MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 sp |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 22:50:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.
1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPB_PSEPG | 1648 | ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1 | |
| 2 | ATPB_LEGPA | 425 | ATP synthase subunit beta OS=Legionella pneumophila (strain Paris) GN=atpD PE=3 SV=1 | |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTU2_PSEPK | 1599 | Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1 | |
| 5 | EFTU_MYCS5 | 42 | Elongation factor Tu OS=Mycoplasma synoviae (strain 53) GN=tuf PE=3 SV=1 | |
| 3 | EFTU_CARRP | 146 | Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1 | |
| 4 | EFTU_ANATD | 54 | Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1 | |
| 2 | EFTU1_PSEPK | 1484 | Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1 | |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPA_PSEPK | 907 | ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1 | |
| 3 | ATPA_RICAH | 176 | ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1 | |
| 2 | ATPA_VEREI | 178 | ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1 | |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PORF_PSESY | 690 | Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1 | |
| 2 | PORF_PSEAE | 143 | Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1 | |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOB_PSEPG | 513 | DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1 | |
| 5 | FTSZ_PSEPK | 129 | Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3 | |
| 2 | RPOC_PSEP1 | 418 | DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoC PE=3 SV=1 | |
| 3 | RPOC_PSEU5 | 335 | DNA-directed RNA polymerase subunit beta' OS=Pseudomonas stutzeri (strain A1501) GN=rpoC PE=3 SV=1 | |
| 6 | RPOC_BIFLD | 91 | DNA-directed RNA polymerase subunit beta' OS=Bifidobacterium longum (strain DJO10A) GN=rpoC PE=3 SV=1 | |
| 4 | RPOC_SYNAS | 202 | DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1 | |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS5_PSEE4 | 428 | 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL3_PSEP1 | 378 | 50S ribosomal protein L3 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplC PE=3 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ARPC_PSEPU | 369 | Antibiotic efflux pump outer membrane protein arpC OS=Pseudomonas putida GN=arpC PE=2 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL2_PSEP1 | 329 | 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 9.1 | RL2_PSEP1 |
329 | 29822 | 23 (12) | 12 (6) | 0.82 | |
| 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1 | |||||||
| 4 samesets of RL2_PSEP1 | |||||||
| RL2_PSEPK |
329 | 29822 | 23 (12) | 12 (6) | 0.82 | ||
| 50S ribosomal protein L2 OS=Pseudomonas putida (strain KT2440) GN=rplB PE=3 SV=1 | |||||||
| RL2_PSEPW |
329 | 29822 | 23 (12) | 12 (6) | 0.82 | ||
| 50S ribosomal protein L2 OS=Pseudomonas putida (strain W619) GN=rplB PE=3 SV=1 | |||||||
| RL2_PSEE4 |
329 | 29808 | 19 (12) | 11 (6) | 0.82 | ||
| 50S ribosomal protein L2 OS=Pseudomonas entomophila (strain L48) GN=rplB PE=3 SV=1 | |||||||
| RL2_PSEPG |
329 | 29794 | 18 (12) | 10 (6) | 0.82 | ||
| 50S ribosomal protein L2 OS=Pseudomonas putida (strain GB-1) GN=rplB PE=3 SV=1 | |||||||
23 peptide matches (18 non-duplicate, 5 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 321 |
615.3600 | 614.3527 | 614.3500 | 4.45 | 0 | 27 | 1 | 7Score > 40 indicates identity |
K.GAQIAR.S | ||
| 372 |
325.1879 | 648.3612 | 648.3595 | 2.68 | 0 | 20 | 0.098 | 1Score > 40 indicates identity Score > 23 indicates homology |
R.LVDFR.R | ||
| 484 |
352.7217 | 703.4288 | 703.4268 | 2.86 | 0 | 30 | 0.12 | 1Score > 35 indicates identity Score > 33 indicates homology |
U | R.YIIAPK.G | |
| 485 |
704.4369 | 703.4296 | 703.4268 | 3.97 | 0 | 40 | 0.017 | 1Score > 35 indicates identity |
U | R.YIIAPK.G | |
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_1_20250714114344.rawScore > 36 indicates identity Score > 32 indicates homology |
|||||||||||
| 643 |
392.7442 | 783.4738 | 783.4715 | 2.96 | 0 | 26 | 0.21 | 1Score > 36 indicates identity Score > 32 indicates homology |
R.GVRPTVR.G | ||
| 2.98 | 1 | 18 | 1.4 | 2 | RGVPSLR | ||||||
| -11.3 | 1 | 18 | 1.4 | 2 | RRPISR | ||||||
| -11.3 | 1 | 18 | 1.4 | 2 | RRPLSR | ||||||
| -11.3 | 1 | 18 | 1.4 | 2 | RRPSIR | ||||||
| 17.3 | 0 | 18 | 1.4 | 6 | IAQTPVR | ||||||
| 17.3 | 0 | 16 | 2.3 | 7 | AAIGTPVR | ||||||
| 17.3 | 0 | 16 | 2.3 | 7 | TNPVVVR | ||||||
| 17.4 | 1 | 14 | 3.1 | 9 | KAIPQAR + Deamidated (NQ) | ||||||
| 17.3 | 0 | 14 | 3.1 | 9 | QAIPISR | ||||||
| 818 |
429.7447 | 857.4748 | 857.4719 | 3.45 | 0 | 44 | 0.013 | 1Score > 43 indicates identity Score > 38 indicates homology |
U | K.AGNSLQLR.N | |
| 925 |
453.7201 | 905.4256 | 905.4243 | 1.52 | 1 | 39 | 0.065 | 1Score > 42 indicates identity Score > 39 indicates homology |
R.IEYDPNR.T | ||
| 996 |
468.7626 | 935.5106 | 935.5076 | 3.23 | 1 | 20 | 1.4 | 3Score > 40 indicates identity Score > 34 indicates homology |
U | R.EGVYVTLR.L | |
| 1213 |
1 |
344.8671 | 1031.5795 | 1031.5764 | 3.02 | 1 | 34 | 0.05 | 1Score > 40 indicates identity Score > 34 indicates homology |
U | K.GAPHAPLIEK.K |
| 1214 |
516.7978 | 1031.5810 | 1031.5764 | 4.55 | 1 | 38 | 0.012 | 1Score > 40 indicates identity Score > 31 indicates homology |
U | K.GAPHAPLIEK.K | |
| 1243 |
1 |
522.7986 | 1043.5826 | 1043.5723 | 9.89 | 0 | 54 | 0.0007 | 1Score > 40 indicates identity Score > 35 indicates homology |
U | R.SAGASAQLIAR.E |
| 1698 |
1 |
626.8296 | 1251.6446 | 1251.6401 | 3.67 | 0 | 66 | 4.7e-005 | 1Score > 40 indicates identity Score > 35 indicates homology |
U | R.HPVSPWGFPTK.G |
| 2354 |
2 |
790.9595 | 1579.9044 | 1579.8933 | 7.03 | 0 | 91 | 1.2e-007 | 1Score > 34 indicates identity |
U | K.GVSAGDQLIAGALAPIK.A |
| 2355 |
527.6426 | 1579.9060 | 1579.8933 | 7.99 | 0 | 61 | 3e-005 | 1Score > 34 indicates identity Score > 28 indicates homology |
U | K.GVSAGDQLIAGALAPIK.A | |
| 2753 |
587.3401 | 1758.9985 | 1758.9992 | -0.42 | 1 | 23 | 0.081 | 1Score > 33 indicates identity Score > 25 indicates homology |
U | R.NIPVGSTIHGIELKPGK.G | |
| 2754 |
440.7589 | 1759.0065 | 1758.9992 | 4.14 | 1 | 13 | 0.77 | 1Score > 32 indicates identity Score > 24 indicates homology |
U | R.NIPVGSTIHGIELKPGK.G | |
| 2756 |
441.0092 | 1760.0077 | 1759.9832 | 13.9 | 1 | 14 | 0.089 | 1Score > 32 indicates identity Score > 16 indicates homology |
U | R.NIPVGSTIHGIELKPGK.G + Deamidated (NQ) | |
| 2757 |
587.6773 | 1760.0101 | 1759.9832 | 15.3 | 1 | 10 | 0.18 | 1Score > 32 indicates identity Score > 15 indicates homology |
U | R.NIPVGSTIHGIELKPGK.G + Deamidated (NQ) | |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| RL2_PSE14 |
129 | 29789 | 9.1 | ||
| 50S ribosomal protein L2 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplB PE=3 SV=1 | |||||
| 4 samesets of RL2_PSE14 | |||||
| RL2_PSEFS |
129 | 29803 | |||
| 50S ribosomal protein L2 OS=Pseudomonas fluorescens (strain SBW25) GN=rplB PE=3 SV=1 | |||||
| RL2_PSEPF |
129 | 29773 | |||
| 50S ribosomal protein L2 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplB PE=3 SV=1 | |||||
| RL2_PSESM |
129 | 29833 | |||
| 50S ribosomal protein L2 OS=Pseudomonas syringae pv. tomato GN=rplB PE=3 SV=1 | |||||
| RL2_PSEU2 |
129 | 29789 | |||
| 50S ribosomal protein L2 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplB PE=3 SV=1 | |||||
| RL2_PSEMY |
122 | 29847 | 9.1 | ||
| 50S ribosomal protein L2 OS=Pseudomonas mendocina (strain ymp) GN=rplB PE=3 SV=1 | |||||
| RL2_PSEF5 |
100 | 29745 | 9.1 | ||
| 50S ribosomal protein L2 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplB PE=3 SV=1 | |||||
| RL2_AZOVD |
93 | 29689 | 9.1 | ||
| 50S ribosomal protein L2 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rplB PE=3 SV=1 | |||||
| 1 sameset of RL2_AZOVD | |||||
| RL2_PSEU5 |
93 | 29761 | |||
| 50S ribosomal protein L2 OS=Pseudomonas stutzeri (strain A1501) GN=rplB PE=3 SV=1 | |||||
| RL2_NEOSM |
86 | 30311 | 9.1 | ||
| 50S ribosomal protein L2 OS=Neorickettsia sennetsu (strain Miyayama) GN=rplB PE=3 SV=1 | |||||
| RL2_RALPJ |
46 | 30251 | 9.1 | ||
| 50S ribosomal protein L2 OS=Ralstonia pickettii (strain 12J) GN=rplB PE=3 SV=1 | |||||
| 2 samesets of RL2_RALPJ | |||||
| RL2_SACD2 |
46 | 30282 | |||
| 50S ribosomal protein L2 OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=rplB PE=3 SV=1 | |||||
| RL2_RALSO |
46 | 30198 | |||
| 50S ribosomal protein L2 OS=Ralstonia solanacearum GN=rplB PE=3 SV=1 | |||||
| RK2_RHDSA |
40 | 30508 | 9.1 | ||
| 50S ribosomal protein L2, chloroplastic OS=Rhodomonas salina GN=rpl2 PE=3 SV=1 | |||||
| 258 samesets of RK2_RHDSA |
|||||
| RL2_RALEH |
39 | 30215 | 9.1 | ||
| 50S ribosomal protein L2 OS=Ralstonia eutropha (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) GN=rplB PE=3 SV=1 | |||||
| 2 samesets of RL2_RALEH | |||||
| RL2_RALEJ |
39 | 30053 | |||
| 50S ribosomal protein L2 OS=Ralstonia eutropha (strain JMP134) GN=rplB PE=3 SV=1 | |||||
| RL2_RALME |
39 | 30115 | |||
| 50S ribosomal protein L2 OS=Ralstonia metallidurans (strain CH34 / ATCC 43123 / DSM 2839) GN=rplB PE=3 SV=1 | |||||
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DLDH2_PSEPU | 325 | Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4 | |
| 2 | DLDH_AZOVI | 161 | Dihydrolipoyl dehydrogenase OS=Azotobacter vinelandii PE=1 SV=1 | |
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