MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 91–100 (out of 230)

Page: Previous 1 …5 6 7 8 9 10 11 12 13 14 15 … 23 Next 

+91

Accession Score Description
1 CH10_PSE14 71 10 kDa chaperonin OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=groS PE=3 SV=1

+92

Accession Score Description
1 HSLU_PSEPG 67 ATP-dependent hsl protease ATP-binding subunit hslU OS=Pseudomonas putida (strain GB-1) GN=hslU PE=3 SV=1

+93

Accession Score Description
1 RL29_PSE14 67 50S ribosomal protein L29 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpmC PE=3 SV=1

+94

Accession Score Description
1 FLAE_VIBCH 67 Flagellin E OS=Vibrio cholerae GN=flaE PE=3 SV=1

+95

Accession Score Description
1 CLPB_PSEPK 65 Chaperone protein clpB OS=Pseudomonas putida (strain KT2440) GN=clpB PE=3 SV=1

+96

Accession Score Description
Family member distances as a dendrogram 1 XYLA_ECOBW 65 Xylose isomerase OS=Escherichia coli (strain K12 / BW2952) GN=xylA PE=3 SV=1
2 XYLA_ENT38 53 Xylose isomerase OS=Enterobacter sp. (strain 638) GN=xylA PE=3 SV=1

+97

Accession Score Description
1 RS6_AZOVD 64 30S ribosomal protein S6 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsF PE=3 SV=1

-98

Accession Score Description
1 PURA_PSEPG 64 Adenylosuccinate synthetase OS=Pseudomonas putida (strain GB-1) GN=purA PE=3 SV=1
Score Mass Matches Sequences emPAI
98.1 PURA_PSEPG 64 47089 3 (2) 3 (2) 0.10
Adenylosuccinate synthetase OS=Pseudomonas putida (strain GB-1) GN=purA PE=3 SV=1
4 samesets of PURA_PSEPG
PURA_PSEPK 64 47089 3 (2) 3 (2) 0.10
Adenylosuccinate synthetase OS=Pseudomonas putida (strain KT2440) GN=purA PE=3 SV=1
PURA_PSEPW 64 46962 3 (2) 3 (2) 0.10
Adenylosuccinate synthetase OS=Pseudomonas putida (strain W619) GN=purA PE=3 SV=1
PURA_PSEP1 64 47089 3 (2) 3 (2) 0.10
Adenylosuccinate synthetase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=purA PE=3 SV=1
PURA_PSEE4 64 47045 3 (2) 3 (2) 0.10
Adenylosuccinate synthetase OS=Pseudomonas entomophila (strain L48) GN=purA PE=3 SV=1

-3 peptide matches (3 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1817   440.2812 1317.8218 1317.8132 6.47 0 44 0.0012 +1Score > 27 indicates identity U K.TVLHLIPSGILR.E
2211   502.9627 1505.8663 1505.8566 6.44 1 51 0.00097 +1Score > 33 indicates identity U K.IVDLLTEHAAAVVR.Y
3363   1058.5336 2115.0526 2115.0340 8.80 1 3 1.5 +6Score > 39 indicates identity
Score > 17 indicates homology		 U R.EGVQCLIGNGVVVAPDALMR.E + 2 Deamidated (NQ); Oxidation (M)

2 subsets and intersections (123 subset proteins in total)

Score Mass Subset of
PURA_PSEF5 51 47146 98.1
Adenylosuccinate synthetase OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=purA PE=3 SV=1
2 samesets of PURA_PSEF5
PURA_PSEFS 51 46877
Adenylosuccinate synthetase OS=Pseudomonas fluorescens (strain SBW25) GN=purA PE=3 SV=1
PURA_PSEPF 51 46858
Adenylosuccinate synthetase OS=Pseudomonas fluorescens (strain Pf0-1) GN=purA PE=3 SV=1
PURA2_PHOLL 44 47439 98.1
Adenylosuccinate synthetase 2 OS=Photorhabdus luminescens subsp. laumondii GN=purA2 PE=3 SV=1
+119 samesets of PURA2_PHOLL

+99

Accession Score Description
1 SUCC_COXBN 63 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Coxiella burnetii (strain Dugway 5J108-111) GN=sucC PE=3 SV=1

+100

Accession Score Description
1 ATP6_PSEP1 63 ATP synthase subunit a OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=atpB PE=3 SV=1
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