MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 71–80 (out of 230)


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+71

Accession Score Description
1 RL22_PSEA7 96 50S ribosomal protein L22 OS=Pseudomonas aeruginosa (strain PA7) GN=rplV PE=3 SV=1

+72

Accession Score Description
1 ODO1_COXBU 96 2-oxoglutarate dehydrogenase E1 component OS=Coxiella burnetii GN=sucA PE=3 SV=3

+73

Accession Score Description
1 GLNA_AZOVI 95 Glutamine synthetase OS=Azotobacter vinelandii GN=glnA PE=3 SV=1

+74

Accession Score Description
1 KPRS_PSEPK 93 Ribose-phosphate pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=prs PE=3 SV=1

+75

Accession Score Description
1 NUOI_PSEPK 87 NADH-quinone oxidoreductase subunit I OS=Pseudomonas putida (strain KT2440) GN=nuoI PE=3 SV=1

+76

Accession Score Description
1 AROB_PSEPK 85 3-dehydroquinate synthase OS=Pseudomonas putida (strain KT2440) GN=aroB PE=3 SV=1

+77

Accession Score Description
1 RS18_PSEE4 84 30S ribosomal protein S18 OS=Pseudomonas entomophila (strain L48) GN=rpsR PE=3 SV=1

+78

Accession Score Description
1 ARCA_PSEPK 82 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

-79

Accession Score Description
1 ATPE_PSEPG 82 ATP synthase epsilon chain OS=Pseudomonas putida (strain GB-1) GN=atpC PE=3 SV=1
Score Mass Matches Sequences emPAI
79.1 ATPE_PSEPG 82 14606 3 (2) 3 (2) 0.35
ATP synthase epsilon chain OS=Pseudomonas putida (strain GB-1) GN=atpC PE=3 SV=1
2 samesets of ATPE_PSEPG
ATPE_PSEPK 82 14585 3 (2) 3 (2) 0.35
ATP synthase epsilon chain OS=Pseudomonas putida (strain KT2440) GN=atpC PE=3 SV=1
ATPE_PSEPW 82 14595 2 (2) 2 (2) 0.35
ATP synthase epsilon chain OS=Pseudomonas putida (strain W619) GN=atpC PE=3 SV=1

-3 peptide matches (3 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1021   471.7745 941.5344 941.5294 5.37 1 63 0.00022 +1Score > 39 indicates identity U R.LAEAAAQLR.T
1026   472.2664 942.5182 942.5134 5.15 1 23 1.3 +3Score > 41 indicates identity
Score > 37 indicates homology
U K.AAENALNLK.G
1559   592.7721 1183.5296 1183.5258 3.26 0 58 0.00084 +1Score > 39 indicates identity U K.GADFDYGAAAAR.L

1 subset or intersection (20 subset proteins in total)

Score Mass Subset of
ATPE_COXB1 63 15277 79.1
ATP synthase epsilon chain OS=Coxiella burnetii (strain CbuK_Q154) GN=atpC PE=3 SV=1
19 samesets of ATPE_COXB1
ATPE_PSEP1 63 14636
ATP synthase epsilon chain OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=atpC PE=3 SV=1
ATPE_MARAV 63 15117
ATP synthase epsilon chain OS=Marinobacter aquaeolei (strain ATCC 700491 / DSM 11845 / VT8) GN=atpC PE=3 SV=1
ATPE_COXBN 63 15277
ATP synthase epsilon chain OS=Coxiella burnetii (strain Dugway 5J108-111) GN=atpC PE=3 SV=1
ATPE_COXBR 63 15277
ATP synthase epsilon chain OS=Coxiella burnetii (strain RSA 331 / Henzerling II) GN=atpC PE=3 SV=1
ATPE_COXBU 63 15277
ATP synthase epsilon chain OS=Coxiella burnetii GN=atpC PE=3 SV=1
ATPE_PSEAB 63 14774
ATP synthase epsilon chain OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=atpC PE=3 SV=1
ATPE_PSEAE 63 14774
ATP synthase epsilon chain OS=Pseudomonas aeruginosa GN=atpC PE=3 SV=1
ATPE_PSEFS 63 14615
ATP synthase epsilon chain OS=Pseudomonas fluorescens (strain SBW25) GN=atpC PE=3 SV=1
ATPE_PSEMY 63 15370
ATP synthase epsilon chain OS=Pseudomonas mendocina (strain ymp) GN=atpC PE=3 SV=1
ATPE_PSEPF 63 14802
ATP synthase epsilon chain OS=Pseudomonas fluorescens (strain Pf0-1) GN=atpC PE=3 SV=1
ATPE_PSESM 63 14607
ATP synthase epsilon chain OS=Pseudomonas syringae pv. tomato GN=atpC PE=3 SV=1
ATPE_TERTT 63 14938
ATP synthase epsilon chain OS=Teredinibacter turnerae (strain ATCC 39867 / T7901) GN=atpC PE=3 SV=1
ATPE_COXB2 63 15277
ATP synthase epsilon chain OS=Coxiella burnetii (strain CbuG_Q212) GN=atpC PE=3 SV=1
ATPE_PSEU2 63 14794
ATP synthase epsilon chain OS=Pseudomonas syringae pv. syringae (strain B728a) GN=atpC PE=3 SV=1
ATPE_PSEE4 63 14635
ATP synthase epsilon chain OS=Pseudomonas entomophila (strain L48) GN=atpC PE=3 SV=1
ATPE_PSEF5 63 14853
ATP synthase epsilon chain OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=atpC PE=3 SV=1
ATPE_PSEA7 63 14774
ATP synthase epsilon chain OS=Pseudomonas aeruginosa (strain PA7) GN=atpC PE=3 SV=1
ATPE_PSEA8 63 14774
ATP synthase epsilon chain OS=Pseudomonas aeruginosa (strain LESB58) GN=atpC PE=3 SV=1
ATPE_PSE14 63 14794
ATP synthase epsilon chain OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=atpC PE=3 SV=1

+80

Accession Score Description
1 PUR5_PSEP1 80 Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=purM PE=3 SV=1
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