MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 sp |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 22:50:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.
1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPB_PSEPG | 1648 | ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1 | |
| 2 | ATPB_LEGPA | 425 | ATP synthase subunit beta OS=Legionella pneumophila (strain Paris) GN=atpD PE=3 SV=1 | |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTU2_PSEPK | 1599 | Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1 | |
| 5 | EFTU_MYCS5 | 42 | Elongation factor Tu OS=Mycoplasma synoviae (strain 53) GN=tuf PE=3 SV=1 | |
| 3 | EFTU_CARRP | 146 | Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1 | |
| 4 | EFTU_ANATD | 54 | Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1 | |
| 2 | EFTU1_PSEPK | 1484 | Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1 | |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPA_PSEPK | 907 | ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1 | |
| 3 | ATPA_RICAH | 176 | ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1 | |
| 2 | ATPA_VEREI | 178 | ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1 | |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PORF_PSESY | 690 | Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1 | |
| 2 | PORF_PSEAE | 143 | Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1 | |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOB_PSEPG | 513 | DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1 | |
| 5 | FTSZ_PSEPK | 129 | Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3 | |
| 2 | RPOC_PSEP1 | 418 | DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoC PE=3 SV=1 | |
| 3 | RPOC_PSEU5 | 335 | DNA-directed RNA polymerase subunit beta' OS=Pseudomonas stutzeri (strain A1501) GN=rpoC PE=3 SV=1 | |
| 6 | RPOC_BIFLD | 91 | DNA-directed RNA polymerase subunit beta' OS=Bifidobacterium longum (strain DJO10A) GN=rpoC PE=3 SV=1 | |
| 4 | RPOC_SYNAS | 202 | DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1 | |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS5_PSEE4 | 428 | 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL3_PSEP1 | 378 | 50S ribosomal protein L3 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplC PE=3 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ARPC_PSEPU | 369 | Antibiotic efflux pump outer membrane protein arpC OS=Pseudomonas putida GN=arpC PE=2 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 8.1 | ARPC_PSEPU |
369 | 52859 | 10 (10) | 9 (9) | 0.53 | |
| Antibiotic efflux pump outer membrane protein arpC OS=Pseudomonas putida GN=arpC PE=2 SV=1 | |||||||
| 3 samesets of ARPC_PSEPU | |||||||
| MEPC_PSEPU |
369 | 52889 | 10 (10) | 9 (9) | 0.53 | ||
| Multidrug/solvent efflux pump outer membrane protein mepC OS=Pseudomonas putida GN=mepC PE=1 SV=1 | |||||||
| TTGC_PSEPK |
369 | 52889 | 10 (10) | 9 (9) | 0.53 | ||
| Probable efflux pump outer membrane protein ttgC OS=Pseudomonas putida (strain KT2440) GN=ttgC PE=1 SV=1 | |||||||
| TTGC_PSEPU |
369 | 52873 | 10 (10) | 9 (9) | 0.53 | ||
| Toluene efflux pump outer membrane protein ttgC OS=Pseudomonas putida GN=ttgC PE=2 SV=1 | |||||||
10 peptide matches (10 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 978 |
464.7527 | 927.4908 | 927.4886 | 2.44 | 0 | 52 | 0.002 | 1Score > 40 indicates identity Score > 38 indicates homology |
U | K.AANANIGAAR.A | |
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_1_20250714114344.rawScore > 40 indicates identity Score > 37 indicates homology |
|||||||||||
| 1941 |
692.8296 | 1383.6446 | 1383.6419 | 2.00 | 0 | 42 | 0.018 | 1Score > 40 indicates identity Score > 37 indicates homology |
U | R.DLVQANQDYYR.L | |
| 10.1 | 1 | 22 | 1.8 | 2 | LYFDDEAQANAK | ||||||
| -16.2 | 1 | 9 | 36 | 3 | NNVAQLKFYSAE + Deamidated (NQ) | ||||||
| -5.13 | 1 | 7 | 56 | 4 | LNLNAPSQGDPKE + 2 Deamidated (NQ) | ||||||
| -8.06 | 0 | 7 | 60 | 5 | IDVFDNSQLFGK + 2 Deamidated (NQ) | ||||||
| 12.4 | 1 | 7 | 61 | 6 | YAQVQAAMEMAR + Oxidation (M) | ||||||
| -16.2 | 1 | 7 | 61 | 7 | LDVPENAAVWAAE | ||||||
| -13.3 | 1 | 7 | 62 | 8 | IDVNTSHNKLNE + Deamidated (NQ) | ||||||
| -16.2 | 1 | 6 | 73 | 9 | LVNLYFGDGRTE + Deamidated (NQ) | ||||||
| 2.32 | 1 | 6 | 74 | 10 | VVPDFKSAMMVE + 2 Oxidation (M) | ||||||
| 1969 |
697.8825 | 1393.7504 | 1393.7453 | 3.72 | 1 | 82 | 8.1e-007 | 1Score > 38 indicates identity Score > 33 indicates homology |
U | R.LSQLTSEVNLYK.A | |
| 2053 |
716.8865 | 1431.7584 | 1431.7470 | 7.99 | 0 | 64 | 0.0002 | 1Score > 40 indicates identity |
U | R.NLFSAQQALIGDR.L | |
| 2057 |
718.3665 | 1434.7184 | 1434.7103 | 5.67 | 0 | 45 | 0.025 | 1Score > 41 indicates identity |
U | R.ADLFPAVSATGSGSR.Q | |
| 2314 |
781.9204 | 1561.8262 | 1561.8100 | 10.4 | 0 | 117 | 7.7e-010 | 1Score > 40 indicates identity Score > 39 indicates homology |
U | R.IGIDSNLTFLDAQR.N | |
| 2395 |
801.9103 | 1601.8060 | 1601.8009 | 3.21 | 1 | 89 | 1.2e-007 | 1Score > 41 indicates identity Score > 32 indicates homology |
U | R.SNEVGVASALDVSQAR.T | |
| 2643 |
569.6328 | 1705.8766 | 1705.8635 | 7.66 | 1 | 94 | 3.5e-008 | 1Score > 40 indicates identity Score > 32 indicates homology |
U | K.TIQTAFQEVSDGLAAR.K | |
| 2644 |
854.4401 | 1706.8656 | 1706.8475 | 10.6 | 1 | 51 | 0.00016 | 1Score > 40 indicates identity Score > 25 indicates homology |
U | K.TIQTAFQEVSDGLAAR.K + Deamidated (NQ) | |
| 3789 |
790.4212 | 2368.2418 | 2368.2288 | 5.49 | 0 | 36 | 0.0015 | 1Score > 37 indicates identity Score > 21 indicates homology |
U | R.QFFHDPALQQLIQTSLVNNR.D | |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL2_PSEP1 | 329 | 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DLDH2_PSEPU | 325 | Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4 | |
| 2 | DLDH_AZOVI | 161 | Dihydrolipoyl dehydrogenase OS=Azotobacter vinelandii PE=1 SV=1 | |
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