MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1401–1500 (out of 3698)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1886 454.2667 1359.7783 1359.7762 1.54 1 10 0.67 +1Score > 35 indicates identity
Score > 20 indicates homology
TIFLPNKTQGIK + Deamidated (NQ)
2654 571.3152 1710.9238 1710.9264 -1.56 1 10 0.87 +1Score > 38 indicates identity
Score > 22 indicates homology
KGATGKPLTPDITQQR + Deamidated (NQ)
3247 1023.5359 2045.0572 2045.0694 -5.94 1 10 0.95 +1Score > 39 indicates identity
Score > 22 indicates homology
SSAGVYHYLPLGQRVLQR + 2 Deamidated (NQ)
1804 657.2753 1312.5360 1312.5419 -4.46 1 10 0.65 +1Score > 37 indicates identity
Score > 20 indicates homology
GSSEGYADVDADK
2290 775.9102 1549.8058 1549.7810 16.0 1 10 0.71 +1Score > 40 indicates identity
Score > 21 indicates homology
GGIVINAEIYVNCK + Deamidated (NQ)
3289 692.7125 2075.1157 2075.1296 -6.73 1 10 0.24 +1Score > 36 indicates identity
Score > 16 indicates homology
SVVINTLCQAQTKLGLTTK + Deamidated (NQ)
3497 725.3687 2173.0843 2173.0804 1.80 1 10 0.66 +1Score > 39 indicates identity
Score > 20 indicates homology
DKIHHVDSLHQLFSAISPE + Deamidated (NQ)
1670 618.3499 1234.6852 1234.6782 5.68 0 10 0.62 +1Score > 37 indicates identity
Score > 20 indicates homology
TGVLQTAHGPVR
2922 922.4886 1842.9626 1842.9336 15.8 1 10 0.56 +1Score > 39 indicates identity
Score > 20 indicates homology
SSNNLIWGNRLINNAR + 2 Deamidated (NQ)
1444 379.8904 1136.6494 1136.6442 4.59 0 10 0.63 +1Score > 36 indicates identity
Score > 20 indicates homology
TGTTIVGVVYK
3588 1116.5902 2231.1658 2231.1923 -11.9 1 10 0.54 +1Score > 38 indicates identity
Score > 19 indicates homology
GEPVRPALLWNDVRSAPQAR
2761 882.4688 1762.9230 1762.9505 -15.6 1 10 0.93 +1Score > 39 indicates identity
Score > 22 indicates homology
VFLPTNHEGLPVLVVE + Deamidated (NQ)
3217 679.0269 2034.0589 2034.0633 -2.18 1 10 0.94 +1Score > 38 indicates identity
Score > 22 indicates homology
LYSTSVSVPLAAIQNGAKSE
3321 1047.0332 2092.0518 2092.0245 13.1 1 10 0.22 +1Score > 39 indicates identity
Score > 16 indicates homology
DQKVLQNMADTISLIAGQK + 4 Deamidated (NQ); Oxidation (M)
3866 813.7922 2438.3548 2438.3421 5.21 1 10 0.43 +1Score > 32 indicates identity
Score > 18 indicates homology
NVVLFGHSIGGGLAIALTQVLTKE + 2 Deamidated (NQ)
2413 537.3407 1609.0003 1608.9814 11.7 1 10 0.64 +1Score > 23 indicates identity
Score > 20 indicates homology
LSPAEVALITTLLLR
1979 467.6174 1399.8304 1399.8472 -12.0 0 10 0.96 +1Score > 33 indicates identity
Score > 22 indicates homology
QVTIALLSMALIK
3245 682.3758 2044.1056 2044.0701 17.4 1 10 0.85 +1Score > 36 indicates identity
Score > 21 indicates homology
RLAQLQALIDAQQASFNR + 2 Deamidated (NQ)
2240 507.5850 1519.7332 1519.7406 -4.88 1 10 0.97 +1Score > 41 indicates identity
Score > 22 indicates homology
LANTAVSETPFLQE + Deamidated (NQ)
4261 726.3878 2901.5221 2901.5133 3.04 1 10 0.42 +1Score > 35 indicates identity
Score > 18 indicates homology
GGLQSIPKLTFPGGLLIGCSPGFMNVPK + Deamidated (NQ); Oxidation (M)
1922 457.9179 1370.7319 1370.7517 -14.5 1 10 0.63 +1Score > 40 indicates identity
Score > 20 indicates homology
GLASASIGALLENR
2675 860.4589 1718.9032 1718.8700 19.3 1 10 0.9 +1Score > 39 indicates identity
Score > 22 indicates homology
LGAGDTVSFGRGLGDAAR
2729 581.6686 1741.9840 1741.9726 6.51 1 10 0.94 +1Score > 34 indicates identity
Score > 22 indicates homology
QIQLLLQYLKNDPR + Deamidated (NQ)
3723 770.7269 2309.1589 2309.1988 -17.3 1 10 0.32 +1Score > 39 indicates identity
Score > 17 indicates homology
GFRINTNIGALNAHANSVVNAR + Deamidated (NQ)
1017 314.8369 941.4889 941.4930 -4.41 1 10 0.42 +1Score > 40 indicates identity
Score > 18 indicates homology
QKGGPALDR + Deamidated (NQ)
3582 1115.5751 2229.1356 2229.1099 11.5 1 10 0.89 +1Score > 39 indicates identity
Score > 22 indicates homology
VTDPNNLIMNVNPLRLNYE + Deamidated (NQ)
3304 1041.5090 2081.0034 2081.0364 -15.8 1 10 0.36 +1Score > 40 indicates identity
Score > 18 indicates homology
VSLFGALVHSRTYDMDVR + Oxidation (M)
2913 920.4438 1838.8730 1838.8832 -5.54 1 10 0.82 +1Score > 40 indicates identity
Score > 21 indicates homology
TLGSRYVLQGNMEPTR + 2 Deamidated (NQ); Oxidation (M)
2041 713.9284 1425.8422 1425.8191 16.2 0 10 0.65 +1Score > 33 indicates identity
Score > 20 indicates homology
LLIGDAVNLQTLR + Deamidated (NQ)
2957 622.6657 1864.9753 1864.9604 7.95 0 9 0.95 +1Score > 39 indicates identity
Score > 22 indicates homology
QLFQLLSDSDVNVLMK + Oxidation (M)
1650 410.5760 1228.7062 1228.7139 -6.32 1 9 0.88 +1Score > 38 indicates identity
Score > 21 indicates homology
QTQVTVQKIGK
2417 806.4319 1610.8492 1610.8412 5.01 1 9 0.72 +1Score > 39 indicates identity
Score > 21 indicates homology
KMVLQVIDIMFNK + Deamidated (NQ); 2 Oxidation (M)
2625 566.2904 1695.8494 1695.8726 -13.7 1 9 0.66 +1Score > 40 indicates identity
Score > 20 indicates homology
AVCGKPKNPVDQVQR + Deamidated (NQ)
3040 639.3276 1914.9610 1914.9727 -6.13 1 9 0.21 +1Score > 40 indicates identity
Score > 15 indicates homology
FNILGYVRPELDYTSK + Deamidated (NQ)
3162 1004.5801 2007.1456 2007.1405 2.59 1 9 0.26 +1Score > 31 indicates identity
Score > 16 indicates homology
IVLGIDPALGSLGWAVVAKE
2485 546.3295 1635.9667 1635.9559 6.57 1 9 0.91 +1Score > 30 indicates identity
Score > 22 indicates homology
ANLKLLLVGIGNPGQK + 2 Deamidated (NQ)
3328 699.3728 2095.0966 2095.1367 -19.2 1 9 0.59 +1Score > 38 indicates identity
Score > 20 indicates homology
LIGQRFWHTAVLLVQWE
2196 752.3954 1502.7762 1502.7828 -4.33 1 9 0.97 +1Score > 41 indicates identity
Score > 22 indicates homology
ANLTNTSIVKALQE + 2 Deamidated (NQ)
2876 907.0084 1812.0022 1812.0356 -18.4 1 9 0.75 +1Score > 36 indicates identity
Score > 21 indicates homology
SLGINLDLLLLRTNQK + 2 Deamidated (NQ)
1034 947.5005 946.4932 946.4906 2.77 1 9 0.61 +1Score > 42 indicates identity
Score > 20 indicates homology
GRGLMVGIE + Oxidation (M)
2192 501.9298 1502.7676 1502.7399 18.4 0 9 0.75 +1Score > 41 indicates identity
Score > 21 indicates homology
NPSSDLAMGLNVIR + Deamidated (NQ); Oxidation (M)
2047 715.8737 1429.7328 1429.7412 -5.85 1 9 0.88 +1Score > 41 indicates identity
Score > 21 indicates homology
ANSINSKLNILNE + Deamidated (NQ)
2939 928.4562 1854.8978 1854.9072 -5.01 0 9 0.72 +1Score > 40 indicates identity
Score > 21 indicates homology
TIDNTGKPTNVQANPQR + 2 Deamidated (NQ)
2528 551.9501 1652.8285 1652.8152 8.04 1 9 0.41 +1Score > 40 indicates identity
Score > 18 indicates homology
AGQKIATMGSTGTSSTR
4424 784.3951 3133.5513 3133.5028 15.5 1 9 0.19 +1Score > 36 indicates identity
Score > 15 indicates homology
FEVVLYLNQMGVFNFVDDGSLPGCAVLK + 3 Deamidated (NQ)
2267 769.3880 1536.7614 1536.7532 5.35 1 9 0.84 +1Score > 41 indicates identity
Score > 21 indicates homology
VPNKNHGSVSQLQE + Deamidated (NQ)
2470 816.4521 1630.8896 1630.8719 10.9 0 9 0.9 +1Score > 38 indicates identity
Score > 21 indicates homology
LTLHGLQQYFVALE
2612 423.7294 1690.8885 1690.9123 -14.1 1 9 0.51 +1Score > 39 indicates identity
Score > 19 indicates homology
HLRMMHLAATLIQR + Deamidated (NQ)
3561 737.3892 2209.1458 2209.1729 -12.3 1 9 0.47 +1Score > 38 indicates identity
Score > 19 indicates homology
LLQIEDDNGGILAIPAVDTIK + 2 Deamidated (NQ)
3653 1140.0873 2278.1600 2278.1184 18.3 1 9 0.62 +1Score > 39 indicates identity
Score > 20 indicates homology
ASSIANVMIAAILRNDMTLAGE + 2 Deamidated (NQ); Oxidation (M)
2369 397.4733 1585.8641 1585.8464 11.2 0 9 0.63 +1Score > 38 indicates identity
Score > 20 indicates homology
VVVAYGGAPINQQLR + 2 Deamidated (NQ)
2544 831.9503 1661.8860 1661.8923 -3.75 1 9 0.23 +1Score > 39 indicates identity
Score > 16 indicates homology
SRSLCNIPFITINK
3978 855.1285 2562.3637 2562.3138 19.5 1 9 1 +1Score > 35 indicates identity
Score > 22 indicates homology
NLPTAMQLSIALDINFSIIDKVE + 2 Deamidated (NQ); Oxidation (M)
3495 1087.5427 2173.0708 2173.0460 11.4 1 9 0.19 +1Score > 39 indicates identity
Score > 15 indicates homology
LNMDGNNLVTIPSELPSTLE + Deamidated (NQ); Oxidation (M)
3950 631.8021 2523.1793 2523.1960 -6.63 1 9 0.37 +1Score > 37 indicates identity
Score > 17 indicates homology
NLDSFMLPPPVLNALFCEQMR + 2 Oxidation (M)
1998 704.3603 1406.7060 1406.7075 -1.02 1 9 0.37 +1Score > 41 indicates identity
Score > 17 indicates homology
MSQKQTKPAAATK + 2 Deamidated (NQ); Oxidation (M)
2721 869.9695 1737.9244 1737.9045 11.5 1 9 0.21 +1Score > 38 indicates identity
Score > 15 indicates homology
MMSLGIQFILLGTGEK + Deamidated (NQ)
1507 387.8816 1160.6230 1160.6376 -12.6 1 9 0.78 +1Score > 41 indicates identity
Score > 21 indicates homology
NVPMGFKLVR + Deamidated (NQ)
2008 706.9087 1411.8028 1411.7823 14.5 0 9 0.66 +1Score > 37 indicates identity
Score > 20 indicates homology
WIGDQLLLNLAR + Deamidated (NQ)
2770 883.9402 1765.8658 1765.8622 2.09 1 9 0.91 +1Score > 40 indicates identity
Score > 21 indicates homology
IEAIANSVLDGITGYTE + Deamidated (NQ)
1338 365.2096 1092.6070 1092.5928 13.0 1 9 0.92 +1Score > 37 indicates identity
Score > 21 indicates homology
RVGAVDVSYK
3867 814.3860 2440.1362 2440.1336 1.07 1 9 0.8 +1Score > 38 indicates identity
Score > 21 indicates homology
RAVRPFSDVFYSDFYADGVTE
2108 735.3729 1468.7312 1468.7521 -14.2 0 9 0.85 +1Score > 40 indicates identity
Score > 21 indicates homology
QIQAVPIANSQAAR + 3 Deamidated (NQ)
2767 588.9937 1763.9593 1763.9491 5.74 1 9 0.76 +1Score > 37 indicates identity
Score > 21 indicates homology
YNVIVKGINGTPMTIK + Deamidated (NQ); Oxidation (M)
1281 356.5375 1066.5907 1066.5811 8.95 1 9 0.74 +1Score > 37 indicates identity
Score > 20 indicates homology
DNGIKFFVK
2882 909.4986 1816.9826 1816.9571 14.1 1 9 0.42 +1Score > 38 indicates identity
Score > 18 indicates homology
DKVLIVDDFLANGQAAK + Deamidated (NQ)
1502 1159.7113 1158.7040 1158.7046 -0.49 1 9 0.74 +1Score > 31 indicates identity
Score > 20 indicates homology
SLPLLMITKK + Oxidation (M)
2435 538.9540 1613.8402 1613.8373 1.81 1 9 0.58 +1Score > 40 indicates identity
Score > 19 indicates homology
LNRQNSQLQLIASE + Deamidated (NQ)
2849 601.9822 1802.9248 1802.8907 18.9 0 9 0.8 +1Score > 40 indicates identity
Score > 21 indicates homology
DGAVVMMGATINIGAVVGE
2936 928.4478 1854.8810 1854.8748 3.38 0 9 0.75 +1Score > 40 indicates identity
Score > 20 indicates homology
EPSGGNNWAPLQAGDTLK + Deamidated (NQ)
3041 959.0056 1915.9966 1915.9673 15.3 1 9 0.41 +1Score > 39 indicates identity
Score > 18 indicates homology
VSIGSQELQIVCGANNVK + Deamidated (NQ)
3670 765.3914 2293.1524 2293.1127 17.3 1 9 0.59 +1Score > 39 indicates identity
Score > 19 indicates homology
ALRYYSPPPTNGQSPNFDLR + Deamidated (NQ)
2035 475.5767 1423.7083 1423.7195 -7.86 0 9 0.83 +1Score > 41 indicates identity
Score > 21 indicates homology
YATADLSVQTDIK
2247 764.4435 1526.8724 1526.8529 12.8 1 9 0.99 +1Score > 35 indicates identity
Score > 22 indicates homology
AVAAAPAGTTSSRVLR
2488 546.9703 1637.8891 1637.8889 0.10 1 9 0.71 +1Score > 37 indicates identity
Score > 20 indicates homology
INRTANLFYSLGIR + Deamidated (NQ)
651 395.2049 788.3952 788.4028 -9.56 0 9 0.92 +1Score > 44 indicates identity
Score > 21 indicates homology
ISINANR + 2 Deamidated (NQ)
1018 942.5053 941.4980 941.5004 -2.55 1 9 0.8 +1Score > 39 indicates identity
Score > 21 indicates homology
VLEVMAHK + Oxidation (M)
3934 628.3214 2509.2565 2509.2384 7.23 1 9 0.42 +1Score > 38 indicates identity
Score > 18 indicates homology
IAFVSNRGGSPQVYIMNADGTGVR + Deamidated (NQ)
3489 724.7304 2171.1694 2171.2062 -17.0 1 9 0.61 +1Score > 36 indicates identity
Score > 20 indicates homology
SGFRPLRATGSALNIASLVLE
3788 788.7257 2363.1553 2363.1977 -18.0 1 9 0.64 +1Score > 39 indicates identity
Score > 20 indicates homology
IVFLGAGSAGCGIAEMIIAQTQR + Deamidated (NQ)
2555 834.4700 1666.9254 1666.9366 -6.68 1 9 0.45 +1Score > 36 indicates identity
Score > 18 indicates homology
LQPLLQSTIIRNDR + Deamidated (NQ)
2680 861.4655 1720.9164 1720.8856 17.9 1 9 0.41 +1Score > 38 indicates identity
Score > 18 indicates homology
ATVEGGQHLNVNVLNR + Deamidated (NQ)
3066 646.3643 1936.0711 1936.1074 -18.8 0 9 0.2 +1Score > 35 indicates identity
Score > 15 indicates homology
QFLASLVSFLLVVWSVK + Deamidated (NQ)
3599 1122.1073 2242.2000 2242.1766 10.5 1 9 0.47 +1Score > 36 indicates identity
Score > 18 indicates homology
SVSKIIANGINIALQPNMVLE + 3 Deamidated (NQ); Oxidation (M)
1889 455.2305 1362.6697 1362.6891 -14.3 1 9 0.49 +1Score > 41 indicates identity
Score > 19 indicates homology
ILQQADNDYKR
2106 735.3696 1468.7246 1468.7344 -6.62 1 9 0.67 +1Score > 40 indicates identity
Score > 20 indicates homology
NETIMNIIDHIR + Deamidated (NQ)
2930 617.2750 1848.8032 1848.7869 8.78 1 9 0.2 +1Score > 37 indicates identity
Score > 15 indicates homology
KMSGHMGAVQQSTQNLE + 4 Deamidated (NQ)
3184 1010.0072 2017.9998 2018.0216 -10.8 1 9 0.97 +1Score > 40 indicates identity
Score > 22 indicates homology
LESIVMNWLGQMLNLPK + Deamidated (NQ); 2 Oxidation (M)
232 518.2979 517.2906 517.2972 -12.8 1 9 0.73 +1Score > 45 indicates identity
Score > 20 indicates homology
RTGGK
2928 924.5013 1846.9880 1846.9611 14.6 1 9 0.63 +1Score > 38 indicates identity
Score > 20 indicates homology
AGATGFCVAILDEALALR
968 463.2259 924.4372 924.4454 -8.79 0 9 0.82 +1Score > 41 indicates identity
Score > 21 indicates homology
GNGFLFDR
3680 574.7944 2295.1485 2295.1647 -7.08 1 9 0.82 +1Score > 39 indicates identity
Score > 21 indicates homology
LLQHFGSLHGLLNANLDEFR + 2 Deamidated (NQ)
3285 692.3809 2074.1209 2074.1324 -5.54 1 9 0.24 +1Score > 36 indicates identity
Score > 15 indicates homology
QITPKPIGVGFGIASPEHAR
3681 1149.1410 2296.2674 2296.2249 18.5 1 9 0.32 +1Score > 34 indicates identity
Score > 17 indicates homology
AAEIVFQSGIPVVMAGLNVTHK + Oxidation (M)
2434 807.9266 1613.8386 1613.8334 3.25 1 9 0.73 +1Score > 40 indicates identity
Score > 20 indicates homology
KLNINLAMSPLPSSE + Deamidated (NQ)
3504 726.6904 2177.0494 2177.0310 8.45 1 9 0.3 +1Score > 39 indicates identity
Score > 16 indicates homology
YLQALGNPAMQNLAADELAR + 3 Deamidated (NQ); Oxidation (M)
2503 823.4295 1644.8444 1644.8570 -7.62 0 9 0.87 +1Score > 41 indicates identity
Score > 21 indicates homology
IIGNQNQNSLLSLTK + 3 Deamidated (NQ)
4488 1068.5480 3202.6222 3202.5969 7.90 1 9 1 +1Score > 36 indicates identity
Score > 22 indicates homology
DIAGQAQTGTGKTMAFLVATFNHLLNVAAPE + Deamidated (NQ); Oxidation (M)
1437 378.8712 1133.5918 1133.5968 -4.48 1 9 0.52 +1Score > 42 indicates identity
Score > 19 indicates homology
EGIVIAVGTFE
511 361.2166 720.4186 720.4204 -2.40 1 9 0.97 +1Score > 38 indicates identity
Score > 21 indicates homology
MSKIVK + Oxidation (M)
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