MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1201–1300 (out of 3698)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1547 393.8888 1178.6446 1178.6403 3.63 1 11 0.64 +1Score > 38 indicates identity
Score > 22 indicates homology
ALLDVMKMVK + 2 Oxidation (M)
1573 596.3004 1190.5862 1190.5965 -8.62 1 11 0.65 +1Score > 42 indicates identity
Score > 22 indicates homology
GQIDIELTMR + Oxidation (M)
3539 550.2756 2197.0733 2197.0386 15.8 1 11 0.77 +1Score > 39 indicates identity
Score > 23 indicates homology
SSRNASISNGTSISDTIFPIE + 2 Deamidated (NQ)
2376 530.9055 1589.6947 1589.7007 -3.76 0 11 0.4 +1Score > 38 indicates identity
Score > 20 indicates homology
DHGMYFMYQILR + Deamidated (NQ); Oxidation (M)
3167 670.7043 2009.0911 2009.0582 16.4 1 11 0.25 +1Score > 36 indicates identity
Score > 18 indicates homology
QKQTLLNIGFGNFVVSSR + 2 Deamidated (NQ)
2546 555.2968 1662.8686 1662.8940 -15.3 1 11 0.88 +1Score > 40 indicates identity
Score > 23 indicates homology
LAQKYNTISQLLDR + Deamidated (NQ)
848 437.7168 873.4190 873.4344 -17.6 1 11 0.54 +1Score > 41 indicates identity
Score > 21 indicates homology
LKHNFSE
3200 1012.5261 2023.0376 2023.0698 -15.9 1 11 0.73 +1Score > 39 indicates identity
Score > 22 indicates homology
SAALQSIQENLGLQDAPLR
3616 750.7111 2249.1115 2249.1249 -5.97 0 11 0.94 +1Score > 39 indicates identity
Score > 23 indicates homology
QVGLIQLMAQIGSYVPAQSAR + 4 Deamidated (NQ); Oxidation (M)
3392 709.3860 2125.1362 2125.1313 2.27 1 11 0.54 +1Score > 37 indicates identity
Score > 21 indicates homology
RNLGDLCGLQVLLIGAGGAAR + 2 Deamidated (NQ)
2027 474.9420 1421.8042 1421.8031 0.78 1 11 0.67 +1Score > 36 indicates identity
Score > 22 indicates homology
IRNFFIAVLSDK
3711 1154.0541 2306.0936 2306.0962 -1.12 1 11 0.45 +1Score > 39 indicates identity
Score > 20 indicates homology
MNNLIKAYAAGVMSAAFLFGSE + 2 Deamidated (NQ)
886 446.7254 891.4362 891.4371 -1.01 0 11 0.53 +1Score > 42 indicates identity
Score > 21 indicates homology
NLMATPTK + Deamidated (NQ); Oxidation (M)
2418 806.4360 1610.8574 1610.8450 7.73 1 11 0.35 +1Score > 39 indicates identity
Score > 19 indicates homology
QMKHLVQALQASLE + Oxidation (M)
1860 448.9033 1343.6881 1343.6793 6.50 1 11 0.87 +1Score > 41 indicates identity
Score > 23 indicates homology
GRDLITADDVNR
1992 703.8934 1405.7722 1405.7790 -4.78 1 11 0.83 +1Score > 38 indicates identity
Score > 23 indicates homology
APARGAAPVNNLQK
2521 825.9329 1649.8512 1649.8624 -6.78 0 11 0.46 +1Score > 40 indicates identity
Score > 20 indicates homology
ITTVQAAIDFIQSSR + Deamidated (NQ)
2315 782.3931 1562.7716 1562.7664 3.39 1 11 0.82 +1Score > 41 indicates identity
Score > 23 indicates homology
RMNYNQILFHVE
2790 590.9610 1769.8612 1769.8804 -10.9 1 11 0.67 +1Score > 40 indicates identity
Score > 22 indicates homology
HIMLVADVMTADGELR
2950 620.9929 1859.9569 1859.9736 -9.02 1 11 0.34 +1Score > 40 indicates identity
Score > 19 indicates homology
IVLQPDETIQISMMVK + Oxidation (M)
2190 752.3901 1502.7656 1502.7828 -11.4 1 11 0.71 +1Score > 41 indicates identity
Score > 22 indicates homology
ANLTNTSIVKALQE + 2 Deamidated (NQ)
3409 1068.0963 2134.1780 2134.1899 -5.53 1 11 0.38 +1Score > 34 indicates identity
Score > 19 indicates homology
LDSPYGIRLIQGSHLIVPR + Deamidated (NQ)
1924 686.8364 1371.6582 1371.6684 -7.39 1 11 1 +1Score > 40 indicates identity
Score > 24 indicates homology
KGPGNFSSVHWR + Deamidated (NQ)
2743 877.4625 1752.9104 1752.9046 3.34 1 11 0.53 +1Score > 39 indicates identity
Score > 21 indicates homology
ILNPSLDNLGYRYSK + Deamidated (NQ)
3197 1012.5245 2023.0344 2023.0698 -17.5 1 11 0.49 +1Score > 39 indicates identity
Score > 21 indicates homology
SAALQSIQENLGLQDAPLR
1322 362.5459 1084.6159 1084.6281 -11.2 0 11 0.23 +1Score > 40 indicates identity
Score > 17 indicates homology
SPVKPGIPYK
2812 894.4796 1786.9446 1786.9676 -12.9 1 11 0.74 +1Score > 39 indicates identity
Score > 22 indicates homology
IQALTTVNQKVGTNIGK + 3 Deamidated (NQ)
3394 709.7190 2126.1352 2126.0983 17.4 1 11 0.38 +1Score > 37 indicates identity
Score > 19 indicates homology
VHPNSIHICAVLVEYFTK
1797 437.2225 1308.6457 1308.6271 14.2 0 11 0.67 +1Score > 42 indicates identity
Score > 22 indicates homology
MNLTLNFVDPK + 2 Deamidated (NQ); Oxidation (M)
2660 429.2141 1712.8273 1712.8104 9.85 0 11 0.81 +1Score > 40 indicates identity
Score > 23 indicates homology
NQSLSLQNDQEPPLK + 3 Deamidated (NQ)
2819 597.3120 1788.9142 1788.9444 -16.9 1 11 0.6 +1Score > 40 indicates identity
Score > 21 indicates homology
AQVQHMVHLLLQLEK + 3 Deamidated (NQ)
3942 840.4327 2518.2763 2518.2904 -5.59 1 11 0.13 +1Score > 38 indicates identity
Score > 15 indicates homology
SGIPVMGHIGLTPQFVNRFGGYR + Oxidation (M)
2925 923.4431 1844.8716 1844.8527 10.3 1 11 0.45 +1Score > 40 indicates identity
Score > 20 indicates homology
ADIAVGEQVQVVNVTNGE + 4 Deamidated (NQ)
2961 933.9272 1865.8398 1865.8499 -5.39 0 11 0.65 +1Score > 39 indicates identity
Score > 22 indicates homology
LDLMDQGTNAMDILSGR + Deamidated (NQ); Oxidation (M)
1553 591.3651 1180.7156 1180.6928 19.4 1 11 0.26 +1Score > 32 indicates identity
Score > 18 indicates homology
QLLVPADIRR + Deamidated (NQ)
3536 733.3632 2197.0678 2197.0434 11.1 0 11 0.52 +1Score > 39 indicates identity
Score > 21 indicates homology
DHTKPVQQVLADMTNGGVDR + Deamidated (NQ); Oxidation (M)
1525 391.2064 1170.5974 1170.6132 -13.5 0 11 0.93 +1Score > 40 indicates identity
Score > 23 indicates homology
QAAQAVIDLLE + Deamidated (NQ)
1562 593.3485 1184.6824 1184.6951 -10.7 1 11 0.84 +1Score > 38 indicates identity
Score > 23 indicates homology
VVKLCPTGAIK
4143 922.8176 2765.4310 2765.4383 -2.66 1 11 0.36 +1Score > 36 indicates identity
Score > 19 indicates homology
WALYVCPAVLLATQMSLAASLETLK + Deamidated (NQ); Oxidation (M)
3537 733.3645 2197.0717 2197.0434 12.9 0 11 0.42 +1Score > 39 indicates identity
Score > 20 indicates homology
DHTKPVQQVLADMTNGGVDR + Deamidated (NQ); Oxidation (M)
3712 1154.0543 2306.0940 2306.0962 -0.95 1 11 0.35 +1Score > 39 indicates identity
Score > 19 indicates homology
MNNLIKAYAAGVMSAAFLFGSE + 2 Deamidated (NQ)
3813 796.0902 2385.2488 2385.2613 -5.26 1 11 0.41 +1Score > 37 indicates identity
Score > 20 indicates homology
LAQVAVNMDQTLAAWLAKKPSK + 3 Deamidated (NQ)
2099 489.2620 1464.7642 1464.7572 4.73 1 11 0.94 +1Score > 40 indicates identity
Score > 23 indicates homology
SRILGQGGQGTVYK + 2 Deamidated (NQ)
2202 752.4020 1502.7894 1502.8093 -13.2 0 11 0.92 +1Score > 40 indicates identity
Score > 23 indicates homology
LNAVQILYQAQNK + Deamidated (NQ)
2727 581.6653 1741.9741 1741.9475 15.2 1 11 0.54 +1Score > 36 indicates identity
Score > 21 indicates homology
QVVLGGTSKYLINGHR + Deamidated (NQ)
486 354.1785 706.3424 706.3497 -10.3 1 11 0.66 +1Score > 41 indicates identity
Score > 22 indicates homology
TTDKDK
2784 590.2921 1767.8545 1767.8832 -16.2 0 11 0.58 +1Score > 40 indicates identity
Score > 21 indicates homology
GPNFYVPFSNITGVVR + 2 Deamidated (NQ)
1642 614.3717 1226.7288 1226.7347 -4.73 1 11 0.92 +1Score > 36 indicates identity
Score > 23 indicates homology
KVIQNVLSVAR + Deamidated (NQ)
2092 730.4321 1458.8496 1458.8235 18.0 0 11 0.48 +1Score > 33 indicates identity
Score > 20 indicates homology
IQWLLAYVLSPR + Deamidated (NQ)
2201 752.3977 1502.7808 1502.7980 -11.4 1 11 0.88 +1Score > 41 indicates identity
Score > 23 indicates homology
LTYLPTLSQENPK
2752 880.4494 1758.8842 1758.8682 9.10 1 11 0.77 +1Score > 40 indicates identity
Score > 22 indicates homology
QLQLAMHNSKAASSTR + Deamidated (NQ); Oxidation (M)
3262 687.3538 2059.0396 2059.0633 -11.5 1 11 0.82 +1Score > 39 indicates identity
Score > 23 indicates homology
MAGGGQVVLRTLSQQGWVR + Deamidated (NQ); Oxidation (M)
634 390.2020 778.3894 778.3861 4.31 1 11 0.81 +1Score > 43 indicates identity
Score > 23 indicates homology
LGANFKE + Deamidated (NQ)
3479 722.6893 2165.0461 2165.0833 -17.2 1 11 0.16 +1Score > 39 indicates identity
Score > 15 indicates homology
GLVKDFADLYAVPFPAWQE
2469 544.6305 1630.8697 1630.8825 -7.84 1 11 0.37 +1Score > 39 indicates identity
Score > 19 indicates homology
SIISAGCLIRGQVTR + Deamidated (NQ)
546 368.2244 734.4342 734.4327 2.14 0 11 0.93 +1Score > 36 indicates identity
Score > 23 indicates homology
IGGIFTK
3353 1056.0454 2110.0762 2110.1133 -17.5 1 11 0.74 +1Score > 39 indicates identity
Score > 22 indicates homology
AGECPYQVSLPPLTIQLPK
1732 425.5518 1273.6336 1273.6085 19.7 0 11 0.71 +1Score > 41 indicates identity
Score > 22 indicates homology
TGSSLAPQNMPR + Oxidation (M)
3293 693.3727 2077.0963 2077.0739 10.8 1 11 0.46 +1Score > 38 indicates identity
Score > 20 indicates homology
QGGQLMILGSGAPHLEQGIR + Oxidation (M)
406 336.2270 670.4394 670.4377 2.54 0 11 0.99 +1Score > 37 indicates identity
Score > 23 indicates homology
AGALVLK
956 920.4532 919.4459 919.4321 15.1 0 11 0.13 +1Score > 42 indicates identity
Score > 14 indicates homology
MNILINGE + Deamidated (NQ); Oxidation (M)
1316 542.3246 1082.6346 1082.6335 1.03 1 11 0.17 +1Score > 36 indicates identity
Score > 16 indicates homology
EPLILRTIE
1934 690.3362 1378.6578 1378.6438 10.2 1 11 0.78 +1Score > 41 indicates identity
Score > 22 indicates homology
EPYENMGAQLVK + Deamidated (NQ)
2588 837.4651 1672.9156 1672.9069 5.21 1 11 0.47 +1Score > 37 indicates identity
Score > 20 indicates homology
KLVILGGAMQGNVLDK + 2 Deamidated (NQ); Oxidation (M)
3492 544.2697 2173.0497 2173.0871 -17.2 0 11 0.87 +1Score > 39 indicates identity
Score > 23 indicates homology
SMCTPLPVAAQSTTLPSLSGR
2695 864.9667 1727.9188 1727.9053 7.83 1 11 0.52 +1Score > 39 indicates identity
Score > 21 indicates homology
QIKLQQQSNAQLVAR + 4 Deamidated (NQ)
3839 803.0901 2406.2485 2406.2101 15.9 0 11 0.35 +1Score > 37 indicates identity
Score > 19 indicates homology
MTSGQVFVDLGSGVGNVVLQAALE + Oxidation (M)
1987 351.9377 1403.7217 1403.7310 -6.60 1 11 0.77 +1Score > 41 indicates identity
Score > 22 indicates homology
AGPRPEGYFAALR
3303 1041.0509 2080.0872 2080.1164 -14.0 1 11 0.85 +1Score > 38 indicates identity
Score > 23 indicates homology
QDNLIVAIVDNQSPNAKLK + Deamidated (NQ)
3488 724.7262 2171.1568 2171.1474 4.34 1 11 0.94 +1Score > 37 indicates identity
Score > 23 indicates homology
TLYQRLGINASPIQLPIGSE + 2 Deamidated (NQ)
2549 417.4842 1665.9077 1665.9090 -0.78 1 11 0.59 +1Score > 37 indicates identity
Score > 21 indicates homology
IFIGQQGSSPKTLYK
3030 478.7525 1910.9809 1910.9698 5.82 1 11 0.61 +1Score > 39 indicates identity
Score > 21 indicates homology
VTVPNNVRVQNVDLTNE + Deamidated (NQ)
355 637.3109 636.3036 636.3153 -18.3 0 11 0.24 +1Score > 41 indicates identity
Score > 17 indicates homology
TVTIGM + Oxidation (M)
2906 919.5018 1836.9890 1836.9806 4.60 1 11 0.53 +1Score > 37 indicates identity
Score > 20 indicates homology
SSPPNAQVTVTPSGRALR
2935 618.9712 1853.8918 1853.8604 16.9 1 11 0.85 +1Score > 40 indicates identity
Score > 23 indicates homology
QLMSDDISEGIIQFLE + Deamidated (NQ); Oxidation (M)
3195 1012.5212 2023.0278 2023.0585 -15.2 1 11 0.4 +1Score > 39 indicates identity
Score > 19 indicates homology
AISALNNVIPEGGSQPLSVR + 2 Deamidated (NQ)
202 474.2695 473.2622 473.2598 5.16 0 11 0.82 +1Score > 47 indicates identity
Score > 22 indicates homology
AAAGGK
2096 366.1926 1460.7413 1460.7372 2.82 1 11 0.78 +1Score > 41 indicates identity
Score > 22 indicates homology
RQNDIPLFDTSR
3135 996.0193 1990.0240 1990.0081 8.02 1 11 0.48 +1Score > 39 indicates identity
Score > 20 indicates homology
QTTEPNYPEMLLIAINK + Oxidation (M)
3050 641.9767 1922.9083 1922.9309 -11.8 1 11 0.83 +1Score > 40 indicates identity
Score > 22 indicates homology
MNRLGFVFQSAPHSTSK + Deamidated (NQ); Oxidation (M)
3621 564.5301 2254.0913 2254.0729 8.18 1 11 0.85 +1Score > 39 indicates identity
Score > 22 indicates homology
FTGDPAHAVNFMTFIATELR + Deamidated (NQ); Oxidation (M)
2706 866.9664 1731.9182 1731.9155 1.56 1 11 0.95 +1Score > 39 indicates identity
Score > 23 indicates homology
LSPTIDVNDFNTKLR
2805 593.3193 1776.9361 1776.9509 -8.34 0 11 0.36 +1Score > 39 indicates identity
Score > 19 indicates homology
AISLAYATGQSLLGLIGE + Deamidated (NQ)
3645 756.3853 2266.1341 2266.1668 -14.4 0 11 0.3 +1Score > 39 indicates identity
Score > 18 indicates homology
LFQIGVAPSSLTLVAVTHMHE + Deamidated (NQ); Oxidation (M)
3055 642.9924 1925.9554 1925.9694 -7.28 1 11 0.66 +1Score > 39 indicates identity
Score > 21 indicates homology
TAANAEVDQAPNLAAVTAAK + Deamidated (NQ)
2866 604.2859 1809.8359 1809.8423 -3.55 0 11 0.95 +1Score > 39 indicates identity
Score > 23 indicates homology
AMLTLMLQAMGQADAGR + Deamidated (NQ); 2 Oxidation (M)
3910 830.7617 2489.2633 2489.2274 14.4 1 11 0.31 +1Score > 38 indicates identity
Score > 18 indicates homology
GFSIDLWAMPVQRPYNPNARR + 2 Deamidated (NQ)
671 399.2114 796.4082 796.4153 -8.83 0 11 0.54 +1Score > 39 indicates identity
Score > 20 indicates homology
MNLLYK + Oxidation (M)
3036 638.9891 1913.9455 1913.9703 -13.0 0 11 0.81 +1Score > 40 indicates identity
Score > 22 indicates homology
QALGVGVAQCVAMIPGVSR + 2 Deamidated (NQ)
1731 637.8124 1273.6102 1273.5972 10.2 1 11 0.34 +1Score > 41 indicates identity
Score > 18 indicates homology
DHSGALSALMEK + Oxidation (M)
3458 1077.1168 2152.2190 2152.2038 7.08 1 11 0.52 +1Score > 32 indicates identity
Score > 20 indicates homology
LPGDISGVKPLGLMDITVRR + Oxidation (M)
3402 711.6915 2132.0527 2132.0354 8.11 0 11 0.54 +1Score > 39 indicates identity
Score > 20 indicates homology
YFPDPDLPPYPITQDLIE
3029 637.6671 1909.9795 1910.0109 -16.5 1 11 0.87 +1Score > 39 indicates identity
Score > 22 indicates homology
LSVPKNVQVQNIDLTNE
2189 752.3901 1502.7656 1502.7477 11.9 1 11 0.92 +1Score > 41 indicates identity
Score > 23 indicates homology
NSIHSIYTELNGR
1538 587.3270 1172.6394 1172.6288 9.04 1 11 0.7 +1Score > 41 indicates identity
Score > 21 indicates homology
VADSALKILDE
1893 455.5554 1363.6444 1363.6176 19.6 0 11 0.83 +1Score > 41 indicates identity
Score > 22 indicates homology
MLANQANLIDNK + 4 Deamidated (NQ); Oxidation (M)
1429 378.1978 1131.5716 1131.5673 3.81 0 10 0.47 +1Score > 42 indicates identity
Score > 20 indicates homology
AATNPSTAFPR
2289 517.2710 1548.7912 1548.7645 17.2 1 10 0.74 +1Score > 41 indicates identity
Score > 22 indicates homology
QGYNVRISGQDVGR + Deamidated (NQ)
2834 451.2072 1800.7997 1800.7698 16.6 0 10 0.83 +1Score > 38 indicates identity
Score > 22 indicates homology
NDPTLMFANAGMNQFK + 3 Deamidated (NQ)
1751 427.5927 1279.7563 1279.7798 -18.4 1 10 0.37 +1Score > 34 indicates identity
Score > 19 indicates homology
LGRKPIIVCPK
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