MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 81–90 (out of 230)

Page: Previous 1 …4 5 6 7 8 9 10 11 12 13 14 … 23 Next 

+81

Accession Score Description
1 GYRB_PSEPU 80 DNA gyrase subunit B OS=Pseudomonas putida GN=gyrB PE=3 SV=2

+82

Accession Score Description
1 ACP_PSEPK 79 Acyl carrier protein OS=Pseudomonas putida (strain KT2440) GN=acpP PE=3 SV=1

+83

Accession Score Description
1 RS7_DESDG 78 30S ribosomal protein S7 OS=Desulfovibrio desulfuricans (strain G20) GN=rpsG PE=3 SV=1

+84

Accession Score Description
1 RS15_PSEPG 78 30S ribosomal protein S15 OS=Pseudomonas putida (strain GB-1) GN=rpsO PE=3 SV=1

+85

Accession Score Description
1 RL7_PSEP1 77 50S ribosomal protein L7/L12 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplL PE=3 SV=1

+86

Accession Score Description
1 ETFB_PSEAE 76 Electron transfer flavoprotein subunit beta OS=Pseudomonas aeruginosa GN=etfB PE=3 SV=1

+87

Accession Score Description
1 IDH_AZOVI 76 Isocitrate dehydrogenase [NADP] OS=Azotobacter vinelandii GN=icd PE=1 SV=5

-88

Accession Score Description
1 ODP2_PSEAE 75 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas aeruginosa GN=aceF PE=3 SV=2
Score Mass Matches Sequences emPAI
88.1 ODP2_PSEAE 75 56788 3 (2) 3 (2) 0.08
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas aeruginosa GN=aceF PE=3 SV=2

-3 peptide matches (3 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
201   473.3104 472.3031 472.3009 4.64 0 23 0.24 +1Score > 45 indicates identity
Score > 29 indicates homology		 K.AGVVK.S
1171   505.3573 1008.7000 1008.6947 5.31 0 41 0.00012 +1Score > 15 indicates identity U K.LTVLPILLK.A
2095   731.3697 1460.7248 1460.7147 6.94 1 55 9.4e-005 +1Score > 41 indicates identity
Score > 27 indicates homology		 U K.ELPDFNSSLAPSGK.A

1 subset or intersection (1 subset protein in total)

Score Mass Subset of
ODP2_AZOVI 41 65119 88.1
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Azotobacter vinelandii PE=1 SV=3

+89

Accession Score Description
1 PCAF_PSEPU 74 Beta-ketoadipyl-CoA thiolase OS=Pseudomonas putida GN=pcaF PE=3 SV=1

+90

Accession Score Description
1 ODBB_PSEPU 74 2-oxoisovalerate dehydrogenase subunit beta OS=Pseudomonas putida GN=bkdA2 PE=1 SV=1
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