MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1501–1600 (out of 3698)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3240 682.3585 2044.0537 2044.0186 17.1 0 9 0.57 +1Score > 39 indicates identity
Score > 19 indicates homology
YYTLQILTALLMNSQNR + 3 Deamidated (NQ)
2601 562.2921 1683.8545 1683.8719 -10.4 1 9 0.51 +1Score > 40 indicates identity
Score > 19 indicates homology
FITTLADNVYSLAEK
2848 902.4133 1802.8120 1802.8179 -3.22 1 9 0.87 +1Score > 39 indicates identity
Score > 21 indicates homology
VTDMMYNLVNKAQSR + 2 Deamidated (NQ); 2 Oxidation (M)
2907 613.3381 1836.9925 1836.9833 5.01 1 9 0.89 +1Score > 37 indicates identity
Score > 21 indicates homology
QVKPDLLVVLNPTENR + 3 Deamidated (NQ)
1499 1159.7067 1158.6994 1158.7046 -4.46 1 9 0.47 +1Score > 32 indicates identity
Score > 18 indicates homology
SLPLLMITKK + Oxidation (M)
1773 647.3336 1292.6526 1292.6435 7.09 0 9 0.28 +1Score > 41 indicates identity
Score > 16 indicates homology
FVVNNMVNVLE + Oxidation (M)
2547 833.4595 1664.9044 1664.9025 1.17 0 9 0.85 +1Score > 37 indicates identity
Score > 21 indicates homology
SYDLPALLAFLSDIK
3390 709.3641 2125.0705 2125.0844 -6.56 1 9 1 +1Score > 39 indicates identity
Score > 21 indicates homology
KNLGLGNFSSGQEPFFVVGK + Deamidated (NQ)
626 389.1931 776.3716 776.3664 6.71 1 9 0.57 +1Score > 42 indicates identity
Score > 19 indicates homology
ETSSTPR
2988 471.7310 1882.8949 1882.8665 15.1 1 9 0.57 +1Score > 40 indicates identity
Score > 19 indicates homology
MATEGMILTNHDHQIR + Deamidated (NQ); Oxidation (M)
3469 1080.5978 2159.1810 2159.2064 -11.7 0 9 0.34 +1Score > 35 indicates identity
Score > 17 indicates homology
YLPLLLIIPFLAACGSSSPK
4063 892.7770 2675.3092 2675.2870 8.30 0 9 1 +1Score > 38 indicates identity
Score > 21 indicates homology
VNGHIIDDIMHDIMVKPLPQGCR + 2 Deamidated (NQ); Oxidation (M)
4829 935.4774 4672.3506 4672.3596 -1.91 1 9 0.17 +1Score > 31 indicates identity
Score > 14 indicates homology
GAVVDQLGGDLNSTPLHWAIRQGHLPMVILLLQHGADPTLTDGE + 2 Deamidated (NQ); Oxidation (M)
215 489.2799 488.2726 488.2707 3.96 0 9 0.73 +1Score > 44 indicates identity
Score > 20 indicates homology
SGGIR
554 370.7033 739.3920 739.3977 -7.61 0 9 0.72 +1Score > 38 indicates identity
Score > 20 indicates homology
AAGHGLSK
2195 752.3953 1502.7760 1502.7882 -8.06 0 9 0.9 +1Score > 41 indicates identity
Score > 21 indicates homology
YVQFLTHPGATLR + Deamidated (NQ)
2697 865.4722 1728.9298 1728.9257 2.38 0 9 0.86 +1Score > 38 indicates identity
Score > 21 indicates homology
LTANQNLIIANVTSQK + 2 Deamidated (NQ)
3601 748.4100 2242.2082 2242.1917 7.35 1 9 0.57 +1Score > 36 indicates identity
Score > 19 indicates homology
TRQQIQQLDTQLLTAISQR + 2 Deamidated (NQ)
1925 458.8864 1373.6374 1373.6575 -14.7 1 9 0.63 +1Score > 40 indicates identity
Score > 19 indicates homology
TQLWAGQENGIR + 2 Deamidated (NQ)
3118 495.9885 1979.9249 1979.9013 11.9 0 9 0.76 +1Score > 39 indicates identity
Score > 20 indicates homology
TAGYPNVNIHNFTTSWR + 3 Deamidated (NQ)
3809 795.3784 2383.1134 2383.1027 4.49 0 9 0.64 +1Score > 38 indicates identity
Score > 19 indicates homology
NISVSTQLTTNQTNPSGQISYE + 2 Deamidated (NQ)
4089 901.2006 2700.5800 2700.6306 -18.7 0 9 1 +1Score > 25 indicates identity
Score > 21 indicates homology
VLSTPPVFLLGLLLALPLGAQGLSGVR
2408 536.9327 1607.7763 1607.8011 -15.4 0 9 0.52 +1Score > 41 indicates identity
Score > 19 indicates homology
AVMLTGGMTSAANLVR + Deamidated (NQ); Oxidation (M)
2056 717.8661 1433.7176 1433.7336 -11.2 1 9 0.7 +1Score > 41 indicates identity
Score > 20 indicates homology
LQYHMQLRSIK + 2 Deamidated (NQ); Oxidation (M)
2740 876.4324 1750.8502 1750.8573 -4.03 1 9 0.84 +1Score > 41 indicates identity
Score > 21 indicates homology
ARFHDAMNILGPQHK + Deamidated (NQ); Oxidation (M)
2833 601.0164 1800.0274 1799.9993 15.6 0 9 0.44 +1Score > 33 indicates identity
Score > 18 indicates homology
ITLDVSSLAIGDSIQIR
3477 722.6876 2165.0410 2165.0497 -4.01 1 9 0.74 +1Score > 39 indicates identity
Score > 20 indicates homology
DAPVYVIACENMIGGSAILR + Deamidated (NQ); Oxidation (M)
2836 451.2079 1800.8025 1800.8200 -9.70 1 9 0.74 +1Score > 38 indicates identity
Score > 20 indicates homology
AQKNLQDHSVGNIMVE + 3 Deamidated (NQ); Oxidation (M)
3177 1006.4968 2010.9790 2010.9390 19.9 1 9 0.96 +1Score > 40 indicates identity
Score > 21 indicates homology
ISEAIMGLDAMDQAFIDR + Oxidation (M)
2672 860.4221 1718.8296 1718.7959 19.6 1 9 0.46 +1Score > 40 indicates identity
Score > 18 indicates homology
NSVVDTSRDGVDNVLE + Deamidated (NQ)
2255 510.9518 1529.8336 1529.8454 -7.70 1 9 0.78 +1Score > 39 indicates identity
Score > 20 indicates homology
GVEISTTPPIVVYR
2744 877.9588 1753.9030 1753.9223 -11.0 1 9 0.99 +1Score > 39 indicates identity
Score > 21 indicates homology
VRNNGTLDWLRPDAK
3239 681.3497 2041.0273 2040.9901 18.2 1 9 0.57 +1Score > 39 indicates identity
Score > 19 indicates homology
LGLFMPGSEVKPFMGVTSE + Oxidation (M)
1544 589.3009 1176.5872 1176.5999 -10.8 1 9 0.88 +1Score > 42 indicates identity
Score > 21 indicates homology
SPAQAYRSAAR
3176 671.3322 2010.9748 2010.9680 3.34 1 9 0.69 +1Score > 40 indicates identity
Score > 20 indicates homology
DYVASDQGKAQLSVMIDR + Oxidation (M)
1846 668.8315 1335.6484 1335.6592 -8.03 1 9 0.71 +1Score > 41 indicates identity
Score > 20 indicates homology
VVTESIGQVMQK + 2 Deamidated (NQ); Oxidation (M)
222 506.2662 505.2589 505.2570 3.77 0 9 0.54 +1Score > 44 indicates identity
Score > 19 indicates homology
MGGLL + Oxidation (M)
3246 682.6606 2044.9600 2045.0000 -19.6 0 9 0.95 +1Score > 39 indicates identity
Score > 21 indicates homology
SNPSDIVHFLTLSNNTMR
1892 455.2503 1362.7291 1362.7368 -5.64 1 9 0.64 +1Score > 40 indicates identity
Score > 19 indicates homology
VALRHTNAINPR + 2 Deamidated (NQ)
2204 752.4058 1502.7970 1502.7882 5.91 0 9 0.31 +1Score > 40 indicates identity
Score > 16 indicates homology
YVQFLTHPGATLR + Deamidated (NQ)
2931 617.2761 1848.8065 1848.7869 10.6 1 9 0.4 +1Score > 37 indicates identity
Score > 17 indicates homology
KMSGHMGAVQQSTQNLE + 4 Deamidated (NQ)
2617 565.2928 1692.8566 1692.8682 -6.89 1 9 0.93 +1Score > 40 indicates identity
Score > 21 indicates homology
QLGASALAALTTRGFSE + Deamidated (NQ)
2693 576.6642 1726.9708 1726.9505 11.7 0 9 0.51 +1Score > 35 indicates identity
Score > 18 indicates homology
ISWADLIILTGNVALE
1341 547.7850 1093.5554 1093.5768 -19.5 1 9 0.95 +1Score > 41 indicates identity
Score > 21 indicates homology
GIITYVGESR
1754 427.8983 1280.6731 1280.6500 18.0 0 9 0.59 +1Score > 39 indicates identity
Score > 19 indicates homology
VTDQISDIVYK + Deamidated (NQ)
1970 465.8961 1394.6665 1394.6466 14.2 0 9 0.68 +1Score > 41 indicates identity
Score > 20 indicates homology
WQSLLNFNSQR + 3 Deamidated (NQ)
3597 1122.1067 2242.1988 2242.1998 -0.41 1 9 0.5 +1Score > 36 indicates identity
Score > 18 indicates homology
VLPNGSLFLPAVGIQDEGIFR + Deamidated (NQ)
2738 875.4686 1748.9226 1748.8992 13.4 1 9 0.38 +1Score > 39 indicates identity
Score > 17 indicates homology
NLRADNAFMLLTQAR + Oxidation (M)
2266 512.9841 1535.9305 1535.9115 12.3 0 9 0.81 +1Score > 29 indicates identity
Score > 20 indicates homology
VFLLALIWAYISK
844 872.4576 871.4503 871.4651 -16.9 1 9 0.34 +1Score > 41 indicates identity
Score > 16 indicates homology
APVLNKTE + Deamidated (NQ)
4110 909.8254 2726.4544 2726.4023 19.1 1 9 0.19 +1Score > 35 indicates identity
Score > 14 indicates homology
FIKPLDQNMILEMAGSHDLLVTLE
2663 857.4882 1712.9618 1712.9533 4.98 1 9 0.47 +1Score > 36 indicates identity
Score > 18 indicates homology
RSGIGLLGNSIDVLSGR
2685 576.0095 1725.0067 1724.9785 16.3 1 9 0.23 +1Score > 31 indicates identity
Score > 15 indicates homology
VGLKLDQAGLDVLGTAR
2364 792.3981 1582.7816 1582.7549 16.9 0 9 0.95 +1Score > 40 indicates identity
Score > 21 indicates homology
TLNQAMFLSSVLDE + Oxidation (M)
2803 889.4735 1776.9324 1776.9040 16.0 0 9 0.73 +1Score > 39 indicates identity
Score > 20 indicates homology
DASLVAVSTGLLQNMSR + Oxidation (M)
3013 475.9765 1899.8769 1899.8924 -8.17 1 9 0.43 +1Score > 39 indicates identity
Score > 17 indicates homology
LANFDMSAFVDNAIKVE + Deamidated (NQ); Oxidation (M)
3688 767.7502 2300.2288 2300.2488 -8.72 1 9 0.26 +1Score > 36 indicates identity
Score > 15 indicates homology
GEITGSTAGLAPGHVQANLAVLPK
1113 327.1916 978.5530 978.5386 14.7 1 9 0.96 +1Score > 38 indicates identity
Score > 21 indicates homology
KIDLSQFK + Deamidated (NQ)
2300 778.8528 1555.6910 1555.7075 -10.6 1 9 0.53 +1Score > 39 indicates identity
Score > 18 indicates homology
NAILNSDFQKQMK + 4 Deamidated (NQ); Oxidation (M)
2718 578.9669 1733.8789 1733.8584 11.8 1 9 0.59 +1Score > 40 indicates identity
Score > 19 indicates homology
LNGSAVDFDVNDKALR + Deamidated (NQ)
1904 683.8592 1365.7038 1365.7292 -18.6 0 9 0.68 +1Score > 40 indicates identity
Score > 19 indicates homology
IAAQDYIIGVFR + Deamidated (NQ)
517 723.3613 722.3540 722.3520 2.75 0 9 0.41 +1Score > 41 indicates identity
Score > 17 indicates homology
TITMIE + Oxidation (M)
1719 633.8800 1265.7454 1265.7343 8.80 1 9 0.85 +1Score > 34 indicates identity
Score > 20 indicates homology
LQLQELGPVIR + Deamidated (NQ)
2097 732.3901 1462.7656 1462.7780 -8.44 1 9 0.84 +1Score > 40 indicates identity
Score > 20 indicates homology
IAETGFGTIATLGGR
3258 686.3360 2055.9862 2056.0193 -16.1 1 9 0.78 +1Score > 40 indicates identity
Score > 20 indicates homology
MAGHMGAARVTTQNLQIVR + 3 Deamidated (NQ)
3310 1043.5287 2085.0428 2085.0466 -1.79 1 9 0.79 +1Score > 39 indicates identity
Score > 20 indicates homology
YHGLSVGVILNTMHPFER + Oxidation (M)
1900 456.2300 1365.6682 1365.6565 8.57 1 9 0.82 +1Score > 41 indicates identity
Score > 20 indicates homology
VGRVFGYNNLPE + 2 Deamidated (NQ)
2998 946.4820 1890.9494 1890.9548 -2.82 1 9 0.78 +1Score > 40 indicates identity
Score > 20 indicates homology
QQGTGAVAHIQLAGESAPR + Deamidated (NQ)
1503 580.3598 1158.7050 1158.6860 16.5 0 9 0.88 +1Score > 31 indicates identity
Score > 20 indicates homology
ILDTVISSIAK
2977 469.5205 1874.0529 1874.0262 14.3 1 9 0.63 +1Score > 33 indicates identity
Score > 19 indicates homology
IRTNPTISLDLDFITR
3940 629.3148 2513.2301 2513.2696 -15.7 1 9 0.55 +1Score > 39 indicates identity
Score > 18 indicates homology
LVVSHLACADTPEHPLNATQLAR + Deamidated (NQ)
1378 557.2607 1112.5068 1112.4882 16.8 1 9 0.84 +1Score > 39 indicates identity
Score > 20 indicates homology
LEFLMGQME + Oxidation (M)
1954 694.8250 1387.6354 1387.6255 7.14 0 9 0.24 +1Score > 40 indicates identity
Score > 15 indicates homology
ADAIAIHFNSAQE + 2 Deamidated (NQ)
3112 659.0160 1974.0262 1973.9946 16.0 1 9 0.51 +1Score > 39 indicates identity
Score > 18 indicates homology
TEPITPEIVAQHGLKPDE + Deamidated (NQ)
3244 512.0315 2044.0969 2044.0589 18.6 1 9 0.63 +1Score > 37 indicates identity
Score > 19 indicates homology
SPVPTNIRHIQEPSNIIE + Deamidated (NQ)
3761 780.7188 2339.1346 2339.1514 -7.21 1 8 0.55 +1Score > 39 indicates identity
Score > 18 indicates homology
SPMLASRAGWIHTPLDVDAMR + Oxidation (M)
244 532.2825 531.2752 531.2653 18.8 1 8 0.79 +1Score > 47 indicates identity
Score > 20 indicates homology
AGKAGE
1190 509.7801 1017.5456 1017.5528 -7.07 1 8 0.26 +1Score > 41 indicates identity
Score > 15 indicates homology
KILVDGNMK + Deamidated (NQ)
1308 1078.5751 1077.5678 1077.5567 10.4 0 8 1 +1Score > 41 indicates identity
Score > 21 indicates homology
KPLQNSHPR + 2 Deamidated (NQ)
3259 686.3741 2056.1005 2056.0742 12.8 1 8 0.54 +1Score > 37 indicates identity
Score > 18 indicates homology
TGVNNAFGKNLIGLFHQPK + 2 Deamidated (NQ)
1493 579.3239 1156.6332 1156.6452 -10.3 0 8 0.88 +1Score > 40 indicates identity
Score > 20 indicates homology
QGINTIPISSK
499 357.7000 713.3854 713.3748 14.9 0 8 0.64 +1Score > 38 indicates identity
Score > 19 indicates homology
TVFYGK
855 439.2635 876.5124 876.5102 2.52 1 8 0.85 +1Score > 38 indicates identity
Score > 20 indicates homology
AMAILKSK + Oxidation (M)
1298 1074.5527 1073.5454 1073.5604 -14.0 1 8 0.42 +1Score > 42 indicates identity
Score > 17 indicates homology
QQESLNLLK + 2 Deamidated (NQ)
2198 752.3959 1502.7772 1502.7828 -3.67 1 8 0.98 +1Score > 41 indicates identity
Score > 21 indicates homology
ANLTNTSIVKALQE + 2 Deamidated (NQ)
2864 904.9907 1807.9668 1807.9502 9.20 1 8 0.6 +1Score > 38 indicates identity
Score > 19 indicates homology
VMGLDATALSYGDLVKR
3922 832.7456 2495.2150 2495.2254 -4.17 1 8 0.62 +1Score > 38 indicates identity
Score > 19 indicates homology
AGANVVFVDENIFPQTLAVMTTR + 3 Deamidated (NQ)
3005 947.5014 1892.9882 1892.9731 8.01 1 8 0.67 +1Score > 39 indicates identity
Score > 19 indicates homology
NFNVSVNILQGKISQTK + 4 Deamidated (NQ)
3987 860.0729 2577.1969 2577.1727 9.39 1 8 0.27 +1Score > 37 indicates identity
Score > 15 indicates homology
MPQGFQVVAETANAPVCAIQDTAR + 4 Deamidated (NQ)
3147 667.0024 1997.9854 1997.9953 -4.95 1 8 0.81 +1Score > 40 indicates identity
Score > 20 indicates homology
MTTAAQVSGLDVAPTRHAR + Deamidated (NQ); Oxidation (M)
2387 799.4078 1596.8010 1596.8181 -10.7 1 8 0.93 +1Score > 40 indicates identity
Score > 21 indicates homology
LDGKNMIHGIILQE + Deamidated (NQ); Oxidation (M)
2679 861.4258 1720.8370 1720.8441 -4.07 1 8 0.99 +1Score > 41 indicates identity
Score > 21 indicates homology
MELILNSLISDDLTE + Oxidation (M)
3109 986.5027 1970.9908 1970.9659 12.7 0 8 0.78 +1Score > 39 indicates identity
Score > 20 indicates homology
IGQMAQQPAAFIPDAALVE + 2 Deamidated (NQ)
4503 804.4351 3213.7113 3213.6674 13.7 1 8 0.3 +1Score > 33 indicates identity
Score > 16 indicates homology
LIIFRGLQGIGGGIMMPMAMIVIGDLFTGK + Deamidated (NQ); 4 Oxidation (M)
1985 351.9373 1403.7201 1403.7409 -14.8 0 8 0.97 +1Score > 41 indicates identity
Score > 21 indicates homology
IHTISDLLSYSR
2872 604.6699 1810.9879 1811.0152 -15.1 1 8 0.83 +1Score > 37 indicates identity
Score > 20 indicates homology
TLDRGLAILNDAVAQLK + Deamidated (NQ)
3595 747.0291 2238.0655 2238.0376 12.5 0 8 0.78 +1Score > 39 indicates identity
Score > 20 indicates homology
GHGNLMAHVDLFVPTDLDDR + Deamidated (NQ); Oxidation (M)
2523 826.4331 1650.8516 1650.8399 7.10 1 8 0.74 +1Score > 40 indicates identity
Score > 20 indicates homology
MIQAGLRIDVDTFR + Deamidated (NQ); Oxidation (M)
1293 536.3285 1070.6424 1070.6488 -5.92 1 8 0.86 +1Score > 36 indicates identity
Score > 20 indicates homology
IPFLLEALR
2768 589.0004 1763.9794 1763.9781 0.70 1 8 0.57 +1Score > 35 indicates identity
Score > 18 indicates homology
NIVHGQLITAITLDEK
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