MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 91–100 (out of 230)


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+91

Accession Score Description
1 CH10_PSE14 71 10 kDa chaperonin OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=groS PE=3 SV=1

+92

Accession Score Description
1 HSLU_PSEPG 67 ATP-dependent hsl protease ATP-binding subunit hslU OS=Pseudomonas putida (strain GB-1) GN=hslU PE=3 SV=1

+93

Accession Score Description
1 RL29_PSE14 67 50S ribosomal protein L29 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpmC PE=3 SV=1

-94

Accession Score Description
1 FLAE_VIBCH 67 Flagellin E OS=Vibrio cholerae GN=flaE PE=3 SV=1
Score Mass Matches Sequences emPAI
94.1 FLAE_VIBCH 67 41017 5 (2) 4 (2) 0.12
Flagellin E OS=Vibrio cholerae GN=flaE PE=3 SV=1
15 samesets of FLAE_VIBCH
FLAE_VIBC3 67 41017 5 (2) 4 (2) 0.12
Flagellin E OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) GN=flaE PE=3 SV=1
FLAB_VIBPA 67 40149 3 (2) 3 (2) 0.12
Polar flagellin B/D OS=Vibrio parahaemolyticus GN=flaB PE=3 SV=2
FLAA_VIBPA 67 39825 4 (2) 3 (2) 0.12
Polar flagellin A OS=Vibrio parahaemolyticus GN=flaA PE=3 SV=1
FLAE_VIBAN 67 40757 3 (2) 3 (2) 0.12
Probable flagellin E OS=Vibrio anguillarum GN=flaE PE=3 SV=1
FLAF_VIBPA 67 40476 3 (2) 3 (2) 0.12
Polar flagellin F OS=Vibrio parahaemolyticus GN=flaF PE=3 SV=2
FLAA_VIBAN 67 40087 2 (2) 2 (2) 0.12
Flagellin A OS=Vibrio anguillarum GN=flaA PE=3 SV=1
FLAA_VIBCH 67 40358 2 (2) 2 (2) 0.12
Flagellin A OS=Vibrio cholerae GN=flaA PE=3 SV=1
FLAC_VIBPA 67 40787 2 (2) 2 (2) 0.12
Polar flagellin C OS=Vibrio parahaemolyticus GN=flaC PE=3 SV=2
FLAD_VIBAN 67 39617 2 (2) 2 (2) 0.12
Flagellin D OS=Vibrio anguillarum GN=flaD PE=1 SV=3
FLICB_PSEAE 67 49213 2 (2) 2 (2) 0.10
B-type flagellin OS=Pseudomonas aeruginosa GN=fliC PE=1 SV=2
FLAA_VIBC3 67 40358 2 (2) 2 (2) 0.12
Flagellin A OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) GN=flaA PE=3 SV=1
FLAC_VIBCH 67 39881 2 (2) 2 (2) 0.12
Flagellin C OS=Vibrio cholerae GN=flaC PE=3 SV=1
FLAD_VIBCH 67 39880 2 (2) 2 (2) 0.12
Flagellin D OS=Vibrio cholerae GN=flaD PE=1 SV=1
FLAC_VIBC3 67 39881 2 (2) 2 (2) 0.12
Flagellin C OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) GN=flaC PE=3 SV=1
FLAD_VIBC3 67 39880 2 (2) 2 (2) 0.12
Flagellin D OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) GN=flaD PE=3 SV=1

+5 peptide matches (4 non-duplicate, 1 duplicate)

1 subset or intersection (4 subset proteins in total)

Score Mass Subset of
FLAB_VIBAN 51 39509 94.1
Flagellin B OS=Vibrio anguillarum GN=flaB PE=1 SV=3
3 samesets of FLAB_VIBAN
FLAB_VIBCH 51 39492
Flagellin B OS=Vibrio cholerae GN=flaB PE=3 SV=1
FLAB_VIBC3 51 39492
Flagellin B OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) GN=flaB PE=3 SV=1
FLAC_VIBAN 51 40076
Flagellin C OS=Vibrio anguillarum GN=flaC PE=1 SV=3

+95

Accession Score Description
1 CLPB_PSEPK 65 Chaperone protein clpB OS=Pseudomonas putida (strain KT2440) GN=clpB PE=3 SV=1

+96

Accession Score Description
Family member distances as a dendrogram 1 XYLA_ECOBW 65 Xylose isomerase OS=Escherichia coli (strain K12 / BW2952) GN=xylA PE=3 SV=1
2 XYLA_ENT38 53 Xylose isomerase OS=Enterobacter sp. (strain 638) GN=xylA PE=3 SV=1

+97

Accession Score Description
1 RS6_AZOVD 64 30S ribosomal protein S6 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsF PE=3 SV=1

+98

Accession Score Description
1 PURA_PSEPG 64 Adenylosuccinate synthetase OS=Pseudomonas putida (strain GB-1) GN=purA PE=3 SV=1

+99

Accession Score Description
1 SUCC_COXBN 63 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Coxiella burnetii (strain Dugway 5J108-111) GN=sucC PE=3 SV=1

+100

Accession Score Description
1 ATP6_PSEP1 63 ATP synthase subunit a OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=atpB PE=3 SV=1
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