MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1001–1100 (out of 3698)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
369 323.7005 645.3864 645.3850 2.28 0 13 0.99 +1Score > 39 indicates identity
Score > 25 indicates homology
FAPAIK
1447 570.2629 1138.5112 1138.5117 -0.43 1 13 0.24 +1Score > 40 indicates identity
Score > 19 indicates homology
FSAMGVWNEV
2175 500.2917 1497.8533 1497.8667 -8.98 1 13 0.34 +1Score > 36 indicates identity
Score > 21 indicates homology
RVFQAAQALGIPVK + Deamidated (NQ)
2309 780.9390 1559.8634 1559.8527 6.89 1 13 0.43 +1Score > 37 indicates identity
Score > 22 indicates homology
KSLCANILMIKPR + Deamidated (NQ); Oxidation (M)
2023 474.8921 1421.6545 1421.6344 14.1 1 13 0.67 +1Score > 41 indicates identity
Score > 24 indicates homology
DMEQALNTIQNK + 2 Deamidated (NQ); Oxidation (M)
431 342.2271 682.4396 682.4378 2.77 0 13 0.33 +1Score > 34 indicates identity
Score > 20 indicates homology
VPAVIGK
1657 616.3457 1230.6768 1230.6754 1.15 1 13 0.81 +1Score > 40 indicates identity
Score > 24 indicates homology
NLATKIVCVGR + Deamidated (NQ)
2897 457.2449 1824.9505 1824.9833 -18.0 0 13 0.96 +1Score > 39 indicates identity
Score > 25 indicates homology
ALQLTAPDLLSQGIIDR + 2 Deamidated (NQ)
1729 637.8112 1273.6078 1273.5972 8.33 1 13 0.5 +1Score > 41 indicates identity
Score > 22 indicates homology
QPIMQGDTEQK
2467 816.4149 1630.8152 1630.8349 -12.0 0 13 0.85 +1Score > 41 indicates identity
Score > 25 indicates homology
VSIVGSGMISNPGVAAR + Deamidated (NQ); Oxidation (M)
2494 547.2990 1638.8752 1638.8940 -11.5 1 13 0.36 +1Score > 38 indicates identity
Score > 21 indicates homology
LDQALENAAIKPSIR + Deamidated (NQ)
3797 1190.6396 2379.2646 2379.2355 12.2 1 13 0.83 +1Score > 36 indicates identity
Score > 24 indicates homology
TNLGNFLSKINMNTIDIQITK + 2 Deamidated (NQ)
1909 684.3513 1366.6880 1366.6915 -2.51 0 13 0.69 +1Score > 40 indicates identity
Score > 24 indicates homology
GTYVCIATNALGK
2984 627.3363 1878.9871 1878.9772 5.25 1 13 0.86 +1Score > 38 indicates identity
Score > 25 indicates homology
ALIASSNNAHIRGNQASR
1596 601.3397 1200.6648 1200.6601 3.92 1 13 0.3 +1Score > 40 indicates identity
Score > 20 indicates homology
DLVQQKDILK + 2 Deamidated (NQ)
2107 735.3703 1468.7260 1468.7521 -17.8 0 13 0.58 +1Score > 40 indicates identity
Score > 23 indicates homology
QIQAVPIANSQAAR + 3 Deamidated (NQ)
3134 996.0161 1990.0176 1990.0081 4.80 1 13 0.36 +1Score > 40 indicates identity
Score > 21 indicates homology
QTTEPNYPEMLLIAINK + Oxidation (M)
1645 410.2277 1227.6613 1227.6684 -5.79 1 13 0.96 +1Score > 40 indicates identity
Score > 25 indicates homology
GVDLAQARVSGR
3765 782.0862 2343.2368 2343.2395 -1.18 0 13 0.41 +1Score > 36 indicates identity
Score > 21 indicates homology
QLYVNLVDSPLAMTDLPLALR + 2 Deamidated (NQ)
2596 560.3179 1677.9319 1677.9090 13.6 1 13 0.15 +1Score > 36 indicates identity
Score > 17 indicates homology
NFELTGRPVVGTIFK + Deamidated (NQ)
1813 439.8832 1316.6278 1316.6217 4.63 0 13 0.86 +1Score > 41 indicates identity
Score > 25 indicates homology
DLLGTNMMLHR + Deamidated (NQ); Oxidation (M)
3065 645.6933 1934.0581 1934.0262 16.5 0 13 0.086 +1Score > 36 indicates identity
Score > 14 indicates homology
SNQPHTFVIISLDPPIR + Deamidated (NQ)
1313 360.8548 1079.5426 1079.5355 6.57 1 13 0.46 +1Score > 41 indicates identity
Score > 22 indicates homology
KQQVAMMVK + 2 Deamidated (NQ); Oxidation (M)
1321 362.5459 1084.6159 1084.5989 15.6 1 13 0.99 +1Score > 40 indicates identity
Score > 25 indicates homology
TDAPAVTRVR
2263 768.4148 1534.8150 1534.8355 -13.3 1 13 0.8 +1Score > 39 indicates identity
Score > 24 indicates homology
NLGITTYGVLSLRE
3049 962.4586 1922.9026 1922.8900 6.57 0 13 0.21 +1Score > 40 indicates identity
Score > 18 indicates homology
QVAMAGDGQVTMGQATVMK + Deamidated (NQ)
3461 1078.0295 2154.0444 2154.0561 -5.43 1 13 0.95 +1Score > 39 indicates identity
Score > 25 indicates homology
MPDQPATACPLVLASASPRR + Deamidated (NQ); Oxidation (M)
1855 671.9117 1341.8088 1341.8232 -10.7 1 13 0.28 +1Score > 31 indicates identity
Score > 20 indicates homology
LSLKQTDVVIVK
2687 863.9897 1725.9648 1725.9512 7.90 1 13 0.75 +1Score > 36 indicates identity
Score > 24 indicates homology
ADLAAATQAAIVEVLAAK + Deamidated (NQ)
1801 437.2231 1308.6475 1308.6721 -18.8 1 13 0.66 +1Score > 41 indicates identity
Score > 23 indicates homology
RHTKPNPMQGK + Oxidation (M)
3998 862.4536 2584.3390 2584.3393 -0.13 1 13 0.26 +1Score > 37 indicates identity
Score > 19 indicates homology
VDPVNFKLLSQCIQVVLAAHMGK + 2 Deamidated (NQ); Oxidation (M)
1829 441.9152 1322.7238 1322.7307 -5.20 0 12 0.64 +1Score > 38 indicates identity
Score > 23 indicates homology
DAAVVVVSSAIHR
2161 498.9244 1493.7514 1493.7296 14.5 0 12 0.89 +1Score > 41 indicates identity
Score > 24 indicates homology
MSQLSNPSQAFLR + Oxidation (M)
3228 680.3416 2038.0030 2037.9639 19.2 1 12 0.12 +1Score > 40 indicates identity
Score > 16 indicates homology
MVQVVYPNMADLEIGNVK + 3 Deamidated (NQ); Oxidation (M)
3269 687.3584 2059.0534 2059.0143 19.0 1 12 0.67 +1Score > 39 indicates identity
Score > 23 indicates homology
TTDLQTTLQLSMKAIQHE + 2 Deamidated (NQ)
2302 779.8885 1557.7624 1557.7674 -3.21 1 12 0.98 +1Score > 40 indicates identity
Score > 25 indicates homology
TNTIPNWIKLNNE + 2 Deamidated (NQ)
3746 776.4138 2326.2196 2326.1804 16.8 1 12 0.64 +1Score > 37 indicates identity
Score > 23 indicates homology
LGNNLPTPQEVVALYNQANIR + 3 Deamidated (NQ)
3620 752.0375 2253.0907 2253.1285 -16.8 1 12 0.84 +1Score > 39 indicates identity
Score > 24 indicates homology
WNLLLQYRNSTLNMLNMK + 2 Deamidated (NQ)
1013 314.5246 940.5520 940.5494 2.71 1 12 0.61 +1Score > 39 indicates identity
Score > 23 indicates homology
VKHPIYGK
945 458.2774 914.5402 914.5297 11.5 1 12 0.66 +1Score > 40 indicates identity
Score > 23 indicates homology
IATIRNAR + Deamidated (NQ)
2822 895.9604 1789.9062 1789.9349 -16.0 1 12 0.6 +1Score > 40 indicates identity
Score > 23 indicates homology
LDLYALENILNQQIK + 3 Deamidated (NQ)
2971 936.5234 1871.0322 1870.9975 18.6 1 12 0.81 +1Score > 35 indicates identity
Score > 24 indicates homology
NILLTFAQMNPKNPIR + 2 Deamidated (NQ)
2730 872.4312 1742.8478 1742.8435 2.52 0 12 0.71 +1Score > 40 indicates identity
Score > 23 indicates homology
NIQDILAQNLSGGQNR + 3 Deamidated (NQ)
3054 963.4803 1924.9460 1924.9677 -11.2 1 12 0.54 +1Score > 40 indicates identity
Score > 22 indicates homology
TVQSTIIQPTIDGMRHE
3152 667.3284 1998.9634 1998.9721 -4.35 1 12 0.42 +1Score > 40 indicates identity
Score > 21 indicates homology
LGTNIRIFVDSPGTMYAE + Oxidation (M)
784 849.4680 848.4607 848.4505 12.1 0 12 0.67 +1Score > 40 indicates identity
Score > 23 indicates homology
QVVSNFR
3654 760.3960 2278.1662 2278.2072 -18.0 0 12 0.15 +1Score > 38 indicates identity
Score > 17 indicates homology
SSAALFVSFMINLFVTAVFAK + Oxidation (M)
3930 835.7703 2504.2891 2504.2590 12.0 1 12 0.84 +1Score > 37 indicates identity
Score > 24 indicates homology
ISQTFSGIMTMLNMQFLVRVR + Deamidated (NQ); 2 Oxidation (M)
378 652.3913 651.3840 651.3816 3.66 0 12 0.94 +1Score > 34 indicates identity
Score > 25 indicates homology
GHIAVR
939 457.2431 912.4716 912.4665 5.65 0 12 0.88 +1Score > 39 indicates identity
Score > 24 indicates homology
GGEPVADLR
1500 580.3579 1158.7012 1158.7046 -2.88 1 12 0.26 +1Score > 32 indicates identity
Score > 19 indicates homology
SLPLLMITKK + Oxidation (M)
3638 755.7473 2264.2201 2264.2012 8.35 1 12 0.17 +1Score > 36 indicates identity
Score > 17 indicates homology
LQNLQHLAITLSAELSLNQR + 3 Deamidated (NQ)
481 352.2224 702.4302 702.4276 3.80 0 12 0.67 +1Score > 38 indicates identity
Score > 23 indicates homology
ITQTIK
932 455.3016 908.5886 908.5807 8.69 0 12 0.84 +1Score > 27 indicates identity
Score > 24 indicates homology
IGGVPVVIR
2932 925.4485 1848.8824 1848.9026 -10.9 1 12 0.23 +1Score > 40 indicates identity
Score > 18 indicates homology
GQLSSVTAVLQEMNLLE + 2 Deamidated (NQ); Oxidation (M)
1337 365.2091 1092.6055 1092.6040 1.37 1 12 0.38 +1Score > 37 indicates identity
Score > 21 indicates homology
AALAGGHEIVR
2516 825.9293 1649.8440 1649.8426 0.86 1 12 0.51 +1Score > 40 indicates identity
Score > 22 indicates homology
ITWWHKLDPANNR
536 366.2194 730.4242 730.4337 -13.0 0 12 0.96 +1Score > 43 indicates identity
Score > 25 indicates homology
AISTAIR
3558 1105.5762 2209.1378 2209.0984 17.9 0 12 0.17 +1Score > 38 indicates identity
Score > 17 indicates homology
IPDHNTGQMIGVTPNSLMIR + Oxidation (M)
4153 927.4618 2779.3636 2779.3718 -2.96 1 12 0.17 +1Score > 38 indicates identity
Score > 17 indicates homology
SKTQFVVDAVYAFAYALHNLHNDR + Deamidated (NQ)
1634 408.2354 1221.6844 1221.6830 1.15 1 12 0.96 +1Score > 37 indicates identity
Score > 25 indicates homology
GVQKVHIIDGR + Deamidated (NQ)
3312 1044.5095 2087.0044 2086.9656 18.6 1 12 0.34 +1Score > 39 indicates identity
Score > 20 indicates homology
ALDTYNEINAVMNLVLFE + 3 Deamidated (NQ); Oxidation (M)
2702 866.8893 1731.7640 1731.7807 -9.64 0 12 0.66 +1Score > 38 indicates identity
Score > 23 indicates homology
NDNPLVDMQAMSPTAK + Deamidated (NQ)
2728 871.9961 1741.9776 1741.9726 2.88 1 12 0.84 +1Score > 35 indicates identity
Score > 24 indicates homology
QIQLLLQYLKNDPR + Deamidated (NQ)
503 358.7013 715.3880 715.3864 2.24 0 12 0.89 +1Score > 44 indicates identity
Score > 24 indicates homology
QSSGLPK
2033 712.4032 1422.7918 1422.7718 14.1 1 12 0.91 +1Score > 37 indicates identity
Score > 24 indicates homology
IQVGKNEPVDPVK + Deamidated (NQ)
2512 825.9264 1649.8382 1649.8447 -3.91 0 12 0.81 +1Score > 40 indicates identity
Score > 24 indicates homology
TLSGTINPGLVQMFR + Deamidated (NQ); Oxidation (M)
2996 630.3397 1887.9973 1887.9942 1.64 1 12 0.2 +1Score > 38 indicates identity
Score > 18 indicates homology
FGRLVQLQTLILQDNE + 2 Deamidated (NQ)
1799 655.3304 1308.6462 1308.6422 3.10 1 12 0.62 +1Score > 41 indicates identity
Score > 23 indicates homology
KSANNAHQVPDK + Deamidated (NQ)
3042 480.2441 1916.9473 1916.9130 17.9 0 12 0.66 +1Score > 40 indicates identity
Score > 23 indicates homology
GVSGFPGADGIPGHPGQGGPR + Deamidated (NQ)
4001 863.7781 2588.3125 2588.3342 -8.40 0 12 0.09 +1Score > 37 indicates identity
Score > 14 indicates homology
AGLSAAGADVMLLGPMPTPAIAYLTR + 2 Oxidation (M)
2032 712.3901 1422.7656 1422.7402 17.9 1 12 0.21 +1Score > 38 indicates identity
Score > 18 indicates homology
QEIVHVCQAIAR
4030 659.8138 2635.2261 2635.2186 2.83 1 12 0.16 +1Score > 37 indicates identity
Score > 17 indicates homology
GQTVFMGDFPLMTDKGTFIINGTE + Deamidated (NQ); Oxidation (M)
3684 1149.6432 2297.2718 2297.2644 3.23 1 12 0.081 +1Score > 33 indicates identity
Score > 14 indicates homology
SLGLELVGAVFHTNQHPPILR
1716 633.8346 1265.6546 1265.6503 3.44 1 12 0.81 +1Score > 40 indicates identity
Score > 24 indicates homology
ITYTLIGENNK + Deamidated (NQ)
3395 1064.5618 2127.1090 2127.0749 16.1 1 12 0.82 +1Score > 38 indicates identity
Score > 24 indicates homology
IHSTFTIQVEYGTGPHAIR + Deamidated (NQ)
4449 1055.8452 3164.5138 3164.5122 0.48 1 12 0.42 +1Score > 36 indicates identity
Score > 21 indicates homology
LAATFQSDIPSKQNQAQNGNQSHLPPQSR + 3 Deamidated (NQ)
1632 408.1895 1221.5467 1221.5587 -9.86 1 12 0.82 +1Score > 40 indicates identity
Score > 24 indicates homology
QFMPELLAQE + Deamidated (NQ); Oxidation (M)
2380 531.2817 1590.8233 1590.8478 -15.4 0 12 0.69 +1Score > 40 indicates identity
Score > 23 indicates homology
ISQGNHLPQSILQR + Deamidated (NQ)
2410 805.3782 1608.7418 1608.7600 -11.3 1 12 0.67 +1Score > 40 indicates identity
Score > 23 indicates homology
KMGMNIINDDITGR + 2 Oxidation (M)
1847 446.2599 1335.7579 1335.7663 -6.30 1 12 0.92 +1Score > 35 indicates identity
Score > 24 indicates homology
HFLLEVAAAPLR
3386 708.0725 2121.1957 2121.1649 14.5 0 12 0.59 +1Score > 32 indicates identity
Score > 22 indicates homology
IDFIQVIIYIGFPNLLIE + 2 Deamidated (NQ)
3944 631.0957 2520.3537 2520.3071 18.5 1 12 0.16 +1Score > 35 indicates identity
Score > 17 indicates homology
VIQQALEQSGLPAAAVQAIDKPDR + 3 Deamidated (NQ)
505 717.3850 716.3777 716.3817 -5.53 0 12 0.64 +1Score > 43 indicates identity
Score > 23 indicates homology
NRPTTK + Deamidated (NQ)
2869 906.0317 1810.0488 1810.0352 7.52 1 12 0.39 +1Score > 31 indicates identity
Score > 20 indicates homology
EPIFVIAELNLIGLNR
2713 867.4758 1732.9370 1732.9260 6.36 1 12 0.74 +1Score > 38 indicates identity
Score > 23 indicates homology
HYSIARQVQYVLQK + Deamidated (NQ)
2378 531.2798 1590.8176 1590.7963 13.3 0 12 0.94 +1Score > 40 indicates identity
Score > 24 indicates homology
LLDPPTLAMGSFDAK + Oxidation (M)
3166 1005.5526 2009.0906 2009.0550 17.7 1 12 0.23 +1Score > 36 indicates identity
Score > 18 indicates homology
NPMHVVKAALIGADVATMR + Oxidation (M)
1740 320.1833 1276.7041 1276.7247 -16.1 0 12 0.95 +1Score > 38 indicates identity
Score > 24 indicates homology
IIMILSMALTR + Oxidation (M)
2792 591.6389 1771.8949 1771.8880 3.89 0 12 0.24 +1Score > 40 indicates identity
Score > 18 indicates homology
DPDTVLLLANATLTWE + Deamidated (NQ)
1232 346.8469 1037.5189 1037.5142 4.54 1 12 0.88 +1Score > 41 indicates identity
Score > 24 indicates homology
VSTGENLYR
2028 712.3592 1422.7038 1422.7143 -7.37 1 12 0.98 +1Score > 41 indicates identity
Score > 24 indicates homology
LFVDGGEVAFVNR + Deamidated (NQ)
1315 541.7524 1081.4902 1081.4788 10.6 1 12 0.84 +1Score > 40 indicates identity
Score > 24 indicates homology
QYRASDNAR + 2 Deamidated (NQ)
1504 580.3604 1158.7062 1158.6860 17.5 0 12 0.13 +1Score > 31 indicates identity
Score > 16 indicates homology
ILDTVISSIAK
2375 795.4265 1588.8384 1588.8130 16.0 0 12 0.6 +1Score > 40 indicates identity
Score > 22 indicates homology
QLMLNALGQGTANLK + 2 Deamidated (NQ); Oxidation (M)
2448 540.2949 1617.8629 1617.8727 -6.04 1 12 0.73 +1Score > 39 indicates identity
Score > 23 indicates homology
GQGTTFVVTLPVKDR + Deamidated (NQ)
3311 696.0250 2085.0532 2085.0142 18.7 1 12 0.8 +1Score > 39 indicates identity
Score > 24 indicates homology
MNSFRGAFLIWAVATWAE + Oxidation (M)
4323 998.4928 2992.4566 2992.5150 -19.5 0 12 0.27 +1Score > 37 indicates identity
Score > 19 indicates homology
ALCAQTMLVSHPGVNVLLLDDGLQHYK + Deamidated (NQ)
2177 749.9380 1497.8614 1497.8667 -3.53 1 12 0.83 +1Score > 35 indicates identity
Score > 24 indicates homology
RVFQAAQALGIPVK + Deamidated (NQ)
3598 1122.1072 2242.1998 2242.1555 19.8 1 12 0.75 +1Score > 36 indicates identity
Score > 23 indicates homology
IAAEILSADVWPISAAIMQNK + 2 Deamidated (NQ)
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