MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1–100 (out of 3698)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
298 601.3688 600.3615 600.3707 -15.3 1 43 0.061 +1Score > 44 indicates identity AGRVAK
1094 972.5245 971.5172 971.5148 2.46 0 42 0.054 +1Score > 42 indicates identity SAGLQVQNR
827 430.7229 859.4312 859.4174 16.1 1 41 0.1 +1Score > 43 indicates identity IIQENIE + 2 Deamidated (NQ)
307 604.3674 603.3601 603.3592 1.60 0 40 0.052 +1Score > 40 indicates identity
Score > 40 indicates homology		 ISSGLK
502 358.2283 714.4420 714.4388 4.51 0 40 0.071 -1Score > 41 indicates identity ITGVVAR
873 443.7603 885.5060 885.5144 -9.45 1 40 0.084 +1Score > 41 indicates identity INQSIRR
1154 501.2867 1000.5588 1000.5553 3.56 1 40 0.077 +1Score > 41 indicates identity QATLEQALK
268 572.3781 571.3708 571.3693 2.63 0 39 0.069 +1Score > 40 indicates identity AGIAIK
565 745.4592 744.4519 744.4494 3.43 0 39 0.095 +1Score > 41 indicates identity ITAALTR
591 761.4062 760.3989 760.3967 2.97 0 38 0.17 +1Score > 43 indicates identity ATDVNLK + Deamidated (NQ)
278 586.3677 585.3604 585.3598 1.01 0 38 0.077 +1Score > 39 indicates identity AGIVAR
1247 524.2585 1046.5024 1046.4880 13.8 1 38 0.12 +1Score > 41 indicates identity NTELDLANR + 2 Deamidated (NQ)
260 558.3622 557.3549 557.3537 2.23 0 37 0.12 +1Score > 40 indicates identity AGAVLK
347 315.7018 629.3890 629.3861 4.74 0 37 0.094 +1Score > 41 indicates identity
Score > 39 indicates homology		 VAVVSR
619 775.4582 774.4509 774.4487 2.83 0 37 0.09 +1Score > 39 indicates identity TVDTVIK
601 383.6913 765.3680 765.3657 3.06 1 37 0.18 +1Score > 42 indicates identity VESFQR + Deamidated (NQ)
1123 494.7581 987.5016 987.4985 3.17 1 37 0.072 +1Score > 42 indicates identity
Score > 38 indicates homology		 QELTDVQR
344 629.3636 628.3563 628.3544 3.04 0 37 0.06 +1Score > 37 indicates identity AVNPTK
540 732.4055 731.3982 731.3926 7.74 1 37 0.15 +1Score > 41 indicates identity RSAQAAK + Deamidated (NQ)
828 430.7469 859.4792 859.4651 16.5 0 37 0.056 +1Score > 42 indicates identity
Score > 37 indicates homology		 IVDSNIAK + Deamidated (NQ)
1357 551.3006 1100.5866 1100.5866 0.049 1 37 0.069 +1Score > 42 indicates identity
Score > 38 indicates homology		 LVLTKNFHE + Deamidated (NQ)
1170 505.2541 1008.4936 1008.4950 -1.35 0 37 0.18 +1Score > 42 indicates identity QFLDVMQK + Deamidated (NQ)
610 386.2402 770.4658 770.4650 1.09 1 37 0.088 +1Score > 41 indicates identity
Score > 39 indicates homology		 VIAEALR
1258 528.7947 1055.5748 1055.5723 2.39 1 37 0.099 +1Score > 39 indicates identity LQAQAQRLE
479 352.2043 702.3940 702.3912 4.07 0 36 0.2 +1Score > 43 indicates identity
Score > 42 indicates homology		 VTANLGK + Deamidated (NQ)
469 351.2025 700.3904 700.3868 5.24 1 36 0.22 +1Score > 42 indicates identity IGQEVR
1032 474.2393 946.4640 946.4607 3.53 0 36 0.097 +1Score > 42 indicates identity
Score > 38 indicates homology		 AQVQAQTAK + 3 Deamidated (NQ)
367 645.3320 644.3247 644.3242 0.88 1 36 0.15 +1Score > 40 indicates identity EAQAAR
1150 500.7761 999.5376 999.5349 2.75 0 36 0.12 +1Score > 40 indicates identity
Score > 39 indicates homology		 VANLGNLGDK
350 632.3749 631.3676 631.3653 3.66 0 36 0.21 +1Score > 41 indicates identity ATALTR
581 379.2329 756.4512 756.4494 2.48 0 36 0.21 +1Score > 41 indicates identity AVALIDR
475 351.7060 701.3974 701.3959 2.14 1 35 0.35 +1Score > 43 indicates identity VEVQVK + Deamidated (NQ)
221 504.2787 503.2714 503.2704 2.12 0 35 0.73 +1Score > 46 indicates identity SVNGK
1041 949.5016 948.4943 948.4916 2.85 0 35 0.25 +1Score > 41 indicates identity DIFANGALK + Deamidated (NQ)
409 672.4059 671.3986 671.3966 2.98 0 35 0.4 +1Score > 43 indicates identity TVSIPR
1122 494.2982 986.5818 986.6012 -19.6 1 35 0.14 +1Score > 39 indicates identity TILAETIVK
976 928.4176 927.4103 927.3942 17.3 1 35 0.11 +1Score > 38 indicates identity
Score > 38 indicates homology		 EFMMQSR
1356 551.3001 1100.5856 1100.5826 2.77 0 34 0.11 +1Score > 41 indicates identity
Score > 37 indicates homology		 AGDLVIDGSVR
847 437.2628 872.5110 872.4967 16.4 1 34 0.23 +1Score > 40 indicates identity QALAELTK
616 388.2144 774.4142 774.4236 -12.0 1 34 0.079 +1Score > 43 indicates identity
Score > 36 indicates homology		 ALSVETR
783 425.2278 848.4410 848.4392 2.17 0 34 0.11 +1Score > 42 indicates identity
Score > 37 indicates homology		 ALQDAGFK
705 407.7529 813.4912 813.5072 -19.6 0 34 0.17 +1Score > 41 indicates identity
Score > 39 indicates homology		 NIILVSR
416 337.2038 672.3930 672.3919 1.77 0 34 0.11 +1Score > 43 indicates identity
Score > 37 indicates homology		 SIGIQR
1172 337.5265 1009.5577 1009.5379 19.6 0 34 0.093 +1Score > 37 indicates identity
Score > 36 indicates homology		 TAPGAPMLPR
1766 430.5886 1288.7440 1288.7391 3.80 0 34 0.074 +1Score > 35 indicates identity TFSIIKPDAVAK
909 450.2857 898.5568 898.5488 9.01 0 33 0.12 +1Score > 37 indicates identity AVLDQLLK
340 628.3691 627.3618 627.3592 4.22 1 33 0.084 +1Score > 37 indicates identity
Score > 35 indicates homology		 EVPVGK
851 437.7541 873.4936 873.4920 1.93 0 33 0.38 +1Score > 42 indicates identity AQITTLAR + Deamidated (NQ)
870 442.2367 882.4588 882.4559 3.30 0 33 0.21 +1Score > 39 indicates identity SNVDVPPR
740 415.7420 829.4694 829.4810 -13.9 1 33 0.57 +1Score > 43 indicates identity KIIDWR
1711 631.3530 1260.6914 1260.6747 13.3 1 33 0.23 +1Score > 39 indicates identity MKLLNQLIDR + 2 Deamidated (NQ); Oxidation (M)
1309 539.8002 1077.5858 1077.5926 -6.26 1 33 0.14 +1Score > 40 indicates identity
Score > 37 indicates homology		 KMLADLMIK + Oxidation (M)
685 401.2469 800.4792 800.4756 4.59 0 33 0.42 +1Score > 42 indicates identity IATNLLR + Deamidated (NQ)
1224 1035.5881 1034.5808 1034.6012 -19.7 1 33 0.14 +1Score > 38 indicates identity
Score > 37 indicates homology		 IEIYIVGTK
1263 529.7905 1057.5664 1057.5768 -9.76 0 33 0.11 +1Score > 41 indicates identity
Score > 36 indicates homology		 LSTLDASPVR
741 415.7469 829.4792 829.4657 16.3 1 33 0.16 +1Score > 43 indicates identity
Score > 37 indicates homology		 KDIADLR
743 416.2467 830.4788 830.4749 4.74 1 33 0.31 +1Score > 40 indicates identity TQVELLK + Deamidated (NQ)
1078 481.7651 961.5156 961.5233 -7.93 0 33 0.071 +1Score > 41 indicates identity
Score > 34 indicates homology		 QGGAQIFLK + Deamidated (NQ)
443 344.2242 686.4338 686.4327 1.73 0 33 0.09 +1Score > 40 indicates identity
Score > 35 indicates homology		 SVGAILK
562 372.2255 742.4364 742.4225 18.8 0 33 0.27 +1Score > 42 indicates identity
Score > 39 indicates homology		 VLAAQLE
854 438.2470 874.4794 874.4760 3.98 1 32 0.44 +1Score > 41 indicates identity TIENLSAK
1425 1131.5677 1130.5604 1130.5567 3.25 1 32 0.28 +1Score > 42 indicates identity
Score > 39 indicates homology		 VVELNASDQR + Deamidated (NQ)
459 347.2014 692.3882 692.3857 3.64 1 32 0.14 +1Score > 39 indicates identity
Score > 36 indicates homology		 FGAKLGT
858 879.4956 878.4883 878.4861 2.48 1 32 0.26 +1Score > 39 indicates identity KAFSDAIK
1473 576.7715 1151.5284 1151.5260 2.09 1 32 0.089 +1Score > 41 indicates identity
Score > 34 indicates homology		 NYWNGRWR + Deamidated (NQ)
1008 470.7905 939.5664 939.5753 -9.42 0 32 0.13 +1Score > 36 indicates identity QPVLLITR + Deamidated (NQ)
404 671.3745 670.3672 670.3802 -19.4 0 32 0.16 +1Score > 36 indicates identity WGALPK
908 450.2856 898.5566 898.5600 -3.69 1 32 0.18 +1Score > 37 indicates identity IGINKNIK
1207 516.2609 1030.5072 1030.4931 13.7 0 32 0.51 +1Score > 41 indicates identity LTQNLAGGQE + Deamidated (NQ)
1598 602.7956 1203.5766 1203.5771 -0.42 1 32 0.11 +1Score > 41 indicates identity
Score > 35 indicates homology		 WLQEQGSDLK + Deamidated (NQ)
669 398.2228 794.4310 794.4286 3.04 0 32 0.31 +1Score > 39 indicates identity
Score > 39 indicates homology		 AVSSYLR
381 656.4137 655.4064 655.4017 7.23 0 32 0.072 +1Score > 33 indicates identity AISIPR
380 328.7031 655.3916 655.3905 1.82 0 31 0.15 +1Score > 37 indicates identity
Score > 36 indicates homology		 AIDLPK
1115 982.4742 981.4669 981.4556 11.6 1 31 0.52 +1Score > 41 indicates identity WEQYVTR + Deamidated (NQ)
595 763.4112 762.4039 762.4024 1.97 1 31 0.24 +1Score > 43 indicates identity
Score > 37 indicates homology		 FLEGAAR
1588 399.8962 1196.6668 1196.6877 -17.5 1 31 0.29 +1Score > 38 indicates identity DRIVTPLNIR + Deamidated (NQ)
742 416.2460 830.4774 830.4749 3.05 1 31 0.33 +1Score > 41 indicates identity
Score > 39 indicates homology		 TLEQIVK + Deamidated (NQ)
782 424.6999 847.3852 847.3937 -9.93 1 31 0.1 +1Score > 41 indicates identity
Score > 33 indicates homology		 DDTWRR
382 329.7105 657.4064 657.4061 0.51 1 31 0.069 +1Score > 41 indicates identity
Score > 32 indicates homology		 IEIVGK
1606 606.3451 1210.6756 1210.6921 -13.6 1 31 0.28 +1Score > 38 indicates identity TRAILDALPLE
2630 567.3210 1698.9412 1698.9193 12.9 1 31 0.22 +1Score > 37 indicates identity TIPEFAPGDTVVVQVK
613 386.7264 771.4382 771.4239 18.6 0 31 0.45 +1Score > 43 indicates identity
Score > 40 indicates homology		 SSVLNPR
617 775.4332 774.4259 774.4236 3.04 0 31 0.76 +1Score > 43 indicates identity
Score > 42 indicates homology		 GNSLTGVK
1288 536.2947 1070.5748 1070.5720 2.65 0 31 0.23 +1Score > 40 indicates identity
Score > 37 indicates homology		 QLDLGAVQAR + Deamidated (NQ)
1158 1003.5561 1002.5488 1002.5570 -8.16 1 31 0.56 +1Score > 41 indicates identity ASRISNLSR
577 377.2173 752.4200 752.4181 2.62 0 30 0.12 +1Score > 38 indicates identity
Score > 34 indicates homology		 ALDVHAK
1379 557.8100 1113.6054 1113.6030 2.23 1 30 0.15 +1Score > 40 indicates identity
Score > 35 indicates homology		 IAGTEAGISAPK
1529 586.8380 1171.6614 1171.6812 -16.9 1 30 0.44 +1Score > 39 indicates identity KNLDDLTLLK
775 423.2289 844.4432 844.4515 -9.76 1 30 0.74 +1Score > 43 indicates identity
Score > 42 indicates homology		 LAGSADRR
652 789.4056 788.3983 788.3963 2.58 0 30 0.36 +1Score > 44 indicates identity
Score > 38 indicates homology		 VAQMANR
1160 1004.5444 1003.5371 1003.5338 3.28 0 30 0.37 +1Score > 41 indicates identity
Score > 38 indicates homology		 YVIGQEPAK
704 407.7516 813.4886 813.4960 -9.04 0 30 0.36 +1Score > 39 indicates identity
Score > 38 indicates homology		 LGADVLVK
897 894.4714 893.4641 893.4607 3.87 1 30 0.76 +1Score > 41 indicates identity GISEGLYR
761 421.2623 840.5100 840.5069 3.77 1 30 0.19 +1Score > 35 indicates identity QLKDIPK
1147 499.3045 996.5944 996.5855 8.95 1 30 0.19 +1Score > 35 indicates identity EVIAIDLPK
1028 472.7947 943.5748 943.5702 4.90 1 30 0.12 +1Score > 36 indicates identity
Score > 33 indicates homology		 TITVLIER
1210 516.2617 1030.5088 1030.4931 15.3 0 30 0.13 +1Score > 42 indicates identity
Score > 34 indicates homology		 LTQNLAGGQE + Deamidated (NQ)
826 430.2550 858.4954 858.4811 16.8 1 30 0.82 +1Score > 42 indicates identity AATLEAGVK
931 454.7469 907.4792 907.4651 15.6 1 30 0.45 +1Score > 41 indicates identity
Score > 39 indicates homology		 GANLEYLK + Deamidated (NQ)
588 380.2535 758.4924 758.4902 3.00 0 30 0.054 +1Score > 30 indicates identity VISILSK
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