MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1 sp
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 22:50:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 71–80 (out of 230)

Page: Previous 1 …3 4 5 6 7 8 9 10 11 12 13 … 23 Next 

+71

Accession Score Description
1 RL22_PSEA7 96 50S ribosomal protein L22 OS=Pseudomonas aeruginosa (strain PA7) GN=rplV PE=3 SV=1

+72

Accession Score Description
1 ODO1_COXBU 96 2-oxoglutarate dehydrogenase E1 component OS=Coxiella burnetii GN=sucA PE=3 SV=3

+73

Accession Score Description
1 GLNA_AZOVI 95 Glutamine synthetase OS=Azotobacter vinelandii GN=glnA PE=3 SV=1

-74

Accession Score Description
1 KPRS_PSEPK 93 Ribose-phosphate pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=prs PE=3 SV=1
Score Mass Matches Sequences emPAI
74.1 KPRS_PSEPK 93 34456 4 (3) 4 (3) 0.21
Ribose-phosphate pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=prs PE=3 SV=1
1 sameset of KPRS_PSEPK
KPRS_PSESM 93 34412 4 (3) 4 (3) 0.21
Ribose-phosphate pyrophosphokinase OS=Pseudomonas syringae pv. tomato GN=prs PE=3 SV=1

-4 peptide matches (4 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1416   565.3315 1128.6484 1128.6390 8.34 0 36 0.0051 +1Score > 39 indicates identity
Score > 26 indicates homology		 U K.SLGVDLGIIDK.R
1887   454.9321 1361.7745 1361.7667 5.70 0 66 5.1e-005 +1Score > 35 indicates identity U R.QLHIPLGDVSVGK.F
1917   685.8833 1369.7520 1369.7394 9.23 0 15 0.6 +1Score > 39 indicates identity
Score > 25 indicates homology		 R.ITAVIPYFGYAR.Q
1937   690.9055 1379.7964 1379.7773 13.9 1 54 0.00057 +1Score > 34 indicates identity U R.QLDIAPVVAEAVR.R

1 subset or intersection (1 subset protein in total)

Score Mass Subset of
KPRS_PSEAE 66 34247 74.1
Ribose-phosphate pyrophosphokinase OS=Pseudomonas aeruginosa GN=prs PE=3 SV=1

+75

Accession Score Description
1 NUOI_PSEPK 87 NADH-quinone oxidoreductase subunit I OS=Pseudomonas putida (strain KT2440) GN=nuoI PE=3 SV=1

+76

Accession Score Description
1 AROB_PSEPK 85 3-dehydroquinate synthase OS=Pseudomonas putida (strain KT2440) GN=aroB PE=3 SV=1

+77

Accession Score Description
1 RS18_PSEE4 84 30S ribosomal protein S18 OS=Pseudomonas entomophila (strain L48) GN=rpsR PE=3 SV=1

+78

Accession Score Description
1 ARCA_PSEPK 82 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

+79

Accession Score Description
1 ATPE_PSEPG 82 ATP synthase epsilon chain OS=Pseudomonas putida (strain GB-1) GN=atpC PE=3 SV=1

+80

Accession Score Description
1 PUR5_PSEP1 80 Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=purM PE=3 SV=1
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