.6659 1196.6625 2.80 0 29 0.46 +1Score > 38 indicates identity NSRPININLR + Deamidated (NQ)
981 929.5444 928.5371 928.5454 -8.91 1 29 0.34 +1Score > 41 indicates identity
Score > 37 indicates homology		 QVLSARQK
600 382.7327 763.4508 763.4480 3.77 0 29 0.15 +1Score > 35 indicates identity
Score > 33 indicates homology		 IIDYIK
331 311.6825 621.3504 621.3486 2.97 0 29 0.13 +1Score > 38 indicates identity
Score > 33 indicates homology		 LSAGFK
1227 1035.5902 1034.5829 1034.6012 -17.6 1 29 0.48 +1Score > 38 indicates identity IEIYIVGTK
1643 614.8373 1227.6600 1227.6823 -18.1 0 29 0.79 +1Score > 40 indicates identity IDVGGSAAVLGAAK
1595 601.3384 1200.6622 1200.6503 9.99 1 29 0.78 +1Score > 40 indicates identity SWALVDILER
1644 614.8379 1227.6612 1227.6823 -17.1 0 29 0.69 +1Score > 40 indicates identity
Score > 40 indicates homology		 IDVGGSAAVLGAAK
325 309.6958 617.3770 617.3748 3.59 0 29 0.35 +1Score > 38 indicates identity
Score > 37 indicates homology		 TAVVTK
860 440.2529 878.4912 878.4861 5.83 0 29 0.45 +1Score > 38 indicates identity
Score > 38 indicates homology		 YILNSIR + Deamidated (NQ)
414 673.4001 672.3928 672.3806 18.1 1 29 0.8 +1Score > 43 indicates identity
Score > 40 indicates homology		 QEGVLK
385 330.1960 658.3774 658.3650 18.9 0 28 0.91 +1Score > 44 indicates identity
Score > 41 indicates homology		 IQVTQA
370 324.1667 646.3188 646.3187 0.24 0 28 0.57 +1Score > 44 indicates identity
Score > 38 indicates homology		 SWQAR
432 683.4470 682.4397 682.4378 2.88 0 28 0.088 +1Score > 34 indicates identity
Score > 30 indicates homology		 VPAVIGK
2038 712.8629 1423.7112 1423.7347 -16.5 0 28 0.27 +1Score > 41 indicates identity
Score > 35 indicates homology		 LGGFFGTQQGLIGK + 2 Deamidated (NQ)
1169 503.7581 1005.5016 1005.4913 10.3 1 28 0.064 +1Score > 41 indicates identity
Score > 29 indicates homology		 MGADQRTVK + Deamidated (NQ)
916 901.5017 900.4944 900.4916 3.13 1 28 0.77 +1Score > 42 indicates identity
Score > 40 indicates homology		 EALQALGAK + Deamidated (NQ)
1565 593.8251 1185.6356 1185.6353 0.28 0 28 0.11 +1Score > 41 indicates identity
Score > 31 indicates homology		 QQLINLAATGR + 2 Deamidated (NQ)
995 468.2740 934.5334 934.5276 6.22 0 28 0.5 +1Score > 38 indicates identity NFLIPFGK
1413 564.8203 1127.6260 1127.6299 -3.39 0 28 0.25 +1Score > 40 indicates identity
Score > 35 indicates homology		 LQGQNVLTVR + Deamidated (NQ)
1648 614.8381 1227.6616 1227.6710 -7.62 1 28 0.6 +1Score > 40 indicates identity
Score > 38 indicates homology		 NILNEAQIIAK + 2 Deamidated (NQ)
578 753.4274 752.4201 752.4181 2.72 0 28 0.27 +1Score > 38 indicates identity
Score > 35 indicates homology		 ALDVHAK
1947 462.9200 1385.7382 1385.7337 3.26 0 28 0.38 +1Score > 40 indicates identity
Score > 36 indicates homology		 QITAAMQQLLPR + Deamidated (NQ); Oxidation (M)
1587 399.8962 1196.6668 1196.6625 3.55 0 28 0.26 +1Score > 38 indicates identity
Score > 34 indicates homology		 NSRPININLR + Deamidated (NQ)
772 422.7131 843.4116 843.4086 3.58 1 28 0.56 +1Score > 39 indicates identity
Score > 38 indicates homology		 QLSPEDR
887 892.4795 891.4722 891.4702 2.31 1 28 0.51 +1Score > 42 indicates identity
Score > 37 indicates homology		 LGQEGFIK + Deamidated (NQ)
1024 943.4783 942.4710 942.4631 8.37 1 28 0.79 +1Score > 39 indicates identity QRGDAGSPR
1776 432.2740 1293.8002 1293.7768 18.0 1 28 0.077 +1Score > 29 indicates identity VVGNPIRNAILK + Deamidated (NQ)
417 673.4011 672.3938 672.3918 2.94 1 28 0.78 +1Score > 43 indicates identity
Score > 39 indicates homology		 KQGLAR + Deamidated (NQ)
817 429.2489 856.4832 856.4767 7.70 0 28 0.36 +1Score > 39 indicates identity
Score > 36 indicates homology		 LVANDVAR
390 665.3636 664.3563 664.3544 2.87 1 28 0.11 +1Score > 41 indicates identity
Score > 31 indicates homology		 VEVYR
264 569.3057 568.2984 568.2969 2.67 0 28 0.66 +1Score > 38 indicates identity HVDAK
387 330.2089 658.4032 658.4014 2.86 0 28 0.89 +1Score > 40 indicates identity
Score > 40 indicates homology		 IQVTAK
2456 812.9216 1623.8286 1623.8153 8.22 0 28 0.97 +1Score > 40 indicates identity VGPFGMFQIAADMIK
379 326.6993 651.3840 651.3816 3.70 0 27 0.11 +1Score > 34 indicates identity
Score > 30 indicates homology		 AIGHVR
2415 537.6244 1609.8514 1609.8576 -3.88 1 27 0.1 +1Score > 39 indicates identity
Score > 30 indicates homology		 VTIYNHPGIQAELR
419 337.2169 672.4192 672.4170 3.31 0 27 0.86 +1Score > 40 indicates identity
Score > 39 indicates homology		 AVSLGVK
964 922.5010 921.4937 921.4920 1.90 0 27 0.6 +1Score > 40 indicates identity
Score > 38 indicates homology		 FNGSVAISK
2172 499.9331 1496.7775 1496.7947 -11.5 1 27 0.18 +1Score > 39 indicates identity
Score > 32 indicates homology		 AVRQVQAAAAALAGAE + Deamidated (NQ)
533 365.2357 728.4568 728.4545 3.29 0 27 0.6 +1Score > 42 indicates identity
Score > 37 indicates homology		 IAVSAIR
845 436.7740 871.5334 871.5378 -5.04 1 27 0.2 +1Score > 38 indicates identity
Score > 33 indicates homology		 VLKLASIE
1815 659.3435 1316.6724 1316.6572 11.6 1 27 0.63 +1Score > 41 indicates identity
Score > 38 indicates homology		 LSAQESVQQAGAK + Deamidated (NQ)
2651 570.6468 1708.9186 1708.9070 6.80 0 27 0.16 +1Score > 38 indicates identity
Score > 31 indicates homology		 TNPDAMPILVVPDLSK
445 688.3691 687.3618 687.3592 3.87 0 27 0.24 +1Score > 43 indicates identity
Score > 33 indicates homology		 APFQPK + Deamidated (NQ)
1116 491.7415 981.4684 981.4556 13.1 1 27 0.089 +1Score > 41 indicates identity
Score > 29 indicates homology		 WEQYVTR + Deamidated (NQ)
1625 609.8630 1217.7114 1217.7019 7.80 1 27 0.1 +1Score > 35 indicates identity
Score > 29 indicates homology		 FANTLLELGIK
1342 548.2604 1094.5062 1094.5179 -10.6 0 27 0.59 +1Score > 41 indicates identity
Score > 37 indicates homology		 VMNLAGFGNR + Deamidated (NQ); Oxidation (M)
699 406.2440 810.4734 810.4712 2.80 0 27 0.37 +1Score > 35 indicates identity ITIHTAR
364 322.7032 643.3918 643.4017 -15.3 0 27 0.4 +1Score > 43 indicates identity
Score > 35 indicates homology		 AIAITR
1027 472.7719 943.5292 943.5338 -4.84 1 27 0.57 +1Score > 42 indicates identity
Score > 37 indicates homology		 TLQALLER + Deamidated (NQ)
941 457.7567 913.4988 913.5093 -11.5 1 27 0.82 +1Score > 40 indicates identity
Score > 38 indicates homology		 AGISRAPSR
1159 502.2817 1002.5488 1002.5346 14.3 0 27 0.49 +1Score > 41 indicates identity
Score > 36 indicates homology		 VTNVAGINSK + Deamidated (NQ)
371 647.3278 646.3205 646.3187 2.83 0 27 0.72 +1Score > 44 indicates identity
Score > 38 indicates homology		 SWQAR
922 452.2440 902.4734 902.4821 -9.64 0 26 0.82 +1Score > 43 indicates identity
Score > 38 indicates homology		 VTAGLDTAR
532 365.2252 728.4358 728.4333 3.47 0 26 0.92 +1Score > 41 indicates identity
Score > 39 indicates homology		 IAWIAR
322 308.6876 615.3606 615.3704 -15.9 1 26 0.37 +1Score > 45 indicates identity
Score > 35 indicates homology		 AGGGKVK
615 388.1916 774.3686 774.3759 -9.39 1 26 0.52 +1Score > 42 indicates identity
Score > 36 indicates homology		 LENNGVK + 2 Deamidated (NQ)
2274 771.9285 1541.8424 1541.8235 12.3 0 26 0.2 +1Score > 38 indicates identity
Score > 32 indicates homology		 MAAAVASLATLAAEPR
980 465.2664 928.5182 928.5229 -5.03 1 26 0.18 +1Score > 41 indicates identity
Score > 32 indicates homology		 AGVQALKLE + Deamidated (NQ)
721 412.7359 823.4572 823.4552 2.49 0 26 0.24 +1Score > 38 indicates identity
Score > 33 indicates homology		 HGITPTAK
1168 503.2983 1004.5820 1004.5655 16.5 0 26 0.35 +1Score > 38 indicates identity
Score > 34 indicates homology		 TIAFGQVIR + Deamidated (NQ)
1388 560.2787 1118.5428 1118.5455 -2.37 1 26 0.32 +1Score > 42 indicates identity
Score > 34 indicates homology		 ENTASDILQK + Deamidated (NQ)
1942 692.8962 1383.7778 1383.7722 4.11 1 26 0.8 +1Score > 38 indicates identity
Score > 38 indicates homology		 SLRAIVDLQALGE
879 444.7159 887.4172 887.4348 -19.8 0 26 0.14 +1Score > 41 indicates identity
Score > 30 indicates homology		 NQQGQALK + 2 Deamidated (NQ)
1687 620.8518 1239.6890 1239.6823 5.48 0 26 0.17 +1Score > 38 indicates identity
Score > 31 indicates homology		 KPVNNIANLGAK + 2 Deamidated (NQ)
1095 486.7660 971.5174 971.5148 2.69 0 26 0.36 +1Score > 42 indicates identity
Score > 34 indicates homology		 SAGLQVQNR
362 322.2117 642.4088 642.4064 3.76 0 26 0.78 +1Score > 41 indicates identity
Score > 38 indicates homology		 AIAGLAK
612 772.4216 771.4143 771.4126 2.18 0 26 0.61 +1Score > 41 indicates identity
Score > 36 indicates homology		 AAAVEPSK
1594 601.3376 1200.6606 1200.6503 8.66 1 26 0.4 +1Score > 40 indicates identity
Score > 35 indicates homology		 SWALVDILER
1492 579.3009 1156.5872 1156.5724 12.8 1 26 0.65 +1Score > 41 indicates identity
Score > 37 indicates homology		 LNDPIDQKGR + 2 Deamidated (NQ)
2178 750.3768 1498.7390 1498.7263 8.47 0 26 0.77 +1Score > 40 indicates identity
Score > 37 indicates homology		 VPSDISQPQSGDLR + Deamidated (NQ)
778 848.3915 847.3842 847.3923 -9.55 1 26 0.71 +1Score > 41 indicates identity
Score > 37 indicates homology		 DDGTEALK
2297 778.4402 1554.8658 1554.8869 -13.5 1 26 0.53 +1Score > 37 indicates identity
Score > 36 indicates homology		 DKIIVQSVPDVTLK + Deamidated (NQ)
303 301.6863 601.3580 601.3548 5.48 0 26 0.38 +1Score > 43 indicates identity
Score > 34 indicates homology		 IGASVR
584 379.7310 757.4474 757.4334 18.6 1 26 0.13 +1Score > 43 indicates identity
Score > 30 indicates homology		 ANIIAEK
654 789.4647 788.4574 788.4578 -0.51 0 26 0.29 +1Score > 39 indicates identity
Score > 33 indicates homology		 IMGALLR + Oxidation (M)
1014 471.7513 941.4880 941.4818 6.64 0 26 0.15 +1Score > 39 indicates identity
Score > 30 indicates homology		 LASPNTDPK
599 382.7204 763.4262 763.4228 4.51 1 26 0.14 +1Score > 41 indicates identity
Score > 30 indicates homology		 IIAEYR
383 329.7109 657.4072 657.4061 1.73 1 26 0.64 +1Score > 41 indicates identity
Score > 36 indicates homology		 IEGVIK
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